scholarly journals Effect of PRID-Delta devices associated with shortened estrus synchronization protocols on estrous response and fertility in dairy cows

2017 ◽  
Vol 17 (3) ◽  
pp. 757-770
Author(s):  
Irene López-Helguera ◽  
Fernando López-Gatius ◽  
Irina Garcia-Ispierto ◽  
Beatriz Serrano-Perez ◽  
Marcos G. Colazo
Keyword(s):  
Dairy Cows ◽  
Detection System ◽  
Estrus Synchronization ◽  
Not Given ◽  
Device Removal ◽  
New Device ◽  
Lactating Dairy Cows ◽  
Pregnancy Status ◽  
Intravaginal Device ◽  
Synchronization Protocol

Abstract This study evaluated the effect of a once-used progesterone (P4) intravaginal device (PRID) associated with four different shortened P4-based estrus synchronization (ES) protocols on estrous response (ER) and pregnancy per AI (P/AI) in cyclic and acyclic lactating dairy cows. Cows (n=465) were randomly assigned to one of the following protocols: 1) 2PGG, cows were given a PRID-Delta and 100 μg GnRH i.m. at PRID insertion (day 0). The PRID was left for 5 d, and 25 mg of dinoprost (PGF2α) i.m. given twice at PRID removal and 24 h later; 2) 2PGGe, same treatments as 2PGG plus 500 IU of eCG i.m. at PRID removal; 3) 2PGe, same treatments as 2PGGe, except GnRH was not given at PRID insertion; 4) PGe, same treatments as 2PGe, except PGF was only given at PRID removal. A total of 258 cows received a new PRID-Delta containing 1.55 g of P4, whereas 207 cows received a once-used PRID. Estrus was determined from P4 device removal until 96 h after using an automated heat detection system. Cows in estrus were given a second GnRH at AI and those without signs of estrus by 96 h after PRID removal were given GnRH and timed-AI (TA I). All inseminations were performed by one technician with commercially available frozen-thawed semen. Ultrasonography was performed at initiation of protocol and 28-34 days post AI to determine cyclicity and pregnancy status, respectively. Cows receiving once-used P4 devices had greater ER than cows receiving a new device (59.9 vs. 50.0; P=0.029), but P/AI did not differ between P4 devices, respectively (P>0.1; 40.6 vs 40.7%). Cyclic cows were less likely to display estrus than acyclic cows by a factor of 0.66 (P=0.036). Cows subjected to the 2PGe (2.41; P<0.01) protocol were more likely to display estrus than cows subjected to the 2PGG, whereas cows subjected to the PGe protocol did not differ (0.94; P=0.8) from those in the 2PGG group. Despite differences in ER, neither cyclicity nor estrus synchronization protocol affected P/AI (overall 40.6%). In summary, cyclic cows, those given a new P4 device and those subjected to either 2PGG or PGe protocol had reduced ER. However, all the factors examined had no significant effect on P/AI. All the estrus synchronization protocols resulted in acceptable fertility.

13 Pre-synchronization by Induction of a Dominant Follicle Using a Progesterone Device in a GnRH-Based-Ovulation Synchronization Protocol in Lactating Dairy Cows

2018 ◽  
Vol 30 (1) ◽  
pp. 146
Author(s):  
M. P. Bottino ◽  
L. A. C. L. DaSilva ◽  
L. M. S. Simoes ◽  
G. Santos ◽  
I. Y. H. Martinez ◽  
...  
Keyword(s):  
Sustained Release ◽  
Dairy Cows ◽  
Prostaglandin F2α ◽  
Follicular Development ◽  
Experimental Period ◽  
Ovulation Rate ◽  
Dominant Follicle ◽  
Lactating Dairy Cows ◽  
Intravaginal Device ◽  
Synchronization Protocol

