Effect of thyroid function on serum somatomedin activity

1981 ◽  
Vol 96 (4) ◽  
pp. 491-497 ◽  
Author(s):  
Josef Marek ◽  
Marie Schüllerová ◽  
Olga Schreiberová ◽  
Zdeňka Límanová
Keyword(s):  
Thyroid Hormones ◽  
Replacement Therapy ◽  
Primary Hypothyroidism ◽  
Embryonic Chick ◽  
Mean Values ◽  
Before And After ◽  
And Function ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

Abstract. To obtain more information about a possible role of somatomedins in mediating the effects of thyroid hormones on the development and function of epiphyseal cartilage, somatomedin activity, measured on the basis of 35S incorporation in embryonic chick cartilage, was studied in thyrotoxic patients before and after treatment with carbimazole or surgery and in a group of patients with primary hypothyroidism. In 29 hyperthyroid patients mean values of somatomedin were 1.29 ± 0.03 sem and differed significantly (P < 0.01) from normals (1.04 ± 0.03). In 15 carbimazole treated patients and in 5 operated patients with thyrotoxicosis somatomedin levels fell from 1.29 ± 0.04 to 1.03 ± 0.04 (P < 0.01) when the patients became euthyroid. Correspondingly, somatomedin levels in 12 untreated hypothyroid patients (0.82 ± 0.04) were significantly less than in normals (P < 0.01) and increased from 0.77 ± 0.09 to 1.14 ± 0.13 (P < 0.05) in 4 patients on replacement therapy. In conclusion: somatomedin levels rise when thyroid hormones are present in excess, and fall when these are deficient. This suggests that thyroid hormones are important regulators of somatomedin levels.

Urine TRH immunoreactivity in hypothyroid and hyperthyroid patients

1984 ◽  
Vol 105 (4) ◽  
pp. 482-486 ◽  
Author(s):  
Anna-Stina Suhonen ◽  
Juhani Leppäluoto ◽  
Jorma Salmi
Keyword(s):  
Healthy Subjects ◽  
Ammonium Acetate ◽  
Polar Solvent ◽  
Primary Hypothyroidism ◽  
Exchange Chromatography ◽  
Reverse Phase ◽  
Before And After ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients ◽  
Serum Tsh

Abstract. Urine samples from 8 healthy subjects, from 16 patients with primary hypothyroidism and 8 patients with Graves' hyperthyroidism were pre-purified in SP-Sephadex-C-25 cation-exchange-chromatography, subjected to reverse phase high-pressure liquid chromatography (HPLC) with 0.01 M ammonium acetate pH 4 as a polar and propanol as a non-polar solvent with a 1%/min gradient and assayed in our TRH radioimmunoassay. Urine TRH-immunoreactivity levels were measured before and after 3 months of treatment with thyroxine or methimazole. The urine TRH-levels in healthy subjects were 5.5 ± 1.4 ng/l (mean ± sem, n = 8). In the hypothyroid patients, the urine TRH levels were 50.6 ±40 ng/l before and 71.7 ± 45.3 ng/l after 3 months of treatment with thyroxine. These values did not significantly differ from those in healthy subjects. The large variations were due to highly elevated values in 3 patients. In 2 hypothyroid patients with initially high urine TRH values, 67 and 657 ng/l, urine TRH was measured 5 and 18 months later and was found to have decreased to 5 and 11 ng/l. In the hyperthyroid patients, urine TRH levels were 10.3 ± 3.9 ng/l before and 8.9 ± 3.3 ng/l after the treatment with methimazole and did not differ significantly from the levels in healthy subjects. After 3 months of treatment, the hyper- and the hypothyroid patients were euthyroid. Our results show, that, except in 2 hypothyroid patients, there does not appear to be any relationship between urine TRH levels and serum TSH or thyroid hormone levels in hypothyroid and hyperthyroid patients.

