scholarly journals Genetic diversity and molecular survey of Trypanosoma (Megatrypanum ) theileri in cattle in Brazil’s western Amazon region

2018 ◽  
Vol 27 (4) ◽  
pp. 579-583 ◽  
Author(s):  
Thábata dos Anjos Pacheco ◽  
Arlei Marcili ◽  
Andrea Pereira da Costa ◽  
Rute Witter ◽  
Andréia Lima Tomé Melo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Molecular Diagnosis ◽  
Prevalence Rate ◽  
Cathepsin L ◽  
Ssu Rdna ◽  
Amazon Region ◽  
Prevalence Rates ◽  
High Genetic Diversity ◽  
First Time

Abstract Trypanosoma (Megatrypanum) theileri is a flagellated protozoan that infects ruminants and it displays high genetic diversity. In this study, we investigated the prevalence rates of this protozoan based on hemoculture and molecular diagnosis. The isolates of T. theileri thus obtained were characterized by molecular markers SSU rDNA and gGAPDH and molecular diagnosis based on Cathepsin L-like gene (PCR-TthCATL). The PCR-TthCATL and hemoculture indicated an overall prevalence rate of 8.13%, and the CATL derived sequence named IB was identified for the first time in cattle in the western Amazon region, as well as IF in Brazil. We also describe a possible new PCR-TthCATL derived sequence in cattle, designated IL.

Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 492f-493
Author(s):  
Roberto F. Vieira ◽  
James E. Simon ◽  
Peter Goldsbrough ◽  
Antonio Figueira
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Volatile Oil ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Traditional Medicines ◽  
The Tropics ◽  
High Degree ◽  
First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

scholarly journals Complete Genome Sequence of Psittacine Adenovirus 1, Identified from Poicephalus senegalus in Italy

2018 ◽  
Vol 7 (11) ◽  
Author(s):  
Adelaide Milani ◽  
Gianpiero Zamperin ◽  
Alice Fusaro ◽  
Annalisa Salviato ◽  
Luca Bano ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Full Genome Sequence ◽  
Full Genome ◽  
High Genetic Diversity ◽  
Content Type ◽  
First Time

Using a metagenomics approach, we were able to determine for the first time the full-genome sequence of a psittacine adenovirus 1 isolate that was recovered from the liver of a dead Senegal parrot (Poicephalus senegalus) in Italy. The results of the phylogenetic investigations revealed the existence of high genetic diversity among adenoviruses circulating in psittacine birds.

scholarly journals High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics

Viruses ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 385 ◽  
Author(s):  
Asimina Katsiani ◽  
Varvara Maliogka ◽  
Nikolaos Katis ◽  
Laurence Svanella-Dumas ◽  
Antonio Olmos ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Sour Cherry ◽  
Prunus Species ◽  
Cherry Tree ◽  
High Genetic Diversity ◽  
Viral Genomes ◽  
Virus Diversity ◽  
First Time

Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches. Our results reveal the presence of further genetic diversity within the LChV1 species. Interestingly, mixed infections of the same sweet cherry tree with different LChV1 variants were identified for the first time. Taken together, the high intra-host and intra-species diversities of LChV1 might affect its pathogenicity and have clear implications for its accurate diagnostics.

Non-monophyly of Retortamonadida and high genetic diversity of the genus Chilomastix suggested by analysis of SSU rDNA

2008 ◽  
Vol 48 (2) ◽  
pp. 770-775 ◽  
Author(s):  
Ivan Cepicka ◽  
Martin Kostka ◽  
Magdalena Uzlíková ◽  
Jaroslav Kulda ◽  
Jaroslav Flegr
Keyword(s):  
Genetic Diversity ◽  
Ssu Rdna ◽  
High Genetic Diversity

High genetic diversity and a new cryptic species within the Ephedrus persicae species group (Hymenoptera: Braconidae: Aphidiinae)

Biologia ◽  
2016 ◽  
Vol 71 (12) ◽  
Author(s):  
Andjeljko Petrović ◽  
Korana Kocić ◽  
Katarina Kos ◽  
Milan Plećaš ◽  
Vladimir Žikić ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Cryptic Species ◽  
Species Group ◽  
High Genetic Diversity

AbstractIn this study we used molecular markers to characterize various populations of the

scholarly journals Breeding potential and genetic diversity of "Híbrido do Timor" coffee evaluated by molecular markers

