scholarly journals Impact of Whole Blood Storage Conditions in Flow Cytometric Analysis for Paroxysmal Nocturnal Hemoglobinuria

2021 ◽  
Vol 43 (3) ◽  
pp. 143-151
Author(s):  
Sewon Yoon ◽  
Jimyung Kim ◽  
Seon Young Kim ◽  
Jinsook Lim ◽  
Gye Cheol Kwon ◽  
...  
Keyword(s):  
Whole Blood ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Flow Cytometric Analysis ◽  
Storage Conditions ◽  
Blood Storage ◽  
Flow Cytometric

scholarly journals Whole-Blood Flow-Cytometric Analysis of Antigen-Specific CD4 T-Cell Cytokine Profiles Distinguishes Active Tuberculosis from Non-Active States

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17813 ◽  
Author(s):  
Urban Sester ◽  
Mathias Fousse ◽  
Jan Dirks ◽  
Ulrich Mack ◽  
Antje Prasse ◽  
...  
Keyword(s):  
Blood Flow ◽  
T Cell ◽  
Whole Blood ◽  
Flow Cytometric Analysis ◽  
Active Tuberculosis ◽  
Cd4 T Cell ◽  
Cytokine Profiles ◽  
Flow Cytometric

Clinical Course and Flow Cytometric Analysis of Paroxysmal Nocturnal Hemoglobinuria in the United States and Japan

Medicine ◽  
2004 ◽  
Vol 83 (3) ◽  
pp. 193-207 ◽  
Author(s):  
Jun-Ichi Nishimura ◽  
Yuzuru Kanakura ◽  
Russell E. Ware ◽  
Tsutomu Shichishima ◽  
Hideki Nakakuma ◽  
...  
Keyword(s):  
United States ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Clinical Course ◽  
Flow Cytometric Analysis ◽  
The United States ◽  
Flow Cytometric

Paroxysmal Nocturnal Hemoglobinuria Assessment by Flow Cytometric Analysis

2017 ◽  
Vol 37 (4) ◽  
pp. 855-867 ◽  
Author(s):  
Mike Keeney ◽  
Andrea Illingworth ◽  
D. Robert Sutherland
Keyword(s):  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric

scholarly journals Discordant and heterogeneous expression of GPI-anchored membrane proteins on leukemic cells in a patient with paroxysmal nocturnal hemoglobinuria

Blood ◽  
1993 ◽  
Vol 81 (7) ◽  
pp. 1855-1862 ◽  
Author(s):  
T Shichishima ◽  
T Terasawa ◽  
C Hashimoto ◽  
H Ohto ◽  
M Takahashi ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Mononuclear Cells ◽  
Flow Cytometric Analysis ◽  
Membrane Attack Complex ◽  
Leukemic Cells ◽  
Acute Myelocytic Leukemia ◽  
Lymphocyte Function ◽  
Color Flow ◽  
Flow Cytometric

We performed a flow cytometric analysis using monoclonal antibodies to decay accelerating factor (DAF) and CD59/membrane attack complex inhibitory factor (CD59/MACIF) in order to investigate the leukemic cells and erythrocytes from a patient with paroxysmal nocturnal hemoglobinuria (PNH) who developed acute myelocytic leukemia. In May 1990, the leukemic cells comprised 70% of the mononuclear cells in the bone marrow and 76% of those in the peripheral blood. They consisted of a mixture of positive and negative populations, including single DAF- positive cells. In August 1990, almost 100% of the peripheral mononuclear cells were leukemic blasts, and these consisted of a single population with reduced DAF expression. Single-color flow cytometric analysis showed that the leukemic cells lacked CD59/MACIF, while control leukemic cells (n = 3) expressed both DAF and CD59/MACIF. Leukemic blasts from this patient and six control patients expressed lymphocyte function-associated antigen 3 and FcIII receptors (CD 16) both before and after treatment with phosphatidylinositol-specific phospholipase C. The patient's erythrocytes lacking DAF and CD59/MACIF expression corresponded to the proportion of complement-sensitive cells at the onset of acute leukemia. These DAF- and CD59/MACIF-deficient erythrocytes disappeared almost completely with progression of the leukemia. In conclusion, it appears that the expression of glycosylphosphatidylinositol-linked membrane proteins by leukemic cells was heterogeneous and discordant in our patient, and that the leukemic cells were derived from the PNH clone because of their deficiency of CD59/MACIF. It is also suggested that DAF could compete more effectively than CD59/MACIF for a limited number of anchor molecules available on the proliferating leukemic cells.

scholarly journals Paroxysmal Nocturnal Hemoglobinuria: An Underestimated Cause of Pediatric Thromboembolism

TH Open ◽  
2020 ◽  
Vol 04 (01) ◽  
pp. e36-e39
Author(s):  
Christina Griesser ◽  
Michael Myskiw ◽  
Werner Streif
Keyword(s):  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Clinical Symptoms ◽  
Bone Marrow Failure ◽  
Flow Cytometric Analysis ◽  
Routine Diagnostics ◽  
Glycosyl Phosphatidylinositol ◽  
Vein Thrombosis ◽  
Flow Cytometric ◽  
Appropriate Treatment

AbstractParoxysmal nocturnal hemoglobinuria (PNH) is a chronic disease caused by complement-mediated hemolysis. Clinical symptoms include intravascular hemolysis, nocturnal hemoglobinuria, thromboses, cytopenia, fatigue, abdominal pain, and a strong tendency toward bone marrow failure. It is a rare disease, especially in children, with high mortality rates without appropriate treatment.We here present the case of a 17-year-old girl with unprovoked muscle vein thrombosis. Flow cytometric analysis showed deficiency of glycosyl-phosphatidylinositol-anchored membrane proteins on all three hematopoietic cell lines and confirmed the diagnosis of PNH. Treatment with the monoclonal antibody eculizumab achieved long-term remission.As flow cytometry is normally not part of the routine diagnostics for pediatric thrombosis, awareness is crucial and PNH is important to consider in all children with thrombosis at atypical sites and abnormalities in blood counts with regard to hemolysis and cytopenia.

Sign in / Sign up

Export Citation Format

Share Document