scholarly journals Comparison of hydrogen production in microalgae under autotrophic and mixotrophic media

2017 ◽  
Vol 23 (2) ◽  
pp. 169-177 ◽  
Author(s):  
Kritsana Duangjan ◽  
Watsamon Nakkhunthod ◽  
Jeeraporn Pekkoh ◽  
Chayakorn Pumas

AbstractHydrogen is an alternative source of energy of considerable interest, because it is environmentally friendly. Biological hydrogen production processes involving green microalgae are of significant interest. However, until present only few microalgae genera have been studied and almost all of those studies have focused only on cultivation using mixotrophic or heterotrophic media, which are expensive, and can be easily contaminated. This study aimed to compare the potential of biohydrogen production from novel green microalgae under autotrophic and mixotrophic media. A total of ninety strains of six orders of green microalgae were investigated for their capabilities of hydrogen production. The results showed that eleven novel hydrogen-producing microalgae genera were found. The hydrogen production in each order was influenced by the medium. Moreover, several strains presented notable levels of autotrophic hydrogen production and performed at over twice of the mixotrophic medium. These results should be supportive information for the selection and cultivation of hydrogen-producing microalgae in further studies.

2018 ◽  
Vol 20 (3) ◽  
pp. 564-571

<p>This study investigates the usability of sewage sludge, waste from a waste water treatment facility, at the stable thermophilic temperature and different pH conditions in the biohydrogen production by dark fermentation. Without the addition of a pure hydrogen producer and nutrient source, the effect of a different constant pH in the range of pH 4-9 on biohydrogen production using sewage sludge was compared with that of a different initial pH. It was understood from the results that biohydrogen production varies according to the characterization of sewage sludge. In the experiments, the lag time was insignificant (~2h). The maximum hydrogen production was achieved at pH 5 within the first 24-30 hours of fermentation (92894 mL m-3 H2). Therefore, it was determined that the higher digestion efficiencies of the sewage sludge were obtained at pH 5. In general, with the increase in methanogens in the medium, the hydrogen producing ability and hydrogen content of the sewage sludge gradually decreased. Hydrogen production at almost all the pH values after the third day was less than 1000 mL m-3.</p>


RSC Advances ◽  
2018 ◽  
Vol 8 (41) ◽  
pp. 22924-22930 ◽  
Author(s):  
Tao Sheng ◽  
Lei Zhao ◽  
Lingfang Gao ◽  
Wenzong Liu ◽  
Guofeng Wu ◽  
...  

An edible fungal pretreatment of rice straw was proposed for enhanced hydrogen production while reducing the chemical cost for traditional biological hydrogen production from lignocellulose.


Author(s):  
Wenfa Ng

Hydrogen is useful as a fuel and could be produced by a variety of means. One approach uses artificial photosynthesis where energy from sunlight powers the splitting of water into hydrogen and oxygen. But, biological methods for producing hydrogen has emerged strongly over the past decades. In particular, specific microorganisms could use different substrates to produce hydrogen at differing yields. Such fundamental discoveries with industrial applications thus motivated the use of metabolic engineering approaches and methodologies in enhancing biological hydrogen production through a series of enzyme over-expression, pathway debottlenecking, and gene deletion. However, such approaches heavily rely on the selection of an appropriate microbial chassis for biohydrogen production. With the proper strain in hand, use of alternative substrates may engender greater hydrogen productivities. But learning from the bioprocessing field, co-culture of two compatible microorganisms have been sought after for improving biohydrogen production. In addition, thermophilic microbes may also be useful candidates for exploiting hydrogen production from composting. Future outlook in the field looks into filling our gaps in understanding of the metabolic network that feeds into hydrogen production in different organisms. But, more importantly, problems such as reduced growth rate in engineered microbes point to fundamental issues with using genetically engineered microorganisms for improved biohydrogen production, to which clever bioprocess engineering may yield solutions.


Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1353 ◽  
Author(s):  
Neda Fakhimi ◽  
David Gonzalez-Ballester ◽  
Emilio Fernández ◽  
Aurora Galván ◽  
Alexandra Dubini

Biological hydrogen production by microalgae is a potential sustainable, renewable and clean source of energy. However, many barriers limiting photohydrogen production in these microorganisms remain unsolved. In order to explore this potential and make biohydrogen industrially affordable, the unicellular microalga Chlamydomonas reinhardtii is used as a model system to solve barriers and identify new approaches that can improve hydrogen production. Recently, Chlamydomonas–bacteria consortia have opened a new window to improve biohydrogen production. In this study, we review the different consortia that have been successfully employed and analyze the factors that could be behind the improved H2 production.


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