Effect of resistive-endurance exercises on myocardial tissue creatine kinase isoenzyme, IL-6 and IL-10 serum changes in male cardiovascular patients

Objective: The current research was designed to evaluate the cardioprotective activity of Mentha longifolia (ML) leaf extract on cyclophosphamide-induced cardiotoxicity in rats.Methods: Cardiotoxicity was induced in Albino wistar rats of either sex by administering a single injection of cyclophosphamide (200 mg/kg, i. p.) on the first day of the experimental period. Mentha longifolia (250 and 500 mg/kg, p. o.) was administered daily for 10 d immediately after administration of cyclophosphamide on the first day. The general observations such as oxidative marker enzyme assays, ECG and histopathology were examined.Results: Cyclophosphamide administration significantly (p<0.05) increased lipid peroxidation (LPO) and decreased the levels of antioxidant markers such as superoxide dismutase (SOD) and catalase (CAT). Cyclophosphamide elevated the levels of biomarker enzymes like creatine kinase isoenzyme MB (CK-MB), creatine kinase isoenzyme NAC (CK-NAC) and lactate dehydrogenase (LDH). Further, the cyclophosphamide-treated rats showed changes in electrocardiographic parameters. Treatment with Mentha longifolia significantly (p<0.05) reversed the status of cardiac biomarkers, ECG and oxidative enzymes in cyclophosphamide-induced cardiotoxicity. Histopathological examination was also supported the potential cardioprotective effect of Mentha longifolia with reduced damage to the myocardium.Conclusion: The biochemical, ECG and histopathology reports support the potential benefits of Mentha longifolia against myocardial damage which could be attributed to antioxidant activity.

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Creatine Kinase Isoenzyme DB in Cerebrospinal Fluid from Patients with Meningitis and Encephalitis

The Journal of Infectious Diseases ◽

10.1093/infdis/148.1.180 ◽

1983 ◽

Vol 148 (1) ◽

pp. 180-180 ◽

Cited By ~ 8

Author(s):

H. Briem ◽

L. Lindquist ◽

P. Lundbergh ◽

E. Sego ◽

B. Skoldenberg

Keyword(s):

Cerebrospinal Fluid ◽

Creatine Kinase ◽

Creatine Kinase Isoenzyme

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Appearance of a cathodic band in the electrophoretogram of blood creatine kinase isoenzyme-MM fraction during hypoxia in rats.

Clinical Chemistry ◽

10.1093/clinchem/31.4.533 ◽

1985 ◽

Vol 31 (4) ◽

pp. 533-536 ◽

Cited By ~ 5

Author(s):

T Hayashi ◽

T Tanaka

Keyword(s):

Creatine Kinase ◽

Cardiac Muscle ◽

Mitochondrial Fraction ◽

Recovery Stage ◽

Creatine Kinase Isoenzyme ◽

Myocardial Mitochondria ◽

Hypoxic Conditions ◽

Cathodic Side ◽

Mitochondrial Origin

Abstract In electrophoretograms of creatine kinase (CK; EC 2.7.3.2) in patients' blood, a band, presumably of mitochondrial origin, is occasionally observed on the cathodic side of the CK-MM fraction. We studied the implications of this phenomenon in rats exposed to hypoxic conditions. In the hypoxic cardiac muscle, the proportions of CK-MB and CK-MM were not significantly different from controls, but that of the mitochondrial CK was lower. In the corresponding blood, the cathodic mitochondrial CK band appeared, but disappeared as the animals recovered from hypoxia. The CK-MB isoenzyme was increased in the blood of the control rats, as obtained by heart puncture, but no mitochondrial fraction was detected. We believe that changes in myocardial mitochondria during hypoxia are related to the appearance of the cathodic band. Cytoplasmic CK-MB, unlike mitochondrial CK, markedly increased in the rats' blood during the recovery stage rather than during the hypoxia.

