High genetic diversity and a new cryptic species within the Ephedrus persicae species group (Hymenoptera: Braconidae: Aphidiinae)

AFLP, RAPD and SSR molecular markers were used to study the genetic diversity and genetic structure of the Híbrido de Timor germplasm. The principal coordinate analysis, UPGMA cluster analysis based on genetic dissimilarity of Jaccard, Bayesian model-based cluster analysis, percentage of polymorphic loci, Shannon's information index and Nei gene diversity were employed to assess the genetic diversity. The analyses demonstrated a high genetic diversity among Híbrido de Timor accessions. UPGMA and Bayesian cluster analyses grouped the accessions into three clusters. The genetic structure of Híbrido de Timor is reported. The management of Híbrido de Timor germplasm variability and its potential use in breeding programs are discussed.

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Genetic diversity and molecular survey of Trypanosoma (Megatrypanum ) theileri in cattle in Brazil’s western Amazon region

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-296120180049 ◽

2018 ◽

Vol 27 (4) ◽

pp. 579-583 ◽

Cited By ~ 1

Author(s):

Thábata dos Anjos Pacheco ◽

Arlei Marcili ◽

Andrea Pereira da Costa ◽

Rute Witter ◽

Andréia Lima Tomé Melo ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Molecular Diagnosis ◽

Prevalence Rate ◽

Cathepsin L ◽

Ssu Rdna ◽

Amazon Region ◽

Prevalence Rates ◽

High Genetic Diversity ◽

First Time

Abstract Trypanosoma (Megatrypanum) theileri is a flagellated protozoan that infects ruminants and it displays high genetic diversity. In this study, we investigated the prevalence rates of this protozoan based on hemoculture and molecular diagnosis. The isolates of T. theileri thus obtained were characterized by molecular markers SSU rDNA and gGAPDH and molecular diagnosis based on Cathepsin L-like gene (PCR-TthCATL). The PCR-TthCATL and hemoculture indicated an overall prevalence rate of 8.13%, and the CATL derived sequence named IB was identified for the first time in cattle in the western Amazon region, as well as IF in Brazil. We also describe a possible new PCR-TthCATL derived sequence in cattle, designated IL.

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Short Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d201032 ◽

2019 ◽

Vol 20 (10) ◽

Author(s):

RINA SRI KASIAMDARI ◽

Ganies Riza Aristya ◽

HERI PRABOWO ◽

MUHAMMAD FAUZI ARIF ◽

Christy Ariesta

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Binary Data ◽

Crop Production ◽

Saccharum Officinarum ◽

High Genetic Diversity ◽

Sugarcane Plant ◽

Sugarcane Varieties ◽

Pair Group ◽

The Cost

Abstract. Kasiamdari RS, Aristya GR, Prabowo H, Ariesta C, Arif MF. 2019. Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers. Biodiversitas 20: 3005-3010. Sugarcane (Saccharum officinarum L.) belongs to the Poaceae family which are widely used for sweeteners production. In Indonesia, the need for sugarcane continues to increase along with its increased use in the production of bioethanol from molasses, a by-product of sugar production. However, the supply for sugar is still far from enough. Improvement of sugarcane productivity can be done by selecting superior varieties to form a new superior hybrid. Maintaining high genetic diversity in a population is essential. Selection of varieties is important because it can increase productivity without increasing the cost of crop production. Even the selected pest-resistant varieties can reduce the cost of crop production. Indonesia had produced many superior sugarcane plant varieties, but studies on their characters and genetic relationship are still lacking. Several molecular markers have been used to elucidate the genetic diversity among sugarcane varieties. Random Amplified Polymorphism DNA (RAPD) is one of the molecular markers that have been proven to describe genetic diversity among sugarcane plants. This study aimed to determine the genetic diversity among 22 superior sugarcane varieties in Indonesia based on eight RAPD molecular markers. The superior sugarcane varieties in Indonesia in this study were obtained from Indonesian Sweetener and Fiber Crops Research Institute (ISFRI), Malang and Madukismo Sugar Factory, Yogyakarta. DNA products were visualized by using gel electrophoresis and resolved bands were scored as binary data as presence (1) and absence (0). Linkage distance was done based on Jaccard’s similarity coefficient, and clustering analysis was performed based on the unweighted pair- group average (UPGMA) method. Polymorphism Information Content (PIC) analysis showed a high degree of polymorphism ranging from 0.76 to 0.92. Cluster analysis showed that 22 sugarcane varieties were divided into two main clusters and four sub-clusters based on their origin.

