scholarly journals Non-invasive determination of uric acid in human saliva in the diagnosis of serious disorders

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Andrea Vernerová ◽  
Lenka Kujovská Krčmová ◽  
Bohuslav Melichar ◽  
František Švec
Keyword(s):  
Uric Acid ◽  
Biological Fluids ◽  
Human Saliva ◽  
Medline Search ◽  
Non Invasive ◽  
The Past ◽  
Recent Trends ◽  
Pre Treatment ◽  
Single Publication

AbstractThis review summarizes and critically evaluates the published approaches and recent trends in sample pre-treatment, as well as both separation and non-separation techniques used for the determination of uric acid (UA) in saliva. UA is the final product of purine nucleotide catabolism in humans. UA concentrations in biological fluids such as serum, plasma, and urine represent an important biomarker of diseases including gout, hyperuricemia, or disorders associated with oxidative stress. Previous studies reported correlation between UA concentrations detected in saliva and in the blood. The interest in UA has been increasing during the past 20 years from a single publication in 2000 to 34 papers in 2019 according to MEDLINE search using term “uric acid in saliva”. The evaluation of salivary UA levels can contribute to non-invasive diagnosis of many serious diseases. Increased salivary UA concentration is associated with cancer, HIV, gout, and hypertension. In contrast, low UA levels are associated with Alzheimer disease, progression of multiple sclerosis, and mild cognitive impairment.

A Fluorometric Ferric Chloride Method for Determining Cholesterol in Cerebrospinal Fluid and Serum

1970 ◽  
Vol 16 (6) ◽  
pp. 472-476 ◽  
Author(s):  
Elizabeth B Solow ◽  
L W Freeman
Keyword(s):  
Cerebrospinal Fluid ◽  
Ferric Chloride ◽  
Rapid Determination ◽  
Biological Fluids ◽  
The Past ◽  
Highly Sensitive ◽  
Chloride Method ◽  
Cholesterol Measurement

Abstract Sensitive or simple methods for the rapid determination of cholesterol in biological fluids have been developed during the past 10 years. Sensitivity has been increased by fluorimetry of the Lieberman—Burchard reaction for cholesterol. Measurement of the reaction of cholesterol with ferric chloride is simpler. Still, there are great differences between the results when different methods are used to measure the microquantities of cholesterol present in small volumes of serum or cerebrospinal fluid. In the proposed method, the simpler ferric chloride technique has been made highly sensitive by use of fluorometry. As little as 100 µl of cerebrospinal fluid, containing less than 1 µg of cholesterol, may be used, and the reaction is stable for as long as 1 h. Interference was negligible from pigments (such as bilirubin and hemoglobin), certain drugs, and ionic substances that might be expected to affect fluorescence.

scholarly journals Determination of adenine nucleotides and their metabolites in human saliva.

2000 ◽  
Vol 47 (3) ◽  
pp. 877-879 ◽  
Author(s):  
B Kochańska ◽  
R T Smoleński ◽  
N Knap
Keyword(s):  
Uric Acid ◽  
Individual Variation ◽  
Adenine Nucleotides ◽  
Human Saliva ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Healthy Individuals ◽  
Clinical Value

The profile and normal concentrations of nucleotide metabolites in human saliva and reproducibility of these determinations were analyzed. Samples of human saliva collected from healthy individuals at weekly intervals, were deproteinized and analysed for the content of adenine nucleotides and their metabolites by reversed-phase HPLC. Initial ATP, hypoxanthine and uric acid concentrations were 0.52 +/- 0.15 microM, 1.91 +/- 0.37 microM and 184 +/- 22 microM respectively. A substantial individual variation persisted within 3 weeks of sampling excepted hypoxanthine which showed some unrelated variations. Determination of nucleotides and their catabolites in saliva due to its simplicity and reproducibility, may be of clinical value in diagnosis of local or systemic disorders.

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