Determination of hemoglobin A1c (HbA1c), alkaline phosphatase (ALP), aspartate aminotransferase (AST), superoxide dismutase (SOD) values and lipid profiles in rats fed with corn syrup and trans oil added diets

Abstract Methodological modifications have been made in our "SMA 12/60 AutoAnalyzer" system, to improve the reproducibility and accuracy of six commonly performed procedures. These include glucose with the glucose oxidase method, albumin with the bromcresol green method, and alkaline phosphatase with the sodium thymolphthalein monophosphate method. We have also modified the composition of the reagents used in the determination of protein, inorganic phosphate, and aspartate aminotransferase. A general approach that is useful for adapting a new method to a multichannel AutoAnalyzer system is discussed.

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Evaluation of Serum Hepatocellular Enzymes in Nigerian with Goitre

Asian Journal of Medical Sciences ◽

10.3126/ajms.v2i2.3870 ◽

2011 ◽

Vol 2 (2) ◽

pp. 79-81

Author(s):

Johnkennedy Nnodim ◽

Emejulu Adamma ◽

Elendu Humphrey Ndubueze

Keyword(s):

Cardiovascular Disease ◽

Alkaline Phosphatase ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Normal Subjects ◽

Medical Sciences ◽

Free T4 ◽

Imo State ◽

And Control

Objective: The determination of serum gammaglutamyl transferase (GGT),alkaline phosphatase(ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in patient with goitre in Owerri, Imo state Nigeria were investigated.Material & Methods: Thirty confirmed patients with goitre age 50 to 70years with the following thyroid index (Total T4 > 140.65±7.28nmol/l , Total T3 > 2.43±0.96nmol/l, Free T4 < 50.24±9.11nmol/l and TSH > 4.12±1.00nmol/l) were selected for the study. Thirty normal subjects free from goiter age 50 to 70 years were used as control. Patients with complications such as cardiovascular disease, hypertension and diabetes were excluded.Results: The level of serum gamaglutamyl transferase in goitre subjects was significantly higher (50.32 ±4.27 iu/l) when compared with control (17.50±3.94 iu/l) at P< 0.05. In the same vein the level of alkaline phosphatase was significantly higher (110.9m/l±12.92 iu/l) when compared with the control (56.3±12.06 iu/l) P<0.05. The levels of AST and ALT in goitre and control were not significant when compared with the control.Conclusion: This observation shows that gammaglutamyl transferase and alkaline phosphatase are frequently in-creased in goitre. Hence, they are possibly thyroid dependent enzymes.Key Words: Gammaglutamyl transferase; alkaline phosphatase; aspartateaminotransferase; alanine aminotransferase; thyroid hormonesDOI: http://dx.doi.org/10.3126/ajms.v2i2.3870Asian Journal of Medical Sciences 2 (2011) 79-81

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Leucas zeylanica (L.) R. Br. protects ethanol and hydrogen peroxide-induced oxidative stress on hepatic tissue of rats

International Current Pharmaceutical Journal ◽

10.3329/icpj.v2i9.16076 ◽

2013 ◽

Vol 2 (9) ◽

pp. 148-151 ◽

Cited By ~ 2

Author(s):

Shahdat Hossain ◽

Mijanur Rahman ◽

Nusrat Fatima ◽

Mozammel Haque ◽

Jahirul Islam

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Alkaline Phosphatase ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Antioxidative Activity ◽

Lipid Peroxide ◽

Pharmaceutical Journal ◽

Hepatic Tissue

The aim of the study was to evaluate the effect of Leucas zeylanica against oxidative stress on hepatic tissue. Oxidative stress was induced by exposing hepatic tissue to ethanol and Fentons reagent (H2O2+FeSO4). The effect of oxidative stress on liver also was evaluated by the determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activity and the levels of lipid peroxide (LPO). The antioxidative activity of L. zeylanica was determined by estimating it ability to inhibit the hepatic levels of lipid peroxide (LPO), as indicator of oxidative stress. Concomitantly, the antioxidant phytochemicals such as polyphenols and flavonoids were assessed against pyrogallol and quercetin standards. The ALT and AST activities and the levels of LPO of hepatic tissue were significantly increased by oxidative stress. L. zeylanica pretreatment, however, significantly repressed the oxidative stress on hepatic tissue, as indicated by the decreased activities of ALT and AST enzymes and levels of LPO. Analyses of the phytochemicals revealed that the extract of L. zeylanica contained substantial amounts of polyphenols (74.32 ± 4.6 µg of pyrogallol equivalent/mg) and flavonoids (15.69 ± 2.2 µg quercetin equivalent/mg of extract). Finally, the results of the present study demonstrated the presence of antioxidant phytochemicals, including polyphenols and flavonoids in L. zeylanica and henceforth conferred protection against ethanol and H2O2-induced oxidative stress on hepatic tissue.DOI: http://dx.doi.org/10.3329/icpj.v2i9.16076 International Current Pharmaceutical Journal, August 2013, 2(9): 148-151

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Antioxidative Role of Hatikana (Leea macrophyllaRoxb.) Partially Improves the Hepatic Damage Induced by CCl4in Wistar Albino Rats

BioMed Research International ◽

10.1155/2015/356729 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 8

Author(s):

Samina Akhter ◽

Md. Atiar Rahman ◽

Jannatul Aklima ◽

Md. Rakibul Hasan ◽

J. M. Kamirul Hasan Chowdhury

Keyword(s):

Alkaline Phosphatase ◽

Liver Injury ◽

Total Protein ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Biochemical Parameters ◽

Lipid Profiles ◽

Scavenging Effect ◽

Serum Aspartate Aminotransferase

This research investigated the protective role ofLeea macrophyllaextract on CCl4-induced acute liver injury in rats. Different fractions ofLeea macrophylla(Roxb.) crude extract were subjected to analysis for antioxidative effects. Rats were randomly divided into four groups as normal control, hepatic control, and reference control (silymarin) group and treatment group. Evaluations were made for the effects of the fractions on serum enzymes and biochemical parameters of CCl4-induced albino rat. Histopathological screening was also performed to evaluate the changes of liver tissue before and after treatment. Different fractions ofLeea macrophyllashowed very potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect, FeCl3reducing effect, superoxide scavenging effect, and iron chelating effect. Carbon tetrachloride induction increased the level of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) and other biochemical parameters such as lipid profiles, total protein, and CK-MB. In contrast, treatment ofLeea macrophyllareduced the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) activities as well as biochemical parameters activities.L. macrophyllapartially restored the lipid profiles, total protein, and CK-MB. Histopathology showed the treated liver towards restoration. Results evidenced thatL. macrophyllacan be prospective source of hepatic management in liver injury.

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