scholarly journals Species delimitation and genetic diversity analysis in Salvia with the use of ISSR molecular markers

2016 ◽  
Vol 75 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Masoumeh Safaei ◽  
Masoud Sheidai ◽  
Behnaz Alijanpoor ◽  
Zahra Noormohammadi
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Species Delimitation ◽  
Morphological Characters ◽  
Mantel Test ◽  
Species Relationship ◽  
Inter Simple Sequence Repeats ◽  
Alborz Mountain ◽  
High Degree ◽  
Issr Molecular Markers

AbstractThirty-nine plant specimens of six Salvia species were collected from different localities of the Alborz mountain region in Iran and studied for morphological and genetic variability and species relationship. Inter simple sequence repeats (ISSR) molecular markers showed a high degree of within-species and interspecific genetic variability in Salvia. Analysis of molecular variance and Hickory tests showed significant molecular difference among the studied populations. A principal coordinate analysis plot of morphological characters grouped the species into two distinct groups, supporting their taxonomic treatment. This was partly supported by ISSR networking. The Mantel test did not show a correlation between genetic distance and the geographical distance of the studied populations. STRUCTURE and reticulation analyses revealed some degree of gene fl ow among the species. The present study showed that ISSR molecular markers could be used in Salvia species delimitation along with morphological study.

scholarly journals Species delimitation and relationship in Crocus L. (Iridaceae)

2018 ◽  
Vol 77 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Masoud Sheidai ◽  
Melica Tabasi ◽  
Mohammad-Reza Mehrabian ◽  
Fahimeh Koohdar ◽  
Somayeh Ghasemzadeh-Baraki ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Species Delimitation ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Specific Gene ◽  
Data Sets ◽  
Species Relationship ◽  
Nj Tree ◽  
High Degree ◽  
Issr Molecular Markers

AbstractThe genusCrocusL. (Iridaceae) is monophyletic and contains about 100 species throughout the world.Crocusspecies have horticultural, medicinal and pharmacological importance. Saffron is the dried styles ofC. sativusand is one of the world’s most expensive spices by weight. Controversy exits about the taxonomy of the genus and the species relationship. Exploring genetic diversity and inter-specific cross-ability are important tasks for conservation of wild taxa and for breeding of cultivatedC. sativus. The present study was performed to study genetic variability and population structure in fiveCrocusL. species includingCrocus almehensisBrickell & Mathew,C. caspiusFischer & Meyer,C. speciosusMarschall von Biberstein,C. haussknechtiiBoissier, andC. sativusL. by inter simple sequence repeat (ISSR) molecular markers. We also used published internal transcribed spacer (ITS) sequences to study species relationship and compare the results with ISSR data. The results revealed a high degree of genetic variability both within and among the studied species. Neighbor joining (NJ) tree and network analysis revealed that ISSR markers are useful inCrocusspecies delimitation. Population fragmentation occurred inC. caspiusandC. sativus. Both ISSR and sequenced based analyses separatedC. sativusfrom the other studied species. Close genetic affinity ofC. sativusandC. pallisiiand inter-specific gene flow was supported by both data sets.

Species delimitation and population structure in three Onosma (Boraginaceae) species

Biologia ◽  
2015 ◽  
Vol 70 (10) ◽  
Author(s):  
Masoud Sheidai ◽  
Samira Sadeghi ◽  
Mahnaz-Arab Ameri ◽  
Ahmad-Reza Mehrabeian
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Species Delimitation ◽  
Genetic Difference ◽  
Morphological Variability ◽  
Morphological Diversity ◽  
Mantel Test ◽  
Geographical Distance ◽  
Nj Tree ◽  
Issr Molecular Markers

AbstractThe present study aimed to reveal genetic diversity and population structure in three Onosma species i.e., Onosma bulbotrichum DC., O. elwendicum Wettst. and O. sericeum Willd. (Boraginaceae) growing in Iran. This study was performed to check if the ISSR molecular markers could be used in the Onosma species delimitation. We also investigated the infra-specific morphological variability and if we can identify infra-species taxonomic forms. The present study revealed within species genetic and morphological diversity. ISSR data could delimit the studied species as they were separated from each other in NJ tree, maximum parsimony and STRUCTURE analysis. AMOVA and Hickory tests produced significant genetic difference among the studied species. The Mantel test showed no correlation between genetic distance and geographical distance of the populations for all three species. Reticulation analysis and Nm estimation showed gene flow among these populations. We could identify a few ISSR loci that are adaptive. Data obtained can be used in conservation of these medicinal plants in Iran.