The objective was to compare a pre-synchronization protocol by induction of a dominant follicle using a progesterone intravaginal device before an Ovsynch protocol (Presynch) with Double-Ovsynch in lactating dairy cows. Lactating Bos indicus × Bos taurus crossbred cows (n = 440) were randomly allocated to 1 of 2 treatments (all IM injections): (1) Double-Ov (n = 228): GnRH (Day –17), prostaglandin F2α (PGF2α) 7 days later (Day –10) and gonadotropin-releasing hormone (GnRH) 3 days later (Day –7) followed by an Ovsynch protocol 7 days later (GnRH on Day 0, PGF2α on Day 7, GnRH on Day 9); (2) Presynch (n = 212): insertion of a sustained release progesterone intravaginal device (Day –10), 10 days later (Day 0), an Ovsynch protocol was initiated with progesterone device withdrawal on Day 7. All cows were artificially inseminated 15 to 20 h after the second GnRH injection of the Ovsynch protocol and were pregnancy checked by ultrasonography 30 and 60 days later. On a subsample (n = 102), ultrasonography was performed on Days 0, 7, 9, and 24 of the experimental period. On another subsample (n = 42), blood samples for progesterone analysis were taken on Days 0, 7, and 24. There were no differences between pre-synchronization methods on synchronization parameters [presence of a follicle >12 mm on D0, Double-Ov 94.2% (49/52) and Presynch 92.0% (46/50); P = 0.66], follicular diameter on the 1st GnRH (Double-Ov 17.2 ± 0.7 mm and Presynch 18.6 ± 0.9 mm; P = 0.28), ovulation rate to the 1st GnRH [Double-Ov 86.3% (44/51) and Presynch 81.2% (39/48); P = 0.50], synchronization rate [Double-Ov 84.6% (44/52) and Presynch 86.0% (43/50); P = 0.84], follicular diameter on the 2nd GnRH (Double-Ov 17.5 ± 0.6 mm and Presynch 18.0 ± 0.5 mm; P = 0.48), ovulation rate to the 2nd GnRH [Double-Ov 90.9% (40/44) and Presynch 86.0% (37/43); P = 0.48] and CL diameter on Day 24 (Double-Ov 27.9 ± 0.7 mm and Presynch 29.4 ± 0.9 mm; P = 0.19). Corpus luteum presence on Day 0 was different (P = 0.03) between treatments [Double-Ov 57.7% (30/52) and Presynch 36.0% (18/50)]. Moreover, there was no difference (P = 0.85) between pregnancy rates on Day 30 [Double-Ov 39.0% (89/228) and Presynch 40.1% (85/212)] or Day 60 after AI [Double-Ov 34.8% (79/227) and Presynch 38.7% (82/212); P = 0.41] and gestational loss between 30 and 60 days after AI [Double-Ov 7.9% (7/88) and Presynch 3.5% (3/85); P = 0.13]. Proportion of cows with P4 <1 ng mL−1 on Day 0 was similar between treatments [Double-Ov 13.6% (3/22) v. Presynch 5.0% (1/20); P = 0.37]. Likewise, the proportion of cows with P4 >1 ng mL−1 on Day 7 [Double-Ov 77.3% (17/22) v. Presynch 95.0% (19/20); P = 0.14] and P4 concentration on Day 24 (Double-Ov 4.7 ± 0.6 v. P4-Ov 5.9 ± 0.9; P = 0.84) were similar between treatments. In conclusion, pre-synchronization by induction of follicular persistence using a sustained-release progesterone device before Ovsynch yielded similar results to the Double-Ovsynch protocol on follicular development and regression patterns and on the fertility of lactating dairy cows.

Effect of Herbal Galactogogue (Sanjivani biokseera) on Milk Yield and Milk Constituents in Lactating Kankrej Cattle at Organised Farm

2019 ◽  
Vol 15 (02) ◽  
pp. 39-41
Author(s):  
H H Panchasara ◽  
A B Chaudhari ◽  
D A Patel ◽  
Y M Gami ◽  
M P Patel
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Composition ◽  
Nutritional Requirements ◽  
Not Given ◽  
Distinct Effect ◽  
Lactating Dairy Cows ◽  
Group I ◽  
Effect Of Feeding

The study was conducted to evaluate the effect of feeding herbal galactogogue preparation (Sanjivani biokseera) on the milk yield and milk constituents in lactating Kankrej cows. Thirty-two lactating Kankrej cows in their 1st to 6th lactation were taken for the experiment from 3 days after calving up to 52 days postpartum. All the animals were fed as per the standard seasonally available roughages and concentrates to meet their nutritional requirements. The cows were randomly divided into two uniform groups of 16 cows in each according to initial milk yield and milk composition. The animals in group-I were not given any supplement and served as control. The animals in group-II were given Sanjivani biokseera (Naturewell Industries) @ 60 g per day for 1-month, commencing 3 days after calving, in addition to the usual feeds/fodders. A clear difference was observed in milk yield from day 8 onward of experiment between groups with significant (plessthan0 0.05) higher values from day 16-52 in cows fed herbal galactogogue as compared to control, but no such distinct effect on milk constituents was observed on day 52 when analyzed. The use of herbal galactogogue significantly (p lessthan 0.05) increased the overall average of 52 days milk production, which was 9.34 ± 0.21 lit/day in supplemented as compared to 7.75 ± 0.26 lit/day in control animals. It was concluded that herbal galactogogue (Sanjivani biokseera) could increase milk yield in lactating dairy cows through its galactopoetic property and improved rumen environment.

