OPTIMUM CULTURE CONDITIONS FOR THE PRODUCTION OF PROLACTIN FROM RAT ANTERIOR PITUITARY ORGAN CULTURES

1970 ◽  
Vol 65 (3) ◽  
pp. 466-476 ◽  
Author(s):  
Richard R. Gala
Keyword(s):  
Stainless Steel ◽  
Salt Solution ◽  
Anterior Pituitary ◽  
Culture Conditions ◽  
The Other ◽  
Organ Cultures ◽  
Hormone Production ◽  
Factors Influencing ◽  
And Function

ABSTRACT Factors influencing prolactin production and anterior pituitary (AP) tissue survival in vitro were investigated. Media 199, NCTC-109, MB752/1 and Trowells-T8 were compared for their ability to support the rat AP in vitro and found to be similar, although Trowells-T8 appeared to be less well suited for this tissue. Culturing AP in Hanks balanced salt solution (BSS) resulted in a lower prolactin production and tissue survival than in Medium 199; nevertheless, there was a tenfold net synthesis of prolactin. Streptomycin and penicillin at a level of 200 units each/ml and nystatin at a level of 50 units/ml were found to control the growth of microorganisms without any deleterious effects on the AP. Amphotercine B at a level of 25 μg/ml, on the other hand, was incompatible with AP tissue survival and function. When pituitary fragments weighed 2.8 mg or more, prolactin production and tissue survival were decreased relative to AP fragments which weighed 1.2 mg or less. Culture of pituitary tissue completely submerged in medium was not compatible with optimum hormone production or AP survival. Pituitary pieces supported by stainless steel platforms with filter paper wicks resulted in better tissue survival and significantly greater prolactin production that when cultured under similar conditions but without a wick.

STUDIES ON THE GROWTH AND HORMONE PRODUCTION OF RAT HYPOPHYSIS IN TISSUE CULTURE

1963 ◽  
Vol 43 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Stig Kullander
Keyword(s):  
Tissue Culture ◽  
Pituitary Gland ◽  
Anterior Pituitary ◽  
Anterior Lobe ◽  
Anterior Pituitary Gland ◽  
The Other ◽  
Pituitary Tumours ◽  
Hormone Production ◽  
Other Hand

ABSTRACT The anterior lobe of the pituitary gland of the rat was studied in tissue culture. Oestrone, progesterone and androsterone did not have any effect on the growth. On the other hand, oestrogen-induced pituitary tumours in tissue culture grew more quickly in medium containing oestrone or androsterone. The anterior pituitary gland produced prolactin in vitro.

scholarly journals Albumin Enhances Caspofungin Activity against Aspergillus Species by Facilitating Drug Delivery to Germinating Hyphae

2015 ◽  
Vol 60 (3) ◽  
pp. 1226-1233 ◽  
Author(s):  
Petros Ioannou ◽  
Aggeliki Andrianaki ◽  
Tonia Akoumianaki ◽  
Irene Kyrmizi ◽  
Nathaniel Albert ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Culture ◽  
Antifungal Agents ◽  
Fold Increase ◽  
Culture Conditions ◽  
The Other ◽  
Related Factors ◽  
Content Type

The modestin vitroactivity of echinocandins againstAspergillusimplies that host-related factors augment the action of these antifungal agentsin vivo. We found that, in contrast to the other antifungal agents (voriconazole, amphotericin B) tested, caspofungin exhibited a profound increase in activity against variousAspergillusspecies under conditions of cell culture growth, as evidenced by a ≥4-fold decrease in minimum effective concentrations (MECs) (P= 0. 0005). Importantly, the enhanced activity of caspofungin againstAspergillusspp. under cell culture conditions was strictly dependent on serum albumin and was not observed with the other two echinocandins, micafungin and anidulafungin. Of interest, fluorescently labeled albumin bound preferentially on the surface of germinatingAspergillushyphae, and this interaction was further enhanced upon treatment with caspofungin. In addition, supplementation of cell culture medium with albumin resulted in a significant, 5-fold increase in association of fluorescently labeled caspofungin withAspergillushyphae (P< 0.0001). Collectively, we found a novel synergistic interaction between albumin and caspofungin, with albumin acting as a potential carrier molecule to facilitate antifungal drug delivery toAspergillushyphae.

