scholarly journals Microbiological Findings in Deep Caries Lesions

2016 ◽  
Vol 63 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Irena Kuzmanović Radman ◽  
Aleksandra Djeri ◽  
Adriana Arbutina ◽  
Jelena Milašin

SUMMARY Introduction Caries is one of the most significant and widespread oral diseases. It has been confirmed that dental plaque, i.e. microorganisms in it, are the most important factor in the development of dental caries. Caries profunda represents deep carious lesion from where bacterial toxins may affect pulp through dentinal tubules. The aim of this study is to assess the efficacy of indirect pulp capping based on microbiological findings of bacteria present in deep carious lesions before and after the treatment. Material and Methods The clinical study included 29 patients of both genders, aged 16 to 40 and 45 permanent teeth with deep caries lesions. The first microbiological sample was taken after cavity preparation and removal of soft dentin from the bottom of the cavity. The second sample was taken after the removal of temporary filling and calcium hydroxide paste 60 days after the indirect pulp capping treatment. The collected samples were stored in special sterile micro tubes (Eppendorf) and kept at the temperature of −80°C until microbiological analysis was performed. Samples were tested for the presence of the following microorganisms: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Enterococcus faecalis using the multiplex polymerase chain reaction (PCR) method. Results The results showed that prior to the treatment of deep carious lesions the most common species was E. faecalis (80% of samples), followed by A. actinomycetemcomitans (32% of samples), while the least common was P. gingivalis (16% of samples). After the treatment with products based on calcium hydroxide, E. faecalis was registered in 18% of samples, A. actinomycetemcomitans in 16% of samples and P. gingivalis was not registered in any sample. Conclusion The most common bacterial species in teeth with deep caries lesions was E. faecalis, whereas A. actinomycetemcomitans and P. gingivalis were found in lower number of samples.

2016 ◽  
Vol 9 (3) ◽  
pp. 140 ◽  
Author(s):  
Rafeza Sultana ◽  
Mozammal Hossain ◽  
Md. Shamsul Alam

<p>The maintenance of pulp vitality and conduction of reparative dentin can be possible by indirect pulp capping with mineral trioxide aggregate (MTA) and calcium hydroxide as pulp capping agents. The objective of the study is to assess the clinical and radiological outcomes of MTA and calcium hydroxide as indirect pulp capping agents in deep carious lesions of permanent teeth. The present study included 50 permanent teeth having deep carious lesions with reversible pulp status were selected and then randomly divided into two groups of 25 teeth in a group. Standard indirect pulp capping procedures were followed. Patients were recalled at 3, 6 and 12 months interval to assess postoperative pain, the vitality of the pulp and formation of reparative dentin. In all observation periods, MTA showed more capable of reducing pain and maintain pulp vitality which was statistically significant than that of calcium hydroxide. At 12 months observation period, 24 teeth (96%) of MTA and 19 teeth (76%) of calcium hydroxide showed reparative dentin formation. It can be concluded that MTA is more effective than that of calcium hydroxide.</p><p> </p>


2017 ◽  
Vol 42 (5) ◽  
pp. 470-477 ◽  
Author(s):  
U Koc Vural ◽  
A Kiremitci ◽  
S Gokalp

SUMMARY Objective: This clinical study aimed to assess the efficacies of mineral trioxide aggregate (MTA) and calcium hydroxide [Ca(OH)2] in the treatment of deep carious lesions by the direct complete caries removal technique. Methods and Materials: A total of 100 permanent molar/premolar teeth were capped with either Ca(OH)2 (n=49) or MTA (n=51) and restored with composite resin in 73 patients. Periapical radiographs were acquired prior to the treatment as well as at six, 12, and 24 months posttreatment. Two calibrated examiners performed the clinical and radiographic assessment of the periapical pathology and pulpal symptoms. Intergroup comparisons of the observed values were performed using the Fisher exact test. Significance was predetermined at α = 0.05. Results: The recall rates were 100% at six and 12 months posttreatment and 98.6% at 24 months posttreatment. Four teeth capped with Ca(OH)2 (two each at six and 12 months posttreatment) and two capped with MTA (one each at 12 and 24 months posttreatment) received endodontic emergency treatment because of symptoms of irreversible pulpitis, which were clinically and/or radiographically established. There were no significant differences in pulp vitality between the two pulp-capping agents at six, 12, or 24 months posttreatment (p=0.238, p=0.606, and p=0.427, respectively). Conclusions: Both pulp-capping materials were found to be clinically acceptable at 24 months posttreatment.


2020 ◽  
Vol 10 (1) ◽  
pp. 6-12
Author(s):  
Fahd AA Karim ◽  
Asma Sultana ◽  
Hanann Shiekh ◽  
Md Ashif Iqbal ◽  
Puja Shrestha ◽  
...  

