scholarly journals Bioactive Compounds of the Volatile Oil of Dracocephalum kotschyi

2007 ◽  
Vol 62 (11-12) ◽  
pp. 793-796 ◽  
Author(s):  
Soodabeh Saeidnia ◽  
Ahmad Reza Gohari ◽  
Abbas Hadjiakhoondi ◽  
Abbas Shafiee
Keyword(s):  
Silica Gel ◽  
Bioactive Compounds ◽  
Column Chromatography ◽  
Spectroscopic Data ◽  
Volatile Oil ◽  
Biologically Active ◽  
Trypanocidal Activity ◽  
H Nmr ◽  
Dracocephalum Kotschyi ◽  
C Nmr

Trypanocidal activity was found in the volatile oil of dried Dracocephalum kotschyi. GCMS analysis determined that the major constituents of the oil were geranial (35.8%), C10H14O (26.6%), limonene (15.8%) and 1,1-dimethoxy decane (14.5%). In order to isolate the unknown biologically active monoterpene, fractionation of the volatile oil was carried out by silica gel column chromatography. The structure of the oxygenated compound was confirmed to be limonene-10-al (C10H14O) by analysis of physical and spectroscopic data (1H NMR, 13C NMR, HMBC and HMQC).

Progesterone Hydroxylation with Colletotrichum Lini AS3. 4486

2011 ◽  
Vol 343-344 ◽  
pp. 1070-1073 ◽  
Author(s):  
Yan Jun Shen ◽  
Hua Sun ◽  
Yu Wan Fu ◽  
Chu Yang Xu ◽  
Min Wang
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Broth Culture ◽  
H Nmr ◽  
Culture Supernatants ◽  
C Nmr

The biotransformation of progesterone by Colletotrichum lini AS3. 4486 was studied. The formation of the product was monitored by HPLC. Product was purified from broth culture supernatants by silica gel column chromatography and identified as 7β,12β-dihydoxylprogesterone with MS, 1H NMR, 13C NMR and NOE.

Iridoids from the stems of Viburnum erosum

Holzforschung ◽  
2014 ◽  
Vol 68 (7) ◽  
pp. 761-767 ◽  
Author(s):  
Seo-Ji In ◽  
Kyeong-Hwa Seo ◽  
Na-Young Song ◽  
Myoung-Chong Song ◽  
Eun-Mi An ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Column Chromatography ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
Butyl Alcohol ◽  
Chemical Structures ◽  
Etoac Fraction ◽  
C Nmr

Abstract Two new iridoids were isolated and identified to three known ones, namely, loganic acid (2), sweroside (3), and 7-O-tigloylsecologanol (4), from the stems of Viburnum erosum. Dried stems of V. erosum were extracted with 80% methanol (MeOH), and the concentrated extract was partitioned successively with ethyl acetate (EtOAc), n-butyl alcohol (n-BuOH), and H2O. Five compounds were isolated after repeated silica gel (SiO2), octadecyl SiO2 (ODS), and Sephadex LH-20 column chromatography of the EtOAc fraction. The chemical structures of the compounds were determined based on spectroscopic data from 1H- and 13C-NMR, DEPT, and 2D-NMR (gCOSY, gHSQC, gHMBC) data. The new iridoids were identified as 3,7-dihydroxy-8-methyl-cyclopenta[c]pyran-4-carboxylic acid (1) and rel-(1S,5R,9S)-9-ethenyl-1-(β-D-glucopyrinosyloxy)-5,9-dihydro-5-{2-[(2-methylbut-2-enoyl)oxy]ethyl}-1H-pyran-4-carboxylic acid (5) and named viburnin and epi-7-O-tigloylsecologanolic acid, respectively. This is the first report of the isolation of these five iridoids from V. erosum.

scholarly journals Design and synthesis of a novel 5-(aminomethylene)thiazolidine-2,4-dione derivatives as potent hepatitis-B virus polymerase inhibitors

