scholarly journals Interferon-tau promotes luteal endothelial cell survival and inhibits specific luteolytic genes in bovine corpus luteum

Reproduction ◽  
2017 ◽  
Vol 154 (5) ◽  
pp. 559-568 ◽  
Author(s):  
Raghavendra Basavaraja ◽  
Emilia Przygrodzka ◽  
Bartosz Pawlinski ◽  
Zdzislaw Gajewski ◽  
Monika M Kaczmarek ◽  
...  
Keyword(s):  
Life Span ◽  
Cell Survival ◽  
Corpus Luteum ◽  
Luteal Cell ◽  
Interferon Tau ◽  
Interferon Stimulated Genes ◽  
Proliferative Effect ◽  
Domestic Ruminants ◽  
Prostaglandin F2a ◽  
Bovine Corpus Luteum

Interferon-tau (IFNT), a maternal recognition of pregnancy (MRP) signals in domestic ruminants, suppresses the release of luteolytic pulses of uterine prostaglandin F2a (PGF2a), thus extending the corpus luteum (CL) life span. We hypothesized that IFNT also exerts anti-luteolytic actions in bovine CL. To examine the direct effects of IFNT on bovine CL, luteal slices and enriched luteal endothelial cells (LECs) were utilized. We found that recombinant ovine IFNT (roIFNT) markedly elevates interferon-associated genes (STAT1, STAT2 and IRF9) and interferon-stimulated genes (ISGs: MX2, ISG15 and OAS1Y) in both models. Furthermore, IFNT time-dependently induced STAT1 phosphorylation in LECs without affecting total STAT1. roIFNT-stimulated viable LECs numbers and the knockdown of protein inhibitor of activated STAT1 (PIAS1) abolished this effect, suggesting that PIAS1 may mediate the proliferative effect of IFNT. IFNT significantly downregulated luteolytic genes such as TGFB1, thrombospondin-1 (THBS1), endothelin-1 (EDN1) and serpin family E member-1 (SERPINE1) in LECs. However, less robust effects were observed in luteal slices. Moreover, PGF2a alone induced THBS1, SERPINE1 and EDN1 mRNA in CL slices whereas in the presence of IFNT, THBS1 and SERPINE1 stimulation was abolished. Collectively, these results indicate that IFNT acts via STAT1- IRF9-dependent and independent pathways and affects diverse luteal functions. Most interestingly, this study suggests the existence of an anti-luteolytic effect of IFNT in bovine CL, namely, inhibiting key PGF2a-induced luteolytic genes. The proliferative effect of IFNT may constitute an additional mechanism that promotes luteal cell survival, thus, extending the luteal life span during early pregnancy in cows.

scholarly journals Control of Steroidogenesis in Small and Large Bovine Luteal. Cells

1987 ◽  
Vol 40 (3) ◽  
pp. 331 ◽  
Author(s):  
William Hansel ◽  
Hector W Alila ◽  
Joseph P Dowd ◽  
Xiangzhong Yang
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Corpus Luteum ◽  
Luteal Cell ◽  
Lipoxygenase Pathway ◽  
Luteal Cells ◽  
Kinase C ◽  
Bovine Corpus Luteum ◽  
Progesterone Synthesis

Evidence was cited to show that: (1) prostacyclin (PGI2) plays a luteotrophic role in the bovine corpus luteum and that products of the lipoxygenase pathway of arachidonic acid metabolism, especially 5-hydroxyeicosatetraenoic acid play luteolytic roles; (2) oxytocin of luteal cell origin plays a role in development, and possibly in regression, of the bovine corpus luteum; and (3) luteal cells arise from two sources; the characteristic small luteal cells at all stages of the o~strous cycle and pregnancy are of theca cell origin; the large cells are of granulosa cell origin early in the cycle, but a population of theca-derived large cells appears later in the cycle. Results of in vitro studies with total dispersed cells and essentially pure preparations of large and small luteal cells indicate that : (1) the recently described Ca2+ -polyphosphoinositol-protein kinase C second messenger system is involved in progesterone synthesis in the bovine corpus luteum; (2) activation of protein kinase C is stimulatory to progesterone synthesis in the small luteal cells; (3) activation of protein kinase C has no effect on progesterone synthesis in the large luteal cells; and (4) protein kinase C exerts its luteotrophic effect in total cell preparations, in part at least, by stimulating the production of prostacyclin. The protein kinase C system may cause down regulation of LH receptors in the large cells.

scholarly journals Possible role of interferon tau on the bovine corpus luteum and neutrophils during the early pregnancy

