Störung des Nachweises der Ribonucleinsäure-Polymerase in Tabakblättern durch eine Nucleosidase

An enzymatic degradation of ATP, ADP, AMP to adenine was observed in tobacco leaves. This enzymatic activity was concentrated 130 fold. 14C-adenine can interfere with the determination of polymerase by adsorption to filters and bentonite. The degradation of ATP can be avoided by addition of phosphorenol pyruvate and kinase. A weak polymerase activity was found to be associated with particles in leaf extracts, but was not found in the supernatant.

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A Method for the Colorimetric Determination of β-Glucuronidase in Urine, Serum, Tissue; Assay of Enzymatic Activity in Health and Disease

Gastroenterology ◽

10.1016/s0016-5085(59)80003-2 ◽

1959 ◽

Vol 36 (2) ◽

pp. 193-201 ◽

Cited By ~ 46

Author(s):

Julius A. Goldbarg ◽

Esteban P. Pineda ◽

Benjamin M. Banks ◽

Alexander M. Rutenburg

Keyword(s):

Enzymatic Activity ◽

Colorimetric Determination ◽

Health And Disease ◽

Urine Serum

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Determination of phenolic compounds in fresh tobacco leaves by high performance liquid chromatography-ultraviolet/mass spectrometry

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2013.08004 ◽

2014 ◽

Vol 32 (1) ◽

pp. 40 ◽

Cited By ~ 2

Author(s):

Danhong SHEN ◽

Xin LU ◽

Yuwei CHANG ◽

Junjie ZHANG ◽

Yanni ZHAO ◽

...

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Phenolic Compounds ◽

High Performance ◽

Tobacco Leaves

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Fluorimetric Determination Of Mercury (Ii) Based On The Inhibition Of The Enzymatic Activity Of Urease

Analytical Letters ◽

10.1080/00032719408007357 ◽

1994 ◽

Vol 27 (5) ◽

pp. 867-878 ◽

Cited By ~ 22

Author(s):

D. W. Bryce ◽

J. M. Fernández-Romero ◽

M. D. Luque de Castro

Keyword(s):

Enzymatic Activity ◽

Fluorimetric Determination

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Isolation by HPLC of the positional isomers of 61,2n-di-O-α-d-galactopyranosylcyclomaltohexaose (n = 1–6) and determination of their structures by enzymatic degradation

Carbohydrate Research ◽

10.1016/s0008-6215(96)00284-4 ◽

1997 ◽

Vol 297 (4) ◽

pp. 301-307 ◽

Cited By ~ 7

Author(s):

Yasuyo Okada ◽

Yukiko Okazaki ◽

Kyoko Koizumi ◽

Koji Hara ◽

Sumio Kitahata

Keyword(s):

Enzymatic Degradation ◽

Positional Isomers

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A rapid and highly sensitive method for simultaneous determination of bioactive constituents in leaf extracts of six Ocimum species using ultra high performance liquid chromatography-hybrid linear ion trap triple quadrupole mass spectrometry

Analytical Methods ◽

10.1039/c5ay01055a ◽

2016 ◽

Vol 8 (2) ◽

pp. 333-341 ◽

Cited By ~ 5

Author(s):

Renu Pandey ◽

Preeti Chandra ◽

Brijesh Kumar ◽

Bhupender Dutt ◽

Kulwant Rai Sharma

Keyword(s):

Food Processing ◽

High Performance ◽

Ion Trap ◽

Linear Ion Trap ◽

Quadrupole Mass ◽

Bioactive Constituents ◽

Leaf Extracts ◽

Highly Sensitive ◽

Ocimum Species

Ocimum species have tremendous value in pharmaceutical, perfumery, food processing and cosmetic industries, also in traditional rituals and medicines.

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Growth abnormalities in microorganisms exposed to tobacco leaf extracts

Canadian Journal of Microbiology ◽

10.1139/m74-251 ◽

1974 ◽

Vol 20 (11) ◽

pp. 1619-1621 ◽

Cited By ~ 1

Author(s):

S. A. Ghabrial

Keyword(s):

Culture Media ◽

Aqueous Extracts ◽

Leaf Extracts ◽

Cultural Changes ◽

Tobacco Leaves ◽

Tobacco Leaf ◽

Yeast Strains ◽

Extract Medium ◽

Tobacco Extract ◽

Growth Abnormalities

Unusual cultural changes were observed when several bacterial and yeast strains were grown on a medium containing aqueous extracts of tobacco leaves. In 24–48 h incubation on such a medium, several dark-brown papilla-like structures developed on the surface of normally smooth colonies. Moreover, smaller dark-brown refractile globular bodies were observed in the medium in areas beneath and surrounding the microbial colonies with papilla-like structures. Attempts to demonstrate the viability of these globular bodies failed. The cultural abnormalities observed on tobacco extract medium were unique to this medium since they were not detected in a wide variety of culture media tested. The lowest concentration of tobacco extract (dry wt. basis), which is required for the development of this phenomenon, was determined to be 0.5 mg/ml of medium.

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Determination of Free and Bound Maleic Hydrazide Residues in Tobacco by High Performance Liquid Chromatography

Beiträge zur Tabakforschung International/Contributions to Tobacco Research ◽

10.2478/cttr-2013-0632 ◽

1994 ◽

Vol 16 (2) ◽

pp. 57-64 ◽

Cited By ~ 1

Author(s):

BS Dattilo ◽

S Gallo ◽

G Lionetti ◽

SG Rossi

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Quantitative Determination ◽

High Performance ◽

Maleic Hydrazide ◽

Dilute Solution ◽

Tobacco Leaves ◽

Qualitative And Quantitative ◽

Curve Method

AbstractA new method is described for the qualitative and quantitative determination of both free and bound maleic hydrazide residues in tobacco leaves and cigarette filler by high performance liquid chromatography. Analyses were carried out by hydrolyzing samples of ground tobacco with 4 N hydrochloric acid for 40 minutes under reflux followed by sample chromatography, running isocratic elutions with a dilute solution of phosphoric acid. The quantitative determination of maleic hydrazide was performed by light absorption at 320 nm, by the calibration curve method. Recoveries of maleic hydrazide added to tobacco samples were greater than 90 %. The detection limit of the method, determined on ground tobacco leaves, was at least 5 ppm. The results obtained by this procedure and by the ISO standard method no. 4876 are in good accordance.

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