ON THE MECHANISM OF THE IN VITRO UPTAKE OF 131I LABELLED L-TRIIODOTHYRONINE BY HUMAN ERYTHROCYTES

1960 ◽  
Vol XXXIII (I) ◽  
pp. 134-141 ◽  
Author(s):  
Thorkild Friis
Keyword(s):  
Serum Proteins ◽  
Normal Subjects ◽  
Human Erythrocytes ◽  
Hyperthyroid Patients ◽  
In Vitro Uptake

ABSTRACT In an attempt to elucidate the mechanism of the l-triiodothyronine uptake by human erythrocytes 4 sets of experiments were performed: (1) Criss-cross experiments using serum and erythrocytes from hyperthyroid patients and normal subjects, (2) experiments using dilution of the sera, (3) experiments using addition of varying quantities of stable l-triiodothyronine, and (4) addition of varying quantities of stable l-thyroxine. It is demonstrated that the uptake by the erythrocytes is dependent on the binding of triiodothyronine to the serum proteins. Increased binding involves a decreased uptake by the erythrocytes.

IN VITRO UPTAKE OF 131I LABELLED L-TRIIODOTHYRONINE BY HUMAN ERYTHROCYTES

1960 ◽  
Vol XXXIII (I) ◽  
pp. 117-133 ◽  
Author(s):  
Thorkild Friis
Keyword(s):  
Pregnant Women ◽  
Normal Subjects ◽  
Human Erythrocytes ◽  
Homologous Serum ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients ◽  
In Vitro Uptake

ABSTRACT The uptake of 131I labelled l-triiodothyronine by erythrocytes was studied in 139 patients in the presence of equal parts of homologous serum. In 54 normal subjects the uptake was found to range from 6 to 10.5 per cent of the added activity, while 3 subjects (5.6 per cent) showed values from 10.5 to 12.2 per cent. Out of 16 hyperthyroid patients 14 (87.5 per cent) had elevated values. There was a distinct relationship between the severity of thyrotoxicosis and the extent of the uptake. Out of 8 hypothyroid patients 4 (50 per cent) had reduced uptake. Among 12 pregnant women uptake was reduced in 10 and among 8 patients on stilboestrol medication it was reduced in 8. Three out of 7 patients with long-standing hepatitis showed reduced erythrocyte uptake. With one exception the uptake was normal in 13 patients with non-toxic goitre, in 8 euthyroid thyroidectomized patients, in 4 euthyroid patients treated with desiccated thyroid, in 6 euthyroid hypermetabolic and in 3 euthyroid hypometabolic patients.

IN VITRO UPTAKE OF RADIOACTIVE 131I LABELLED L-TRIIODOTHYRONINE BY HUMAN ERYTHROCYTES AS AN INDEX OF THYROID FUNCTION

1960 ◽  
Vol XXXIV (II) ◽  
pp. 305-311 ◽  
Author(s):  
M. G. Woldring ◽  
A. Bakker ◽  
H. Doorenbos
Keyword(s):  
Thyroid Function ◽  
Incubation Time ◽  
Clinical Results ◽  
Human Erythrocytes ◽  
Red Cell ◽  
Clinical Value ◽  
Blood Samples ◽  
In Vitro Uptake

ABSTRACT The red cell triiodothyronine uptake technique as used in our hospital is described. Incubation time is of almost no importance. The temperature during incubation should be 37° C. Further improvement of the technique is obtained when all blood samples are brought up to 40 % haematocrit prior to incubation. Clinical results are discussed. It is yet too early to give a definite assessment of its clinical value, but it is definitely superior to the measurement of the BMR.

ALTERATIONS OF THYROXINE-BINDING PROTEIN IN CLINICALLY HYPO- AND HYPERTHYROID PATIENTS WITH NORMAL PBI LEVEL

1964 ◽  
Vol 45 (1) ◽  
pp. 99-113 ◽  
Author(s):  
Th. Lemarchand-Béraud ◽  
M.-R. Assayah ◽  
A. Vannotti
Keyword(s):  
Thyroid Hormones ◽  
Binding Capacity ◽  
Binding Protein ◽  
Infectious Hepatitis ◽  
Low Level ◽  
Elevated Protein ◽  
Hyperthyroid Patients ◽  
In Vitro Uptake