72 EFFECT OF PROGESTERONE SUPPLEMENTATION ON DAY 4 AFTER TIMED ARTIFICIAL INSEMINATION ON PREGNANCY RATE OF LACTATING DAIRY COWS

2017 ◽  
Vol 29 (1) ◽  
pp. 143
Author(s):  
G. Tortorelli ◽  
A. J. Azrak ◽  
V. da Costa Andrade ◽  
R. dos Santos Ramos ◽  
A. S. Moraes ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Fixed Time ◽  
Lactating Dairy Cows ◽  
Embryonic Loss ◽  
Progesterone Supplementation ◽  
Group 2 ◽  
Intravaginal Device ◽  
One Way Anova ◽  
Group 1

Progesterone plays an important role in the maintenance of pregnancy. It is hypothesised that insufficient progesterone early in pregnancy may result in embryonic loss, and that supplemental progesterone would decrease pregnancy loss in dairy cows. The aim of this study was to compare the effect of progesterone supplementation after timed AI on pregnancy rate of dairy cows. The experiment was conducted in a single commercial herd, in Sao Paulo State (Brazil), during a period of 1 year (January–December 2016). The cows were assigned at a nonpregnancy diagnosis 32 ± 3 days after AI to 1 to 6 resynchronization treatments. They were submitted to the same fixed time AI protocol: Day 0, 0.1 mg of gonadorelin, 2 mg of oestradiol benzoate, and an intravaginal device of 1.9 g of progesterone; Day 7, 25 mg of dinoprost tromethamine; Day 8, 25 mg of dinoprost tromethamine, 1 mg of oestradiol cipionate, and device withdrawal; Day 10, AI. At Day 14, the cows were alternately arranged in 2 groups. Group 1 received a 900-mg progesterone supplementation IM injection, and Group 2 did not receive progesterone supplementation. Then, 32 ± 3 days after insemination, pregnancy diagnosis was performed by transrectal ultrasonography, and the data were tabulated. Full statistical analysis will be done at the end of the study, when more than 500 cows will be allocated to the 2 groups. From January until April 2016, 230 lactating dairy cows were submitted to the experiment. Both groups had similar average days in lactation (166), average number of lactations (1.95), and average number of inseminations (4.3). Group 1 had a pregnancy rate of 25% (29/116), and group 2 had a pregnancy rate of 15.8% (18/114) (P = 0.01, one-way ANOVA). Based on these preliminary data, pregnancy rates of lactating dairy cows may be improved with progesterone supplementation on Day 4 after insemination. These results need to be confirmed on larger numbers of cows.

scholarly journals Saliva Crystallization in Sheep Subjected to Estrus Induction and Synchronization Protocols

2019 ◽  
Vol 48 ◽  
Author(s):  
Andressa Silveira Gonçalves ◽  
Ender Rosana Oberst ◽  
Raquel Fraga e Silva Raimondo
Keyword(s):  
Random Phase ◽  
Estrus Cycle ◽  
Device Removal ◽  
Financial Investment ◽  
Bodily Fluids ◽  
Implant Placement ◽  
Simple Alternative ◽  
The Subject ◽  
Intravaginal Device ◽  
Synchronization Protocol