2010 ◽  
Vol 10 (4) ◽  
pp. 298-304 ◽  
Author(s):  
Tesfahun Alemu Setotaw ◽  
Eveline Teixeira Caixeta ◽  
Guilherme Ferreira Pena ◽  
Eunize Maciel Zambolim ◽  
Antonio Alves Pereira ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Molecular Markers ◽  
Genetic Structure ◽  
Gene Diversity ◽  
High Genetic Diversity ◽  
Breeding Programs ◽  
Information Index ◽  
Upgma Cluster Analysis ◽  
Potential Use

AFLP, RAPD and SSR molecular markers were used to study the genetic diversity and genetic structure of the Híbrido de Timor germplasm. The principal coordinate analysis, UPGMA cluster analysis based on genetic dissimilarity of Jaccard, Bayesian model-based cluster analysis, percentage of polymorphic loci, Shannon's information index and Nei gene diversity were employed to assess the genetic diversity. The analyses demonstrated a high genetic diversity among Híbrido de Timor accessions. UPGMA and Bayesian cluster analyses grouped the accessions into three clusters. The genetic structure of Híbrido de Timor is reported. The management of Híbrido de Timor germplasm variability and its potential use in breeding programs are discussed.

scholarly journals Short Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers

2019 ◽  
Vol 20 (10) ◽  
Author(s):  
RINA SRI KASIAMDARI ◽  
Ganies Riza Aristya ◽  
HERI PRABOWO ◽  
MUHAMMAD FAUZI ARIF ◽  
Christy Ariesta
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Binary Data ◽  
Crop Production ◽  
Saccharum Officinarum ◽  
High Genetic Diversity ◽  
Sugarcane Plant ◽  
Sugarcane Varieties ◽  
Pair Group ◽  
The Cost

Abstract. Kasiamdari RS, Aristya GR, Prabowo H, Ariesta C, Arif MF. 2019. Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers. Biodiversitas 20: 3005-3010. Sugarcane (Saccharum officinarum L.) belongs to the Poaceae family which are widely used for sweeteners production. In Indonesia, the need for sugarcane continues to increase along with its increased use in the production of bioethanol from molasses, a by-product of sugar production. However, the supply for sugar is still far from enough. Improvement of sugarcane productivity can be done by selecting superior varieties to form a new superior hybrid. Maintaining high genetic diversity in a population is essential. Selection of varieties is important because it can increase productivity without increasing the cost of crop production. Even the selected pest-resistant varieties can reduce the cost of crop production. Indonesia had produced many superior sugarcane plant varieties, but studies on their characters and genetic relationship are still lacking. Several molecular markers have been used to elucidate the genetic diversity among sugarcane varieties. Random Amplified Polymorphism DNA (RAPD) is one of the molecular markers that have been proven to describe genetic diversity among sugarcane plants. This study aimed to determine the genetic diversity among 22 superior sugarcane varieties in Indonesia based on eight RAPD molecular markers. The superior sugarcane varieties in Indonesia in this study were obtained from Indonesian Sweetener and Fiber Crops Research Institute (ISFRI), Malang and Madukismo Sugar Factory, Yogyakarta. DNA products were visualized by using gel electrophoresis and resolved bands were scored as binary data as presence (1) and absence (0). Linkage distance was done based on Jaccard’s similarity coefficient, and clustering analysis was performed based on the unweighted pair- group average (UPGMA) method. Polymorphism Information Content (PIC) analysis showed a high degree of polymorphism ranging from 0.76 to 0.92. Cluster analysis showed that 22 sugarcane varieties were divided into two main clusters and four sub-clusters based on their origin.     

Genetic diversity and morphological variability of fifteen Stachys (Lamiaceae) species from Iran using morphological and ISSR molecular markers

Biologia ◽  
2015 ◽  
Vol 70 (4) ◽  
Author(s):  
Navaz Kharazian ◽  
Somayeh Rahimi ◽  
Behrouz Shiran
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Morphological Variability ◽  
Natural Habitats ◽  
High Genetic Diversity ◽  
Morphological Variations ◽  
Gene Variation ◽  
Germplasm Diversity ◽  
Issr Molecular Markers ◽  
Zagros Region

