scholarly journals Effects of different extraction methods on the properties of pine cone polysaccharides from Pinus koraiensis

BioResources ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. 9945-9956
Author(s):  
Qiang Zhao ◽  
Jie Qin ◽  
Hairong Wang ◽  
Jingyu Wang ◽  
Xueming Zhang
Keyword(s):  
Monosaccharide Composition ◽  
Extraction Process ◽  
Extraction Methods ◽  
Hot Water ◽  
Water Extraction ◽  
Pinus Koraiensis ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Hot Water Extraction ◽  
Pine Cone

In order to realize the resource utilization of the Pinus koraiensis pine cone, the polysaccharides were obtained by a traditional hot water extraction process, and the optimal extraction process was confirmed. The effects of a traditional hot water, alkali, and ultrasound assisted extraction on the yield and properties of polysaccharides were studied. The structure, monosaccharide composition, and content of polysaccharides obtained by different extraction methods were analyzed by means of Fourier infrared chromatography and liquid chromatography. Effects of polysaccharides extracted by different methods on the antimicrobial activity of Staphylococcus aureus and Escherichia coli were studied. The scavenging effect of DPPH free radicals were also examined. The optimum condition of the hot water extraction was identified as an extraction temperature of 100 °C, a ratio of material to water at 1:16 (w/v), an extraction time of 4 h, and a yield of polysaccharide at 10.2%. In comparison, the yield of the pine cone polysaccharide was improved significantly to 29.1% by using NaOH solution extraction method. The FTIR spectra of polysaccharides showed that the samples possessed the typical chemical structure of a polysaccharide.

scholarly journals The Effect of Temperature on Pressurised Hot Water Extraction of Pharmacologically Important Metabolites as Analysed by UPLC-qTOF-MS and PCA

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
B. S. Khoza ◽  
L. Chimuka ◽  
E. Mukwevho ◽  
P. A. Steenkamp ◽  
N. E. Madala
Keyword(s):  
Antioxidant Activities ◽  
Pure Water ◽  
Principal Component ◽  
Extraction Process ◽  
Extraction Methods ◽  
Hot Water ◽  
Water Extraction ◽  
Effect Of Temperature ◽  
Extraction Temperature ◽  
Hot Water Extraction

Metabolite extraction methods have been shown to be a critical consideration for pharmacometabolomics studies and, as such, optimization and development of new extraction methods are crucial. In the current study, an organic solvent-free method, namely, pressurised hot water extraction (PHWE), was used to extract pharmacologically important metabolites from driedMoringa oleiferaleaves. Here, the temperature of the extraction solvent (pure water) was altered while keeping other factors constant using a homemade PHWE system. Samples extracted at different temperatures (50, 100, and 150°C) were assayed for antioxidant activities and the effect of the temperature on the extraction process was evaluated. The samples were further analysed by mass spectrometry to elucidate their metabolite compositions. Principal component analysis (PCA) evaluation of the UPLC-MS data showed distinctive differential metabolite patterns. Here, temperature changes during PHWE were shown to affect the levels of metabolites with known pharmacological activities, such as chlorogenic acids and flavonoids. Our overall findings suggest that, if not well optimised, the extraction temperature could compromise the “pharmacological potency” of the extracts. The use of MS in combination with PCA was furthermore shown to be an excellent approach to evaluate the quality and content of pharmacologically important extracts.

Effect of Ultrasonic Wave on Lentinan Extraction

2011 ◽  
Vol 291-294 ◽  
pp. 1339-1343
Author(s):  
Wen Bo Zhang ◽  
Hong Rui Li ◽  
Jun Tao ◽  
Bing Bing Dong
Keyword(s):  
Extraction Efficiency ◽  
Hot Water ◽  
Water Extraction ◽  
Extraction Technique ◽  
Orthogonal Experimental Design ◽  
Extraction Temperature ◽  
Lentinus Edodes ◽  
Extraction Condition ◽  
Hot Water Extraction ◽  
Extraction Parameters

The research in this paper optimized the extraction technique of lentinan with ultrasonic assistant method on the basis of hot water extraction technique, and investigated the promoting function of ultrasound to polysaccharides extraction. Extraction condition was selected by means of orthogonal experimental design, four factors and three levels L9(34), after key elements were respectively chosen through single factor experiments. Comparison between optimal extraction parameters of two method, hot water extraction technique and ultrasonic assistant extraction technique, showed decreased extraction temperature and significantly shortened extraction time, which existed in the second means, improved the extraction efficiency. Lentinus edodes polysaccharide extracted with ultrasonic assistant technique, the extraction rate and polysaccharide content percentage increased 6.22% and 8.66% respectively, comparative to which extracted with hot water extraction technique.

scholarly journals Effects of non-traditional extraction methods on extracting bioactive compounds from chaga mushroom (Inonotus obliquus) compared with hot water extraction

LWT ◽  
2019 ◽  
Vol 110 ◽  
pp. 80-84 ◽  
Author(s):  
Ah Young Hwang ◽  
Si Chang Yang ◽  
Jaecheol Kim ◽  
Taehwan Lim ◽  
Hyunnho Cho ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Extraction Methods ◽  
Hot Water ◽  
Water Extraction ◽  
Inonotus Obliquus ◽  
Hot Water Extraction

scholarly journals Comparing of hot water and acid extraction of polysaccharides from proso millet

2017 ◽  
Vol 23 (1) ◽  
pp. 141-150
Author(s):  
Aishi Zhu ◽  
Shanshan Liu ◽  
Kanfeng Wu ◽  
Chuan Ren ◽  
Maoqian Xu
Keyword(s):  
Polynomial Equation ◽  
Hot Water ◽  
Water Extraction ◽  
Acid Extraction ◽  
Extraction Temperature ◽  
Hot Water Extraction ◽  
Solid Ratio ◽  
Proso Millet ◽  
Acid Aqueous Solution ◽  
Experimental Yield

The extraction of polysaccharides from proso millet was investigated experimentally using hot water and acid aqueous solution. Response surface methodology, based on a three-level, three- or four-variable Box-Behnken design for hot water extraction or acid extraction, respectively, was employed to obtain the best possible combination of acid concentration, liquid-solid ratio, extraction time, and extraction temperature for maximum polysaccharides yield. The obtained experimental data were fitted to a second-order polynomial equation and analyzed by appropriate statistical methods. The corresponding optimum extraction conditions of each method were obtained. Under the optimum conditions, the experimental yield was well in close agreement with the predicted value by the model. The results showed that the polysaccharides yield of acid extraction was 42.13 mg g-1, significantly higher than 20.07 mg g-1 of the yield of hot water extraction, the obtained equation could be used to predict the extraction experimental results.

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