scholarly journals The in vitro shoot regeneration of Ehretia asperula Zol. and Mor.

2019 ◽  
Vol 2 (6) ◽  
pp. 75-83
Author(s):  
Le Thi Thuy Tien
Keyword(s):  
Woody Plant ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
Shoot Initiation ◽  
In Vitro Shoots ◽  
Woody Plant Medium ◽  
Number Of Leaves ◽  
Growth Experiments ◽  
In Vitro Shoot Regeneration

Xa den young branches in the orchard were sterilized and used as explants for shoot initiation and growth experiments. The shoot induction was carried out with BA (benzyl adenine) or TDZ (thidiazuron). New shoots sprouted after one week of culture and the highest shoots (2.02 cm) were on MS medium (Murashige and Skoog medium) with BA 0.6 mg/L after 3 weeks. Furthermore, the number of leaves per shoot was also higher than other treatments (8.31 leaves per shoot). In vitro shoots were used in other experiments to investigate the effects of explants, biotin concentrations, minerals, type and concentration of cytokinins on the formation and elongation of shoots and clusters. When 5 mg/L biotin was added to MS medium, shoots grew better. The MS medium appeared to be most suitable for the initiation and elongating of shoots, followed by WPM (woody plant medium) and SH medium (Schenk and Hildebrandt medium), while B5 medium (Gamborg B5 medium) was the least effective. The spouting from three-week-old explants was earlier than others (4 and 5 weeks of age), which in turn affected on the shoot elongating. BA 1.5 mg/L was suitable to induce shoot clusters (3.91 shoots per explant) after 4 weeks.

scholarly journals PERBANYAKAN AKASIA HIBRIDA (Acacia mangium × Acacia auriculiformis) MELALUI SUBKULTUR BERULANG

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Yelnititis Yelnititis ◽  
Sri Sunarti
Keyword(s):  
Woody Plant ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Acacia Auriculiformis ◽  
Shoot Induction ◽  
Benzyl Adenine ◽  
Single Node ◽  
Woody Plant Medium

In vitro culture is a promising technique for mass propagation of high-value species. Study of propagation for Acacia hybrid (A. mangium x A. auriculiformis) through this technique has been conducted using single node stem from seedlings as explants. Growth medium used was modified Murashige and Skoog (MS), basal medium Woody Plant Medium (WPM), and Gamborg (B5) supplemented. The study was conducted in two stages, namely shoot induction and shoot multiplication. The treatment tested was the Benzyl Adenine (BA) supplementation at the concentration of 0.3; 0.7; and 1.0 mgL-1 of. Observation was conducted on the frequency of shoot induction, number of shoot, shoot length and visual performance of the culture. The result showed that treatment of BA 0.7 mgL-1 on modified MS medium is the best for shoot induction, shoot multiplication and visual performace of the culture. The average of number of shoot was 2.6; 5.0 and 7.7 shoots on the first three consecutive subcultures. Changing to different basal medium on the fourth subculture showed that the treatment of BA 0.7 mgl-1 is the best condition for shoot regeneration (12.60 shoots) and shoot length (6.97 cm). The culture from this treatment showed the best visual morphological performance.Keywords:Acacia hybrid; multiplication; subculture; in vitro; BA. ABSTRAKKultur in vitro merupakan suatu teknik yang menjanjikan untuk perbanyakan massal spesies-tanaman bernilai tinggi. Penelitian perbanyakan akasia hibrid (A. mangium x A. auriculiformis) melalui kulturin vitro telah dilakukan dengan menggunakan eksplan berupa batang satu buku yang berasal dari anakan. Media tumbuh yang digunakan adalah media dasar Murashige dan Skoog (MS) yang sudah dimodifikasi, media dasar Woody Plant Medium (WPM), dan Gamborg (B5). Penelitian dilakukan dalam dua tahap yaitu induksi tunas dan perbanyakan tunas. Perlakuan yang diuji adalah penggunaan Benzyl Adenine (BA) dengan konsentrasi 0,3; 0,7 dan 1,0 mg L-1. Pengamatan dilakukan terhadap waktu induksi tunas, jumlah tunas, tinggi tunas dan penampilan biakan secara visual. Hasil penelitian menunjukkan bahwa penggunaan BA 0,7 mg L-1 pada media MS modifikasi merupakan perlakuan terbaik untuk induksi tunas, perbanyakan tunas, tinggi tunas, dan kondisi biakan secara visual. Jumlah rata-rata tunas yang dihasilkan dari perlakuan ini adalah 2,6; 5,0 dan 7,7 tunas pada subkultur pertama, kedua dan ketiga. Pada penggunaan media dasar berbeda pada subkultur keempat menunjukkan bahwa perlakuan BA 0,7 mg L-1 merupakan perlakuan terbaik dengan jumlah tunas sebanyak 12,60 tunas dan rata-rata tinggi tunas 6,97 cm. Biakan yang dihasilkan dari perlakuan tersebut mempunyai penampilan yang baik dan normal.