2015 ◽  
Vol 10 (2) ◽  
pp. 271
Author(s):  
Wei-Guo Li ◽  
He-Qun Wang
Keyword(s):  
Hbv Dna ◽  
Biologically Active ◽  
Secondary Amines ◽  
Design And Synthesis ◽  
H Nmr ◽  
Viral Antigens ◽  
Polymerase Inhibitors ◽  
Potent Inhibition ◽  
B Virus ◽  
C Nmr

<p>A series of novel thiazolidinedione analogues (TZD) were designed and synthesized potent inhibitors of HBV capsid assembly. The synthesis of thiazolidine-2,4-dione derivatives (4a–4o), starting from the condensation of 5-(ethoxymethylene)thiazolidine-2,4-dione (1) with various secondary amines (3) derived from biologically active compounds. The newly synthesized TZD analogues 4a-4o were characterized by <sup>1</sup>H NMR, <sup>13</sup>C NMR, and MS and evaluated for their anti-HBV activity. Most of the compounds inhibited the expression of viral antigens at low concentration. Six compounds, 4g, 4h, 4l, 4m, 4n, and 4o, demonstrated potent inhibition of HBV DNA replication at submicromolar range. Of these five initial hits, compound 4o was the most active when compared with lamivudine.</p><p> </p><p> </p>

Cardenolide aus Ornithogalum nutans (2 n = 28), 1. Mitteilung / Cardenolides from Ornithogalum nutans (2 n = 28), Part

1992 ◽  
Vol 47 (10) ◽  
pp. 1444-1458 ◽  
Author(s):  
Roland Ferth ◽  
Andreas Baumann ◽  
Wolfgang Robien ◽  
Brigitte Kopp
Keyword(s):  
Acid Hydrolysis ◽  
Column Chromatography ◽  
Structure Elucidation ◽  
European Species ◽  
H Nmr ◽  
First Time ◽  
Hydrolysis Of ◽  
C Nmr

From leaves and bulbs of Ornithogalum nutans L. (2 n = 28), seventeen cardenolides were isolated by column chromatography, DCCC and TLC. The structure elucidation was performed by means of 1H NMR, 13C NMR, HH-Cosy, HC-Cosy and FAB-MS studies and identification of the sugar moieties by GLC after acid hydrolysis of the cardenolides. Sugar compounds were identified as digitoxose, 3-acetyl-digitoxose, 2-deoxy-allose, 6-deoxy-allose, rhamnose, xylose and apiose. Glycosides of 7β,15β, 16 α-trihydroxy-uzarigenin, 8β,16 α-dihy-droxy, 15-oxo-uzarigenin, 3 β, 11β-dihydroxy, 12-oxo, 18-nor-5 α-card-13-enolid, 11 α-hydroxygitoxigenin, 12-oxo,8, 14β-epoxy-uzarigenin, 8β-hydroxy, 15-oxo-uzarigenin and 12β-hydroxy-oleandrigenin are described for the first time, the presence of oleandrigenin-glycosides in the genus Ornithogalum was not known until now. Ornithogalum nutans L. shows a different cardenolide pattern from the second European species of the section Myogalum (LINK) PETERM. - Ornithogalum boucheanum (KUNTH) ASCHERS.

(3E)-5-Hydroxy-3-[5-(hydroxymethyl)furan-2-ylmethylene]-1-benzofuran-2(3H)-one

2007 ◽  
Vol 63 (11) ◽  
pp. o4354-o4354
Author(s):  
Hong-Yan Ma ◽  
Ji Shi ◽  
Chang-Hong Wang ◽  
Zheng-Tao Wang
Keyword(s):  
Hydrogen Bonds ◽  
Spectroscopic Data ◽  
Furan Ring ◽  
Crystallographic Analysis ◽  
Relative Configuration ◽  
X Ray ◽  
H Nmr ◽  
Compound C ◽  
A Chain ◽  
C Nmr

The title compound, C14H10O5, was isolated from Senecio cannabifolius Less var. integrifolius aqueous extract. The structure was elucidated on the basis of spectroscopic data, including MS, 1H NMR and 13C NMR, and the relative configuration was confirmed by X-ray crystallographic analysis. The benzofuran ring is almost coplanar with the furan ring [dihedral angle = 10.47 (15)°]. A chain is formed through supramolecular R 2 2(10) synthons and three-centre hydrogen bonds.

