In vitro permeation studies of phenolics from horse chestnut seed gels prepared with different polyacrylic acid polymer derivatives

Abstract The aim of this study was to investigate the effects of polyacrylic acid polymers (Ultrez 10, Ultrez 20, Carbopol 980, and Carbopol 940) on the viscosity and the in vitro permeation of phenolic compounds from the gel prepared from natural horse chestnut seed extract. Experiments were performed in the presence and in the absence of peppermint oil (Mentha piperita). Our results showed that peppermint oil decreased the viscosity of the gels and permeation of phenolic compounds from all gel samples. Results show that the highest content of phenolic compounds (1.758 μg cm-2) permeated in vitro from gel based on Carbopol Ultrez 20 without peppermint oil added (p < 0.05 vs. other tested polymers).

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Deformable Surfactant Vesicles Loading Ammonium Glycyrrhizinate: Characterization and In Vitro Permeation Studies

Letters in Drug Design & Discovery ◽

10.2174/157018012800389287 ◽

2012 ◽

Vol 9 (5) ◽

pp. 494-499 ◽

Cited By ~ 7

Author(s):

Luisa Di Marzio ◽

Carlotta Marianecci ◽

Federica Rinaldi ◽

Sara Esposito ◽

Maria Carafa

Keyword(s):

Surfactant Vesicles ◽

In Vitro Permeation ◽

Permeation Studies

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Development and evaluation of a heparin gel for transdermal delivery via laser-generated micropores

Therapeutic Delivery ◽

10.4155/tde-2020-0024 ◽

2021 ◽

Vol 12 (2) ◽

pp. 133-144

Author(s):

Deepal Vora ◽

Yujin Kim ◽

Ajay K Banga

Keyword(s):

Transdermal Delivery ◽

Continuous Intravenous Infusion ◽

In Vitro Permeation ◽

Assisted Delivery ◽

Permeation Studies ◽

Franz Diffusion Cells ◽

Transdermal Route ◽

Poloxamer Gel ◽

Ablative Laser

Aim: Our study investigated the feasibility of transdermal delivery of heparin, an anticoagulant used against venous thromboembolism, as an alternative to intravenous administration. Materials & methods: Skin was pretreated using ablative laser (Precise Laser Epidermal System [P.L.E.A.S.E.®] technology) for enhanced delivery of heparin. In vitro permeation studies using static Franz diffusion cells provided a comparison between delivery from 0.3% w/v heparin-loaded poloxamer gel and solution across untreated and laser-treated dermatomed porcine ear skin. Results: No passive delivery of heparin was observed. Laser-assisted delivery from solution (26.07 ± 1.82 μg/cm2) was higher (p < 0.05) than delivery from heparin gel (11.28 ± 5.32 μg/cm2). However, gel is likely to sustain the delivery over prolonged periods like a maintenance dose via continuous intravenous infusion. Conclusion: Thus, ablative laser pretreatment successfully delivered heparin, establishing the feasibility of delivering hydrophilic macromolecules using the transdermal route.

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Limitations of Hairless Mouse Skin as a Model for In Vitro Permeation Studies Through Human Skin: Hydration Damage

Journal of Investigative Dermatology ◽

10.1111/1523-1747.ep12460958 ◽

1988 ◽

Vol 90 (4) ◽

pp. 486-489 ◽

Cited By ~ 81

Author(s):

John Russell. Bond ◽

Brian William. Barry

Keyword(s):

Human Skin ◽

Mouse Skin ◽

Hairless Mouse ◽

Skin Hydration ◽

Hairless Mouse Skin ◽

In Vitro Permeation ◽

Permeation Studies

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Determination of Diclofenac Sodium in Eagle's Minimum Essential Medium with Earle's Balanced Salt Solution

Journal of AOAC International ◽

10.1093/jaoac/86.4.681 ◽

2003 ◽

Vol 86 (4) ◽

pp. 681-684

Author(s):

Patrícia S Lopes ◽

Telma M Kaneko ◽

Carolina Y Takano ◽

Aurea C L Lacerda ◽

Leandro R Latorre ◽

...

Keyword(s):

Salt Solution ◽

Diclofenac Sodium ◽

Reversed Phase ◽

Minimum Essential Medium ◽

In Vitro Permeation ◽

Hydrophilic Drug ◽

Permeation Studies ◽

The Mean

Abstract A validated method was developed for determination of diclofenac sodium, considered a model hydrophilic drug for in vitro permeation studies, in Eagle's Minimum Essential Medium (MEM) with Earle's balanced salt solution. Liquid chromatography was used to determine diclofenac sodium. This method was developed with a reversed-phase column Supercosil LC 18, DB 25 cm × 4.5 mm; the mobile phase was methanol with 3% (v/v) acetic acid–Milli-Q water (74 + 26), and detection was at 283 nm. The detection and quantitation limits were 2.41 × 10–8 and 3.31 × 10–5 μg/μL, respectively. The accuracy within-day (n = 3) and day-to-day (n = 7) was 98.83%; the mean variation coefficient for inter- (n = 7) and intraday precision (n = 3) was 12.20%, thus, not exceeding 15%. This method can be used as an analytical procedure for the determination of diclofenac sodium in MEM for in vitro permeation studies.

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Novel Design Approaches in the Fabrication of Polymeric Microarray Patches via Micromoulding

Micromachines ◽

10.3390/mi11060554 ◽

2020 ◽

Vol 11 (6) ◽

pp. 554 ◽

Cited By ~ 2

Author(s):

Inken Ramöller ◽

Emma McAlister ◽

Abigail Bogan ◽

Ana Cordeiro ◽

Ryan Donnelly

Keyword(s):

Skin Barrier ◽

Systemic Delivery ◽

Porcine Skin ◽

Density Maps ◽

In Vitro Permeation ◽

Wide Range ◽

Significant Difference ◽

Permeation Studies ◽

Novel Design

The focus on novel systems for transdermal delivery of therapeutic agents has increased considerably over recent years, as this administration route comes with many advantages. Polymeric microarray patches (MAPs) are minimally invasive devices that enable systemic delivery of a wide range of drugs by overcoming the outer skin barrier. Conventionally, MAPs fabricated by micromoulding have a low needle density. In this study, the performance of hydrogel-forming MAPs cast using novel industrially manufactured micromoulds with a high needle density (600 needles/0.75 cm2) was compared to that of MAPs obtained using conventional moulds with a lower density (196 needles/0.89 cm2). Surrounding holders for micromoulds were designed for time-efficient fabrication of MAPs. The influence of needle densities on mechanical strength, insertion efficiency and in vitro permeation of ibuprofen sodium (IBU) was analysed. Insertion of both MAPs into an artificial skin model and neonatal porcine skin was comparable. No significant difference was observed in permeation studies of IBU (p > 0.05), with a delivery of 8.7 ± 1.7 mg for low-density and 9.5 ± 0.1 mg for high-density MAPs within 24 h. This highlights the potential of these novel micromoulds for manufacturing polymeric MAPs with a higher needle density for future applications.

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