Neuroprotective role of 6-Gingerol-rich fraction of Zingiber officinale (Ginger) against acrylonitrile-induced neurotoxicity in male Wistar rats

2018 ◽  
Vol 30 (3) ◽  
Author(s):  
Ebenezer Olatunde Farombi ◽  
Amos Olalekan Abolaji ◽  
Babatunde Oluwafemi Adetuyi ◽  
Olaide Awosanya ◽  
Mobolaji Fabusoro
Keyword(s):  
Brain Damage ◽  
Corn Oil ◽  
Zingiber Officinale ◽  
Protective Role ◽  
Male Rats ◽  
Cortex Lesion ◽  
Male Wistar Rats ◽  
Group B ◽  
Factor Α

Abstract Background Acrylonitrile (AN) is a neurotoxin that is widely used to manufacture synthetic fibres, plastics and beverage containers. Recently, we reported the ameliorative role of 6-gingerol-rich fraction from Zingiber officinale (Ginger, GRF) on the chlorpyrifos-induced toxicity in rats. Here, we investigated the protective role of GRF on AN-induced brain damage in male rats. Methods Male rats were orally treated with corn oil (2 mL/kg, control), AN (50 mg/kg, Group B), GRF (200 mg/kg, Group C), AN [50 mg/kg+GRF (100 mg/kg) Group D], AN [(50 mg/kg)+GRF (200 mg/kg) Group E] and AN [(50 mg/kg)+N-acetylcysteine (AC, 50 mg/kg) Group F] for 14 days. Then, we assessed the selected markers of oxidative damage, antioxidant status and inflammation in the brain of rats. Results The results indicated that GRF restored the AN-induced elevations of brain malondialdehyde (MDA), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) and Nitric Oxide (NO) levels. GRF also prevented the AN-induced depletion of brain glutathione (GSH) level and the activities of Glutathione S-transferase (GST), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in rats (p<0.05). Furthermore, GRF prevented the AN-induced cerebral cortex lesion and increased brain immunohistochemical expressions of Caspases-9 and -3. Conclusions Our data suggest that GRF may be a potential therapeutic agent in the treatment of AN-induced model of brain damage.

scholarly journals THE EFFECTS OF KEFIR ON THE INFLAMATORY STATUS AND THYROID FUNCTION (EXPERIMENTAL STUDY ON WISTAR RATS AFTER EXPOSED TO CHLORPYRIFOS)

2016 ◽  
Vol 9 (5) ◽  
pp. 165 ◽  
Author(s):  
Rasipin Rasipin ◽  
Edi Dharmana ◽  
Suharyo Hadisaputro ◽  
Suhartono .
Keyword(s):  
Thyroid Function ◽  
Wistar Rats ◽  
Thyroid Dysfunction ◽  
Corn Oil ◽  
Thyroid Stimulating Hormone ◽  
Male Rats ◽  
Tnf Α ◽  
Male Wistar Rats ◽  
Α Level ◽  
Factor Α

ABSTRACTObjective: Goiter is an enlarged thyroid gland remained a health problem in the agricultural areas. Chlorpyrifos (CPF) is a pesticide widely used byfarmers. Previous studies proved that CPF exposure caused thyroid dysfunction. The objective of this study was to evaluate the effects of kefir on theinflammatory status and thyroid function in male Wistar rats after exposed to CPF using biochemical and histopathological assays.Methods: Male rats were divided into 4 groups, i.e., CPF 5+kefir (5 mg/kg+3.6 ml/200 g, respectively), CPF 5 (5 mg/kg), corn oil (CO 1 ml/200 g), andnegative control (NC: Without CPF, CO, and kefir).Results: Kefir supplementation dose 3.6 ml/200 g once a day for 28 days in the rats after exposed to CPF dose 5 mg/kg once a day for 14 days, inCPF 5+kefir as compared to CPF 5: Significantly (p<0.05) decreased serum tumor necrosis factor-α (TNF-α) level; significantly (p<0.01) maintainedserum levels of tumor growth factor-β (TGF-β) and thyroid stimulating hormone (TSH) not to decrease; not significant (p>0.05) decreased the level ofinterleukin-1β, cluster of differentiation-26 expression and level of T serum; not significant (p>0.05) maintained the level of anti-thyroid peroxidasenot to decrease; and not significant (p>0.05) increased the apoptosis index. This study suggests that CPF exposure causes the inflammatory processwhich leads to thyroid dysfunction.4Conclusion: Kefir supplementation significantly decreased the level of TNF-α and maintained the levels of TGF-β and TSH not to decrease, possible toreduce the inflammatory and thyroid dysfunction processes caused by exposure to CPF in experimental animals.Keywords: Kefir, Chlorpyrifos, Inflammation, Thyroid function. 

