Rapid detection of non-deletional mutations causing α-thalassemia by multicolor melting curve analysis

2016 ◽  
Vol 54 (3) ◽  
Author(s):  
Qiuying Huang ◽  
Xudong Wang ◽  
Ning Tang ◽  
Chunjiang Zhu ◽  
Tizhen Yan ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Sanger Sequencing ◽  
Melting Curve ◽  
High Sensitivity ◽  
Cost Effective ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Sensitivity Specificity

AbstractThe assay consisted of one pair of primers specific for theThe assay had a reproducibility of 100%, could detect gDNA of different genotype as low as 1 ng per reaction, and had an overall accuracy of 100% when compared with RDB analysis and Sanger sequencing.The developed assay is rapid, robust, and cost-effective while maintaining high sensitivity, specificity, and throughput.

scholarly journals Cost-effective and fast KIR gene-content genotyping by multiplex melting curve analysis

HLA ◽  
2018 ◽  
Vol 92 (6) ◽  
pp. 384-391
Author(s):  
Leonardo M. Amorim ◽  
Tiago H. S. Santos ◽  
Jill A. Hollenbach ◽  
Paul J. Norman ◽  
Wesley M. Marin ◽  
...  
Keyword(s):  
Melting Curve ◽  
Cost Effective ◽  
Curve Analysis ◽  
Gene Content ◽  
Melting Curve Analysis ◽  
Kir Gene

scholarly journals Melting Curve Analysis for Rapid Detection of Topoisomerase Gene Mutations in Haemophilus influenzae

2009 ◽  
Vol 47 (3) ◽  
pp. 781-784 ◽  
Author(s):  
S. Nakamura ◽  
K. Yanagihara ◽  
Y. Morinaga ◽  
K. Izumikawa ◽  
M. Seki ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Rapid Detection ◽  
Melting Curve ◽  
Gene Mutations ◽  
Curve Analysis ◽  
Melting Curve Analysis
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