scholarly journals Chemical Constituents of the Insecticidal Active Extract of Tinospora crispa

2019 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Nor Aziyah Bakhari ◽  
Siti Nur Amirah Diana Fadzillah ◽  
Norain Isa
Keyword(s):  
Blood Pressure ◽  
Petroleum Ether ◽  
Silica Gel ◽  
1H Nmr ◽  
Column Chromatography ◽  
Mobile Phase ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
First Time

Tinospora crispa Miers (Menispermaceae) is a climbing vine with stems rich in warts. The plant is called Akar Seruntum or Patawali in Malaysia and is widely used for treating skin complaints, malaria, bacterial abscess, high blood pressure and diabetes. In the present study, the stems of T. crispa were collected from the locality and succesively extracted with petroleum ether, followed by chloroform and ethanol. The insecticidal active extract (ethanol extract) was  subjected to column chromatography of silica gel eluted with a gradient mobile phase containing hexane, chloroform and ethanol. Among the chemical constituents isolated are n-tetracosyl trans-ferulate and n-octacosyl alcohol, along with three known aporphine alkaloids; N-formylnornuciferine, N-acetylnornuciferine and lysicamine. All compounds were identified by comparing their spectroscopic data (UV, IR, 1H NMR, MS) with data from corresponding values in the literature. Isolation of n-tetracosyl trans-ferulate and n-octacosyl alcohol is reported the first time for T. crispa.

Reaction of 3,5-dicyano-2,4,4,6-tetramethyl-1,4-dihydropyridine with nitric acid

1984 ◽  
Vol 49 (11) ◽  
pp. 2620-2627 ◽  
Author(s):  
Antonín Kurfürst ◽  
Josef Kuthan
Keyword(s):  
Nitric Acid ◽  
Column Chromatography ◽  
Title Compound ◽  
Mass Spectra ◽  
Probable Mechanism ◽  
Mechanism Of Formation ◽  
H Nmr ◽  
C Nmr

Reaction of the title compound I with nitric acid in acetic afforded a mixture of products which on column chromatography afforded 5-cyano-4,4,6-trimethyl-2-nitromethylene-3-oxo-1,2,3,4-tetrahydropyridine (II), (Z)-5-(1-acetoxy-2-nitrovinyl)-3,5-dicyano-4,4-dimethyl-2-pyrazoline (III) and 3-acetoxy-3-cyano-4,4,6-trimethyl-5-oxo-2,3,4,5-tetrahydropyridazine (IV). Reaction of I with nitrating mixture in chloroform gave only the compound II. The probable mechanism of formation of compounds II, III and IV, together with their 1H NMR, 13C NMR, IR and mass spectra, is discussed.

Pd(II) and Pt(II) Complexes of 1,2-Bis(pyridin-2-yl)ethane-N,N'

1994 ◽  
Vol 49 (6) ◽  
pp. 844-848 ◽  
Author(s):  
A. McFarlane ◽  
J. R. Lusty ◽  
J. J. Fiol ◽  
A. Terrón ◽  
E. Molins ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Spectroscopic Data ◽  
Low Energy ◽  
X Ray ◽  
H Nmr ◽  
Direct Bond ◽  
Structures And Properties ◽  
C Nmr

X-ray crystal structures and properties of the two Pd(II) and Pt(II) complexes [bpeH2][PdCl4] and [Pt(bpe)Cl2], [bpe = 1,2-bis(pyridin-2-yl)ethane] are described and correlated with the IR and 1H NMR/13C NMR spectroscopic data. In the case of the Pt(II) complex, the 1,2-bis(pyridin-2-yl)ethane is bound to the metal by the heterocycle nitrogen atoms but no direct bond is found in the case of the Pd(II) complex. The ligand exhibits low energy geometries in both compounds: the cis-conformation in the Pt(II) complex, and the transconformation in the Pd(II) complex