Lack of effects of circulating thyroid hormone levels on serum leptin concentrations

1997 ◽  
pp. 659-663 ◽  
Author(s):  
S Corbetta ◽  
P Englaro ◽  
S Giambona ◽  
L Persani ◽  
WF Blum ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Standard Deviation Score ◽  
Protein Product ◽  
Primary Hypothyroidism ◽  
Central Hypothyroidism ◽  
Serum Leptin ◽  
Thyroxine Therapy ◽  
Significant Difference ◽  
Before And After ◽  
Thyroxine Replacement Therapy

Leptin is the protein product of the ob gene, secreted by adipocytes. It has been suggested that it may play an important role in regulating appetite and energy expenditure. The aim of this study was to evaluate a possible interaction of thyroid hormones with the leptin system. We studied 114 adult patients (65 females and 49 males): 36 were affected with primary hypothyroidism (PH), 38 with central hypothyroidism (CH) and 40 with thyrotoxicosis (TT). Patients with CH were studied both before and after 6 months of L-thyroxine replacement therapy. Body mass index (BMI; kg/m2), thyroid function and fasting serum leptin were assessed in all patients. Since BMI has been proved to be the major influencing variable of circulating leptin levels, data were expressed as standard deviation score (SDS) calculated from 393 male and 561 female controls matched for age and BMI. No difference in SDS was recorded between males and females whatever the levels of circulating thyroid hormones. In males, no significant difference was recorded among the SDSs of PH (-0.36 +/- 1.2), TT (-0.35 +/- 1.2) and CH (0.01 +/- 1.4) patients. Females with PH had an SDSs significantly lower than TT females (-0.77 +/- 1.0 vs -0.06 +/- 1.2; P < 0.02), while no significant differences between CH (-0.34 +/- 0.7) and TT females or between CH and PH females were observed. SDS in CH patients after 6 months of L-thyroxine therapy significantly varied only in females (0.25 +/- 1.4). In conclusion, circulating thyroid hormones do not appear to play any relevant role in leptin synthesis and secretion. However, as females with either overt hypo- or hyper-thyroidism or central hypothyroidism after L-thyroxine therapy show differences in their SDSs, a subtle interaction between sex steroids and thyroid status in modulating leptin secretion, at least in women, may occur.

STUDY OF GHRELIN LEVELS IN HYPOTHYROID PATIENTS BEFORE AND AFTER TREATMENT

2021 ◽  
pp. 52-54
Author(s):  
Peeyush Yadav ◽  
G. G. Kaushik
Keyword(s):  
Treatment Group ◽  
Thyroid Hormones ◽  
Hdl Cholesterol ◽  
Metabolic Disturbances ◽  
Free T4 ◽  
Before And After ◽  
Group A ◽  
Group B ◽  
Serum Ghrelin ◽  
Hypothyroid Patients

Objective: Aim of the present study was to evaluate the levels of ghrelin in hypothyroid patients before and after treatment with L-thyroxine and to nd a possible relationship between ghrelin and thyroid hormones. Material & Methods: The present study was conducted on 100 hypothyroid patients (44 Males & 56 Females) before treatment (Group A) and after treatment (Group B) attending the outpatient clinics or admitted in wards of J.L.N. Hospitals, Ajmer. 100 healthy control subjects (Group C) of same age group of either gender were selected for the study. Blood samples were drawn from patients and controls, after overnight fast of at least 8 hours. Estimation of Serum Ghrelin, free T3, free T4, and TSH was done by using Enzyme- Linked Immunosorbant Assay (ELISA) technique. Total Cholesterol, Triglyceride, HDL – Cholesterol were measured by automated analyser (Beckman & Coulter's AU680). VLDL – Cholesterol, LDL – Cholesterol were calculated by Friedwald's formula. Differences in the parameters among the groups were analyzed by ANOVA test followed by its Tukey HSD post hoc analysis. Correlations between variables were tested using the Pearson rho (r: Correlation coefcient) correlation test. Results: Findings of the present study shows that the levels of serum fT3 (1.79 ± 0.29 pg/mL) and serum fT4 (0.34 ± 0.11 ng/dL) were signicantly lower in Group A compared to Group B (fT3 = 3.00 ± 0.32 pg/mL & fT4 = 0.81 ± 0.15 ng/dL) and Group C (fT3 = 3.12 ± 0.31 pg/mL & fT4 = 0.85 ± 0.11ng/dL) whereas serum TSH levels were signicantly higher in Group A (40.59 ± 13.55 μIU/mL) compared to Group B (5.34 ± 1.47 μIU/mL) and Group C (3.23 ± 1.04 μIU/mL). Levels of serum Ghrelin were signicantly higher in Group A (918.19 ± 48.47 pg/mL) compared to Group B (700.34 ± 46.35 pg/mL) and Group C (681.49 ± 35.80 pg/mL). A non signicant correlation of Ghrelin with S.fT4 and TSH was found in both Group A and Group B whereas S.fT3 and BMI shows a non signicant correlation in Group A in comparison to a signicant correlation in Group B. Conclusion: There is a reversible increase in the levels of serum ghrelin which became normalized after L-thyroxine substitution in hypothyroid patients. Alteration in the levels of serum ghrelin in thyroid disorders indicates a compensatory role of ghrelin in metabolic disturbances and also suggests a possible association between thyroid hormones and serum ghrelin levels.