scholarly journals Micropropagation of the Rare Lakeside Daisy (Hymenoxys acaulis var. glabra)

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 200-201 ◽  
Author(s):  
James R. Ault
Keyword(s):  
Overall Survival ◽  
Butyric Acid ◽  
Potassium Salt ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Stem Segment ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
Shoot Initiation

Shoot tip and stem segment explants collected from greenhouse-maintained plants of Hymenoxys acaulis var. glabra were cultured in vitro for shoot initiation on a Murashige and Skoog (MS) medium supplemented with 30 g·L-1 sucrose, 2.5 μm BA, and 7 g·L-1 agar at a pH of 5.7. Unbranched shoot explants were subcultured to MS medium with 0.0, 0.5, 1, 2, 4 or 8 μm BA for shoot proliferation. A maximum of 10.3 shoots per explant was produced on the medium with 2.0 μm BA. Nonrooted shoots were subcultured to MS medium with 0.0, 0.5, 2, or 8 μm K-IBA for rooting. Maximum rooting was 90% on MS medium with 0.5 μm K-IBA. Rooted shoots were greenhouse-acclimatized for 10 days. Overall survival was 75%. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).

scholarly journals (175) In Vitro Regeneration of Cladrastis kentukea

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1052C-1052
Author(s):  
Denita Hadziabdic ◽  
Robert N. Trigiano ◽  
Stephen Garton ◽  
Mark T. Windham ◽  
William E. Klingeman
Keyword(s):  
Growth Regulators ◽  
Root Length ◽  
Woody Plant ◽  
In Vitro Regeneration ◽  
Cambial Activity ◽  
Ms Medium ◽  
Total Root Length ◽  
Woody Plant Medium ◽  
Half Strength

Axillary buds from a single Cladrastis kentukea tree were initially cultured on two media, woody plant medium (WPM) and Murashige and Skoog (MS) containing 0, 1, 2, or 4 μm 6–benzylaminopurine (BA). Cultures were transferred to fresh media every 4 weeks. Elongated shoots were harvested after 39 weeks and transferred to half-strength MS medium supplemented with the following concentrations of IBA: 0, 3, 30, 100, and 300 μm for 3 d, then returned to half-strength MS without growth regulators. Explants exposed to 300 μm of IBA produced significantly more roots (75%) compared to explants exposed to other treatments. Fifty-four and 45% of the microshoots rooted when exposed to 100 and 30 μm IBA, respectively. Only 4% of the microshoots rooted when exposed to 3 μm IBA and none of the control microshoots rooted. Although the 300 μm treatment yielded the most rooted plantlets, there was significantly higher terminal meristem abortion compared to other treatments. There were no statistical differences between the numbers of roots and total root length among all treatments. Additionally, all microshoots that rooted had lenticels, suggesting that presence of lenticel cambial activity can possibly improve rooting abilities of selected microshoots. Rooted microshoots were gradually acclimatized to nonsterile environment.

