scholarly journals Kinetics of intraoperative fluorescence diagnosis of parathyroid glands

2004 ◽  
pp. 743-747 ◽  
Author(s):  
RL Prosst ◽  
L Schroeter ◽  
J Gahlen
Keyword(s):  
Clinical Application ◽  
Fluorescence Intensity ◽  
Aminolevulinic Acid ◽  
Operation Time ◽  
Parathyroid Glands ◽  
Surrounding Tissue ◽  
Fluorescence Diagnosis ◽  
Design And Methods ◽  
Kinetics Of ◽  
Intraoperative Fluorescence

OBJECTIVE: Identification of parathyroid glands is often a challenge even for experienced surgeons. The feasibility and efficacy of fluorescence diagnosis for localization of parathyroids has already been proven in an experimental setting. In preparation for a clinical application of this technique in patients undergoing surgery for hyperparathyroidism, we evaluated the kinetics of fluorescence diagnosis. DESIGN AND METHODS: Fifty rats were randomized into eight groups with different photosensitization parameters using the photosensitizer aminolevulinic acid (ALA): a peritoneal lavage was performed with either 1.5 or 3.0% ALA solution and the induced photosensitization times varied from 0.5, 1 and 2 to 4 h. Under special fluorescence illumination, D-light, the exposed operative site with thyroid, parathyroid glands and neck muscles was examined. The identified parathyroid glands were studied according to fluorescence intensity by spectrometric measurement and compared with surrounding tissue. RESULTS: Photosensitizer accumulation in parathyroid glands, indirectly measured by spectrometry, was up to 3.2 times higher than in thyroid and 2.6 times higher than in muscle tissue (2 h photosensitization with 3.0% ALA). Using 1.5% ALA, the optimum fluorescence intensity and ratio/contrast was slightly lower (parathyroid-to-thyroid ratio x 3.0, parathyroid-to-muscle ratio x 1.9) but was reached earlier (1 h) and hence considered as the parameter of choice for a clinical application. CONCLUSIONS: In future clinical application, intraoperative fluorescence diagnosis is expected to increase the ease of identification of atypically located or supernumeric glands. In combination with preoperative diagnostics, this may result in reduced operation time and avoidance of persistent hypercalcaemia.

scholarly journals ATCT-21A PROSPECTIVE PHASE II CLINICAL TRIAL OF 5-AMINOLEVULINIC ACID TO CORRELATE INTRAOPERATIVE FLUORESCENCE INTENSITY WITH HISTOLOGIC CELLULARITY

2015 ◽  
Vol 17 (suppl 5) ◽  
pp. v6.1-v6
Author(s):  
Darryl Lau ◽  
Shawn Hervey-Jumper ◽  
Susan Chang ◽  
Annette Molinaro ◽  
Michael McDermott ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Phase Ii ◽  
Fluorescence Intensity ◽  
Aminolevulinic Acid ◽  
Phase Ii Clinical Trial ◽  
Prospective Phase ◽  
Intraoperative Fluorescence

scholarly journals INTRAOPERATIVE FLUORESCENCE DIAGNOSIS OF PERITONEAL DISSEMINATION IN PATIENTS WITH GASTRIC CANCER

2016 ◽  
Vol 5 (3) ◽  
pp. 9-18 ◽  
Author(s):  
E. A. Suleymanov ◽  
A. D. Kaprin ◽  
E. V. Filonenko ◽  
V. M. Homyakov ◽  
N. A. Grishin ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Aminolevulinic Acid ◽  
Peritoneal Dissemination ◽  
Active Agent ◽  
Stage Iii ◽  
Cancer Stage ◽  
Fluorescence Diagnosis ◽  
Tumor Dissemination ◽  
Standard Laparoscopy ◽  
Intraoperative Fluorescence

The study of diagnostic efficiency of intraoperative fluorescence diagnosis (IOFD) of peritoneal dissemination in patients with gastric cancer comparing with standard laparoscopy was performed in P.A. Hertsen MCRI (the branch of NMRRC). The study included 114 patients with verified diagnosis of gastric cancer stage III–IV. For IOFD the domestic medication alasens (the active agent – 5-aminolevulinic acid) was used orally at dose of 30 mg/kg body weight 3 h prior to the study procedure. As the result of fluorescence diagnosis 10 (6.9%) patients had peritoneal tumor dissemination verified morphologically and undetected by standard laporoscopy. The sensitivity of peritoneal revision for standard laparoscopy in patients with gastric cancer stage III–IV was 60.8%, the specificity – 75.8%, the overall accuracy – 69.1%. The sensitivity for IOFD in patients with gastric cancer stage III–IV was 91.1%, the specificity – 93.9%, the overall accuracy – 92.7%. Thus, fluorescence study allowed improving the specificity, the sensitivity and the overall accuracy of diagnostic test. In the trial IOFD had the maximal diagnostic value in patients with visually undetectable microdissemination in «whight» light and with solitary visible peritoneal foci (the average number of additionally detected metastatic lesions in one patient – 1.22 and 1.4, respectively). Moreover, in the case of fluorescence diagnosis the number of detected microfoci of peritoneal dissemination was showed to increase with enlargement of area of tumor invasion to gastric serosa (at average of 2.29 additionally detected lesions in one patient with serosal invasion more than 2 cm2). The obtained results confirm the perspective of use of fluorescence diagnosis for detection of peritoneal dissemination, determination of true tumor extent, staging of the tumor and also for adjustment of following treatment tactics for this group of patients.