scholarly journals In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

2017 ◽  
Vol 45 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Constantinos SALIS ◽  
Ioannis E. PAPADAKIS ◽  
Spyridon KINTZIOS ◽  
Marianna HAGIDIMITRIOU
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Root Length ◽  
In Vitro Propagation ◽  
Genetic Relationships ◽  
Shoot Proliferation ◽  
Poncirus Trifoliata ◽  
Naphthaleneacetic Acid ◽  
Issr Molecular Markers

The behavior of six citrus rootstocks, Volkameriana, Citrumelo ‘Swingle’, Citrange ‘Carrizo’, Poncirus trifoliata ‘Serra’, Poncirus trifoliata ‘Rubidoux’ and Poncirus trifoliata ‘Flying Dragon’, in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock ‘Swingle’ showed a higher number of roots per explant (4.2) followed by ‘Flying Dragon’ (3.93) and ‘Carrizo’ (3.23) rootstocks. The rootstocks ‘Swingle’ (140.8 mm), Volkameriana (148 mm) and ‘Flying Dragon’ (131.12 mm) had significantly higher root length per explant compared to ‘Carrizo’ (31 mm) and ‘Rubidoux’ (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata ‘Serra’ and Citrumelo ‘Swingle’) and 0.60 (Volkameriana and Citrumelo ‘Swingle’). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.

scholarly journals Genetic diversity in Jatropha species from different regions of Brazil based on morphological characters and inters-simple sequence repeat (ISSR) molecular markers

2015 ◽  
Vol 14 (25) ◽  
pp. 2066-2079 ◽  
Author(s):  
Silva Rosa Pazeto Mariana ◽  
Helena Uneda Trevisoli Sandra ◽  
Arcenio Pimentel Correa Aretha ◽  
Formice Vianna Viviane ◽  
Carvalho Leite Daniel ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeat ◽  
Morphological Characters ◽  
Sequence Repeat ◽  
Issr Molecular Markers ◽  
Simple Sequence

scholarly journals RAPD and ISSR molecular markers in Olea europaea L.: Genetic variability and molecular cultivar identification

2006 ◽  
Vol 54 (1) ◽  
pp. 117-128 ◽  
Author(s):  
Paula Martins-Lopes ◽  
José Lima-Brito ◽  
Sónia Gomes ◽  
Julieta Meirinhos ◽  
Luís Santos ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Olea Europaea ◽  
Cultivar Identification ◽  
Olea Europaea L ◽  
Issr Molecular Markers ◽  
Rapd And Issr ◽  
Olea Europaéa

Species Delimitation In Rhabdosciadium (Apiaceae): Morphological and Molecular

Phytotaxa ◽  
2020 ◽  
Vol 437 (1) ◽  
pp. 1-13
Author(s):  
SHAHRAM MEHRI ◽  
IMAN KOLBADI ◽  
HASSAN SHIRAFKAN-AJIRLOU
Keyword(s):  
Species Delimitation ◽  
Morphological Diversity ◽  
Molecular Data ◽  
Mantel Test ◽  
Species Structure ◽  
Genetic Affinity ◽  
Data Set ◽  
Geographical Populations ◽  
Morphological And Molecular Data ◽  
Issr Molecular Markers

Rhabdosciadium is a distinct and small Apiaceae genus represented by only 8 species worldwide, 5 of which are distributed in Turkey and 3 occur in Iran. They all are narrow endemic, rare mountain species. The genus is characterized by single-fruited umbellules, linear-cylindrical fruits, parennial, glabrous, and junciform habit, reduced cauline leaves, and fibrous collar at the base of the stem. Till present time, there has been no detailed information available on molecular phylogeny and genetic structure of these species in the country. Therefore, the present study was conducted with the aim to investigate species delimitation by both morphological and molecular data and to reveal genetic diversity and population structure in these three Rhabdosciadium species. For this study, 98 randomly collected plants from 15 geographical populations in Rhabdosciadium species were used. We encountered extensive within species genetic and morphological diversity. ISSR molecular markers could delimit the studied species. STRUCTURE analysis revealed the occurrence gene flow between these species. The Mantel test showed correlation between genetic distance and geographical distance of the populations studied. Phylogenetic tree was constructed based on ITS data set which separated out-groups from the studied species. Genetic affinity of the studied species have been discussed.

