scholarly journals Expression of neurofilament subunits in neurons of the central and peripheral nervous system: an immunohistochemical study with monoclonal antibodies

1986 ◽  
Vol 6 (3) ◽  
pp. 650-660 ◽  
Author(s):  
JQ Trojanowski ◽  
N Walkenstein ◽  
VM Lee
Keyword(s):  
Nervous System ◽  
Monoclonal Antibodies ◽  
Peripheral Nervous System ◽  
Immunohistochemical Study

Analysis of glia cell differentiation in the developing chick peripheral nervous system: sensory and sympathetic satellite cells express different cell surface antigens

Development ◽  
1992 ◽  
Vol 115 (2) ◽  
pp. 519-526
Author(s):  
C. Rudel ◽  
H. Rohrer
Keyword(s):  
Nervous System ◽  
Monoclonal Antibodies ◽  
Peripheral Nervous System ◽  
Glial Cells ◽  
Satellite Cells ◽  
Neuronal Cells ◽  
Surface Antigens ◽  
Sympathetic Ganglia ◽  
Precursor Cells ◽  
Gli 1

To identify and analyse precursor cells of neuronal and glial cell lineages during the early development of the chick peripheral nervous system, monoclonal antibodies were raised against a population of undifferentiated cells of E6 dorsal root ganglia (DRG). Non-neuronal cells of E6 DRG express surface antigens that are recognized by four monoclonal antibodies, G1, G2, GLI 1 and GLI 2. The proportion of non-neuronal cells in DRG that express the GLI 1 antigen is very high during ganglion formation (80% at E4) and decreases during later development (15% at E14). GLI 2 antigen is expressed only on a minority of the cells at E6 and increases with development. The G1 and G2 antigens are expressed on about 60–80% of the cells between E6 and E14. All cells that express the established glia marker O4 are also positive for the new antigens. In addition, it was demonstrated that GLI 1-positive cells from early DRG, which are devoid of O4 antigen, could be induced in vitro to express the O4 antigen. Thus, the antigen-positive cells are considered as glial cells or glial precursor cells. Surprisingly, the antigen expression by satellite cells of peripheral ganglia is dependent on the type of ganglion: antigens G1, G2 and GLI 1 were not detectable on glial cells of lumbosacral sympathetic ganglia and GLI 2 was expressed only by a small subpopulation. These results demonstrate an early immunological difference between satellite cells of sensory DRG and sympathetic ganglia.(ABSTRACT TRUNCATED AT 250 WORDS)

Torpedo monoclonal antibodies react with components of the human peripheral nervous system

1988 ◽  
Vol 11 (1) ◽  
pp. 10-20 ◽  
Author(s):  
Elizabeth K. Bjornskov ◽  
Diane T. Stephenson ◽  
Pinky Drosten Kushner
Keyword(s):  
Nervous System ◽  
Monoclonal Antibodies ◽  
Peripheral Nervous System

scholarly journals J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo

Development ◽  
1989 ◽  
Vol 107 (2) ◽  
pp. 309-319 ◽  
Author(s):  
C.D. Stern ◽  
W.E. Norris ◽  
M. Bronner-Fraser ◽  
G.J. Carlson ◽  
A. Faissner ◽  
...  
Keyword(s):  
Nervous System ◽  
Monoclonal Antibodies ◽  
Chick Embryo ◽  
Neural Crest ◽  
Peripheral Nervous System ◽  
Polyacrylamide Gel Electrophoresis ◽  
Neural Crest Cells ◽  
Motor Axons ◽  
Characteristic Set ◽  
Substrate Adhesion

It has been suggested that substrate adhesion molecules of the tenascin family may be responsible for the segmented outgrowth of motor axons and neural crest cells during formation of the peripheral nervous system. We have used two monoclonal antibodies (M1B4 and 578) and an antiserum [KAF9(1)] to study the expression of J1/tenascin-related molecules within the somites of the chick embryo. Neural crest cells were identified with monoclonal antibodies HNK-1 and 20B4. Young somites are surrounded by J1/tenascin immunoreactive material, while old sclerotomes are immunoreactive predominantly in their rostral halves, as described by other authors (Tan et al. 1987—Proc. natn. Acad. Sci. U.S.A. 84, 7977; Mackie et al. 1988—Development 102, 237). At intermediate stages of development, however, immunoreactivity is found mainly in the caudal half of each sclerotome. After ablation of the neural crest, the pattern of immunoreactivity is no longer localised to the rostral halves of the older, neural-crest-free sclerotomes. SDS-polyacrylamide gel electrophoresis of affinity-purified somite tissue, extracted using M1B4 antibody, shows a characteristic set of bands, including one of about 230 × 10(3), as described for cytotactin, J1-200/220 and the monomeric form of tenascin. Affinity-purified somite material obtained from neural-crest-ablated somites reveals some of the bands seen in older control embryos, but the high molecular weight components (120–230 × 10(3] are missing. Young epithelial somites also lack the higher molecular mass components. The neural crest may therefore participate in the expression of J1/tenascin-related molecules in the chick embryo. These results suggest that these molecules are not directly responsible for the segmented outgrowth of precursors of the peripheral nervous system.

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