Kinetics of protoporphyrin IX formation in rat oral mucosa and skin after application of 5-aminolevulinic acid and its methylester

2004 ◽  
Author(s):  
Stefan Kristiansson ◽  
Asta Juzeniene ◽  
Petras Juzenas ◽  
Vladimir Iani ◽  
Lennart Löfgren ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Kinetics Of

scholarly journals Photodynamic Identification of Human Parathyroid Glands with 5-aminolevulinic Acid

2006 ◽  
Vol 73 (5) ◽  
pp. 246-247 ◽  
Author(s):  
Haruki Akasu ◽  
Takehito Igarashi ◽  
Kumi Tanaka ◽  
Kazuo Shimizu
Keyword(s):  
Aminolevulinic Acid ◽  
Parathyroid Glands

Fluorescent Detection of Rat Parathyroid Glands via 5-Aminolevulinic Acid

2008 ◽  
Vol 118 (6) ◽  
pp. 1014-1018 ◽  
Author(s):  
Scott A. Asher ◽  
Glenn E. Peters ◽  
Stephen F. Pehler ◽  
Kurt Zinn ◽  
J Robert Newman ◽  
...  
Keyword(s):  
Aminolevulinic Acid ◽  
Parathyroid Glands ◽  
Fluorescent Detection

The Influence of 5-Aminolevulinic Acid on Protochlorophyllide and Protochlorophyll Accumulation in Dark-Grown Scenedesmus

1990 ◽  
Vol 45 (1-2) ◽  
pp. 71-73 ◽  
Author(s):  
Kiriakos Kotzabasis ◽  
Horst Senger
Keyword(s):  
Aminolevulinic Acid ◽  
Protein Complexes ◽  
Chlorophyll Biosynthesis ◽  
Scenedesmus Obliquus ◽  
Good Tool ◽  
Future Studies ◽  
Pigment Protein Complexes ◽  
Kinetics Of ◽  
Grown Culture ◽  
In Cells

The intermediate of chlorophyll biosynthesis, 5-aminolevulinic acid (ALA ), is a necessary prerequisite for the formation of protochlorophyllide (PChlide) and protochlorophyll (PChl) in the dark. The application of ALA to a dark-grown culture of the pigment mutant C-2 A′ of Scenedesmus obliquus increased the amount of PChlide 30-fold and the amount of PChl about 10-fold. The rates of ALA-dependent formation of PChlide and PChl reach their maximum values at different concentrations of added ALA . Similarly, the kinetics of PChlide and PChl formation in cells incubated with ALA are different. Cells of Scenedesmus mutant C-2 A′ incubated with various concentrations of ALA for different periods provide a good tool for future studies differentiating between PChlide and PChl metabolism . − The incorporation of Chl deriving from either PChl or PChlide into different pigment protein complexes is discussed.

scholarly journals A tryptophan synchronous and normal fluorescence study on bacteria inactivation mechanism

2019 ◽  
Vol 116 (38) ◽  
pp. 18822-18826 ◽  
Author(s):  
Runze Li ◽  
Dinesh Dhankhar ◽  
Jie Chen ◽  
Thomas C. Cesario ◽  
Peter M. Rentzepis
Keyword(s):  
Fluorescence Intensity ◽  
Protein Unfolding ◽  
Uv Light ◽  
Quantum Yields ◽  
Continuous Irradiation ◽  
Minute Period ◽  
Bacteria Inactivation ◽  
Tryptophan Residues ◽  
Inactivation Mechanism ◽  
Kinetics Of

The UV photodissociation kinetics of tryptophan amino acid, Trp, attached to the membrane of bacteria, Escherichia coli and Bacillus subtilis, have been studied by means of normal and synchronous fluorescence. Our experimental data suggest that the fluorescence intensity of Trp increases during the first minute of irradiation with 250 nm to ∼ 280 nm, 7 mW/cm2 UV light, and subsequently decreases with continuous irradiation. During this short, less than a minute, period of time, 70% of the 107 cell per milliliter bacteria are inactivated. This increase in fluorescence intensity is not observed when tryptophan is in the free state, namely, not attached to a protein, but dissolved in water or saline solution. This increase in fluorescence is attributed to the additional fluorescence of tryptophan molecules formed by protein unfolding, the breakage of the bond that attaches Trp to the bacterial protein membrane, or possibly caused by the irradiation of 2 types of tryptophan residues that photolyze with different quantum yields.

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