Reproduction ◽  
2015 ◽  
Vol 150 (3) ◽  
pp. 217-225 ◽  
Author(s):  
Koumei Shirasuna ◽  
Haruka Matsumoto ◽  
Shuichi Matsuyama ◽  
Koji Kimura ◽  
Heinrich Bollwein ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Neutrophil Migration ◽  
Peripheral Circulation ◽  
Interferon Tau ◽  
Bovine Corpus Luteum ◽  
Activated Neutrophils ◽  
Endocrine Factor ◽  
Uterine Environment ◽  
And Function

When pregnancy is established, interferon tau (IFNT), a well-known pregnancy recognition signal in ruminants, is secreted by embryonic trophoblast cells and acts within the uterus to prepare for pregnancy. IFNT acts as an endocrine factor on the corpus luteum (CL) to induce refractory ability against the luteolytic action of PGF2α. Hypothesising that IFNT may influence not only the uterine environment but also the CL in cows via local or peripheral circulation, we investigated qualitative changes in the CL of pregnant cows during the maternal recognition period (day 16) and the CL of non-pregnant cows. The CL of pregnant animals had a higher number of neutrophils, and the expression of interleukin 8 (IL8) mRNA and its protein was higher as well as compared with the CL of non-pregnant animals. Although IFNT did not affect progesterone (P4) secretion and neutrophil migration directly, it stimulated IL8 mRNA expression on luteal cells (LCs), influencing the neutrophils, resulting in the increased migration of IFNT-activated neutrophils. Moreover, both IFNT-activated neutrophils and IL8 increased P4 secretion from LCs in vitro. Our novel finding was the increase in neutrophils and IL8 within the CL of pregnant cows, suggesting the involvement of IFNT function within the CL toward establishment of pregnancy in cows. The present results suggest that IFNT upregulates neutrophil numbers and function via IL8 on LCs in the CL of early pregnant cows and that both neutrophils and IL8, stimulated by IFNT, are associated with an increase in P4 concentrations during the maternal recognition period in cows.

Localization of a Prostaglandin F2a Receptor in Bovine Corpus luteum Plasma Membranes

1976 ◽  
Vol 61 (2) ◽  
pp. 605-611 ◽  
Author(s):  
William S. POWELL ◽  
Sven HAMMERSTRoM ◽  
Bengt SAMUELSSON
Keyword(s):  
Corpus Luteum ◽  
Plasma Membranes ◽  
Prostaglandin F2a ◽  
Bovine Corpus Luteum

scholarly journals TAMANHO DA AMOSTRA PARA ESTUDO DA PROPORÇÃO VOLUMÉTRICA DOS CONSTITUINTES DO CORPO LÚTEO BOVINO

2002 ◽  
Vol 7 (2) ◽  
Author(s):  
M. M. NEVES ◽  
A. P. MARQUES JR.
Keyword(s):  
Endothelial Cells ◽  
Soybean Oil ◽  
Corpus Luteum ◽  
Cell Types ◽  
Luteal Cell ◽  
Cell Nuclei ◽  
Cell Cytoplasm ◽  
Bovine Corpus Luteum

O objetivo deste estudo foi estabelecer o número de campos histológicos necessários para quantificar os componentes celulares do corpo lúteo bovino. Para os parâmetros citoplasma das células luteínicas, núcleo das células luteínicas e fibroblasto são necessários 30 campos, enquanto que para células endoteliais e pericito são necessários 35, podendo-se padronizar a técnica em 35 campos e 875 pontos por corpo lúteo. Soybean oil replaced by acidulated soapstock in broiler diets Abstract The purpose of this study was to determine the number of fields necessary for the quantification of the cell types of the bovine corpus luteum. A total of 30 fields is necessary for the quantification of the luteal cell cytoplasm, luteal cell nuclei and fibroblast, and 35 fields for the quantification of endothelial cells and pericycles. For the analysis of both parameters is recommended the study of 35 fields and 875 points per corpus luteum.

