Acetate Concentration and Chloroplast Pyruvate Dehydrogenase Complex in Spinacia oleracea Leaf Cells
1985 ◽
Vol 40
(3-4)
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pp. 182-188
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Keyword(s):
Acetate concentration in spinach (Spinacia oleracea L.) leaf tissue has been determined by direct measurement in a leaf extract to be 70 μᴍ or less. Additional evidence for such low acetate levels came from isotope dilution experiments where the [l-14C]acetate incorporation into fatty acids was assayed using purified chloroplasts in the presence of tissue extracts. Pyruvate dehydrogenase complex activity was shown to be present in spinach chloroplasts and was determined by a newly developed acetyl coenzyme A trapping system (100 nmol acetyl CoA formed per mg chloroplast protein per h). The problems of acetyl CoA synthesis in both compartments, chloroplasts and cytoplasm, are discussed in this context.
2013 ◽
Vol 79
(18)
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pp. 5566-5575
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2015 ◽
Vol 38
(5)
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pp. 895-904
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