Period of harvest, sprouting ability of cuttings, and in vitro plant regeneration in Fraxinus excelsior

1994 ◽  
Vol 72 (2) ◽  
pp. 261-267 ◽  
Author(s):  
Conceição Eneida Silveira ◽  
Alain Cottignies
Keyword(s):  
Chromosome Number ◽  
In Vitro Culture ◽  
Adventitious Roots ◽  
Woody Plant ◽  
Fraxinus Excelsior ◽  
Stem Cuttings ◽  
Natural Conditions ◽  
Apical Bud ◽  
Woody Plant Medium

Propagation by stem cuttings and in vitro culture of apical bud explants were studied on Fraxinus excelsior L. Stem cuttings from 4- to 7-year-old trees growing under natural conditions sprouted only when cuttings were taken from dormant material. Only 6% of those that had sprouted developed roots by the 7th month of culture. Similarly, only apical bud explants harvested during the dormant period sprouted in vitro. Up to 87% of these sprouts developed two to four branching adventitious roots after 5 months of culture. During the initial phase of in vitro culture, the Quoirin and Lepoivre medium and the woody plant medium favoured sprout lengthening. During the phase of multiplication, up to three sprouts per explant developed with the woody plant medium in the presence of a combination of high 6-benzylaminopurine (3.0–4.0 mg∙L−1) and low indole-3-butyric acid (0.01–0.03 mg∙L−1) concentrations. Rooting was obtained in a medium without any growth regulators. Microscopic analysis showed a direct connection between the vascular elements of adventitious roots and stem of plantlet. Chromosome number in root apices of ash plantlets and ash trees grown under natural conditions was 2n = 46. Key words: chromosome number, Fraxinus excelsior L., in vitro plants, micropropagation, stem cuttings.

scholarly journals GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
JÚLIO CÉSAR GOMES PEREIRA ◽  
SELMA SILVA ROCHA ◽  
LUCIANA CARDOSO NOGUEIRA LONDE ◽  
MARCELA CAROLINE BATISTA DA MOTA ◽  
PABLO FERNANDO SANTOS ALVES ◽  
...  
Keyword(s):  
Culture Medium ◽  
Economic Importance ◽  
Leaf Length ◽  
Naphthalene Acetic Acid ◽  
Synthetic Seed ◽  
Ms Medium ◽  
World Production ◽  
Number Of Leaves ◽  
Alginate Matrix

ABSTRACT The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 µmol L-1) associated with NAA (naphthalene acetic acid) 0.54 µmol L-1, totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 µmol L-1 in the capsule composition are not indicated for microshoots growth.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals Impact of saline solution on growth and photosystem II during in vitro cultivation of Bromelia antiacantha (Bromeliaceae)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Rosiane Cipriano ◽  
João Paulo Rodrigues Martins ◽  
Luiz Carlos de Almeida Rodrigues ◽  
Antelmo Ralph Falqueto ◽  
Andreia Barcelos Passos Lima Gontijo
Keyword(s):  
Photosystem Ii ◽  
Saline Solution ◽  
Growth Traits ◽  
Photosynthetic Apparatus ◽  
Solution Culture ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Number Of Leaves ◽  
Positive Effect

Abstract In vitro cultivation is a technique with wide application for micropropagation. However, each species has specific mineral needs for this type of cultivation. The objective was to assess the impacts of the saline solution culture medium on the performance of the photosynthetic apparatus and growth of Bromelia antiacantha during in vitro cultivation, and thus to elucidate the mitigation of the nutritional imbalance that can interfere in the electron transport in the plants. Plants were cultivated in a salt concentration gradient of MS medium (0%, 25%, 50%, 75% or 100%). The growth traits and fluorescence a chlorophyll were analyzed. Intermediate concentrations of MS medium resulted in plants with a larger number of leaves and longer root length. The OJIP curves and results of the JIP test showed that the plants grown without MS salts presented less efficient photosystem II (PSII), as indicated by the performance index [Pi(total)]. In contrast, the intermediate concentrations (MS 25% and 50%) had a positive effect on the performance of the photosynthetic apparatus. The MS 25% medium can be used for in vitro cultivation of B. antiacantha, enabling the development of plants with suitable physiological qualities for planting in the field.