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Genetic diversity and morphological variability of fifteen Stachys (Lamiaceae) species from Iran using morphological and ISSR molecular markers

Biologia ◽

10.1515/biolog-2015-0051 ◽

2015 ◽

Vol 70 (4) ◽

Cited By ~ 1

Author(s):

Navaz Kharazian ◽

Somayeh Rahimi ◽

Behrouz Shiran

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Morphological Variability ◽

Natural Habitats ◽

High Genetic Diversity ◽

Morphological Variations ◽

Gene Variation ◽

Germplasm Diversity ◽

Issr Molecular Markers ◽

Zagros Region

AbstractStachys L. (Lamiaceae) exhibits a great range of variation with cosmopolitan distribution. An area extremely rich in taxa including 34 species has been introduced in Iran. This study documents the genetic diversity and morphological variations of 48 accessions from fifteen Stachys species and two subspecies belonging to six sections. All of the specimens were collected from natural habitats in Zagros region of Iran. In order to evaluate morphological variations, 21 characters were used. Eleven ISSR primers with DNA samples of leaves were applied to assess the genetic variations among species and populations of the Stachys genus. Based on the results, from the eleven primers screened, nine primers produced reproducible bands. The highest percentage of polymorphism included 100% in section Aucheriana. Analysis of molecular variance revealed a high proportion of gene variation (42%) among populations within species. In total sections, the UPGMA clustering dendrogram genetically detected different groups. Based on the morphological analysis, each section was definitely positioned. A high genetic diversity and polymorphism was found among Stachys accessions belonging to each section. Based on the findings, the germplasm diversity was due to the wealth genetic resources in the central Zagros region which are important in plant breeding and gene conservation purposes. In addition, morphological and ISSR molecular markers are useful to display the diversity in Stachys genus.

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Genetic relationships in Saccharum complex germplasm collections based on morphological and molecular markers

E-Journal Menara Perkebunan ◽

10.22302/iribb.jur.mp.v89i1.421 ◽

2021 ◽

Vol 89 (1) ◽

Author(s):

Wiwit Budi WIDYASARI ◽

. DAMANHURI

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Genetic Distance ◽

Genetic Relationship ◽

Genetic Similarity ◽

Genetic Relationships ◽

Morphological Markers ◽

Saccharum Complex ◽

High Genetic Diversity ◽

Germplasm Collections

and is a cross-pollinator, so information about the genetic relationship between the accessions in germplasm collections is very important for selecting the prospective parent in crossbreeding. This research aims to determine the phylogenetic relationship of 24 Saccharum complex accessions and to verify the grouping of accessions using 37 morphological and three microsatellite molecular markers. Interpretation of morphological and molecular data was obtained from the analysis using the NTYSYpc-2.02i program. The results show that within the 24 accessions analyzed using morphological markers, some accessions did not cluster as the classification at the conservation time. This difference is due to the morphological markers, descriptions of characters that do not appear are considered as the same variables though in different morphological characters, so this increases the value of genetic similarity among accessions analyzed. In contrast, the grouping of molecular markers shows that each accession was grouped according to the classification at the conservation time. These accessions had a low genetic similarity of 0.20 with a broad genetic distance of 0.80. This broad genetic distance indicates that the twenty-four accessions have a distant genetic relationship with one another, so that the genetic diversity of these accessions is relatively high. The high genetic diversity in germplasm collections improves its potential as a crossing parent to obtain a high heterosis effect.

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High genetic diversity within and low differentiation among Juniperus excelsa M. Bieb. populations: Molecular markers reveal their genetic structure patterns

TURKISH JOURNAL OF BOTANY ◽

10.3906/bot-2006-22 ◽

2021 ◽

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Genetic Structure ◽

High Genetic Diversity ◽

Juniperus Excelsa ◽

Structure Patterns

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Diversity of tortricid moths in apple orchards: evidence for a cryptic species ofGrapholita(Lepidoptera: Tortricidae) from China

Bulletin of Entomological Research ◽

10.1017/s0007485316000973 ◽

2016 ◽

Vol 107 (2) ◽

pp. 268-280 ◽

Cited By ~ 1

Author(s):

Y. Zheng ◽

R.X. Wu ◽

S. Dorn ◽

M.H. Chen

Keyword(s):

