Stimulation of luteinizing hormone release by epidermal growth factor is mediated by arachidonic acid metabolism in vitro

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S54-S55
Author(s):  
A. PRZYLIPIAK ◽  
L. KIESEL ◽  
T. RABE ◽  
K. HELM ◽  
M. PRZYLIPIAK ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Growth Factor ◽  
Luteinizing Hormone ◽  
Epidermal Growth Factor ◽  
Acid Metabolism ◽  
Arachidonic Acid Metabolism ◽  
Hormone Release ◽  
Epidermal Growth ◽  
Stimulation Of

A role for G-proteins in the epidermal growth factor stimulation of phospholipase A2 in rat kidney mesangial cells

1990 ◽  
Vol 10 (4) ◽  
pp. 353-362 ◽  
Author(s):  
Nashrudeen Hack ◽  
Paula Clayman ◽  
Karl Skorecki
Keyword(s):  
Arachidonic Acid ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Phospholipase A2 ◽  
G Protein ◽  
Mesangial Cells ◽  
Rat Kidney ◽  
Glomerular Mesangial Cells ◽  
Epidermal Growth ◽  
Stimulation Of

We have previously demonstrated phospholipase C (PLC) independent activation of phospholipase A2(PLA2) by epidermal growth factor (EGF) in glomerular mesangial cells in culture. In the current study using glass beads to permeabilize [3H]- or [14C]-arachidonate labelled mesangial cells we demonstrate that guanine nucleotides modulate the EGF-mediated stimulation of arachidonic acid release (75% inhibition with 100 μM GDPβS and 108% augmentation with 100 μM GTPγS). GTPγS alone stimulated both the release of free arachidonic acid and production of diacylglycerol (DAG), while EGF itself neither stimulated DAG nor augmented the DAG response to GTPγS. These findings suggest the intermediacy of a G-protein in PLC-independent stimulation of PLA2 by a growth factor, and provide a model system for determining the relationship between G-protein intermediacy and the intrinsic tyrosine kinase activity of the growth factor receptor.

A mechanism for the in vitro stimulation of adrenal cortisol biosynthesis by epidermal growth factor

1985 ◽  
Vol 17 (8) ◽  
pp. 857-862 ◽  
Author(s):  
Patchanee Singh-Asa ◽  
Michael J. Waters ◽  
Peter A. Wilce
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epidermal Growth ◽  
Stimulation Of

Epidermal growth factor stimulates luteinizing hormone and arachidonic acid release in rat pituitary cells

1988 ◽  
Vol 57 (1-2) ◽  
pp. 157-162 ◽  
Author(s):  
Andrzej Przylipiak ◽  
Ludwig Kiesel ◽  
Thomas Rabe ◽  
Klaus Helm ◽  
Maria Przylipiak ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Growth Factor ◽  
Luteinizing Hormone ◽  
Epidermal Growth Factor ◽  
Pituitary Cells ◽  
Arachidonic Acid Release ◽  
Rat Pituitary ◽  
Epidermal Growth ◽  
Acid Release ◽  
Rat Pituitary Cells

Epidermal growth factor stimulates secretion of rat pituitary luteinizing hormone in vitro

1985 ◽  
Vol 108 (2) ◽  
pp. 175-178 ◽  
Author(s):  
Akira Miyake ◽  
Keiichi Tasaka ◽  
Shirou Otsuka ◽  
Hiroko Kohmura ◽  
Hiroshi Wakimoto ◽  
...  
Keyword(s):  
Growth Factor ◽  
Luteinizing Hormone ◽  
Epidermal Growth Factor ◽  
Female Rats ◽  
Gonadotrophin Secretion ◽  
Significant Release ◽  
Lh Release ◽  
Epidermal Growth ◽  
Lh Releasing Hormone

Abstract. The effects of epidermal growth factor (EGF) on pituitary luteinizing hormone (LH) release and on the releases induced by oestradiol (E2) and LH-releasing hormone (LRH) were examined in a sequential double chamber perifusion system. In this system the mediobasal hypothalami (MBH) and/or pituitaries excised from normally cycling female rats in dioestrus were perifused with test media. Perifusion with EGF at 1 ng/ml for 30 min induced significant release (80–100% increase, P <0.05) of LH from hypothalamo-pituitary pairs, but not from the pituitary alone. Perifusion of the pituitary alone with medium containing 1 ng/ml EGF, resulted in significant release of LH (70–140% increase, P < 0.05) after adminnistration of 10−7 m E2, but did not significantly influence LH release in response to 20 ng/ml LRH. These findings suggest that EGF may be involved in the regulation of pituitary gonadotrophin secretion by a direct effect on the hypothalamus and indirectly by increasing the pituitary responsiveness to E2.

Effect of epidermal growth factor on secondary palatal epithelium in vitro: tissue isolation and recombination studies

Development ◽  
1980 ◽  
Vol 58 (1) ◽  
pp. 93-106
Author(s):  
Mary S. Tyler ◽  
Robert M. Pratt
Keyword(s):  
Cell Death ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Programmed Cell Death ◽  
Dna Synthesis ◽  
Thymidine Incorporation ◽  
Control Medium ◽  
Epidermal Growth ◽  
Stimulation Of

Previous studies have shown that epidermal growth factor (EGF), a peptide of m.w. 6045, can specifically inhibit in organ culture the cessation of DNA synthesis and programmed cell death that normally occur in the presumptive fusion zone (PFZ) of the secondary palatal epithelium. The aim of this study was to determine if EGF acts directly on the epithelium to exert its effect and if there is a requirement for the underlying mesenchyme. Palatal processes from 13- and 14-day Swiss Webster embryonic mice were enzymatically separated into epithelium and mesenchyme which were then cultured alone or in transfilter recombination for up to 72 h. Tissues were examined by transmission- and scanning-electron microscopy and DNA synthesis was monitored autoradiographically using [3H]thymidine incorporation. In isolated epithelium cultured in control medium, cell death occurred in the PFZ and DNA synthesis did not occur in the oral and nasal epithelial regions. EGF (20–50 ng/ml) did not prevent cell death in the PFZ and failed to stimulate DNA synthesis in the isolated epithelium; EGF, however, did have an effect on epithelial cell morphology. In the presence of mesenchyme and EGF, there was extensive proliferation in the entire epithelium and cell death within the PFZ was not evident. The results indicate that the stimulation of DNA synthesis in the palatal epithelium by EGF requires the presence of the underlying mesenchyme and that EFG alone is not sufficient to inhibit programmed cell death within the PFZ of the isolated palatal epithelium.

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