scholarly journals In vitro establishment and proliferation of red currant cultivars

2012 ◽  
Vol 39 (No. 1) ◽  
pp. 21-25 ◽  
Author(s):  
J. Sedlák ◽  
F. Paprštein
Keyword(s):  
Woody Plant ◽  
Adventitious Bud ◽  
Mineral Salts ◽  
The Czech Republic ◽  
Shoot Number ◽  
Adventitious Bud Formation ◽  
Woody Plant Medium ◽  
In Vitro Establishment ◽  
Red Currant

The goal of this study was to investigate in vitro multiplication protocols for use with red currant cultivars grown in the Czech Republic. Cultivars Detvan, Vitan and Rotte Höllandische were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. The overall rate of contamination was 25.7%. Two proliferation media Murashige and Skoog medium (MS) and McCown woody plant medium (WPM) containing 1 or 2 mg/l of 6-benzylaminopurine (BAP) were tested. Initial explants produced new plants in the form of rosettes. Rosettes arose from the base of the initial explants in the form of adventitious bud formation. The shoot number was relatively low and varied between 1.0 and 2.1. Generally, the highest number was obtained for cultivar Rotte Holländische that produced 2.1 ± 0.1 new rosettes on MS medium containing lower concentration 1 mg/l BAP. In contrary, Vitan cv. had significantly lower shoot number ranging from 1.0 to 1.3. WPM medium with a lower concentration of mineral salts proved to be unsuitable for the multiplication of tested cultivars.

scholarly journals In vitro growth and development of Dendrobium hybrid orchid

1970 ◽  
Vol 35 (3) ◽  
pp. 507-514 ◽  
Author(s):  
H Khatun ◽  
MM Khatun ◽  
MS Biswas ◽  
MR Kabir ◽  
M Al-Amin
Keyword(s):  
Growth Regulators ◽  
Growth And Development ◽  
Ms Medium ◽  
Fresh Weight ◽  
Maximum Weight ◽  
Shoot Height ◽  
Single Shoot ◽  
Number Of Leaves ◽  
Regenerated Plantlets

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination. Keywords: Dedrobium; orchid; hybrid; In vitro growth. DOI: 10.3329/bjar.v35i3.6457Bangladesh J. Agril. Res. 35(3) : 507-514

scholarly journals Optimization of Micropropagation Protocol for Goji Berry (Lycium barbarum L.)

2016 ◽  
Vol 73 (2) ◽  
pp. 141 ◽  
Author(s):  
Alexandru Fira ◽  
Nirmal Joshee ◽  
Victoria Cristea ◽  
Manuela Simu ◽  
Monica Harta ◽  
...  
Keyword(s):  
Shoot Proliferation ◽  
Optimal Number ◽  
Wheat Starch ◽  
Ms Medium ◽  
Ex Vitro ◽  
Benzyl Adenine ◽  
Lycium Barbarum ◽  
Floating Cell ◽  
The Media

Micropropagation of Lycium barbarum cv. 'Ningxia N1' was achieved. The cultures were by initiated by axenical seed germination. The highest shoot proliferation was obtained on the MS media with 1.33 or 2.22 µM benzyl adenine, gelled with wheat starch as an agar alternative. The treatments with 2.22 µM benzyl adenine ensured proliferation rates superior to the ones with 1.33 μM benzyl adenine, but the latter provided longer and more robust shoots. Use of large microcuttings as an explant onto the multiplication media ensured higher in vitro explant survival, higher number of shoots regeneration and more vigorous plantlets. The microcuttings inserted vertically into the media yielded superior growth and multiplication as compared to the microcuttings placed horizontally. The non-rooted, elongated shoots from the treatment 1.33 μM benzyl adenine were either rooted in vitro on a hormone-free MS medium with starch or used for direct ex vitro rooting and acclimatization. The optimal number of microcuttings/vessel for in vitro rooting was 40 and the rooted plantlets were efficiently acclimatized ex vitro by three methods: float hydroculture in floating cell trays, floating perlite, and in Jiffy7 pellets.

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