ABSTRACT Six patients, clinically hypothyroid in spite of a normal or elevated protein-bound iodine (PBI) level, and six hyperthyroid patients with a low level of PBI are described. In these patients, a probable modification of the transport of the thyroid hormones is revealed by means of in vitro uptake of T3 by erythrocytes (Hamolsky's test) and by the determination of the binding capacity of the thyroxine-binding protein (TBP or TBG). In the first group, the labelled triiodothyronine uptake by the erythrocytes is low and the TBP-binding capacity is higher than normal. In the second group, the elevated percentage of free hormones and the low TBP-binding capacity may partly explain the hyperthyroidism despite the low PBI level. PBI concentration in the thyrotoxic range with high TBP binding capacity, is also demonstrated in infectious hepatitis. The meaning of these results is discussed.

THE "EFFECTIVE THYROXINE RATIO" – NEW IN VITRO TEST OF THYROID FUNCTION AND ITS CORRELATION WITH FREE THYROXINE

1974 ◽  
Vol 76 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Janusz Nauman ◽  
Alicja Nauman
Keyword(s):  
Pregnant Women ◽  
Normal Subjects ◽  
Free Thyroxine ◽  
In Vitro Test ◽  
High Reproducibility ◽  
Vitro Test ◽  
The Mean ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

ABSTRACT The effective thyroxine ratio (ETR) and absolute concentration of free thyroxine (AFT4) were estimated in the sera of 31 normal subjects, 27 hyperthyroid patients, 12 hypothyroid patients and 21 euthyroid pregnant women. The mean ETR value in the controls was 1.0 ± 0.18, in the hyperthyroid patients 1.31 ± 0.25, in the hypothyroid patients 0.71 ± 0.21 and in normal pregnant women 0.99 ± 0.24. The mean AFT4 in the normal subjects was 3.0 ± 0.53 ng/100 ml, in the hyperthyroid patients 9.49 ± 2.44 ng/ 100 ml, in the hypothyroid patients 0.58 ± 0.15 ng/100 ml and in the pregnant women 2.84 ± 0.63 ng/100 ml, respectively. High reproducibility of ETR and a significant positive correlation between ETR and AFT4 with r = 0.96 suggest that ETR might be a suitable in vitro test for routine clinical evaluation of the thyrometabolic state.

THE IN VITRO UPTAKE BY HUMAN ERYTHROCYTES OF LABELLED L-TRIIODOTHYRONINE IN CASES OF CHRONIC BRONCHITIS WITH CARBON DIOXIDE RETENTION AND RENAL INSUFFICIENCY

1961 ◽  
Vol 36 (3) ◽  
pp. 335-342 ◽  
Author(s):  
Thorkild Friis ◽  
H. P. Østergaard Kristensen
Keyword(s):  
Carbon Dioxide ◽  
Renal Insufficiency ◽  
Serum Creatinine ◽  
Oxygen Saturation ◽  
Chronic Bronchitis ◽  
Binding Capacity ◽  
Human Erythrocytes ◽  
Arterial Blood ◽  
In Vitro Uptake

ABSTRACT The uptake by human erythrocytes of labelled l-triiodothyronine in the presence of homologous plasma was measured in 25 patients with chronic bronchitis with varying degrees of CO2 retention and in 33 patients with chronic pyelonephritis with varying degrees of uraemia. Increased T3 uptake was found in 20 patients with chronic bronchitis and in 20 patients with uraemia. Cross experiments make it probable that the chief reason for this increase both in patients with bronchitis and with pyelonephritis is due to a reduction in the binding capacity of serum protein for l-triiodothyronine. In the patients with bronchitis increased Pco2 and reduced oxygen saturation in arterial blood were accompanied by increased T3 uptake by the erythrocytes. In the patients with nephritis increasing serum creatinine was accompanied by increased T3 uptake. No relationship could be found between the extent of the acidosis and the T3 uptake.

