THE MEASUREMENT OF HIGH-AFFINITY OESTRADIOL RECEPTORS IN HUMAN UTERINE ENDOMETRIUM AND MYOMETRIUM

1971 ◽  
Vol 68 (3) ◽  
pp. 534-542 ◽  
Author(s):  
D. M. Robertson ◽  
J. Mešter ◽  
J. Beilby ◽  
S. J. Steele ◽  
A. E. Kellie
Keyword(s):  
Menstrual Cycle ◽  
Normal Range ◽  
Biopsy Material ◽  
Uterine Endometrium ◽  
High Affinity ◽  
Biopsy Tissue ◽  
Receptor Sites ◽  
Receptor Concentration ◽  
Human Uterine Endometrium

ABSTRACT The concentration of unoccupied high-affinity oestradiol receptors in the cytosol of human uterine endometrial curettings and biopsy tissue has been determined. In normal specimens, where the day of the menstrual cycle could be assessed histologically, a variation of tissue receptor concentration throughout the cycle was observed showing a maximum at mid-cycle and minima at the beginning and end of the cycle. The distribution of oestradiol receptor sites in the endometrium and myometrium along the length of the uterus has also been studied. Highest concentrations in the endometrium were found in the fundus and these levels fell progressively to negligible concentrations in the isthmus and cervix. In general, the concentration of receptor sites in biopsy material was lower than in curettings and this observation has been related on the region of the uterus from which the samples were obtained. The concentration of receptor sites in abnormal uterine specimens lay within the normal range.

scholarly journals Parametric Images of the Extrastriatal D2 Receptor Density Obtained Using a High-Affinity Ligand (FLB 457) and a Double-Saturation Method

2001 ◽  
Vol 21 (12) ◽  
pp. 1493-1503 ◽  
Author(s):  
Jacques Delforge ◽  
Michel Bottlaender ◽  
Christian Loc'h ◽  
Frédéric Dolle ◽  
André Syrota
Keyword(s):  
Dopamine Receptors ◽  
Quantitative Estimation ◽  
Temporal Cortex ◽  
D2 Receptor ◽  
Experimental Protocol ◽  
D2 Dopamine Receptors ◽  
High Affinity ◽  
Receptor Sites ◽  
Receptor Concentration ◽  
Saturation Method

The potential of positron emission tomography for the quantitative estimation of receptor concentration in extrastriatal regions has been limited in the past because of the low density of the D2 receptor sites in these regions and the insufficient affinity of the most widely used radioligands for dopamine receptors. The new method described in this paper permits the estimate of the D2 receptor concentration in the extrastriatal regions using a two-injection protocol and FLB 457, a ligand with a high affinity (20 pmol/L in vitro) with D2 dopamine receptors. This approach is not valid for the striatal regions because some hypotheses cannot be verified (because of the high receptor concentration in these regions). The experimental protocol includes two injections with ligand doses designed to significantly occupy the extrastriatal receptor sites (≈ 90%), while leaving less than 60% of the receptor sites occupied by the ligand in the striatal regions. The results obtained using this double-saturation method are in line with the concentration estimates previously obtained using the multiinjection approach. The receptor concentration is 2.9 ± 0.5 pmol/mL in the thalamus, 1.0 ± 0.2 pmol/mL in the temporal cortex, and 0.35 ± 0.13 pmol/mL in the occipital cortex. This study provides new arguments supporting the presence of a small receptor-site concentration in the cerebellum, estimated at 0.35 ± 0.16 pmol/mL The simplicity of the calculation used to estimate the receptor concentration lends itself easily to parametric imaging. The receptor concentration is estimated pixel by pixel, without filtering. This method permits estimation of the extrastriatal D2 receptor concentration using an experimental protocol that can easily be used in patient studies (i.e., single experiment, no blood sampling, short experiment duration).

OESTRADIOL RECEPTOR LEVELS IN THE HUMAN FALLOPIAN TUBE DURING THE MENSTRUAL CYCLE

1975 ◽  
Vol 80 (4) ◽  
pp. 705-718 ◽  
Author(s):  
D. M. Robertson ◽  
B.-M. Landgren ◽  
R. Guerrero
Keyword(s):  
Menstrual Cycle ◽  
Fallopian Tube ◽  
Inverse Relationship ◽  
Fallopian Tubes ◽  
Secretory Phase ◽  
Proliferative Phase ◽  
High Affinity ◽  
Human Fallopian Tube ◽  
Receptor Sites ◽  
Nuclear Levels

ABSTRACT The levels of cytoplasmic high-affinity oestradiol receptor sites were determined in the isthmus, isthmus-ampulla, ampulla and fimbria of human Fallopian tubes obtained from 23 women during the proliferative and secretory phase of the menstrual cycle and after menopause. Each segment was homogenized in 0.25 m sucrose and fractionated by differential centrifugation into an 800 g × 15 min nuclear pellet and a 100 000 g×1 h cytoplasmic supernatant and pellet. The nuclear and cytoplasmic pellets were extracted with 0.01 m Tris/HCl pH 8.0 containing 0.25 m sucrose, 1 mM EDTA and 0.1 m KCl. The receptor levels were determined in the 100 000 g × 1 h supernatant (free receptors) and in the KCI extracts (bound receptors). Values are expressed per μg DNA. The concentration of receptors (total, free and bound) was significantly greater in the ampulla than in the isthmus in both phases of the menstrual cycle (P < 0.001). The concentration of free receptors in the isthmus, isthmus-ampulla and ampulla obtained in the proliferative phase was not significantly different from that of the secretory phase. In the fimbria a significantly higher concentration (P < 0.01) was observed in the proliferative phase. The bound receptors were significantly higher in all regions in the proliferative phase than in the secretory phase (P < 0.01). As a consequence the concentration of total receptors was significantly greater along the tube in the proliferative phase (P < 0.01). The levels of oestradiol in the nuclear pellet of each segment as measured by radioimmunoassay were significantly greater in the isthmic region (P < 0.01) than in other regions of the tube. The inverse relationship between the concentration of oestradiol receptor sites and the nuclear levels of oestradiol along the Fallopian tube is discussed.

scholarly journals Changes in the concentration of high-affinity oestradiol receptors in rat uterine supernatant preparations during the oestrous cycle, pseudopregnancy, pregnancy, maturation and after ovariectomy

1970 ◽  
Vol 120 (4) ◽  
pp. 837-844 ◽  
Author(s):  
Patricia Feherty ◽  
D. M. Robertson ◽  
H. B. Waynforth ◽  
A. E. Kellie
Keyword(s):  
Early Pregnancy ◽  
Similar Pattern ◽  
Quantitative Method ◽  
Oestrous Cycle ◽  
High Concentration ◽  
Physiological Conditions ◽  
High Affinity ◽  
Receptor Sites ◽  
Receptor Concentration ◽  
Cyclic Changes

A quantitative method was used to determine the concentration of high-affinity oestradiol-receptor sites in rat uterine supernatant preparations under various physiological conditions. Cyclic changes in concentration were observed during the oestrous cycle, with a maximum occurring in late dioestrus. The changes followed a similar pattern in endometrium and myometrium, although concentrations were higher in the former. In pseudopregnancy the concentration was initially low, rising to a maximum on the tenth day. In early pregnancy a high concentration of receptor was found to be associated with the developing placenta, but this declined in later stages of pregnancy. After ovariectomy or combined ovariectomy and adrenalectomy the receptor concentration remained at a constant low value that could be increased by treatment with oestradiol. The receptor concentration was considerably higher in immature than in adult uteri.

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