The effects of follicle-stimulating hormone receptor (FSHR) -29 and Ser680Asn polymorphisms in IVF/ICSI

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Chrysa Paschalidou ◽  
Elli Anagnostou ◽  
Despoina Mavrogianni ◽  
Rami Raouasnte ◽  
Nikiforos Klimis ◽  
...  
Keyword(s):  
Hormone Receptor ◽  
Follicle Stimulating Hormone ◽  
Promoter Polymorphism ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Vitro Fertilization ◽  
Polymerase Chain ◽  
Stimulating Hormone

AbstractBackgroundThe aim of this study was to analyze two different polymorphisms, Ser680Asn and -29 (G>A) promoter polymorphism, of the follicle-stimulating hormone receptor (FSHR) gene, individually but also in combination, in a sample of Greek women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).Materials and methodsOne hundred and forty-one women undergoing IVF or ICSI and 94 controls were genotyped by real-time polymerase chain reaction (RT-PCR) for the two FSHR polymorphisms. The association of the alleles with the clinical, biochemical and other parameters concerning the controlled ovarian stimulation (COS) protocol and outcome was investigated, as well as the pregnancy rate.ResultsThe study of each polymorphism individually revealed a positive correlation of the SerSer genotype (Ser680Asn polymorphism) with higher luteinizing hormone (LH) levels on the third day of the menstrual cycle. On the other hand, the A allele for the -29 (G>A) promoter polymorphism correlated with the increased number and quality of cumulus-oocyte complexes (COCs). No differences were detected when the different genotypes of the two polymorphisms were combined – the population study was grouped according to the number of polymorphic alleles they carried (0–4 alleles). Women who presented all polymorphic alleles, AsnAsn/AA, exhibited the lowest LH levels (2.62 ± 0.68 mIU/L), but were rarely detected (n = 2, 1.4% of the studied population).ConclusionsThe data from this study reflect that the investigation of the combination of polymorphisms, such as FSHR -29 and Ser680Asn, could offer a valuable tool in order to evaluate and anticipate the outcome of the ovulation induction protocols, especially in the group of patients with failed attempts.

scholarly journals Association of estrogen, progesterone and follicle stimulating hormone receptor polymorphisms with in vitro fertilization outcomes

2018 ◽  
Vol 64 (4) ◽  
pp. 260-265 ◽  
Author(s):  
Vijaya Ganesh ◽  
Vettriselvi Venkatesan ◽  
Teena Koshy ◽  
Sanjeeva Nellapalli Reddy ◽  
Suruli Muthumuthiah ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Hormone Receptor ◽  
Follicle Stimulating Hormone ◽  
Vitro Fertilization ◽  
Stimulating Hormone

scholarly journals Genetic Profile of Follicle Stimulating Hormone Receptor (rFSH) in Madrasin Crossbreed Cattle

2021 ◽  
Vol 4 (1) ◽  
pp. 91
Author(s):  
Aisyah Shaumanur Artha Hidayah ◽  
Budi Utomo ◽  
Imam Mustofa
Keyword(s):  
Hormone Receptor ◽  
Profile Analysis ◽  
Follicle Stimulating Hormone ◽  
Dna Amplification ◽  
Genetic Profile ◽  
Chain Reaction ◽  
Pcr Method ◽  
Dna Fragment ◽  
Polymerase Chain ◽  
Stimulating Hormone

The aim of this study was to know about genetic profile of Folicle Stimulating Hormone Receptor (rFSH) in Madrasin crossbreed between Madura breed and Limousin breed (Madrasin). The research are using whole blood samples from Madura breed, Limousin breed, and Madrasin crossbreed. Madura breed and Madrasin crossbreed sample were taken from Galis, Pamekasan, East Java and Limousin breed is taken from Balai Embrio Ternak (BET) Cipelang, Bogor, West Java. Samples were used for genetic profile analysis such as DNA amplification with Polymerase Chain Reaction (PCR) method and DNA sequencing in Institute of Tropical Disease, Universitas Airlangga. The result showed that DNA fragment of rFSH is 211bp. In conclusion, this study can detect rFSH gene Madrasin crossbreed which is polymorpism.

