Cord blood insulin-like peptide 3 is reduced in idiopathic cryptorchidism and inversely related to free bisphenol A: a marker and/or an actor of foetal exposure to endocrine disruptors?

This study investigated the occurrence of di (2-ethylhexyl) phthalate (DEHP) di-n-butylphthalate (DBP) and bisphenol A (BPA) in river sediment. The samples were collected from three selected points and extracted by sonication, using n-hexane and ethanol. The organic extracts were analyzed by gas chromatography with flame ionization detection (GC/FID). DBP and BPA were not detected. The average concentrations of DEHP range from 0.72 (±0.04) to 27.90 (± 3.05) ng g-1 of sediment. The best solvent for the extractions was n-hexane. However ethanol also shows good extraction yields of DEHP. These results showed an important anthropic contribution for the river contamination by endocrine disruptors.

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The role of maternal diet on offspring hyperinsulinaemia and adiposity after birth: a systematic review of randomised controlled trials

Journal of Developmental Origins of Health and Disease ◽

10.1017/s2040174421000623 ◽

2021 ◽

pp. 1-14

Author(s):

Sylvia North ◽

Catherine Crofts ◽

Christian Thoma ◽

Caryn Zinn

Keyword(s):

Cord Blood ◽

Randomised Controlled Trials ◽

Assessment Tool ◽

Maternal Diet ◽

Specific Effect ◽

Lifestyle Changes ◽

Controlled Trials ◽

C Peptide ◽

Blood Insulin ◽

Randomised Controlled

Abstract In utero diet may be directly related to the risk of fetal hyperinsulinaemia and offspring metabolic health. This review examines the relationship between maternal dietary exposures and sub-clinical fetal hyperinsulinaemia and neonatal adiposity. Articles were identified in MEDLINE, Web of Science, Cochrane Controlled Register of Controlled Trials, Cumulative Index to Nursing and Allied Health Literature, SCOPUS, and SPORTDiscus (September 2019–March 2021) using the preferred reporting items for systematic reviews and meta-analyses guidelines. PROSPERO registration ID CRD42020146453. Studies were selected by two independent reviewers. Randomised controlled trials (RCT) involving a dietary intervention with pregnant women (healthy pregnancy, gestational diabetes mellitus and obesity) and reporting fetal cord-blood insulin, c-peptide, glucose or adiposity estimates were included. One author extracted all information on main study characteristics and outcomes. Risk of bias was assessed using the Cochrane Collaboration’s bias risk assessment tool. A total of 733 articles were identified. Fourteen articles from 11 RCTs (3614 participants) were included. Studies reviewed showed no specific effect of maternal diet on neonatal cord blood insulin, c-peptide or glucose levels. Infants born to mothers who followed a low glycaemic load (GL) had lower skin fold thickness compared to controls. Interventions that provided individualised nutrition counselling to women with obesity or previous infant born > 4 kg were also associated with lower adiposity. The studies reviewed suggest that lifestyle-based dietary interventions to improve glycaemia (low GL) have a protective effect against excess adiposity. Future studies should incorporate multi-modal interventions with dietary counselling to support lifestyle changes throughout gestation and include assessments of maternal insulin resistance at recruitment.

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P–006 Simultaneous determination of bisphenol A and S in the samples of human seminal fluid

Human Reproduction ◽

10.1093/humrep/deab130.005 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

S Fialková ◽

T Král ◽

J Kohoutek ◽

K Franzová ◽

M Ješeta ◽

...

Keyword(s):

Sample Preparation ◽

Bisphenol A ◽

Endocrine Disruptors ◽

Analytical Method ◽

Seminal Fluid ◽

Limit Of Detection ◽

Semen Parameters ◽

Ms Detection ◽

Validation Criteria ◽

Limit Of Quantitation

Abstract Study question Can we quantitatively determine concentrations of endocrine disruptors namely bisphenol A and S in seminal fluid? Summary answer We developed selective analytical method to simultaneously screen for the presence of bisphenol A (BPA) and S (BPS). What is known already The male reproductive system involves processes, which may be influenced by the disruption of the endocrine system by chemicals called endocrine disruptors (EDs). There is a growing evidence that EDs such as bisphenol A and S may be responsible for the decline in male reproductive health. To date, the claimed adverse effects on male fertility are largely based on the results from studies assessing the relationship between urinary BPA and BPS concentration and semen parameters. The best evidence of an adverse effect of BPA and BPS directly on spermatozoa could be provided by measuring bisphenols concentration directly in seminal fluid. Study design, size, duration To selectively and quantitatively analyzed bisphenols in any biological matrix advanced analytical tools and selective sample preparation protocols must be employed. In this study we developed targeted analytical method based on liquid chromatography tandem mass (LC-MS/MS) detection to measure bisphenol A and S in seminal fluid samples obtained from IVF clinic. A total of 140 samples were analysed. Participants/materials, setting, methods BPA and BPS was extracted from 140 seminal fluid samples using solvent extraction followed by preconcentration step. Samples were analyzed on Agilent 6495 Triple Quadrupole (Agilent Technologies, Santa Clara, CA) operating in the ESI-negative mode. Two MS/MS transitions were used for quantitative LC-MS/MS analyses. Chromatographic separation was achieved on Waters™ ACQUITY™ UPLC™BEH C18 (100 × 2.1 mm, 1.7 µm) column using gradient elution with a mixture of 0.1mM ammonium fluoride and methanol as mobile phases. Main results and the role of chance We developed selective sample preparation method for detection of BPA and BPS in seminal fluid followed by LC-MS/MS detection. The method validation was performed based on FDA guidelines. Validation criteria included limit of detection (LOD), limit of quantitation (LOQ), accuracy and precision. Due to the lack of the certified reference material the validation criteria of the method were assessed in pool of spiked seminal samples. The accuracy of the LC-MS/MS method was evaluated as a percent recovery of the amount of target analyte added into the sample. Recovery rates were above 80% for both analytes. LOD was 0.04 ng/mL for BPA and 0.01 ng/mL for BPS. LOQ was 0.14 ng/mL and 0.02 ng/mL for BPS. Measured BPA concentration ranged from 0.04 ng/mL to 1.62 ng/mL. For BPS, the concentration ranged from 0.01 ng/mL to 0.47 ng/mL. BPA and BPS were detected in 64% and 81% of samples, respectively. Interestingly, BPA showed lower detection frequency compared to BPS. These results are consistent with other studies performed on urine samples. Limitations, reasons for caution The limitation of the developed method is the time-consuming sample preparation and analysis cost. Wider implications of the findings: These results document for the first time the presence of BPS in seminal fluid. Knowing the concentration of BPA and BPS in seminal fluid is crucial for mitigating the associated health risks and initiating intervention and prevention strategies. Our future work will evaluate the influence of BPS concentration on spermatozoa. Trial registration number AZV NV18–01–00544; CZ.02.2.69/0.0/0.0/19_074/0012727