AbstractStachys L. (Lamiaceae) exhibits a great range of variation with cosmopolitan distribution. An area extremely rich in taxa including 34 species has been introduced in Iran. This study documents the genetic diversity and morphological variations of 48 accessions from fifteen Stachys species and two subspecies belonging to six sections. All of the specimens were collected from natural habitats in Zagros region of Iran. In order to evaluate morphological variations, 21 characters were used. Eleven ISSR primers with DNA samples of leaves were applied to assess the genetic variations among species and populations of the Stachys genus. Based on the results, from the eleven primers screened, nine primers produced reproducible bands. The highest percentage of polymorphism included 100% in section Aucheriana. Analysis of molecular variance revealed a high proportion of gene variation (42%) among populations within species. In total sections, the UPGMA clustering dendrogram genetically detected different groups. Based on the morphological analysis, each section was definitely positioned. A high genetic diversity and polymorphism was found among Stachys accessions belonging to each section. Based on the findings, the germplasm diversity was due to the wealth genetic resources in the central Zagros region which are important in plant breeding and gene conservation purposes. In addition, morphological and ISSR molecular markers are useful to display the diversity in Stachys genus.

scholarly journals Combining Microsatellite Markers and Ampelography for Better Management of Romanian Grapevine Germplasm Collections

2018 ◽  
Vol 10 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Carmen Florentina POPESCU ◽  
Manna CRESPAN
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Phenotypic Variability ◽  
Molecular Analyses ◽  
Germplasm Collections ◽  
New Synonyms ◽  
The Moment ◽  
Genetic Profiles ◽  
First Time ◽  
The Eu

Aiming to investigate the correspondence of phenotypic variability and molecular markers, 50 varieties of Vitis vinifera ssp. vinifera, ancient grapevine varieties grown on Romanian territory, were selected to be analysed. All varieties were subjected to ampelographic analyses with OIV descriptors and also to molecular analyses with 13 microsatellites. The morphology description with recommended methods corresponded with the reference literature, proving the correct registration of the varieties at the moment of acquisition. Markers efficiency for assessing the genetic diversity among studied varieties was evaluated by computation of statistic parameters referring to the polymorphisms found. Among the internationally recommended SSR markers, ISV4 and VMCNG4b9 markers displayed interesting PIC and PI values. By comparing the SSR profiles of the 50 Romanian grapevine varieties with those in the Eu database, the following can be concluded: confirmation of 10 synonymies mentioned in old documents, while 3 synonymies mentioned in old reference literature were not confirmed, new synonyms were found for 4 accessions, additional synonyms were found for 4 accessions, which are to be added to the previous ones already mentioned in the literature, the synonymies in three groups of accessions were documented for the first time in  the specialised literature, the unicity of three Romanian accessions was proved (ʻMoroştinăʼ, ʻNegru mareʼ and ʻRomânieʼ) and the genetic profiles of 15 varieties were identical to those already recorded in the investigated databases

scholarly journals Genetic diversity of the Hungarian Gidran horse in two mitochondrial DNA markers

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1894 ◽  
Author(s):  
Nikolett Sziszkosz ◽  
Sándor Mihók ◽  
András Jávor ◽  
Szilvia Kusza
Keyword(s):  
Genetic Diversity ◽  
Genetic Characterization ◽  
Horse Breed ◽  
High Genetic Diversity ◽  
Mitochondrial Markers ◽  
Blast Analysis ◽  
D Loop ◽  
First Time ◽  
Mtdna Markers

The Gidran is a native Hungarian horse breed that has approached extinction several times. Phylogenetic analysis of two mitochondrial markers (D-loop and cytochrome-b) was performed to determine the genetic characterization of the Gidran for the first time as well as to detect errors in the management of the Gidran stud book. Sequencing of 686 bp ofCYTBand 202 bp of the D-loop in 260 mares revealed 24 and 32 haplotypes, respectively, among 31 mare families. BLAST analysis revealed six novelCYTBand four D-loop haplotypes that have not been previously reported. The Gidran mares showed high haplotype (CYTB: 0.8735 ± 0.011; D-loop: 0.9136 ± 0.008) and moderate nucleotide (CYTB: 0.00472 ± 0.00017; D-loop: 0.02091 ± 0.00068) diversity. Of the 31 Gidran mare families, only 15CYTB(48.4%) and 17 D-loop (54.8%) distinct haplotypes were formed using the two markers separately. Merged markers created 24 (77.4%) mare families, which were in agreement with the mare families in the stud book. Our key finding was that the Gidran breed still possesses high genetic diversity despite its history. The obtained haplotypes are mostly consistent with known mare families, particularly when the two mtDNA markers were merged. Our results could facilitate conservation efforts for preserving the genetic diversity of the Gidran.

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