P–588 Follicle-stimulating hormone receptor genotype and its influence on the results of double ovarian stimulation in IVF cycles

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
M Horta. Foronda ◽  
B Lledó ◽  
J A Ortiz ◽  
A Fuentes ◽  
A Cascales ◽  
...  
Keyword(s):  
Ovarian Stimulation ◽  
Hormone Receptor ◽  
Luteal Phase ◽  
Follicle Stimulating Hormone ◽  
Follicular Phase ◽  
Fsh Receptor ◽  
Vitro Fertilization ◽  
Urinary Fsh ◽  
Stimulating Hormone

Abstract Study question Does the follicle-stimulating hormone receptor (FSHR) genotype influence the results of the ovarian stimulation treatment in the luteal phase? Summary answer All patients undergoing in-vitro fertilization benefit from luteal phase ovarian stimulation, regardless of their follicle-stimulating hormone receptor genotype. What is known already Previous studies suggest that FSH receptor polymorphism in position 680 influences the response to ovarian stimulation in the luteal phase. It was observed that patients with SS genotype seems to require a higher dose to obtain an optimal ovarian response. Later, it was reported that, in patients with SS genotype, a better performance seems to be obtained by administering highly purified urinary FSH while, in SN patients, better results were obtained with recombinant FSH. In patients with NN genotype, no differences were found. Our aim was to test whether this concept is applicable to ovarian stimulation in the luteal phase. Study design, size, duration One hundred and thirty-four patients were included in a retrospective study between July 2017 and September 2020. In these patients, a double stimulation protocol was carried out and the FSH receptor was genotyped either as part of the pre-treatment fertility tests or for the current study. Patients with a double stimulation treatment who could not be genotyped were excluded from the analysis. Participants/materials, setting, methods To genotype the 680 position of the FSH receptor, a real-time PCR for allelic discrimination was carried out using StepOnePlus™ Real-Time PCR System (Applied Biosystems™. Ref: 4376600). Non-parametic tests were used to study the differences between the groups. They were performed with the software R Statistical Software, version 4.0.3. Main results and the role of chance The results of ovarian stimulation in the luteal phase were better compared to the conventional follicular phase. Statistically significant differences (p < 0.001) were found in the number of retrieved oocytes (5.06 versus 3.51), retrieved MII (4.13 versus 2.91), fertilized oocytes (3.22 versus 1.81) and blastocysts formed (1.79 versus 0.62). Furthermore, these differences remained regardless of the genotype for the 680 position of the FSH receptor in all groups (p < 0.05). In addition, better results were obtained in the luteal phase in patients who have been stimulated with the type of gonadotropin that already had better performance in the follicular phase for its genotype, that is, highly purified urinary FSH in SS patients and recombinant FSH in SN patients, compared to other types of gonadotropin (p < 0.05). We also observed that stimulation in the luteal phase lasts longer and consume more gonadotropins than in the follicular phase. This is especially notable in the case of patients with SS genotype, who required slightly higher consumption of gonadotropins compared to the other genotypes in the luteal phase, as had previously been observed in the follicular phase for this genotype. Limitations, reasons for caution The retrospective study design and the sample size could be a limitation. Furthermore, we cannot determine whether the improvement in luteal phase performance is related to differences in the physiological environment between phases of the cycle or is caused by a possible activation of the ovary from the previous stimulation. Wider implications of the findings: All patients undergoing in-vitro fertilization seems to benefit from luteal phase ovarian stimulation, regardless of their genotype for FSHR. In addition, the pharmacogenetic recommendation when choosing the type of FSH for ovarian stimulation should be the same both in the follicular phase and in the luteal phase. Trial registration number Not applicable

scholarly journals Superovulation and expression of follicle-stimulating hormone receptor in young rabbit females

2017 ◽  
Vol 25 (2) ◽  
pp. 167 ◽  
Author(s):  
Hao Zhang ◽  
Guo Hu Cheng ◽  
Yong Jun Li ◽  
Miao Yin Cai ◽  
Hai Yan Guo ◽  
...  
Keyword(s):  
Hormone Receptor ◽  
Quantitative Polymerase Chain Reaction ◽  
Mrna Level ◽  
Follicle Stimulating Hormone ◽  
Young Rabbit ◽  
Fertilisation Rate ◽  
Maturation Rate ◽  
Polymerase Chain ◽  
Stimulating Hormone

<p>To optimise the use of juvenile in vitro embryo transfer technologies in young rabbit females, superovulation was performed in New Zealand White young rabbit females at different ages and the expression mode of follicle-stimulating hormone receptor (FSHR) was explored using real-time quantitative polymerase chain reaction, and in vitro maturation (IVM) together with fertilisation (IVF) was conducted immediately after superovulation. The results showed that (1) the age factor significantly affected superovulation in young rabbit females, with 60 d as an optimal age; (2) the mRNA level of FSHR exhibited a rising trend, though it was lower at 30 to 40 d of age; (3) the maturation rate of the oocytes from 60 d old rabbits was significantly higher than in those from 50 d old rabbits; (4) the fertilisation rate of oocytes was not significantly different among rabbits 50, 60 and 70 d old.</p>

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