scholarly journals Role of reduced thyroid hormone status on bone mineral metabolism

2021 ◽  
Vol 19 (2) ◽  
pp. 26-29
Author(s):  
X Lourdes Sandy ◽  
Keyword(s):  
Thyroid Hormone ◽  
Thyroid Hormones ◽  
Bone Mineral ◽  
Serum Calcium ◽  
Mineral Metabolism ◽  
Bone Mineral Metabolism ◽  
Hormone Status ◽  
Calcium And Phosphorus ◽  
Hypothyroid Patients

Background: The most common endocrine disorder is hypothyroidism which accounts to 11%. Thyroid hormones have a wide array of functions such as physiological growth and development of skeletal system, maintenance of basal metabolic rate and regulation of various metabolisms, including mineral metabolism. Nowadays due to its direct action on bone turn over, thyroid hormones are considered to have an important role on bone mineral metabolism. Thyroid disorders are important cause for secondary osteoporosis. So the present study was done to know the levels of bone minerals, calcium and phosphorus in hypothyroidism and its relation with thyroid hormone levels. Methods: A case-control study was conducted on 30 hypothyroid patients and 30 euthyroid healthy controls in the age group of 20-60 years. Blood samples were collected from all the study population. Serum total triiodothyronine, total thyroxine and TSH by Enzyme-Linked Immunosorbent Assay, Serum calcium by Arsenazo III method, phosphorous by ammonium molybdate method were estimated. Results: Serum calcium levels in cases was found to significantly reduced when compared to controls (p<0.001). Serum phosphorous levels also showed considerable elevation in cases when compared to controls (p<0.001). There was a significant negative correlation between TSH and serum calcium in cases. Conclusion: The present study indicated the important role of reduced thyroid hormone status on bone mineral metabolism. This study concludes that serum calcium was significantly reduced and phosphorus levels were significantly increased in hypothyroid patients when compared to euthyroid control subjects. So frequent monitoring of serum calcium and phosphorus in hypothyroid patients would reduce the burden of bone pathologies.

scholarly journals Thyroid hormones modulate irisin concentrations in patients with recently onset hypothyroidism following total thyroidectomy

2020 ◽  
Author(s):  
R. Bocale ◽  
A. Barini ◽  
A. D‘Amore ◽  
M. Boscherin ◽  
S. Necozione ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Thyroid Hormones ◽  
Total Thyroidectomy ◽  
Replacement Therapy ◽  
Thyroid Function ◽  
Multivariate Regression ◽  
Multivariate Regression Analysis ◽  
Levothyroxine Replacement ◽  
Circulating Levels

Abstract Purpose Irisin is a newly discovered adipo-myokine known for having significant effects on body metabolism. Currently, there is a discussion regarding the relation between thyroid function and irisin concentration. This study was designed to evaluate the influential role of levothyroxine replacement therapy on circulating levels of irisin in patients with recently onset hypothyroidism following total thyroidectomy. Methods Circulating levels of thyroid hormones, irisin and other metabolic parameters, were assessed in 40 recently thyroidectomized patients (34 females, mean age 50.1 ± 15.2 years) at baseline (5–7 day after surgery) and after 2 months under replacement therapy with levothyroxine. Results At baseline, circulating levels of thyroid hormones were indicative of hypothyroidism (TSH 12.7 ± 5.0 μU/mL, FT3 1.9 ± 0.7 pg/mL, FT4 8.7 ± 3.6 pg/mL). Mean serum irisin concentrations significantly increased after 2 months under replacement therapy with levothyroxine (from 2.2 ± 0.6 to 2.9 ± 0.6 μg/mL, p < 0.0001). Variations of circulating levels of irisin under levothyroxine replacement therapy were directly correlated with those of FT3 (Rho = 0.454, p = 0.0033) and FT4 (Rho = 0.451, p = 0.0035). Multivariate regression analysis revealed that changes in thyroid hormones concentrations explained up to 10% of the variations of serum irisin levels under levothyroxine replacement therapy (FT3 R2 = 0.098, FT4 R2 = 0.103). Conclusion Our study suggests that levothyroxine replacement therapy mildly influences irisin metabolism in patients with recently onset hypothyroidism following total thyroidectomy.

