ГЕНЕТИЧЕСКАЯ СТРУКТУРА ЕСТЕСТВЕННЫХ ПОПУЛЯЦИЙ СТЕРЛЯДИ (Acipenser ruthenus L.) В БАССЕЙНАХ РЕК КАМА И ОБЬ НА ОСНОВАНИИ ПОЛИМОРФИЗМА ISSR МАРКЕРОВ

2018 ◽  
Vol 53 (2) ◽  
pp. 348-354 ◽  
Author(s):  
Л.В. КОМАРОВА ◽  
◽  
Н.В. КОСТИЦЫНА ◽  
С.В. БОРОННИКОВА ◽  
А.Г. МЕЛЬНИКОВА ◽  
...  
Keyword(s):  
Acipenser Ruthenus

USING CRYOPRESERVED SPERM FOR CREATING STERLET BROOD STOCK

2017 ◽  
pp. 118-127
Author(s):  
Elena Nikolaevna Ponomareva ◽  
Maria Mikhailovna Belaya ◽  
Alexandra Andrianovna Krasilnikova ◽  
Alexander Nickolaevich Nevalennyy
Keyword(s):  
Liquid Nitrogen ◽  
Control Group ◽  
Test Results ◽  
Brood Stock ◽  
Active Response ◽  
Acipenser Ruthenus ◽  
Rostov Region ◽  
The Central Nervous System ◽  
Cryopreserved Sperm ◽  
Sturgeon Fish

The research on the sterlet roe artificial insemination using cryopreserved sperm was carried out in the research base of the RAS Southern Scientific Centre (the Rostov region). Reproductive cells (including cryopreserved cells), larvae, sterlet fry ( Acipenser ruthenus Linnaeus, 1758) were taken as an object of research. A half of the roe (1.7 kg) taken from female starlet was inseminated by native sperm (control group); another half was inseminated by defrosted sperm of two males, which was stored in liquid nitrogen at -196ºC during 3 years (pilot group). Incubation lasted 5 days at water temperature 14.5-18.2ºC, with daily fluctuations of temperature 1.9ºC. Roe insemination in the control group made 90%, in the pilot group - 70%. Roe embryonic growth in the control group was faster, but embryogenesis duration in the pilot group met the standard time limits. Hatching prolarvae in the control group started one hour earlier, than in the pilot group; it made 75% and 60% of all incubated roe, correspondingly. Waste during the period of larvae maturing before they pass to mixed feeding was negligible - 2% in the control group and 3.4% in the pilot group. According to the test results, "open field" of reactivity of the central nervous system in the pilot group fry didn’t change from the control group fry, but more active response to stimuli was noted in the pilot group, which is very important for fry adaptation to the conditions in natural water basins. It was established that sterlet offspring obtained with use of defrosted sexual cells does not differ from the offspring obtained using native sperm and has higher morphometric characteristics. The test results prove the possibility and practicability of using sexual cells stored in liquid nitrogen for artificial restoration and formation of sturgeon fish broodstocks.

scholarly journals Ancient Sturgeons Possess Effective DNA Repair Mechanisms: Influence of Model Genotoxicants on Embryo Development of Sterlet, Acipenser ruthenus

2020 ◽  
Vol 22 (1) ◽  
pp. 6
Author(s):  
Ievgeniia Gazo ◽  
Roman Franěk ◽  
Radek Šindelka ◽  
Ievgen Lebeda ◽  
Sahana Shivaramu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Damage ◽  
Dna Repair ◽  
Embryo Development ◽  
Hatching Rate ◽  
Embryo Survival ◽  
Dna Repair Mechanisms ◽  
Acipenser Ruthenus ◽  
Repair Mechanisms ◽  
Sterlet Acipenser Ruthenus

DNA damage caused by exogenous or endogenous factors is a common challenge for developing fish embryos. DNA damage repair (DDR) pathways help organisms minimize adverse effects of DNA alterations. In terms of DNA repair mechanisms, sturgeons represent a particularly interesting model due to their exceptional genome plasticity. Sterlet (Acipenser ruthenus) is a relatively small species of sturgeon. The goal of this study was to assess the sensitivity of sterlet embryos to model genotoxicants (camptothecin, etoposide, and benzo[a]pyrene), and to assess DDR responses. We assessed the effects of genotoxicants on embryo survival, hatching rate, DNA fragmentation, gene expression, and phosphorylation of H2AX and ATM kinase. Exposure of sterlet embryos to 1 µM benzo[a]pyrene induced low levels of DNA damage accompanied by ATM phosphorylation and xpc gene expression. Conversely, 20 µM etoposide exposure induced DNA damage without activation of known DDR pathways. Effects of 10 nM camptothecin on embryo development were stage-specific, with early stages, before gastrulation, being most sensitive. Overall, this study provides foundational information for future investigation of sterlet DDR pathways.