Role of apoptosis in functional luteolysis in the pregnant rabbit corpus luteum: Evidence of a role for placental-derived factors in promoting luteal cell survival

APOPTOSIS ◽  
2004 ◽  
Vol 9 (6) ◽  
pp. 807-814 ◽  
Author(s):  
A. M. Dharmarajan ◽  
S. B. Goodman ◽  
N. Atiya ◽  
S. P. Parkinson ◽  
R. R. Lareu ◽  
...  
Keyword(s):  
Cell Survival ◽  
Corpus Luteum ◽  
Luteal Cell ◽  
Pregnant Rabbit

scholarly journals Expression and regulation of secreted phosphoprotein 1 in the bovine corpus luteum and effects on T lymphocyte chemotaxis

Reproduction ◽  
2013 ◽  
Vol 146 (6) ◽  
pp. 527-537 ◽  
Author(s):  
Daniel H Poole ◽  
Kalidou Ndiaye ◽  
Joy L Pate
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Cell Function ◽  
Luteal Cell ◽  
Cellular Interactions ◽  
Western Blots ◽  
Luteal Regression ◽  
Luteal Cells ◽  
Bovine Corpus Luteum ◽  
Secreted Phosphoprotein 1

Secreted phosphoprotein 1 (SPP1) in the bovine corpus luteum (CL) regulates cell function during the transitional periods of luteinization and luteal regression. The objectives were to i) characterize SPP1 expression in the CL throughout the estrous cycle, ii) determine factors that regulate SPP1 expression in luteal cells, and iii) examine the role of SPP1 in lymphocyte chemotaxis, proliferation, and function.SPP1mRNA was greater in fully functional (d10) CL and late cycle (d18) CL compared with developing (d4) CL. Additionally,SPP1mRNA increased within 1 h and remained elevated 4 and 8 h following induction of luteolysis with prostaglandin (PG)F2α. Expression of the SPP1 receptor, β3integrin, was not different throughout the estrous cycle but decreased following induction of luteolysis. Expression ofCD44increased during the estrous cycle but did not change during luteal regression. In cultured luteal cells,SPP1mRNA was upregulated by PGF2αand/or tumor necrosis factor α. Western blots revealed the presence of both full-length SPP1 and multiple cleavage products in cultured luteal cells and luteal tissue. Depletion of endogenous SPP1 did not hinder luteal cell-induced lymphocyte proliferation or lymphocyte phenotype but did inhibit lymphocyte migration toward luteal cells. Based on these data, it is concluded that SPP1 is initially activated to establish and maintain cellular interactions between steroidogenic and nonsteroidogenic cells during the development of the CL. Upon induction of luteolysis, SPP1 serves as a signaling molecule to recruit or activate immune cells to facilitate luteal regression and tissue degradation.

scholarly journals Profiles of prostaglandin F2α metabolite in dairy cattle during luteal regression and pregnancy: implications for corpus luteum maintenance†

2019 ◽  
Vol 101 (1) ◽  
pp. 76-90 ◽  
Author(s):  
Megan A Mezera ◽  
Caleb S Hamm ◽  
Caio A Gamarra ◽  
Rodrigo S Gennari ◽  
Alexandre B Prata ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Peak Concentration ◽  
Prostaglandin F2α ◽  
Interferon Tau ◽  
Similar Frequency ◽  
Luteal Regression ◽  
Oxytocin Receptors ◽  
Bovine Corpus Luteum ◽  
Reproductive Losses

Abstract Mechanisms of bovine corpus luteum (CL) maintenance during the second month of pregnancy have not been adequately investigated, despite significant reproductive losses. In the first month, interferon-tau is believed to suppress oxytocin-stimulated prostaglandin F2α (PGF) production, yet there are conflicting reports of circulating PGF metabolite (PGFM). In this study, characterization of PGFM and P4 occurred through continuous bihourly blood sampling in cows undergoing CL regression (day 18–21, n = 5), and during the first (day 18–21, n = 5) and second month (day 47–61; n = 16) of pregnancy. Cattle in the second month were assigned to control (n = 8) or oxytocin treatment (n = 8; three pulses to mimic luteolysis) to evaluate if oxytocin receptors were active. All cows but one (which had elevated PGFM prior to oxytocin treatment) maintained the pregnancy. Basal PGFM concentrations were low (11.6 ± 0.7 pg/mL) in the first month but increased 2.54-fold in the second month. Few (0.26 ± 0.12 pulses/day) PGFM pulses with low peak concentrations (28.8 ± 3.1 pg/mL) were observed during the first month of pregnancy, similar to cows not undergoing regression. However, in the second month, frequency (1.10 ± 0.26 pulses/day) and peak concentration (67.2 ± 5.0 pg/mL) of PGFM pulses increased, displaying similar frequency but lower peak PGFM than seen in regression (1.44 ± 0.14 pulses/day; 134.5 ± 18.9 pg/mL). Oxytocin treatment increased likelihood of PGFM pulses post-treatment and increased peak concentration (89.7 ± 10.1 pg/mL) in cows during the second month. Thus, cows have more PGFM pulses during second than first month of pregnancy, possibly induced by endogenous oxytocin, indicating suppression of PGF production is an important mechanism for CL maintenance during first but not second month of pregnancy.

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