Background:Pulp capping is a treatment that enables pulp to recover and maintain its vitality and function by applying a protective agent to an exposed pulp. Ca (OH)2 has been considered the gold standard for this procedure. Despite its wide use, Ca (OH)2 is not ideally suited for pulp capping. Recently ledermix cement used for indirect pulp capping material in the outer world broadly. Objective: To assess the clinical and radiological evidence of the formation of secondary dentin and compare the degree of short time post operating pain after indirect pulp treatment. Methods: A Randomized control trial in vivo study was directed in the Department of Conservative Dentistry and Endodontics, Faculty of Dentistry, Bangabandhu Sheikh Mujib Medical University for one year (January 2012-January 2013). We took the patients with 40 deep carious permanent teeth as a sample. The selected teeth were divided into two test groups. All odd number teeth (20) were included in group-1(where pulp capping was done with ledermix cement). All even number teeth (20) were included in group-2(where pulp capping was done with Ca(OH)2). We assessed all the patients clinically and radiographically at the interval of 3, 6, and 12 months simultaneously for the assessment of dentinal bridge formation and also collected data was analyzed using statistical package for social science (SPSS) version 17. Results: Among the full scale 40 models, ledermix (20) and Ca(OH)2(20), minor to direct irritaion of the teeth was experienced premium to treatment and found comparable in the two gatherings. Regardless, after 24 hrs of treatment, this value was shockingly higher in the Ca(OH)2 (p<0.01)group than in the Ledermix gathering (p < 0.001, t-test). Following 7 days, there was no basic differentiation between them. Follow up evaluation revealed, failure of 3 teeth treated with Ca(OH)2 and 2 teeth treated with ledermix due to pain and swelling. The remaining 35 teeth radiated an impression of being clinically and radiologically successful a year postoperatively. Conclusion: No post-operative pain was experienced in both groups in the long term. However, within the first hours after cementation, there was a sensation of pain significantly higher in the Ca(OH)2 group than in the Ledermix group. Ledermix can be considered a predictable treatment and may be an alternative to calcium hydroxide in indirect pulp capping. Update Dent. Coll. j: 2020; 10 (1): 6-12


Author(s):  
K. E. Selvendran ◽  
A. Shafie Ahamed ◽  
Madhuram Krishnamurthy ◽  
V. Naveen Kumar ◽  
Vignesh Guptha Raju

Aim: To evaluate the clinical success of Calcium Hydroxide (CH), Mineral trioxide aggregate (MTA) and Biodentine as pulp capping materials for Direct pulp capping in carious molars. Materials and Methods: Thirty-six molars of thirty-six patients with deep caries lesions, diagnosed with reversible pulpitis were subjected to direct pulp capping treatment. They were randomly divided into three groups, Biodentine (12 teeth) or MTA group (12 teeth) or CH group (12teeth). Simple randomization of three was employed to allocate the treatment materials. Patients were recalled at one, three and six months to evaluate the clinical success of the treatment outcome. Results: In clinical trial/study, the pulp capping materials gave different success rate, 91.67% success in the Biodentine group, 75% success in the MTA group and 41.67% success in Calcium Hydroxide group. Conclusion: In our study the materials tested at 1 month, 3 month and 6-month follow-up, Calcium Silicate materials are better than calcium hydroxide and comparably Biodentine is better than MTA for Direct pulp capping. Clinical Significance: The findings of this clinical trial could promote the reliability of pulp capping materials for treatment of deep carious lesions by conservative approach rather than opting endodontic management.


2019 ◽  
pp. 77-83
Author(s):  
N.V. Yanko

The aim of the study was to systematize effective deep caries management methods in children in order to determine the indications for their use. The author used Google search engine to find articles and scientific publications published between 2006 and 2019. Reversible pulpitis is an indication for applying the methods preserving pulp vitality performed under aseptic and antiseptic conditions, provided the absence of decompensated form of caries and chronic somatic pathology. To prevent the accidental pulp exposure, it is better to use a partial excavation of the affected dentin followed by an indirect pulp capping. During direct pulp capping and pulpotomy the bleeding should be arrested within 3-5 min in primary teeth and within 5 min – in permanent ones. Direct pulp capping with MTA is indicated at the spot pulp exposure in permanent teeth, the use of this method in the primary teeth requires further research. Direct pulp capping with 5.25% sodium hypochlorite used as a hemostatic agent and MTA-type cement application under endodontic microscope or pulpotomy with MTA or Biodentine can be applied when treating reversible pulpitis in permanent teeth. In asymptomatic temporary teeth affected by reversible pulpitis full pulpotomy is adviced which is more effective with MTA. A promising direction in deep caries management research is direct and indirect pulp capping of primary teeth with Biodentine.


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