THE ISO-POLAR METABOLITES PRODUCED BY INCUBATION OF [4-14C]TESTOSTERONE WITH RAT AND BOVINE BRAIN

1970 ◽  
Vol 63 (3) ◽  
pp. 512-518 ◽  
Author(s):  
Leon J. Sholiton ◽  
Ila L. Hall ◽  
Emile E. Werk
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Rat Brain ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
Gas Liquid Chromatography ◽  
Polar Metabolite ◽  
Polar Metabolites ◽  
Layer Chromatography

ABSTRACT Rat and bovine brain homogenates have been incubated with [4-14C] testosterone under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less polar, iso-polar, and more polar fractions. The components of the iso-polar fraction were separated after acetylation by thin-layer chromatography and the major end-product identified by gas-liquid chromatography and recrystallization to constant specific activity. Rat brain forms a major iso-polar metabolite, allo-tetrahydrotestosterone, and a small amount of tetrahydrotestosterone. Neither of these could be detected in the bovine brain incubates.

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

UPTAKE OF INTRAVENOUSLY ADMINISTERED PROGESTERONE, PREGNANEDIONE AND PREGNANOLONE BY THE RAT BRAIN

1968 ◽  
Vol 57 (3) ◽  
pp. 395-404 ◽  
Author(s):  
K. H. Raisinghani ◽  
R. I. Dorfman ◽  
E. Forchielli ◽  
L. Gyermek ◽  
G. Genther
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Rat Brain ◽  
Brain Tissue ◽  
Gas Liquid Chromatography ◽  
Radioactive Tracers ◽  
Tissue Extracts ◽  
Layer Chromatography ◽  
The Brain ◽  
Entire Procedure

ABSTRACT A method has been developed for the detection, isolation and quantitation of progesterone, pregnanolone and pregnanedione in brain tissue of rats receiving pharmacological hypnotic doses of these three substances. Prior to extraction radioactive tracers were added to brain tissue obtained from animals receiving these agents and the brain tissue extracts were purified by paper and thin layer chromatography. The material administered as well as some of the major metabolites were quantitated by gas liquid chromatography, and corrections for losses throughout the entire procedure and up to injection into GLC were based on recovery of added radioactive markers. The possible significance of the conversion of progesterone into hypnotically more potent metabolites is discussed.

ANALYSIS OF CHLORORGANIC COMPOUNDS IN FODDER FOR BROILERCHICKENS BY THE METHOD OF GAS-LIQUID CHROMATOGRAPHY WITH MASSSPECTROMETER DETECTOR

2020 ◽  
Vol 1 (2) ◽  
pp. 205-208
Author(s):  
A.A. Parshutina ◽  
◽  
A.A. Solovyova ◽  
L.P. Satyukova ◽  
E.G. Shubina ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Organochlorine Pesticides ◽  
Mass Spectrometer ◽  
Broiler Chickens ◽  
Gas Liquid Chromatography ◽  
Layer Chromatography

The article shows the importance of the study of feed for broiler chickens on the content of organochlorine pesticides. These substances in significant concentrations can disrupt the development of birds and cause mass poisoning. The study of formula feed for broiler chickens «prestart» and «start» by two methodswas conducted: a certified method for the determination of organochlorine pesticides in feed and formula feed (thin layer chromatography) and a method for detecting pesticides not certified for feed (gas-liquid chromatography with mass spectrometer detector). During the experiment, the presence of organochlorine pesticides in several formulafeed samples was revealed.

Additional Cleanup of Samples on GLC Column for TLC Determination of Pesticide Residues

1967 ◽  
Vol 50 (3) ◽  
pp. 615-623
Author(s):  
Kenneth T Hartman
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pesticide Residues ◽  
Gas Liquid Chromatography ◽  
Rf Values ◽  
Layer Chromatography

Abstract Gas-liquid chromatography (GLC) following conventional isolation procedures has been used to clean up pesticide residues for confirmation by thin layer chromatography (TLC). This procedure is more rapid and efficient than present cleanup procedures and permits the determination of pesticide residues that do not survive these rigorous acid or alkali treatments. The method also permits TLC confirmation of pesticide residues that have similar Rf values but different GLC retention times. Recoveries ranged from 85 to 105% for 25 of 28 pesticides tested

Gas-Liquid Chromatography and Liquid Chromatography of Ethylenethiourea in Fresh Vegetable Crops, Fruits, Milk, and Cooked Foods

1977 ◽  
Vol 60 (5) ◽  
pp. 1105-1110 ◽  
Author(s):  
John H Onley ◽  
Laura Giuffrida ◽  
N Fred Ives ◽  
Randall R Watts ◽  
Robert W Storherr
Keyword(s):  
Liquid Chromatography ◽  
Aluminum Oxide ◽  
Thin Layer ◽  
Gas Liquid Chromatography ◽  
Vegetable Crops ◽  
Photometric Detection ◽  
Fresh Vegetable ◽  
Flame Photometric Detection ◽  
Aluminum Oxide Column ◽  
Layer Chromatography

Abstract The Onley-Yip procedure for determining ethylenethiourea (ETU) in milk and crops was modified to reduce interferences by the ethylenebisdithiocarbamates (EBDCs). A 20 g cropmethanol extract is cleaned up by adsorbing the sample onto Gas-Chrom S, desorbing ETU, and eluting ETU from aluminum oxide with chloroform containing ethanol. ETU is converted to the S-butyl derivative for gas-liquid chromatography (GLC) and flame photometric detection (sulfur mode). For liquid chromatography (LC), ETU is cleaned up on another aluminum oxide column and injected directly. LC and GLC results are confirmed by thin layer chromatography. A cooking procedure based on conversion of EBDCs to ETU is included for surveying crops for possible EBDC content. Recoveries from 8 crops and milk fortified at 0.05 ppm ETU ranged from 73 to 100%.

Thin Layer and Gas-Liquid Chromatography of Cholesterol in Fats and Oils. I. Development of Method

1969 ◽  
Vol 52 (4) ◽  
pp. 774-778
Author(s):  
C W Thorpe ◽  
Linda Pohland ◽  
D Firestone
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Unsaponifiable Matter ◽  
Fats And Oils ◽  
Cholesterol Content ◽  
Gas Liquid Chromatography ◽  
Animal Fat ◽  
Low Levels ◽  
Layer Chromatography

Abstract A inethod is described for analysis of sterols by thin layer chromatography-gas liquid chromatography (TLC-GLC). Sterols are isolated from other components of unsaponifiable matter by preparative TLC. The sterols are quantitatively removed from the TLC plate, extracted from the silica gel, and analyzed by GLC. This method has been used to detect low levels (2–3%) of animal fat in vegetable oil by measuring the cholesterol content of the animal fatvegetable oil admixtures.

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