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Assay of creatine kinase isoenzyme MB in serum with time-resolved immunofluorometry

Clinical Chemistry ◽

10.1093/clinchem/36.9.1679 ◽

1990 ◽

Vol 36 (9) ◽

pp. 1679-1683 ◽

Cited By ~ 2

Author(s):

E S Lianidou ◽

T K Christopoulos ◽

E P Diamandis

Keyword(s):

Creatine Kinase ◽

Laser Excitation ◽

Solid Phase ◽

Time Resolved ◽

Creatine Kinase Isoenzyme ◽

Phase Complex ◽

Precision And Accuracy ◽

Accuracy Indices ◽

First Time ◽

Creatine Kinase Isoenzyme Mb

Abstract We describe the first time-resolved immunofluorometric assay for creatine kinase (EC 2.7.3.2) isoenzyme MB (CK-MB) in serum. The assay is based on the formation of the complex: solid-phase anti-CK-MB-CK-MB-biotinylated anti-CK-BB-streptavidin-BCPDA-Eu3+, where anti-CK-MB and anti-CK-BB are monoclonal antibodies against the CK isoenzymes MB and BB, respectively, and BCPDA is the europium chelator 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid. The solid-phase complex is fluorescent and is measured on the dry solid-phase (microtiter well) in a specially designed time-resolved fluorometer that uses laser excitation. The assay requires 25 microL of serum and is not affected by the presence of either CK-MM (up to 5000 micrograms/L) or CK-BB (up to 1000 micrograms/L) in the sample. Precision and accuracy indices for the assay were satisfactory.

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Cardioprotective Effects of Glycyrrhiza uralensis Extract Against Doxorubicin- Induced Toxicity

International Journal of Toxicology ◽

10.1177/1091581810393033 ◽

2011 ◽

Vol 30 (2) ◽

pp. 181-189 ◽

Cited By ~ 10

Author(s):

Ling Zhang ◽

Yinxin Yang ◽

Lingyan Yu ◽

Yi Wang ◽

Li Liu ◽

...

Keyword(s):

Creatine Kinase ◽

Lactate Dehydrogenase ◽

Serum Levels ◽

Inhibitory Effect ◽

Heart Tissue ◽

Glycyrrhiza Uralensis ◽

Creatine Kinase Isoenzyme ◽

Imprinting Control Region ◽

Cardioprotective Effects ◽

Histopathologic Analysis

The purpose of the present study was to investigate the cardioprotective effects of Glycyrrhiza uralensis extract (GUE) against doxorubicin (DOX)-induced cardiotoxicity. Imprinting control region (ICR) mice were treated with saline, DOX (20 mg/kg intraperitoneal [ip] for once), GUE (100 mg/kg intragastric [ig] for 8 days), co-treatments with DOX and GUE (100 mg/kg ig for 8 days), and amifostine (100 mg/kg intravenous [iv] for once), respectively. Serum levels of lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB), glutathione peroxidase (GSH-PX) activity, and glutathione (GSH) level in heart tissue were measured. Histopathologic analysis of heart tissue was also performed. Treatment with GUE significantly protected the mice from DOX-induced cardiotoxicity, indicated by decreased levels of serum LDH and CK-MB, improved heart morphology and increased GSH-PX activity and GSH level. Additionally, GUE did not compromise the tumor-inhibitory effect of DOX. In conclusion, our studies imply the potentially clinical application of GUE to overcome the cardiotoxicity of doxorubicin.

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Creatine kinase isoenzyme BB in human amniotic fluid.

Clinical Chemistry ◽

10.1093/clinchem/26.12.1756a ◽

1980 ◽

Vol 26 (12) ◽

pp. 1756-1756 ◽

Cited By ~ 1

Author(s):

T J Delahunty ◽

C C Foreback

Keyword(s):

Creatine Kinase ◽

Amniotic Fluid ◽

Human Amniotic Fluid ◽

Creatine Kinase Isoenzyme

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Increased creatine kinase isoenzyme MB values in patients without myocardial infarct.

Clinical Chemistry ◽

10.1093/clinchem/24.10.1818 ◽

1978 ◽

Vol 24 (10) ◽

pp. 1818-1821 ◽

Cited By ~ 3

Author(s):

L M Shaw ◽

D A Newman

Keyword(s):

Myocardial Infarction ◽

Intensive Care Unit ◽

Creatine Kinase ◽

Medical Intensive Care Unit ◽

Myocardial Infarct ◽

Creatine Kinase Isoenzyme ◽

Creatine Kinase Mb ◽

Medical Intensive Care ◽

Column Procedure ◽

Creatine Kinase Isoenzyme Mb

Abstract Six of 13 randomly selected patients in a medical intensive-care unit with above-normal creatine kinase MB activities had diagnoses other than myocardial infarction. These data, which indicate the need for further study, were obtained during evaluation of a commercially available column procedure (Biodynamics/bmc).

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