Interpopulation Congruence in Chinese Primula ovalifolia Revealed by Chemical and Molecular Markers Using Essential Oils and ISSRs

2003 ◽  
Vol 58 (1-2) ◽  
pp. 57-61 ◽  
Author(s):  
Peng Nan ◽  
Shaolin Peng ◽  
Suhua Shi ◽  
Hai Ren ◽  
Ji Yang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Essential Oil ◽  
Essential Oils ◽  
Natural Populations ◽  
Issr Markers ◽  
Mantel Test ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

The chemical composition of the essential oils of five natural populations of P. ovalifolia from central and southwest China and their interpopulation variability were first analyzed by using GC-MS. Twenty-two essential oil compounds were obtained, in which eighteen ones were identified and characterized representing 95%-96% of the oil composition. Three main chemotypes, i. e., the methyl-acetyl-hydroquinone-rich, hydroquinone-rich, and acetyl-hydroquinone-rich chemotypes, were then differentiated, corresponding to the three groups obtained from the cluster analysis based on the essential oil composition percentages. Genetic variations among the five populations were also investigated using the Inter-Simple Sequence Repeats (ISSR) markers. Finally, the Mantel test showed that there was a significant correlation between two distance matrices based on the chemical compounds of essential oils and ISSR markers, confirming the congruence of interpopulation relationships in the P. ovalifolia revealed by the chemical and molecular markers

scholarly journals Pre-breeding studies in Panicum coloratum var. coloratum: Characterization using agro-morphological traits and molecular markers

2018 ◽  
Vol 6 (2) ◽  
pp. 82-92 ◽  
Author(s):  
Estanislao Burgos ◽  
Carolina Thompson ◽  
Mabel Giordano ◽  
Maria A. Thomas
Keyword(s):  
Molecular Markers ◽  
Seed Production ◽  
Morphological Characters ◽  
Forage Production ◽  
Complementary Information ◽  
Phenotypic Information ◽  
C4 Grass ◽  
Panicum Coloratum ◽  
Combined Use ◽  
Issr Molecular Markers

Panicum coloratum var. coloratum is a native African perennial C4 grass, introduced to Argentina. It is tolerant of salinity and cold and has good forage production. The scarce genotypic and phenotypic information about this grass limits its breeding in order to satisfy market demands. The aim of this study was to evaluate the variability in a collection of Panicum coloratum var. coloratum formed by 8 accessions and grown at EEA INTA Rafaela during the summer of 2011, based on 15 ISSR molecular markers and 17 morphological characters. For all morphological characters, the distribution of variability observed in the collection was high and not homogenous. The characters that showed greater variation were related to forage and seed production. Eight ISSRs, selected according to their reproducibility, showed 127 bands with 100% polymorphism and allowed grouping of populations according to their site of collection. AMOVA study indicated that more than 58% of the molecular variation existed within accessions; this would be consistent with the predominant allogamous form of reproduction. The results showed that the combined use of molecular and morphological markers offer complementary information. The high variability detected in this collection will allow for the initiation of a breeding program to improve important characters like those related to DM yield and seed production.

scholarly journals Genetic diversity and morphological variability in Geranium purpureum Vill. (Geraniaceae) of Iran

Genetika ◽  
2017 ◽  
Vol 49 (2) ◽  
pp. 543-557 ◽  
Author(s):  
Somayeh Bozchaloyi ◽  
Masoud Sheidai ◽  
Maryam Keshavarzi ◽  
Zahra Noormohammadi
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Morphological Variability ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Molecular Data ◽  
Mantel Test ◽  
Geographical Populations ◽  
Well Differentiated ◽  
Genetic Stratification

Genetic variability and populations, structure were studied in 15 geographical populations of Geranium purpureum Vill. (Geraniaceae). Genetic diversity parameters were determined in these populations. AMOVA and Gst analyses revealed the presence of genetic variability within populations and significant molecular difference among the studied populations. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. STRUCTURE and K-Means clustering revealed populations, genetic stratification. UPGMA dendrogram of populations based on morphological characters was in agreement with NJ tree of molecular data. These results indicated that geographical populations of G. purpureum are well differentiated both in genetic content as well as morphological characteristics. Consensus tree based on morphological and genetic data separated some of these populations from the others suggesting the existence of ecotypes within this species.

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