scholarly journals [Management, Facility] [Anesthesia] [Metabolism, Ageing] [Neurology, Behaviour] [Pathology, Morphometry] [Reproduction, ES cells] [Gene Function and Analysis] [Microbiology, Infectious Diseases] [Animal Models] [Bioresource]

2013 ◽  
Vol 62 (Supplement) ◽  
pp. S84-S133
Keyword(s):  
Animal Models ◽  
Infectious Diseases ◽  
Gene Function ◽  
Es Cells

scholarly journals INTRODUCTION: Immune Relevant Animal Models: Opportunities and Challenges

ILAR Journal ◽  
2018 ◽  
Vol 59 (3) ◽  
pp. 209-210
Author(s):  
Gregers Jungersen ◽  
Jorge Piedrahita
Keyword(s):  
Immune System ◽  
T Cell ◽  
Animal Models ◽  
Infectious Diseases ◽  
Tissue Specific ◽  
Car T Cell ◽  
Car T ◽  
Preclinical Animal Models

Abstract Valid interpretation of preclinical animal models in immunology-related clinical challenges is important to solve outstanding clinical needs. Given the overall complexity of the immune system and both species- and tissue-specific immune peculiarities, the selection and design of appropriate immune-relevant animal models is, however, not following a straightforward path. The topics in this issue of the ILAR Journal provide assessments of immune-relevant animal models used in oncology, hematopoietic-, CAR-T cell- and xenotransplantation, adjuvants and infectious diseases, and immune privileged inflammation that are providing key insights into unmet human clinical needs.

scholarly journals Contemporary approaches for modifying the mouse genome

2008 ◽  
Vol 34 (3) ◽  
pp. 225-238 ◽  
Author(s):  
David J. Adams ◽  
Louise van der Weyden
Keyword(s):  
Gene Function ◽  
New Technologies ◽  
Mouse Genome ◽  
Es Cells ◽  
Embryonic Stem ◽  
Reverse Genetic ◽  
Es Cell ◽  
Genetic Screens ◽  
Experimental Organism

The mouse is a premiere experimental organism that has contributed significantly to our understanding of vertebrate biology. Manipulation of the mouse genome via embryonic stem (ES) cell technology makes it possible to engineer an almost limitless repertoire of mutations to model human disease and assess gene function. In this review we outline recent advances in mouse experimental genetics and provide a “how-to” guide for those people wishing to access this technology. We also discuss new technologies, such as transposon-mediated mutagenesis, and resources of targeting vectors and ES cells, which are likely to dramatically accelerate the pace with which we can assess gene function in vivo, and the progress of forward and reverse genetic screens in mice.

scholarly journals A Rapid and Highly Efficient Genotyping Pipeline for Screening Gene Targeted Mouse Embryonic Stem Cells

2021 ◽  
Author(s):  
Roger Caothien ◽  
Charles Yu ◽  
Lucinda Tam ◽  
Robert Newman ◽  
Brian Nakao ◽  
...  
Keyword(s):  
Animal Models ◽  
Gene Targeting ◽  
Fluorescent Protein ◽  
Es Cells ◽  
Expression Patterns ◽  
Embryonic Stem ◽  
Rapid Screening ◽  
Es Cell ◽  
Screening Process ◽  
Targeting Vector

Abstract Gene targeting in mouse ES cells replaces or modifies genes of interest; conditional alleles, reporter knock-ins, and amino acid changes are common examples of how gene targeting is used. For example, enhanced green fluorescent protein or Cre recombinase is placed under the control of endogenous genes to define promoter expression patterns. The most important step in the process is to demonstrate that a gene targeting vector is correctly integrated in the genome at the desired chromosomal location. The rapid identification of correctly targeted ES cell clones is facilitated by proper targeting vector construction, rapid screening procedures, and advances in cell culture. The addition of magnetic activated cell sorting (MACS) technology and multiplex droplet digital PCR (ddPCR) to the ES cell screening process can achieve a greater than 60% assurance that ES clones are correctly targeted. In a further refinement of the process, drug selection cassettes are removed from ES cells with adenovirus technology. This improved workflow reduces the time needed to generate preclinical animal models. Faster access to animal models for therapeutic target identification and experimental validation can accelerate the development of therapies for human disease.

scholarly journals Imaging of Small-Animal Models of Infectious Diseases

2013 ◽  
Vol 182 (2) ◽  
pp. 296-304 ◽  
Author(s):  
Linda A. Jelicks ◽  
Michael P. Lisanti ◽  
Fabiana S. Machado ◽  
Louis M. Weiss ◽  
Herbert B. Tanowitz ◽  
...  
Keyword(s):  
Animal Models ◽  
Infectious Diseases ◽  
Small Animal ◽  
Small Animal Models

scholarly journals The science and medicine of human immunology

Science ◽  
2020 ◽  
Vol 369 (6511) ◽  
pp. eaay4014
Author(s):  
Bali Pulendran ◽  
Mark M. Davis
Keyword(s):  
Immune System ◽  
Animal Models ◽  
Infectious Diseases ◽  
Autoimmune Diseases ◽  
Inbred Mice ◽  
Human Immune System ◽  
Human Immunology ◽  
Human Immune ◽  
Mechanistic Understanding ◽  
New Strategies

Although the development of effective vaccines has saved countless lives from infectious diseases, the basic workings of the human immune system are complex and have required the development of animal models, such as inbred mice, to define mechanisms of immunity. More recently, new strategies and technologies have been developed to directly explore the human immune system with unprecedented precision. We discuss how these approaches are advancing our mechanistic understanding of human immunology and are facilitating the development of vaccines and therapeutics for infection, autoimmune diseases, and cancer.

scholarly journals Recombinant Varicella-Zoster Virus Vaccines as Platforms for Expression of Foreign Antigens

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Wayne L. Gray
Keyword(s):  
Older Adults ◽  
Animal Models ◽  
Infectious Diseases ◽  
Varicella Zoster Virus ◽  
The Elderly ◽  
Recombinant Vaccines ◽  
Varicella Zoster

Varicella-zoster virus (VZV) vaccines induce immunity against childhood chickenpox and against shingles in older adults. The safety, efficacy, and widespread use of VZV vaccines suggest that they may also be effective as recombinant vaccines against other infectious diseases that affect the young and the elderly. The generation of recombinant VZV vaccines and their evaluation in animal models are reviewed. The potential advantages and limitations of recombinant VZV vaccines are addressed.

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