Investigation of the reactivity of 4-amino-5-hydrazineyl-4H-1,2, 4-triazole-3-thiol towards some selected carbonyl compounds: synthesis of novel triazolotriazine-, triazolotetrazine-, and triazolopthalazine derivatives

2019 ◽  
Vol 74 (11-12) ◽  
pp. 847-855
Author(s):  
Kamal M. El-Shaieb ◽  
Asmaa H. Mohamed ◽  
Fathy F. Abdel-latif
Keyword(s):  
Mass Spectrometry ◽  
Elemental Analysis ◽  
Carbonyl Compounds ◽  
Spectroscopic Data ◽  
Pyromellitic Dianhydride ◽  
Isatoic Anhydride ◽  
Naphthalic Anhydride ◽  
H Nmr ◽  
Reaction Proceeds ◽  
C Nmr

AbstractThe reactivity of 4-amino-5-hydrazineyl-4H-1,2,4-triazole-3-thiol (1) towards several carbonyl compounds was investigated. We have found that on treatment of 1 with isatoic anhydride (2), 1,8-naphthalic anhydride (4), diphenic anhydride (6), pyromellitic dianhydride (8), and tetrabromophthalic anhydride (10), the reaction proceeds to give triazolotetrazine- and triazolophthalazine derivatives in good yields using simple experimental procedures. The structure of the synthesized compounds was confirmed using different spectroscopic data (1H NMR, 13C NMR, mass spectrometry, and elemental analysis). The mechanism of the obtained products was also discussed.

scholarly journals Characterization of in-vitro antioxidant, cytotoxic, thrombolytic and membrane stabilizing potential of different extracts of Cheilanthes tenuifolia and Stigmasterol isolation from n-hexane extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Ananna Mahfuz ◽  
Fayad Bin Abdus Salam ◽  
Kanij Nahar Deepa ◽  
A. H. M. Nazmul Hasan
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Column Chromatography ◽  
Hexane Fraction ◽  
Chloroform Fraction ◽  
Potential Candidate ◽  
Methanol Fraction ◽  
H Nmr ◽  
Isolation And Characterization

Abstract Background Cheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern could be abundant of bioactive compounds. The present study was designed to investigate its many therapeutic properties and isolation of bioactive compounds from extracts of Cheilanthes tenuifolia. Methods The dried coarse plant powder was extracted with methanol and dried with rotary evaporator. The extract was further partitioned according to the increasing polarity: N-hexane < chloroform < ethyl-acetate < methanol by modified Kupchan method. Then each extract fractions were investigated for their pharmacologic properties. Compounds were isolated from n-hexane fraction through column chromatography, followed by TLC and structure was determined by analysis of sample using 1H-NMR and matched with published phytochemistry report. Results Methanol fraction of Cheilanthes tenuifolia showed highest amount of total phenol content (11.32 ± 0.28 mg/gm GAE) followed by chloroform fraction (9.71 ± 0.31 mg/gm GAE) > N-hexane fraction (6.69 ± 0.67 mg/gm GAE) > ethyl acetate fraction (5.36 ± 0.54 mg/gm GAE). The methanol fraction of Cheilanthes tenuifolia possessed highest amount (7.11 ± 0.52 mg/gm QE) of total flavonoid content. Our present study indicates that methanol extract was most potent (IC50 = 9.926 μg/ml) inhibitor of DPPH free radicals. In brine shrimp lethality bio assay, all the extracts showed dose dependent increment of mortality and chloroform extract was found most cytotoxic (LC50 = 34.493 μg/ml) compared to other plant extracts. The chloroform fraction of Cheilanthes tenuifolia was most potent in terms of thrombolytic activity. A compound was isolated (CT-2) using column chromatography followed by TCL and PTLC (35% pet ether in CHCl3) and analyzed by 1H-NMR. The structure of stigmasterol was confirmed by comparing the 1H-NMR data with previously published phytochemistry report. Conclusion Cheilanthes tenuifolia could be a potential candidate for bioactive compounds and further studies on isolation and characterization of its bioactive compounds are highly required.

Sign in / Sign up

Export Citation Format

Share Document