scholarly journals FOREST SEED REGIONS OF SCOTS PINE BASED ON GROWTH ASSESSEMENT IN THE PROVENANCE TRIAL IN SIBERIA

Keyword(s):  
2015 ◽  
Vol 67 (4) ◽  
pp. 1237-1249
Author(s):  
Piotr Androsiuk ◽  
Sylwia Ciaglo-Androsiuk ◽  
Lech Urbaniak

DNA markers have become effective tools in genetic diversity studies of forest trees. However, molecular marker analyses are associated with laborious and costly effort. One of the possibilities to overcome these constraints is to analyze bulked samples per population, rather than individual plants. We have used bulked DNA-based AFLP analysis to investigate genetic variations in Pinus sylvestris L. (Scots pine) from the IUFRO 1982 provenance trial in K?rnik (western Poland). Four AFLP primer combinations yielded a total of 309 bands, of which 208 (67.31%) were polymorphic. Thirty-six (11.65%) unique alleles were deployed randomly among the populations. Estimated genetic diversity and differentiation was high, as expressed by He = 0.238 and I = 0.356, and by genetic distance values which ranged from 0.154 to 0.363. A geographic pattern of interpopulation differentiation was observed, pointing to the individual character of populations from northeastern Europe. In the light of available data, we discuss the influence of historical migration routes, gene flow and human activity on observed genetic diversity and differentiation of Scots pine in Europe. Our results indicate that the AFLP method applied to DNA templates extracted from bulked leaf samples provides an efficient approach to elucidate genetic diversity and relationships among Scots pine populations.


2014 ◽  
Vol 63 (3-4) ◽  
pp. 315-324 ◽  
Author(s):  
Jerzy Szweykowski ◽  
Wiesław Prus-Głowacki ◽  
Jerzy Hrynkiewicz

The comparison of the genetic stucture of the population of Scots pine from Szczeliniec Wielki Mt. with the population of its progeny and three other populations from the same geographic region shows great similarity of both populations as far as the frequencies of genes and genotypes are concerned. Also other genetic parameters, such as heterozygosity, genotypic polymorphism indices and a number of alleles per locus were in both populations very similar. It seems that our data proved that the provenance trial experiments reflect rather closely the genetic structure of maternal populations. The fact that the population from Szczeliniec Wielki and its "replica" are genetically most similar to the geographically closest population from Bystrzyca Kłodzka leads to the conclusion, that these populations are of common origin. Though the relic character of the population from Szczeliniec can not be excluded. The morphological distinctness of the population from Szczeliniec is caused by a modifying influence of environmental conditions.


2020 ◽  
Vol 10 (2) ◽  
pp. 60-69
Author(s):  
Mariya Mihaylova ◽  
Mikhail Chernyshov

The indicators characterizing growth, productivity and living conditions of forest-steppe and steppe ecotypes of Scots pine in provenance trial plantation of Stupinsky Pole training ground in the Voronezh Region, obtained in 2019 on temporary sample plots, have been analyzed. The object of comprehensive research are forest-steppe and steppe ecotypes of Scots pine in 60-year-old provenance trial plantations growing in the Ramonsky district forestry of the Voronezh forestry. The studied cultures were laid on the lands after agricultural use in 1959 under the guidance of Professor M. M. Veresin. The planting material was 2-year-old standard seedlings of Scots pine, planted manually under Kolesov's planting iron. The type of growing conditions is A2 and B2. No additional planting was made. Based on the materials of 13 test plots laid according to the standard method, some features of the growth of forest-steppe and steppe pine ecotypes has been revealed. Their productivity and living condition have been evaluated. The highest average height is a characteristic of the steppe ecotype from the seeds of the Valsky forestry of the Saratov region and the forest-steppe ecotype from the seeds of the Platonov forestry of the Tambov region. The lowest average height is in crops of the forest-steppe ecotype from the seeds of the Monastic forestry of the Penza region. Local ecotypes (seeds from the Khrenovsky forestry) have the largest average diameter. The steppe ecotypes from the Saratov Region have the smallest diameter. The variability of the diameters of the preserved 60-year-old trees at a height of 1.3 m and at the surface of the soil was estimated as average - 16.99-31.09% and 19.50-29.94%, respectively. It is also typical for ordinary forest plantations. All the trees are classified as industrial trees. The stock of raw wood depends on the stand density. The largest stock was recorded in cultures from the Bryansk region (Krasnoslobodskoye forestry) due to the greater number of preserved trees. The smallest is among forest-steppe ecotypes of the Belgorod region. The analysis of the vital state of the surviving plants has showed that the best indicators have forest-steppe ecotypes from adjacent areas with similar climatic conditions. At the same time, the amount of preserved pine trees of all ecotypes per 1 ha is not large. Their preservation is 3.8-10.2% of the trees planted in 1959. Preservation varies greatly in ecotypes. Edaphic conditions for the growth of maternal stands where seeds were harvested have a significant impact on the living conditions of pine provenance trial plantations


2014 ◽  
Vol 7 (1) ◽  
pp. 84-89 ◽  
Author(s):  
N. V. Pakharkova ◽  
N. A. Kuzmina ◽  
S. R. Kuzmin ◽  
A. A. Efremov
Keyword(s):  

2019 ◽  
Vol 88 (2) ◽  
Author(s):  
Anna Maria Hebda ◽  
Witold Wachowiak

We studied the genetic variation in a set of nuclear genes analyzed from 16 populations of Scots pine derived from a 50-year-old provenance trial in Poland. At the same set of loci, the pattern of genetic variation was compared to several reference populations from a latitudinal gradient in Northern and Central Europe. Similar levels of nucleotide diversity were observed between the defined groups of Polish populations representing three climatic regions (π<sub>total</sub> = 0.0040–0.0051) in comparison with the reference samples (π<sub>total</sub> = 0.0054–0.0058). Polish populations showed minor but heterogeneous patterns of genetic variation between regional groups (<em>F</em><sub>ST</sub> up to 6%), which were caused by differentiation at specific loci. When outlier loci were excluded from between group comparisons, there were no differences between the Polish populations. Loci related to glycosyltransferase and laccase were identified as outliers, and were correlated with phenotypic differentiation using mixed-linear models. Moreover, these genes were also found as being potentially under selection across the Scots pine distribution range as the patterns of nucleotide variation correlated with latitude and altitude of the maternal stands. The provenance trial measurements have characterized a set of growth and developmental traits over 50 years and forms a suitable experimental system for detailed genetic studies.


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