The protective role of Zingiber officinale extract against methyl thiophanate induced toxicity in male rats

2017 ◽  
Author(s):  
Rachid Mosbah ◽  
Malika Bahri ◽  
Said Azzoug ◽  
Zouhir Djerrou ◽  
Alberto Mantovani
Keyword(s):  
Zingiber Officinale ◽  
Protective Role ◽  
Male Rats ◽  
Methyl Thiophanate

scholarly journals Protective Role of Lycopene on Hormonal Profile and Posttesticular Functions of Male Rat Exposed to Sublethal Doses of Cyermethrin

2019 ◽  
pp. 1-9
Author(s):  
Adetutu Olubunmi Obulor ◽  
Eme Efioanwan Orlu
Keyword(s):  
Protective Role ◽  
Male Rats ◽  
Male Rat ◽  
Eosin Y ◽  
Environmental Biology ◽  
Hormonal Profile ◽  
Sublethal Doses ◽  
Group B ◽  
Rivers State

Aim: The aim of the study is to evaluate the Protective role of Lycopene on hormonal profile and posttesticular functions of male rats exposed to sublethal doses of Cypermethrin. Study Design: The study was a completely randomized design employing relevant statistical tools for analysis and interpretation. Place and Duration of Study: The study was carried out in the Reproductive Physiology and Genetics Research Laboratory of the Department of Applied and Environmental Biology, Rivers State University, Rivers State. The experiment lasted for 35 days. Methodology: For the sperm morphology assay, sperm reserves and hormonal profiling, semen samples were drawn from the caudal epididymis with a syringe and placed on a clean glass slide. A drop of freshly prepared eosin-Y was added to make a thin smear and examined under the microscope for morphological abnormalities. A portion of the testis and epididymidis was homogenized separately with sharp pointed scissors in normal (physiological) saline. The suspension was mixed and strained through a double layer of sterile cheese cloth into graduated test tubes. All the samples were covered and stored for 24 hours at 40C. A dilution was made for counting in Neubauer haemocytometer. The hormonal concentration was determined using the Randox Monza Laboratories assay kit from Co-Atrim, United Kingdom.  Results: Results of oral administration of Cypermethrin and co-administration of lycopene in rats showed Group G co-administered pure Lycopene had the lowest sperm head abnormalities of 3.5%, group B administered Cypermethrin only had the highest head abnormalities of 44.7%. Similarly, Group G, co-administered pure Lycopene had the lowest percentage of tail abnormalities of 10.1%  while Group B had the highest percentage of sperm tail abnormalities of 32.4%. There was a was significant (p<0.001) decrease in concentration of all androgens considered in group B administered Cypermethrin only. Conclusion: Exposure to Cypermethrin only as in group B disrupted the production of all androgens considered, increased the percentage of abnormal spermatozoa, reduced sperm motility, viability and sperm reserves. However, results recorded from the co-administration of Solanum lycopersicum and pure lycopene, in groups C-G indicate the protective role of this potent antioxidant on spermatogenesis and hormonal profile.

Excitotoxic brain damage involves early peroxynitrite formation in a model of Huntington’s disease in rats: Protective role of iron porphyrinate 5,10,15,20-tetrakis (4-sulfonatophenyl)porphyrinate iron (III)

Neuroscience ◽  
2005 ◽  
Vol 135 (2) ◽  
pp. 463-474 ◽  
Author(s):  
V. Pérez-De La Cruz ◽  
C. González-Cortés ◽  
S. Galván-Arzate ◽  
O.N. Medina-Campos ◽  
F. Pérez-Severiano ◽  
...  
Keyword(s):  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Brain Damage ◽  
Protective Role

Acute and subchronic co-administrations to cadmium, diazinon and selenium induce apparent osteoporotic symptoms in adult male rats

