scholarly journals The Ability of Honey Bee Drones to Ejaculate

2015 ◽  
Vol 59 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Krystyna Czekońska ◽  
Bożena Chuda-Mickiewicz
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Reproductive Organs ◽  
Seminal Vesicles ◽  
Analysis And Evaluation ◽  
Anatomical Changes ◽  
Manual Method ◽  
Delayed Development

Abstract The effectiveness of two methods of collecting semen from honeybee Apis mellifera drones was compared, and the reasons for problems with ejaculating semen were analysed. Among 275 drones, 100 were stimulated to release semen using a manual method, 100 with the use of chloroform, and from 75 drones the reproductive organs were dissected for analysis and evaluation. It was found that the principal causes of problems that drones had with ejaculating their semen were anatomical changes or a delay in the development of the mucus glands. It was also found that the method employing chloroform was less efficient in the first phase of eversion of the endophallus, compared with the manual method. The method with the use of chloroform allows the determination of the proportion of drones, which do not evert the endophallus because of poor or delayed development of mucus glands, as well as the proportion of drones which evert the organ, but do not ejaculate semen because of the absence of semen in the seminal vesicles.

THE GROWTH OF THE VENTRAL AND DORSOLATERAL PROSTATE, THE COAGULATING GLANDS AND THE SEMINAL VESICLES IN FORCE-FED HYPOPHYSECTOMIZED CASTRATED ADRENALECTOMIZED RATS INJECTED WITH CORTISONE AND/OR INSULIN

1972 ◽  
Vol 71 (1) ◽  
pp. 179-190 ◽  
Author(s):  
Lars-Eric Tisell ◽  
Lennart Angervall
Keyword(s):  
Histological Examination ◽  
Anterior Pituitary ◽  
Adrenal Glands ◽  
Reproductive Organs ◽  
Seminal Vesicles ◽  
The Other ◽  
Protamine Zinc Insulin ◽  
Adrenalectomized Rats ◽  
Stimulation Of

ABSTRACT The growth of the ventral and dorsolateral prostate, the coagulating glands and the seminal vesicles was studied in force-fed hypophysectomized castrated adrenalectomized rats following daily injections for fourteen days of protamine zinc insulin or cortisone acetate alone or in combination. Cortisone was given in daily doses of 3 mg and insulin was administered in increasing daily doses of protamine zinc insulin up to 8 IU. Cortisone alone induced slight histological stimulation of the epithelium of the coagulating glands, while no stimulation was demonstrated in the other accessory reproductive organs. After insulin alone the weight of the accessory reproductive organs was slightly increased but no stimulation was demonstrated histologically. Cortisone and insulin given in combination induced distinct signs of stimulation of all the accessory reproductive organs as assesed by histological examination and weight determination of the organs. The results indicate that in the rat the growth stimulating effect of cortisone on the male accessory reproductive organs is markedly decreased or abolished in the absence of the anterior pituitary. Insulin can act synergistically with cortisone in promoting the growth of the accessory reproductive organs through effects which are not dependent on the presence of the adrenal glands or the anterior pituitary.

Separation and Determination of Seminal Plasma and Sperm Amino Acids of the Honey Bee, Apis Mellifera

1960 ◽  
Vol 53 (6) ◽  
pp. 841-843 ◽  
Author(s):  
A. F. Novak ◽  
M. S. Blum ◽  
S. Taber ◽  
J. A. Liuzzo
Keyword(s):  
Amino Acids ◽  
Apis Mellifera ◽  
Honey Bee ◽  
Seminal Plasma

Morphological diversity and racial determination of the honey bee (Apis mellifera L.) population in the Republic of Macedonia

2009 ◽  
Vol 48 (3) ◽  
pp. 196-203 ◽  
Author(s):  
Aleksandar Uzunov ◽  
Hrisula Kiprijanovska ◽  
Sreten Andonov ◽  
Mirce Naumovski ◽  
Aleš Gregorc
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Morphological Diversity ◽  
Republic Of Macedonia ◽  
The Republic

scholarly journals Application of Next Generation Semiconductor-Based Sequencing for the Identification of Apis mellifera Complementary Sex Determiner (csd) Alleles from Honey DNA

Insects ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 868
Author(s):  
Samuele Bovo ◽  
Anisa Ribani ◽  
Valerio Joe Utzeri ◽  
Valeria Taurisano ◽  
Giuseppina Schiavo ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Hypervariable Region ◽  
Next Generation ◽  
Sequencing Data ◽  
Massive Sequencing ◽  
History Of ◽  
Genome Information ◽  
Csd Gene

The complementary sex determiner (csd) gene plays an essential role in the sex determination of Apis mellifera L. Females develop only if fertilized eggs have functional heterozygous genotypes at this gene whereas males, being haploids, are hemizygous. Two identical csd alleles produce non vital males. In light of the recent decline in honey bee populations, it is therefore important to monitor the allele variability at this gene. In this study, we tested the application of next generation semiconductor-based sequencing technology (Ion Torrent) coupled with environmental honey DNA as a source of honey bee genome information to retrieve massive sequencing data for the analysis of variability at the hypervariable region (HVR) of the csd gene. DNA was extracted from 12 honey samples collected from honeycombs directly retrieved from 12 different colonies. A specifically designed bioinformatic pipeline, applied to analyze a total of about 1.5 million reads, identified a total of 160 different csd alleles, 55% of which were novel. The average number of alleles per sample was compatible with the number of expected patrilines per colony, according to the mating behavior of the queens. Allele diversity at the csd could also provide information useful to reconstruct the history of the honey.

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