Genetic Diversity ◽

Cryptic Species ◽

Mitochondrial Gene ◽

Population Expansion ◽

Pheromone Trap ◽

Molecular Data ◽

Recent Population Expansion ◽

High Genetic Diversity ◽

First Report ◽

Specimen Collection

AbstractUnderstanding herbivore diversity both at the species and genetic levels is a key to effective pest management. We examined moth samples from multiple locations from a major apple growing region in China. For specimen collection, we used a pheromone trap designed to attractGrapholita molesta(Busck) (Lepidoptera: Tortricidae). Surprisingly, we found a second species captured at high proportions. Its external morphology (e.g., male genitalia and forewing coloration) was the same as forGrapholita funebranaTreitschke (Lepidoptera: Tortricidae) specimens from Europe. However, the barcode sequence of the mitochondrial gene cytochrome oxidase I (COI) diverged markedly between specimens from China and Europe, and the genetic distance value between the specimens from the two regions as estimated using the Juke-Cantor (JC) model amounted to 0.067. These morphological and molecular findings together point to a cryptic species inG. funebranafrom China. Further molecular analyses based on COI and COII genes revealed its extremely high genetic diversity, indicating that the origin of this species includes the sampling region. Moreover, molecular data suggest that this species passed through a recent population expansion. This is the first report on a cryptic species inG. funebrana, as well as the first report on its genetic diversity.

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Redescription of the last instar larva of Stenophylax fissus (McLachlan 1875) and the DNA barcode of Stenophylax crossotus McLachlan 1884 (Trichoptera: Limnephilidae) with an updated diagnostic matrix of the known larvae of Stenophylax species of the Iberian Peninsula

Zootaxa ◽

10.11646/zootaxa.4996.3.6 ◽

2021 ◽

Vol 4996 (3) ◽

pp. 513-524

Author(s):

ANTONIO RUIZ-GARCÍA ◽

ANDRÉS GARZÓN ◽

CARMEN ZAMORA-MUÑOZ

Keyword(s):

Genetic Diversity ◽

Iberian Peninsula ◽

Cryptic Species ◽

Dna Barcode ◽

Instar Larva ◽

High Genetic Diversity ◽

Mtcoi Gene

The larva of Stenophylax fissus (McLachlan 1875) is redescribed, illustrated, and compared with morphologically similar Limnephilidae larvae from the Iberian Peninsula. In addition, the sequence of the mtCOI gene in the barcode region of one individual is reported. Likewise, the barcode and the conspecificity between two larvae and one female of the non-sequenced species S. crossotus are given. In addition, the high genetic diversity among S. sequax populations is highlighted, suggesting a complex of cryptic species. Finally, a diagnostic matrix of the known larvae of Stenophylax species from the Iberian Peninsula is provided.

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Molecular phylogeny and species delimitation of the freshwater prawn Macrobrachium pilimanus species group, with descriptions of three new species from Thailand

PeerJ ◽

10.7717/peerj.10137 ◽

2020 ◽

Vol 8 ◽

pp. e10137

Author(s):

Warut Siriwut ◽

Ekgachai Jeratthitikul ◽

Somsak Panha ◽

Ratmanee Chanabun ◽

Chirasak Sutcharit

Keyword(s):

Genetic Diversity ◽

Species Delimitation ◽

Phylogenetic Analyses ◽

Species Group ◽

Freshwater Prawn ◽

Species List ◽

High Genetic Diversity ◽

Widespread Species ◽

Molecular Phylogenetic ◽

Montane Streams

Specific status and species boundaries of several freshwater prawns in the Macrobrachium pilimanus species group remain ambiguous, despite the taxonomic re-description of type materials and additional specimens collected to expand the boundaries of some species. In this study, the “pilimanus” species group of Macrobrachium sensu Johnson (1958) was studied using specimens collected from montane streams of Thailand. Molecular phylogenetic analyses based on sequences of three molecular markers (COI, 16S and 18S rRNA) were performed. The phylogenetic results agreed with morphological identifications, and indicated the presence of at least nine putative taxa. Of these, six morphospecies were recognised as M. malayanum, M. forcipatum, M. dienbienphuense, M. hirsutimanus, M. eriocheirum, and M. sirindhorn. Furthermore, three morphologically and genetically distinct linages were detected, and are described herein as M. naiyanetri Siriwut sp. nov., M. palmopilosum Siriwut sp. nov. and M. puberimanus Siriwut sp. nov. The taxonomic comparison indicated wide morphological variation in several species and suggested additional diagnostic characters that are suitable for use in species diagnoses, such as the shape and orientation of fingers, the rostrum form, and the presence or absence of velvet pubescence hairs and tuberculated spinulation on each telopodite of the second pereiopods. The “pilimanus” species group was portrayed as non-monophyletic in both ML and BI analyses. The genetic structure of different geographical populations in Thailand was detected in some widespread species. The species delimitation based on the four delimitation methods (BIN, ABGD, PTP and GMYC) suggested high genetic diversity of the “pilimanus” species group and placed the candidate members much higher than in previous designations based on traditional morphology. This finding suggests that further investigation of morphological and genetic diversity of Southeast Asian freshwater prawns in the genus Macrobrachium is still required to provide a comprehensive species list to guide efforts in conservation and resource management.

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