Biologia ◽  
2014 ◽  
Vol 69 (10) ◽  
Author(s):  
Hana Ďúranová ◽  
Monika Martiniaková ◽  
Ivana Boboňová ◽  
Radoslav Omelka ◽  
Robert Stawarz ◽  
...  
Keyword(s):  
Adult Male ◽  
Osteoporotic Fractures ◽  
Bone Diseases ◽  
Male Rats ◽  
Control Group ◽  
Femoral Bone ◽  
Negative Effects ◽  
Young Males ◽  
Male Wistar Rats ◽  
Group B

AbstractCadmium (Cd) and diazinon (DZN) are known to be environmental risk factors for various bone diseases including osteoporosis. Selenium (Se), an essential constituent of many antioxidant enzymes, has in higher concentrations negative effects on the bone. The present study was aimed to investigate possible changes in femoral bone of adult male rats after their acute and subchronic exposures to Cd, DZN and Se. A total of 30 male Wistar rats were randomized into three experimental groups. The rats in the group A (4-months-old) were injected intraperitoneally with a mixture of 2 mg CdCl2 kg−1, 20 mg DZN kg−1 and 2 mg Na2SeO3 kg−1 body weight and killed 36 h after xenobiotics had been injected. In the group B, young males (1-month-old) were administered with a combination of 30 mg CdCl2 L−1, 40 mg DZN L−1 and 5 mg Na2SeO3 L−1 in their drinking water, for 90 days. Ten 4-months-old males without toxicant supplementation served as a control group (C). After treatment period, detailed histological analysis of femoral bone was performed in each group. Our results revealed apparent osteoporotic symptoms (resorption lacunae, osteoporotic fractures) in rats from groups A and B. Moreover, histomorphometrical evaluation showed reduced bone vascularization (constricted primary osteons’ vascular canals and Haversian canals) and weakness mechanical properties of bones (smaller size of the secondary osteons) in these rats in comparison with those of the control group. Our study demonstrates for the first time that acute and subchronic co-administrations to Cd, DZN and Se induce evident manifestation characteristics of osteoporosis in male rats.

scholarly journals The Effect of the mGlu8 Receptor Agonist, S-3,4-DCPG on Acquisition and Expression of Morphine-induced Conditioned Place Preference in Male rats

2021 ◽  
Author(s):  
Nazanin Kahvandi ◽  
Zahra Ebrahimi ◽  
Seyed Asaad Karimi ◽  
Siamak Shahidi ◽  
Iraj Salehi ◽  
...  
Keyword(s):  
Conditioned Place Preference ◽  
Metabotropic Glutamate Receptors ◽  
Preference Test ◽  
Place Preference ◽  
Male Rats ◽  
Dependent Manner ◽  
Metabotropic Glutamate ◽  
Male Wistar Rats ◽  
Dose Dependent

Abstract Background: The nucleus accumbens (NAc) plays a principal role in drug reward. It has been reported that metabotropic glutamate receptors (mGlu receptors) play a key role in the rewarding pathway(s). Previous studies have shown the vast allocation of the different types of mGlu receptors, including mGlu8 receptors, in regions that are associated with opioid rewards, such as the NAc. The aim of the present study was to evaluate the role of mGlu8 receptors within the NAc in the acquisition and expression phases of morphine induced conditioned place preference (CPP). Adult male Wistar rats were bilaterally implanted by two cannulas' in the NAc and were evaluated in a CPP paradigm. Selective mGlu8 receptor allosteric agonist (S-3,4-DCPG) was administered at doses of 0.03, 0.3, and 3 μg/0.5 μL saline per side into the NAc on both sides during the 3 days of morphine (5 mg/kg) conditioning (acquisition) phase, or before place preference test, or post-conditioning (expression) phase of morphine-induced CPP. Results: The results revealed that intra-accumbal administration of S-3,4-DCPG (0.3 and 3 μg) markedly decreased the acquisition in a dose-dependent manner but had no effect on expression of morphine-induced CPP. Conclusions: The findings suggest that activation of mGlu8 receptors in the NAc dose-dependently blocks the establishment of morphine-induced CPP and reduces the rewarding properties of morphine which may be related to the glutamate activity into the NAc and in reward pathway(s). These data suggest that mGlu8 receptor may be involved in conditioned morphine reward.

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