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An epigenome-wide analysis of cord blood DNA methylation reveals sex-specific effect of exposure to bisphenol A

Scientific Reports ◽

10.1038/s41598-019-48916-5 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 4

Author(s):

Ryu Miura ◽

Atsuko Araki ◽

Machiko Minatoya ◽

Kunio Miyake ◽

Mei-Lien Chen ◽

...

Keyword(s):

Dna Methylation ◽

Bisphenol A ◽

Cord Blood ◽

Specific Effect

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Maternal insulin resistance, triglycerides and cord blood insulin in relation to post-natal weight trajectories and body composition in the offspring up to 2 years

Diabetic Medicine ◽

10.1111/dme.12298 ◽

2013 ◽

Vol 30 (12) ◽

pp. 1500-1507 ◽

Cited By ~ 16

Author(s):

S. Brunner ◽

D. Schmid ◽

K. Hüttinger ◽

D. Much ◽

E. Heimberg ◽

...

Keyword(s):

Insulin Resistance ◽

Body Composition ◽

Cord Blood ◽

Blood Insulin ◽

Weight Trajectories

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Endocrine disruptors and reproductive health: The case of bisphenol-A

Molecular and Cellular Endocrinology ◽

10.1016/j.mce.2006.04.033 ◽

2006 ◽

Vol 254-255 ◽

pp. 179-186 ◽

Cited By ~ 376

Author(s):

Maricel V. Maffini ◽

Beverly S. Rubin ◽

Carlos Sonnenschein ◽

Ana M. Soto

Keyword(s):

Reproductive Health ◽

Bisphenol A ◽

Endocrine Disruptors

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Dyslipidemia, insulin resistance, and impairment of placental metabolism in the offspring of obese mothers

Journal of Developmental Origins of Health and Disease ◽

10.1017/s2040174420001026 ◽

2020 ◽

pp. 1-10

Author(s):

Matthew Bucher ◽

Kim Ramil C. Montaniel ◽

Leslie Myatt ◽

Susan Weintraub ◽

Hagai Tavori ◽

...

Keyword(s):

Insulin Resistance ◽

Cord Blood ◽

Metabolic Flux ◽

Maternal Obesity ◽

Plasma Lipids ◽

Normal Weight ◽

Dependent Manner ◽

Blood Insulin ◽

Insulin Resistant ◽

Health And Wellbeing

Abstract Obesity is a chronic condition associated with dyslipidemia and insulin resistance. Here, we show that the offspring of obese mothers are dyslipidemic and insulin resistant from the outset. Maternal and cord blood and placental tissues were collected following C-section at term. Patients were grouped as being normal weight (NW, BMI = 18–24.9) or obese (OB, BMI ≥ 30), and separated by fetal sex. We measured plasma lipids, insulin, and glucose in maternal and cord blood. Insulin resistance was quantified using the HOMA-IR. Placental markers of lipid and energy metabolism and relevant metabolites were measured by western blot and metabolomics, respectively. For OB women, total cholesterol was decreased in both maternal and cord blood, while HDL was decreased only in cord blood, independent of sex. In babies born to OB women, cord blood insulin and insulin resistance were increased. Placental protein expression of the energy and lipid metabolism regulators PGC1α, and SIRT3, ERRα, CPT1α, and CPT2 decreased with maternal obesity in a sex-dependent manner (P < 0.05). Metabolomics showed lower levels of acylcarnitines C16:0, C18:2, and C20:4 in OB women’s placentas, suggesting a decrease in β-oxidation. Glutamine, glutamate, alpha-ketoglutarate (αKG), and 2-hydroxyglutarate (2-HG) were increased, and the glutamine-to-glutamate ratio decreased (P < 0.05), in OB placentas, suggesting induction of glutamate into αKG conversion to maintain a normal metabolic flux. Newly-born offspring of obese mothers begin their lives dyslipidemic and insulin resistant. If not inherited genetically, such major metabolic perturbations might be explained by abnormal placental metabolism with potential long-term adverse consequences for the offspring’s health and wellbeing.

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