Fertility of females of sturgeon hybrids obtained from species with different levels of ploidy ( Acipenser ruthenus and A. dauricus ) and their cloning

2021 ◽  
Author(s):  
Victor P. Vasil'ev ◽  
Evgeniy I. Rachek ◽  
Dmitriy Yu. Amvrosov ◽  
Anna E. Barmintseva ◽  
Ekaterina D. Vasil'eva
Keyword(s):  
Acipenser Ruthenus ◽  
Different Levels

Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson
Keyword(s):  
Sperm Motility ◽  
Seminal Fluid ◽  
Mature Male ◽  
Sperm Maturation ◽  
Wolffian Duct ◽  
Testicular Spermatozoa ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus ◽  
Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

Ultrastructural Localization of Intracellular Calcium During Spermatogenesis of Sterlet (Acipenser ruthenus)

2016 ◽  
Vol 22 (6) ◽  
pp. 1155-1161 ◽  
Author(s):  
Amin Golpour ◽  
Martin Pšenička ◽  
Hamid Niksirat
Keyword(s):  
Intracellular Calcium ◽  
Developmental Stages ◽  
Physiological Function ◽  
Male Gamete ◽  
Ultrastructural Localization ◽  
Acrosomal Vesicle ◽  
Acipenser Ruthenus ◽  
Calcium Deposits ◽  
Late Stages ◽  
Sterlet Acipenser Ruthenus

AbstractCalcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. The aim of this study was to localize and quantify the intracellular distribution of calcium during different developmental stages of spermatogenesis in sterlet, Acipenser ruthenus, using a combined oxalate–pyroantimonate technique. The distribution of calcium was described in spermatogonium, spermatocyte, spermatid, and spermatozoon stages. In the spermatogonium and spermatocyte, calcium deposits were mainly localized in the nucleus and cytoplasm. The spermatid had calcium in the nucleus, developing acrosomal vesicle, and cytoplasm. Intracellular calcium transformed from scattered deposits in spermatogonia and spermatocyte stages into an unbound form in spermatid and the spermatozoon. The proportion of area covered by calcium increased significantly (p<0.05) from early to late stages of spermatogenesis. The largest proportion of area covered by calcium was observed in the nucleus of the spermatozoon. In conclusion, although most of the intracellular calcium is deposited in limited areas of the spermatogonium and spermatocyte, it is present an unbound form in the larger area of spermatids and spermatozoa which probably reflects changes in its physiological function and homeostasis during the process of male gamete production in spermatogenesis.

The role of the IGF-I system for vitellogenesis in maturing female sterlet, Acipenser ruthenus Linnaeus, 1758

2007 ◽  
Vol 150 (1) ◽  
pp. 140-150 ◽  
Author(s):  
S. Wuertz ◽  
A. Nitsche ◽  
M. Jastroch ◽  
J. Gessner ◽  
M. Klingenspor ◽  
...  
Keyword(s):  
Acipenser Ruthenus ◽  
Igf I ◽  
Sterlet Acipenser Ruthenus

COMMERCIAL FAUNA OF WA-TERBODIES IN THE MOSCOW RIVER BASIN IN THE SECOND HALF OF THE 18th CENTURY

2021 ◽  
pp. 94-112
Author(s):  
N. Ozerova
Keyword(s):  
River Basin ◽  
Fish Species ◽  
18Th Century ◽  
Commercial Fish ◽  
Acipenser Ruthenus ◽  
Land Survey ◽  
Ancient Times ◽  
In The Beginning ◽  
Almost All ◽  
Moscow River

Based on the data from economic notes to the General Land Survey, the ranges of commercial fish and crayfish species that inhabited waterbodies of the Moscow River basin in the second half of the 18th century are reconstructed. Eighteen maps showing the distribution of 22 fish species, including Acipenser ruthenus L., Abramis brama L., Barbatula barbatula L., Lota lota L., Sander lucioperca L. and others are compiled. Comparison of commercial fish species that lived in the Moscow River basin in the second half of the 18th century with data from ichthyological studies in the beginning of the XXI century and materials of archaeological surveys shows that almost all of these species have lived in the Moscow River basin since ancient times and have survived to the present day.

Sign in / Sign up

Export Citation Format

Share Document