Biophysical stimulation for in vitro engineering of functional cardiac tissues

2017 ◽  
Vol 131 (13) ◽  
pp. 1393-1404 ◽  
Author(s):  
Anastasia Korolj ◽  
Erika Yan Wang ◽  
Robert A. Civitarese ◽  
Milica Radisic
Keyword(s):  
Cardiac Tissue ◽  
Culture Media ◽  
Culture Conditions ◽  
Lineage Specification ◽  
Cardiac Tissues ◽  
Cardiac Lineage ◽  
And Function ◽  
Tissue Models

Engineering functional cardiac tissues remains an ongoing significant challenge due to the complexity of the native environment. However, our growing understanding of key parameters of the in vivo cardiac microenvironment and our ability to replicate those parameters in vitro are resulting in the development of increasingly sophisticated models of engineered cardiac tissues (ECT). This review examines some of the most relevant parameters that may be applied in culture leading to higher fidelity cardiac tissue models. These include the biochemical composition of culture media and cardiac lineage specification, co-culture conditions, electrical and mechanical stimulation, and the application of hydrogels, various biomaterials, and scaffolds. The review will also summarize some of the recent functional human tissue models that have been developed for in vivo and in vitro applications. Ultimately, the creation of sophisticated ECT that replicate native structure and function will be instrumental in advancing cell-based therapeutics and in providing advanced models for drug discovery and testing.

scholarly journals Effect of various co-culture systems on embryo development in ovine

2013 ◽  
Vol 58 (No. 10) ◽  
pp. 443-452 ◽  
Author(s):  
B. Heidari ◽  
A. Shirazi ◽  
M.-M. Naderi ◽  
M.-M. Akhondi ◽  
H. Hassanpour ◽  
...  
Keyword(s):  
Embryo Development ◽  
Culture System ◽  
Cell Number ◽  
Culture Conditions ◽  
The Other ◽  
Culture Period ◽  
Hatching Rate ◽  
Embryonic Fibroblast ◽  
One Way Anova

Considering the advent of mesenchymal stem cells (MSCs) as a new source of somatic cells in embryo co-culture system, the current study was aimed to compare in vitro embryo development using embryonic MSCs monolayer with embryonic fibroblast cells (EFCs), oviductal epithelial cells (OECs), and cell-free culture system. The IVM/IVF presumptive sheep zygotes were randomly cultured in different culture conditions as follows: (1) SOFaaBSA medium for the whole culture period (SOF, n = 371), (2) SOFaaBSA medium for the first 3 days followed by co-culturing with MSCs for the next 5 days (SOF-MSCs, n = 120), (3) co-culturing with MSCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (MSCs-SOF, n = 133), (4) co-culturing with MSCs for the whole culture period (MSCs, n = 212), (5) SOFaaBSA medium for the first 3 days followed by co-culturing with EFCs for the next 5 days (SOF-EFCs, n = 132), (6) co-culturing with EFCs for the first 3 days followed by culture in SOFaaBSA medium for the next 5 days (EFCs-SOF, n = 165), (7) co-culturing with EFCs for the whole culture period (EFCs, n = 236), and (8) co-culturing with OECs for the whole culture period (OECs, n = 255). One-Way ANOVA by multiple pairwise comparisons using Tukey&rsquo;s test was performed. Co-culturing in MSCs group had no superiority over EFCs and OECs groups. Though, when co-culturing with MSCs and EFCs was limited to the first 3 days of culture, the embryo development indices were improved compared to the other co-cultured groups. Considering both the hatching rate and total cell number, the application of MSCs for the first 3 days of culture (MSCs-SOF) was superior to the other co-culture and SOF groups. &nbsp;

scholarly journals Vessel-on-a-chip models for studying microvascular physiology, transport, and function in vitro

2020 ◽  
Author(s):  
Savannah R. Moses ◽  
Jonathan J. Adorno ◽  
Andre F. Palmer ◽  
Jonathan W. Song
Keyword(s):  
Cell Culture ◽  
Tissue Level ◽  
Culture Conditions ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Microscale Flow ◽  
Flow Phenomena ◽  
Defined Culture ◽  
And Function