THE EFFECT OF PREDNISONE ON THE THYROID FUNCTION WITH SPECIAL REFERENCE TO THE IN VITRO UPTAKE BY ERYTHROCYTES OF L-TRIIODOTHYRONINE LABELED WITH 131I

1963 ◽  
Vol 42 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Thorkild Friis ◽  
Vagn Reinicke
Keyword(s):  
Thyroid Hormone ◽  
Thyroid Gland ◽  
Thyroid Function ◽  
Serum Proteins ◽  
Binding Capacity ◽  
Hormone Secretion ◽  
Steroid Treatment ◽  
Secretion Rate ◽  
In Vitro Uptake

ABSTRACT In order to study the effect of steroids on the function of the thyroid gland, the uptake by erythrocytes of l-triiodothyronine labeled with 131I was investigated in 29 euthyroid patients treated with prednisone. At the same time the thyroid function was studied by determinations of the serum PBI, the thyroid 131I-uptake and the thyroid hormone secretion rate. In addition some studies of the renal 131I-clearance were performed. Prednisone was found to increase the erythrocyte uptake of the thyroid hormone triiodothyronine in two-thirds of the patients investigated probably as a result of decreased binding capacity of the serum proteins. Simultaneously, the function of the thyroid gland was found to decrease, according to the thyroid 131I-uptake and the thyroid hormone secretion rate. A slightly increased renal 131I-clearance was found during the steroid treatment. This increase cannot completely explain the decreased thyroid 131I-uptake The relation between the decreased function of the thyroid gland and the increased uptake of triiodothyronine in erythrocytes during steroid treatment is discussed.

OBSERVATIONS OF THE EFFECT OF ORGANIC DYES AND DIPHENYLHYDANTOIN ON THE IN VITRO BINDING OF RADIOACTIVE 131I LABELED L-THYROXINE, L-TRIIODOTHYRONINE AND TETRAIODOTHYROACETIC ACID BY HUMAN ERYTHROCYTES AND SERUM PROTEINS

1965 ◽  
Vol 48 (3) ◽  
pp. 473-480 ◽  
Author(s):  
M. J. Olichney ◽  
K. R. Crispell ◽  
J. D. Harrah
Keyword(s):  
Thyroid Hormones ◽  
Human Serum ◽  
Evans Blue ◽  
Trypan Blue ◽  
Serum Proteins ◽  
Organic Dyes ◽  
Human Erythrocytes ◽  
Blue Dye ◽  
Vitro Binding

ABSTRACT The human erythrocyte is used as a model to study the in vitro binding of thyroid hormones to tissue proteins. Studies on the in vitro binding of 131I labeled 1-thyroxine, 1-triiodothyronine and tetraiodothyroacetic acid to human erythrocytes in buffered saline are reported. The effect of human serum, human serum albumin, the organic dyes, Evans Blue and Trypan Blue and diphenylhydantoin sodium on the binding of these labeled hormones to human erythrocytes has been studied. Evans Blue dye will block the in vitro uptake by the erythrocyte of 1-thyroxine and 1-triiodothyronine but not tetraiodothyroacetic acid. This suggests that the binding of the latter to the erythrocyte is qualitatively different than that of 1-thyroxine or 1-triiodothyronine. Trypan Blue decreased the serum binding of 1-thyroxine and tetraiodothyroacetic acid while 5,5-diphenylhydantoin reduced the serum binding of 1-thyroxine and 1-triiodothyronine. These results are in accord with the binding properties of human serum proteins for thyroid hormones as demonstrated by other techniques.

In Vitro Incorporation of Sodium Acetate-l-C14 into Leukocyte Lipids of Normal and Leukaemic Subjects as a Function of Incubation Time

1962 ◽  
Vol 02 (02) ◽  
pp. 165-172
Author(s):  
C Miras ◽  
G Lewis ◽  
J Mantzos
Keyword(s):  
Sodium Acetate ◽  
Linear Part ◽  
Lipid Synthesis ◽  
Normal Subjects ◽  
Cytostatic Agents ◽  
Time Intervals ◽  
Total Blood ◽  
Effect Of Treatment ◽  
Product Relation

Summary1. Separated leukocytes or total blood from normal subjects, untreated leukaemic patients and from leukaemic patients treated with cytostatic agents were incubated with CH3COONa-l-C14. Radioactivity of mixed lipids was measured at standard time intervals.2. The time incorporation curve observed with leukocytes from treated leukaemic patients showed after an initial linear part, a more rapid levelling off than the curves observed with leukocytes from untreated and normal subjects.3. Therefore, an indirect effect of treatment on leukocyte lipid synthesis seems to be present.4. Phospholipid and neutral lipid synthesis by leukaemic leukocytes was also studied. The results give no evidence that these fractions as a whole have any precursor-product relation.

Sign in / Sign up

Export Citation Format

Share Document