scholarly journals Effects of Hypo- and Hyperthyroidism on Noradrenergic Activity and Glycerol Concentrations in Human Subcutaneous Abdominal Adipose Tissue Assessed with Microdialysis

2003 ◽  
Vol 88 (12) ◽  
pp. 5605-5608 ◽  
Author(s):  
Martin Haluzik ◽  
Jara Nedvidkova ◽  
Vladimir Bartak ◽  
Ivana Dostalova ◽  
Petr Vlcek ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Thyroid Hormones ◽  
Relative Magnitude ◽  
In Vivo Microdialysis ◽  
Abdominal Adipose Tissue ◽  
Subcutaneous Abdominal Adipose Tissue ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients ◽  
Tissue Norepinephrine

Abstract Thyroid hormones play a major role in lipid metabolism. However, whether they directly affect lipolysis locally in the adipose tissue remains unknown. Therefore, we measured abdominal sc adipose tissue norepinephrine (NE), basal, and isoprenaline-stimulated lipolysis in 12 hypothyroid patients (HYPO), six hyperthyroid patients (HYPER), and 12 healthy controls by in vivo microdialysis. Adipose tissue NE was decreased in HYPO and increased in HYPER compared with controls (90.4 ± 2.9 and 458.0 ± 69.1 vs. 294.9 ± 19.5 pmol/liter, P &lt; 0.01). Similarly, basal lipolysis, assessed by glycerol assay, was lower in HYPO and higher in HYPER than in controls (88.2 ± 9.9 and 566.0 ± 42.0 vs. 214.3 ± 5.1 μmol/liter P &lt; 0.01). The relative magnitude of isoprenaline-induced glycerol increase was smaller in HYPO (39 ± 19.4%, P &lt; 0.05 vs. basal) and higher in HYPER (277 ± 30.4%, P &lt; 0.01) than in controls (117 ± 5.6%, P &lt; 0.01). The corresponding changes in NE after isoprenaline stimulation were as follows: 120 ± 9.2% (P &lt; 0.05), 503 ± 113% (P &lt; 0.01), and 267 ± 17.2 (P &lt; 0.01). In summary, by affecting local NE levels and adrenergic postreceptor signaling, thyroid hormones may influence the lipolysis rate in the abdominal sc adipose tissue.

THYROID FUNCTION AND PLASMA CYCLIC AMP RESPONSE TO INTRAVENOUS GLUCAGON

1977 ◽  
Vol 85 (4) ◽  
pp. 760-768 ◽  
Author(s):  
S. Nistrup Madsen
Keyword(s):  
Cyclic Amp ◽  
Thyroid Hormones ◽  
Thyroid Function ◽  
Healthy Subjects ◽  
Blood Levels ◽  
Sensitive Test ◽  
Normal Response ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

ABSTRACT The glucagon stimulated increase in plasma cyclic AMP has been studied in 17 healthy subjects, in 13 hyperthyroid and in 14 hypothyroid patients. Six hyperthyroid and 2 hypothyroid patients were re-investigated after at least 15 months of treatment. The results show: 1) The glucagon stimulated cyclic AMP response is significantly increased in hyperthyroid patients considered as a group, and is reduced in patients with hypothyroidism. 2) Three hyperthyroid and 4 hypothyroid patients showed a normal response to iv glucagon, indicating that the plasma cyclic AMP response to iv glucagon is not a sensitive test for the evaluation of peripheral thyroid states. This suggests that the effects of thyroid hormones in the liver does not necessarily follow the effects in other tissues. 3) Re-investigation of treated patients showed that the cyclic AMP response can be normalized by treatment, both in hyperthyroidism and in hypothyroidism. However, in patients treated for hyperthyroidism a hyper-response to glucagon can continue after blood levels of thyroid hormones are reduced to normal. This suggests an inertia in the loss of the hyper-response to glucagon, once a hyperfunction has been induced. A similar inertia in the loss of glucagon sensitivity in hypothyroidism could explain the large number of normal tests in hypothyroid patients.

Intra-individual variation of thyroxin, triiodothyronine, and thyrotropin in treated hypothyroid patients: implications for monitoring replacement therapy.

