scholarly journals Utilization of unripe banana peel waste as feedstock for ethanol production

Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Ashish G. Waghmare ◽  
Shalini S. Arya
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Acid Hydrolysis ◽  
Chemical Characterization ◽  
Banana Peel ◽  
Ethanol Conversion ◽  
Fermentation Conditions ◽  
Acid Hydrolysate ◽  
Flame Ionization ◽  
Banana Peel Powder

AbstractBanana is second largest produced fruit of total world’s fruits. Cooking banana or plantains processing industry is generating enormous amount of waste in the form of unripe banana peel at one place, thus important to study waste management and utilization. Therefore, unripe banana peel was investigated for ethanol production. This study involved chemical characterization, optimization of acid hydrolysis, selection of yeast strain and optimization of fermentative production of ethanol from dried unripe banana peel powder (DUBPP). Ethanol concentration was determined using gas chromatography flame ionization detector (GC-FID). Characterization of DUBPP revealed notably amount of starch (41% w/w), cellulose (9.3% w/w) and protein (8.4% w/w). 49.2% w/w of reducing sugar was produced by acid hydrolysis of DUBPP at optimized conditions. Three yeast strains of Saccharomyces cerevisiae were screened for ethanol conversion efficiency, osmotolerance, ethanol tolerance, thermotolerance, fermentation ability at high temperature and sedimentation rate. Further, fermentation conditions were optimized for maximum ethanol production from acid hydrolysate of DUBPP. At optimized fermentation conditions, 35.5 g/l ethanol was produced using selected strain of Saccharomyces cerevisiae NCIM 3095. Hence, unripe banana peel waste can be good feedstock for ethanol production.

scholarly journals Ethanol Production from Nondetoxified Dilute-Acid Lignocellulosic Hydrolysate by Cocultures of Saccharomyces cerevisiae Y5 and Pichia stipitis CBS6054

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ping Wan ◽  
Dongmei Zhai ◽  
Zhen Wang ◽  
Xiushan Yang ◽  
Shen Tian
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Synthetic Medium ◽  
Pichia Stipitis ◽  
Dilute Acid ◽  
Acid Hydrolysate ◽  
Issatchenkia Orientalis ◽  
Final Ethanol Concentration

Saccharomyces cerevisiae Y5 (CGMCC no. 2660) and Issatchenkia orientalis Y4 (CGMCC no. 2159) were combined individually with Pichia stipitis CBS6054 to establish the cocultures of Y5 + CBS6054 and Y4 + CBS6054. The coculture Y5 + CBS6054 effectively metabolized furfural and HMF and converted xylose and glucose mixture to ethanol with ethanol concentration of 16.6 g/L and ethanol yield of 0.46 g ethanol/g sugar, corresponding to 91.2% of the maximal theoretical value in synthetic medium. Accordingly, the nondetoxified dilute-acid hydrolysate was used to produce ethanol by co-culture Y5 + CBS6054. The co-culture consumed glucose along with furfural and HMF completely in 12 h, and all xylose within 96 h, resulting in a final ethanol concentration of 27.4 g/L and ethanol yield of 0.43 g ethanol/g sugar, corresponding to 85.1% of the maximal theoretical value. The results indicated that the co-culture of Y5 + CBS6054 was a satisfying combination for ethanol production from non-detoxified dilute-acid lignocellulosic hydrolysates. This co-culture showed a promising prospect for industrial application.

A novel approach for the improvement of ethanol fermentation by Saccharomyces cerevisiae

2010 ◽  
Vol 56 (6) ◽  
pp. 495-500 ◽  
Author(s):  
Lihua Hou ◽  
Xiaohong Cao ◽  
Chunling Wang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Genome Engineering ◽  
Ethanol Conversion ◽  
Mitotic Chromosomes ◽  
Gene Encoding ◽  
Very High Gravity ◽  
Novel Approach ◽  
Very High Gravity Fermentation ◽  
Tetraploid Strain

Fermentation properties under the control of multiple genes are difficult to alter with traditional methods in Saccharomyces cerevisiae . Here, a novel genome engineering approach is developed to improve ethanol production in very high gravity fermentation with 300 g/L glucose as the carbon source. This strategy involved constructing aneuploid strains on the base of tetraploid cells. The tetraploid strain was constructed by using the plasmid YCplac33-GHK, which harbored the HO gene encoding the site-specific Ho endonucleases. The aneuploid strain, WT4-M, was selected and screened after the tetraploid cells were treated with methyl benzimidazole-2-yl-carbamate to induce loss of mitotic chromosomes. It was found that aneuploid strain WT4-M not only exhibited an increase in ethanol production and osmotic and thermal tolerance, but also an improvement in the sugar–ethanol conversion rate. Notably, WT4-M provided up to 9.8% improvement in ethanol production compared with the control strain. The results demonstrated that the strategy of aneuploidy was valuable for creating yeast strains with better fermentation characteristics.

EFFECTS OF FERMENTATION CONDITIONS ON ETHANOL PRODUCTION FROM CORN BY Saccharomyces cerevisiae MS42

2016 ◽  
Vol 61 (9) ◽  
pp. 169-176
Author(s):  
Nguyen Quang Thao ◽  
Nguyen Van Quyen ◽  
Nguyen Thao Anh ◽  
Nguyen Thanh Dat
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Fermentation Conditions

scholarly journals Effects of Ultrasound on Fermentation of Glucose to Ethanol by Saccharomyces cerevisiae

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 16 ◽  
Author(s):  
Luis Huezo ◽  
Ajay Shah ◽  
Frederick Michel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mass Transfer ◽  
Glucose Uptake ◽  
Ethanol Production ◽  
Ethanol Yield ◽  
Biofuel Production ◽  
Inhibitory Effects ◽  
Negative Effects ◽  
Intraparticle Mass Transfer ◽  
Improve Mass Transfer

Previous studies have shown that pretreatment of corn slurries using ultrasound improves starch release and ethanol yield during biofuel production. However, studies on its effects on the mass transfer of substrates and products during fermentation have shown that it can have both beneficial and inhibitory effects. In this study, the effects of ultrasound on mass transfer limitations during fermentation were examined. Calculation of the external and intraparticle observable moduli under a range of conditions indicate that no external or intraparticle mass transfer limitations should exist for the mass transfer of glucose, ethanol, or carbon dioxide. Fermentations of glucose to ethanol using Saccharomyces cerevisiae were conducted at different ultrasound intensities to examine its effects on glucose uptake, ethanol production, and yeast population and viability. Four treatments were compared: direct ultrasound at intensities of 23 and 32 W/L, indirect ultrasound (1.4 W/L), and no-ultrasound. Direct and indirect ultrasound had negative effects on yeast performance and viability, and reduced the rates of glucose uptake and ethanol production. These results indicate that ultrasound during fermentation, at the levels applied, is inhibitory and not expected to improve mass transfer limitations.

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