Background: The crystallization of bodily fluids, primarily saliva, has been the subject of study in many species and is a simple alternative to detect estrus because it demands neither a significant financial investment nor qualified professionals to execute the examination. Fern pattern crystallization has been described in the cervical and nasal mucus, saliva and tear secretion, and in colostrum. Changes in salivary crystallization during the reproduction cycle are related to different hormonal concentrations in this period. Thus, the present study has evaluated the patterns of saliva crystallization in sheep subjected to estrus induction and synchronization protocols. Materials, Methods & Results: The sample consisted of 11 crossbreed Corriedale sheep, which were evaluated during two experimental periods (spring and autumn), and that underwent induction and synchronizing estrus protocols. In a random phase of the estrus cycle (day 0), each sheep was implanted with an intravaginal device (Primer®), impregnated with 0.36 g of progesterone for seven days. This device was inserted according to manufacturer’s instructions of the manufacturer and with the assistance of a specific applicator. On the day of device removal (day 7), the animals received 0.0375mg of D-Cloprostenol (Prolise®) and 10 mg of Folltropin extracted from the swine pituitary (NIH-FSH-P1 of Folltropin-V - Folltropin®) by intramuscular administration. The saliva was collected at six points during the experimental periods: day 1 (3 days before placement of the implant); day 4 (day of insertion of the implant); day 9 (5 days after the insertion of the implant); day 11 (day of removal of the implant and application of hormones); day 12 (24 h after removal of the implant [presumable estrus]); and day 13 (48 h after removal of the implant). Smears containing 10µ L of saliva were observed under an optical light microscope (200 x magnification) for assessment of saliva crystallization. Salivary crystallization was classified in scores from 0 to 3 based on the extent of crystallization observed in the slide, also considering filling of the observation field (x 200) and type of arborization pattern observed, as follows: 0 = without crystallization; 1 = too little crystallization; 2 = low crystallization; and 3 = large amount of crystallization. The patterns of crystallization found in sheep demonstrated an increase in quantity, diversity, definition, and size of sheets as the period of estrus approached and were different than those tipically described in the literature. Patterns of salivary crystallization followed the phases of the estrus cycle defined by the estrus induction and synchronization protocol.Discussion: Salivary crystallization in the sheep used in this study exhibited patterns that were different than those typically described in the literature. In the spring, salivary crystallization score demonstrated variation from day1 to 11 of the protocol, when the predominant hormone was progesterone released by the implant (diestrus). However, in Autumn, on day 4 (implant placement day), an increase in crystallization score was noted. This may have occurred because the sheep were in the reproductive season and therefore, may have been nearing seasonal estrus before placement of the implant. The patterns of crystallization found in sheep demonstrated an increase in quantity, diversity, definition, and size of sheets as the period of estrus approaches and showed patterns that were different than the ones usually described in literature. The patterns of salivary crystallization followed the phases of the estrus cycle defined by the estrus induction and synchronization protocol.

scholarly journals PSII-21 Vaginitis incidence and effects on fertility of beef females enrolled on estrus synchronization protocols using a controlled internal drug release (CIDR) device

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 242-242 ◽  
Author(s):  
Nicholas W Dias ◽  
Alvaro Sales ◽  
Claire Timlin ◽  
Stefania Pancini ◽  
John Currin ◽  
...  
Keyword(s):  
Drug Release ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Pregnancy Rates ◽  
Estrus Synchronization ◽  
Pregnancy Status ◽  
Timed Artificial Insemination ◽  
System 1 ◽  
Synchronization Protocol

Abstract Our objectives were to characterize the incidence of vaginitis caused by a controlled internal drug release (CIDR) device and its effects on fertility of beef females enrolled on estrus synchronization followed by timed-artificial insemination (TAI). A total of 1,097 mature cows and 215 heifers from 9 different locations were enrolled in the 7-d CO-Synch + CIDR synchronization protocol. At CIDR insertion, all females were inspected for any previous signs of vaginitis prior to enrollment in the experiment. As evidence of vaginitis, a CIDR score was determined at CIDR removal, and an AI plastic sheath score was also performed at TAI following a 1 to 4 system: 1=clean presenting no secretion; 2=clean presenting clear secretion; 3=presence of purulent secretion; 4=presence of purulent secretion and blood. Pregnancy status was determined by rectal ultrasonography approximately 40 days after TAI. The scores for CIDR and AI plastic sheath did not differ between cows and heifers. Of all females, 6% had a CIDR score of 1, 31% score 2, 56% score 3, and 7% score 4. Plastic sheath score was 1 for 16%, 2 for 62%, 3 for 15%, and 4 for 7% of females. Pregnancy rate to TAI differed between cows and heifers (P &lt; 0.01), among locations (P &lt; 0.001) and ranged from 36 to 66%; however, it was not influenced by CIDR score (P = 0.259) and plastic sheath score (P = 0.785). Pregnancy rate of females by different CIDR scores was 60%, 57%, 52%, and 46% for scores 1, 2, 3, and 4, respectively. Pregnancy rate of females by different AI plastic sheath score was 60%, 55%, 45%, and 48%, for scores 1, 2, 3, and 4, respectively. The presence of vaginitis caused by a CIDR does not influence pregnancy rates of TAI in beef females.

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