1988 ◽  
Vol 34 (4) ◽  
pp. 696-699 ◽  
Author(s):  
M C Browning ◽  
W M Bennet ◽  
A J Kirkaldy ◽  
R T Jung
Keyword(s):  
Primary Hypothyroidism ◽  
Free Triiodothyronine ◽  
Post Dose ◽  
Strict Adherence ◽  
Mean Values ◽  
Specimen Collection ◽  
Total Triiodothyronine ◽  
The Mean ◽  
Random Fluctuations ◽  
Hypothyroid Patients

Abstract We measured total thyroxin (TT4), free thyroxin (FT4), total triiodothyronine (TT3), free triiodothyronine (FT3), and thyrotropin (TSH) in serum sampled before and 1, 2, 4, 6, and 8 h after administration of prescribed doses of thyroxin to 12 patients with proven primary hypothyroidism. At 2, 4, and 6 h post-dose, the mean values for TT4 and FT4 and also that at 8 h for FT4 significantly (P less than 0.05) exceeded the corresponding pre-dose values. No significant changes were found for TT3, FT3, or TSH. The mean intra-individual CVs over the study period were TT4 4.9%, FT4 5.7%, TT3 8.7%, FT3 8.7%, and TSH 20.2%. Individual subjects showed small but predictable changes in TT4 and FT4. Changes in TT3 and FT3 were greater but random. Fluctuations in TSH were greatest, but in all subjects with detectable concentrations the variations were of similar magnitude. We conclude that strict adherence to timing of specimen collection in relation to dosage is probably unnecessary.

Lipoprotein(a) and other lipoproteins in hypothyroid patients before and after thyroid replacement therapy

1999 ◽  
Vol 18 (5) ◽  
pp. 319-322 ◽  
Author(s):  
A BECERRA ◽  
D BELLIDO ◽  
A LUENGO ◽  
G PIEDROLA ◽  
D DELUIS
Keyword(s):  
Replacement Therapy ◽  
Lipoprotein A ◽  
Before And After ◽  
Hypothyroid Patients

SEM Observation of Composite Ceramic Scaffolds’ Surface during Incubation in Culture Medium with or without Human PDL Fibroblasts

2011 ◽  
Vol 493-494 ◽  
pp. 866-871
Author(s):  
Anna Theocharidou ◽  
K. Tsoptsias ◽  
Eleana Kontonasaki ◽  
Lambrini Papadopoulou ◽  
C. Panayiotou ◽  
...  
Keyword(s):  
Culture Medium ◽  
Cell Attachment ◽  
Surface Characteristics ◽  
Composite Ceramic ◽  
Surface Porosity ◽  
Periodontal Ligament Fibroblasts ◽  
Eds Analysis ◽  
Before And After ◽  
And Function

Chitin is a polysaccharide abundant in nature. Its’ deacetylation product-chitosan- in combination with gelatin (collagen product) is commonly used asbiopolymer scaffold for tissue engineering. The aim of this study was to investigate diffrerences in surface characteristics of chitin (CHN CCS) and chitosan –gelatin (CHS-G CCS) composite ceramic scaffolds (CCS), during their incubation in culture medium (DMEM) with or without human periodontal ligament fibroblasts (HPDLF). CHN CCS and CHS- G CCS, with pore size 70-200μm, were fabricated on the surface of ceramic disks, being coated with a mixture of bioactive glass – ceramic (1:1 wt). Three CCSs of each type were constructed. Each CCS was incubated at 37 °C up to 10 days, either only in DMEM supplemented with 10% FCS or in DMEM with the presence of 105HPDLF. SEM microphotographs and EDS analysis, before and after incubation, were used to investigate CCSs’ surface alterations. Before incubation, all type of CCSs appeared to be macro porous with high interconnectivity. Exposed to incubation, CHN CCSs’ surface porosity seemed to be rapidly reduced and a rough surface without pores was observed with or without HPDLF. Attached HPDLF were rarely detected. CHS-G CCSs appeared to retain surface porosity in DMEM without cells. In HPDLF culture an almost uniform surface with organic aggregates and attached cells was observed. Until day 10, HPDLF could only be detected at CHS-G CCS’s surface. Conclusion: SEM microphotographs observations indicate that CHN CCSs’ incubation in DMEM led in early and rapid coalescence of surface pores, thus inhibiting HPDLF attachment. HPDLF attachment on CHS-G CCSs confirm the beneficial role of gelatin, while differences in CHS-G CCSs’ surface with and without HPDLF culture indicate that not only sedimentation of medium's ingredients, but cell attachment and function could decrease surface’s porosity, affecting consequently HPDLF proliferation.

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