scholarly journals Effect of alkan-1-ols on the structure of dopc model membrane

2017 ◽  
Vol 64 (2) ◽  
pp. 4-8 ◽  
Author(s):  
T. Kondela ◽  
J. Gallová ◽  
T. Hauß ◽  
O. Ivankov ◽  
N. Kučerka ◽  
...  
Keyword(s):  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Model Membrane ◽  
Molar Ratio ◽  
Bilayer Thickness ◽  
Oriented Sample ◽  
Repeat Distance ◽  
Lamellar Phases ◽  
Fluid Bilayers ◽  
Thickness Parameter

AbstractThe effect of general anaesthetics alkan-1-ols (CnOH, where n = 10, 12, 14, 16 and 18 is the number of carbon atoms in the molecule) on the structure of dioleoylphosphatidylcholine (DOPC) model membrane was studied by small-angle neutron scattering (SANS) and small-angle neutron diffraction (SAND). Fluid bilayers were prepared at CnOH:DOPC = 0.3 molar ratio. The results of both the experiments show that bilayer thickness - a thickness parameter dg in the case of SANS and lamellar repeat distance D in the case of SAND - increases with increasing n. A coexistence of two lamellar phases with different D was detected by measuring the C18OH+DOPC oriented sample.

scholarly journals Low-pH induced reversible reorganizations of chloroplast thylakoid membranes - as revealed by small-angle neutron scattering

2016 ◽  
Author(s):  
Renáta Ünnep ◽  
Ottó Zsiros ◽  
Zsolt Hörcsik ◽  
Márton Markó ◽  
Anjana Jajoo ◽  
...  
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Azimuthal Angle ◽  
Thylakoid Membranes ◽  
Low Ph ◽  
Regulatory Mechanisms ◽  
Photochemical Quenching ◽  
List Type ◽  
Repeat Distance

AbstractEnergization of thylakoid membranes brings about the acidification of the lumenal aqueous phase, which activates important regulatory mechanisms. Earlier Jajoo and coworkers (2014 FEBS Lett. 588:970) have shown that low pH in isolated plant thylakoid membranes induces changes in the excitation energy distribution between the two photosystems. In order to elucidate the structural background of these changes, we used small-angle neutron scattering on thylakoid membranes exposed to low p2H and show that gradually lowering the p2H from 8.0 to 5.0 causes small but well discernible reversible diminishment of the periodic order and the lamellar repeat distance and an increased mosaicity – similar to the effects elicited by light-induced acidification of the lumen. Our data strongly suggest that thylakoids dynamically respond to the membrane energization and actively participate in different regulatory mechanisms.HighlightsThylakoid membranes exposed to low p2H studied by small-angle neutron scatteringAcidification causes reversible shrinkage and diminished lamellar orderSANS changes induced by low pH resemble those due to light-induced lumenal acidificationAbbreviationsNPQnon-photochemical quenchingqEthe energy-dependent component of NPQΔμH+transmembrane electrochemical potential gradientPSIphotosystem IPSIIphotosystem IILETlinear electron transportCDcircular dichroismSANSsmall-angle neutron scatteringqscattering vector or momentumtransferIintensityq*center position of the Bragg peakRDrepeat distanceφazimuthal angleI(φ)angular dependency of the scattering intensityFWHMfull width at half maximum

Investigations on Fractal Nanostructure of Zinc Oxide by Small Angle Neutron Scattering (SANS)

2014 ◽  
Vol 895 ◽  
pp. 531-534
Author(s):  
Rehana Razali ◽  
Edy. Giri Rachman Putra ◽  
Abdul Aziz Mohamed ◽  
Wan Haliza Abd Majid ◽  
W.A.T. Wan Abdullah ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Primary Particle ◽  
Morphological Structure ◽  
Molar Ratio ◽  
X Ray Diffraction ◽  
X Ray ◽  
Field Emission Electron Microscopy

The zinc oxide nanoparticles were synthesized by solvothermal method for 18 h at150 ̊ C with presence of DEA. The molar ratio of Zn2+solution and DEA was fixed at 1:1. The nanostructure was studied using Small Angle Neutron Scattering (SANS) in BATAN, Indonesia. From calculation, it shows that the material has surface fractal with primary particle size of 25 nm and agglomerate size of ̴ 200 nm. X-ray diffraction (XRD) was done and the the crystallite size was calculated using Scherrer equation. Field emission electron microscopy (FESEM) was done to observe the morphological structure. It shows that the sphere-like agglomerates construct of primary nanoparticles of ZnO.

Small-angle neutron scattering study of the n-decane effect on the bilayer thickness in extruded unilamellar dioleoylphosphatidylcholine liposomes

2000 ◽  
Vol 88 (1-3) ◽  
pp. 165-170 ◽  
Author(s):  
Daniela Uhrı́ková ◽  
Pavol Balgavý ◽  
Norbert Kučerka ◽  
Akhmed Islamov ◽  
Valentin Gordeliy ◽  
...  
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Bilayer Thickness ◽  
Scattering Study ◽  
Neutron Scattering Study

scholarly journals Formation of unilamellar dipalmitoylphosphatidylcholine vesicles promoted by Ca2+ions: A small-angle neutron scattering study

2007 ◽  
Vol 21 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Daniela Uhríková ◽  
José Teixeira ◽  
Adrián Lengyel ◽  
László Almásy ◽  
Pavol Balgavý
Keyword(s):  
Neutron Scattering ◽  
Lipid Bilayer ◽  
Small Angle ◽  
Crystalline Phase ◽  
Small Angle Neutron Scattering ◽  
Liquid Crystalline ◽  
Liquid Crystalline Phase ◽  
Bilayer Thickness ◽  
Unilamellar Vesicles ◽  
Gel Phase

Dipalmitoylphosphatidylcholine (DPPC) was hydrated in 0.2–60 mM solution of CaCl2in heavy water and thoroughly homogenized by freezing-thawing process. Small-angle neutron scattering (SANS) shows formation of unilamellar vesicles in the range 1–60 mM of CaCl2. From the Kratky–Porod plot ln [I(Q)Q2] vs.Q2of SANS intensityI(Q)in the range of scattering vectorsQcorresponding to the interval 0.001 Å−2≤Q2≤0.006 Å−2, the vesicle bilayer radius of gyrationRgand the bilayer thickness parameterdgwere obtained. The structure of the bilayer displays different behavior for the gel phase and the liquid-crystalline phase: In the gel phase (at 20°C), the values ofdgindicate nonlinear changes in the lipid bilayer thickness, with a maximum at ~5 mM CaCl2. In the liquid-crystalline phase (at 60°C), the parameter of the lipid bilayer thicknessdg=43.2±0.3 Å is constant within the concentration range 1≤cCa≤40 mM. Vesicles prepared at 60 mM CaCl2show within experimental error, the same values ofdgas pure DPPC unilamellar vesicles prepared by extrusion using polycarbonate filter with pores of diameter 500 Å.

Undulations in Salt-Free Charged Lamellar Phases Detected by Small Angle Neutron Scattering and Neutron Reflectivity†

Langmuir ◽  
2000 ◽  
Vol 16 (1) ◽  
pp. 102-107 ◽  
Author(s):  
Golchi Salamat ◽  
Renko de Vries ◽  
Eric W. Kaler ◽  
Sushil Satija ◽  
Lipiin Sung
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Neutron Reflectivity ◽  
Lamellar Phases
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