To understand how the microvasculature grows and remodels, researchers require reproducible systems that emulate the function of living tissue. Innovative contributions toward fulfilling this important need have been made by engineered microvessels assembled in vitro using microfabrication techniques. Microfabricated vessels, commonly referred to as "vessels on a chip," are from a class of cell culture technologies that uniquely integrate microscale flow phenomena, tissue-level biomolecular transport, cell-cell interactions, and proper 3-D extracellular matrix environments under well-defined culture conditions. Here, we discuss the enabling attributes of microfabricated vessels that make these models more physiological compared to established cell culture techniques, and the potential of these models for advancing microvascular research. This review highlights the key features of microvascular transport and physiology, critically discusses the strengths and limitations of different microfabrication strategies for studying the microvasculature, and provides a perspective on current challenges and future opportunities for vessel on a chip models.

scholarly journals A comparison of root canal preparations using stainless steel, Ni-Ti hand, and Ni-Ti engine-driven endodontic instruments – an in vitro study

1970 ◽  
Vol 9 (4) ◽  
pp. 223-230
Author(s):  
Krishna Prasad Parvathaneni ◽  
Beena Rani Goel ◽  
Bharani Devi Parvathaneni
Keyword(s):  
Stainless Steel ◽  
Medical Science ◽  
In Vitro Study ◽  
Vitro Study ◽  
The Other ◽  
Preparation Time ◽  
Group I ◽  
Working Length ◽  
Apical Transportation

The main advantage of nickel titanium instruments is that they permit canal preparation with less transportation and ledging. Hand used Ni-Ti and rotary Ni-Ti instruments have a wider range of elastic deformation and greater flexibility. The aim of the present study was to evaluate and compare the preparation time, loss of working length, apical transportation, instrument deformation and fracture with stainless steel, Ni-Ti hand and Ni-Ti rotary endodontic instruments. Fifty freshly extracted human mandibular molars with curved roots were collected and stored in 10% formalin. The samples were divided into 3 groups of 15 each. The access opening was made for each tooth and the biomechanical preparation was carried out using crown down pressureless technique in all the groups. Group I was instrumented with stainless steel files, group II with hand Ni-Ti and group III with Ni-Ti rotary files. The preparation time to enlarge each canal was recorded in minutes and seconds, which included only active instrumentation. Following preparation, the final length of each canal was subtracted from the original length to give the loss of working length. SEM photographs of the deformed and fractured instruments were taken. The apical transportation was measured using computer software (Microdraw 4.1). The readings were noted and statistically analyzed.The results of this in vitro study showed that the mean preparation time was less with Ni- Ti rotary (1.85 min) when compared to hand Ni-Ti (6.33) and stainless steel files (6.73), which was statistically significant. The loss of working length was more for stainless steel group which was statistically significant (P<0.05) when compared with the other 2 groups.One instrument in stainless steel and one in Ni-Ti rotary files were fractured. Only one instrument in stainless steel file deformed permanently. Apical transportation was found to be greater in stainless steel group than other groups (P<0.01) which was statistically significant. Considering the parameters in this study, Ni-Ti rotary files proved to perform better than the other two groups. Key words: Canal preparation; crown-down pressure less technique; double exposure radiographic technique. DOI: 10.3329/bjms.v9i4.6689Bangladesh Journal of Medical Science Vol.09 No.4 July 2010 pp.223-230

Bourgeonnement in vitro a partir d'épiderme séparé de feuille de Bryophyllum Daigremontianum (Crassulacées)

1976 ◽  
Vol 54 (9) ◽  
pp. 852-867 ◽  
Author(s):  
C. Bigot
Keyword(s):  
Solid Medium ◽  
Histological Study ◽  
Culture Conditions ◽  
Epidermal Cells ◽  
The Other ◽  
Naphthaleneacetic Acid ◽  
Lower Epidermis ◽  
Bud Formation

The lower epidermis from leaves of Bryophyllum Daigremontianum Berger has been mechanically separated and cultured in liquid or solid medium; cytokinin and auxin in combination have been necessary to obtain a lamellar callus, with the Murashige and Skoog's macroelements being more efficient than White's, Knop's, or Lin and Staba's. Numerous buds have been initiated after 3 or 4 weeks, especially with naphthaleneacetic acid (1 mg/litre) in combination with 6-benzylaminopurine (0.2 mg/litre). The best results for bud formation have been obtained with the epidermis of the first and second well developed leaves. The buds, rooted in vitro, developed into normal plants. The histological study has showed that the callus was originated only from the banal epidermal cells. The stomatic cells have not been activated and died. The other tissues of the leaf have never produced any bud in the same culture conditions; then, as for other species, the epidermis has a greater ability to initiate a budding programme.

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