Optimization of media for in vitro plant cultivation by the example of water sorrel (Rumex aquaticus L.)

Author(s):  
M.V. Skaptsov ◽  
◽  
D.V. Balabova ◽  
M.G. Kutsev ◽  
◽  
...  
2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Fernanda Engel ◽  
Antônio Azeredo Coutinho Neto

One of the major problems of in vitro plant cultivation is caused by contaminations such asfungal, which represent great losses for plant cultivation laboratories. In view of the problem about contamination and gaps in efficient disinfection protocols, the present study aimed to evaluate the inhibition of the fungus Colletotrichum. lindemuthianum in the presence of the potassium sorbate food preservative at different concentrations (0 g L-1; 0.076 g. L-1; 0.15 g. L-1; 0.30 g. L-1; 0.45 g. L-1; 0.60 g. L-1 and 0.75 g. L-1). The analyses of the Percentage of Micelal Growth Inhibition (ICP) and the growth at 24 and 72 hours at different concentrations were quantified. It was observed that Potassium Sorbate was effective in the analysis of ICP at different concentrations in relation to control, with greater effectiveness for T4 to T7. The highest percentages of inhibition (T5 to T7) presenting an inhibition rate of mean mycelial growth of 17.5 % in relation to the control for the 72h period, indicating, therefore, that with further studies this preservative can be used to combat fungal microorganisms in vitro culture.


2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  

1999 ◽  
Vol 155 (3) ◽  
pp. 376-386 ◽  
Author(s):  
Linda K. Adams ◽  
Erica E. Benson ◽  
Harry J. Staines ◽  
David H. Bremner ◽  
Stephen Millam ◽  
...  

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