scholarly journals REALIZATION OF NITROGEN FIXATION POTENTIAL OF TN5-MUTANTS Bradyrhizobium japonicum IN SYMBIOSIS WITH SOYBEAN PLANTS

2013 ◽  
Vol 6 (5) ◽  
pp. 122-130
Author(s):  
N. A. Vorobey ◽  
Keyword(s):  
Nitrogen Fixation ◽  
Bradyrhizobium Japonicum ◽  
Tn5 Mutants ◽  
Soybean Plants

scholarly journals Nodulation and Development of Soybean Submitted to Inoculation With Bradyrhizobium japonicum and Phosphorus Doses

2018 ◽  
Vol 10 (12) ◽  
pp. 321 ◽  
Author(s):  
Erica Chaves ◽  
Rubson da Costa Leite ◽  
Thalita Rodrigues Silva ◽  
Thayny Alves Viana ◽  
Tatiane de Sousa Cruz ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Experimental Design ◽  
Bradyrhizobium Japonicum ◽  
Phosphate Fertilizer ◽  
Dry Mass ◽  
Root Number ◽  
Phosphate Fertilization ◽  
Soybean Plants ◽  
Biological Nitrogen ◽  
Plant Dry Mass

Among the several factors that may influence nodulation and the efficiency of biological nitrogen fixation for soybean plants, nutrient availability is among the most important. This study aimed to evaluate the inoculation with Bradyrhizobium japonicum and doses of phosphorus on the development of soybean in a Vertisol, in Tocantins. The experimental design was completely randomized in a 4 × 2 factorial scheme, with four replications. Four doses of phosphate fertilization (0, 100, 200, and 300 kg ha-1 P2O5) were studied, combined with two inoculation treatments with Bradyrhizobium japonicum (inoculated and not inoculated). The following variables were evaluated: plant height, stem diameter, nodules per plant, dry mass of nodules, dry mass of plant, dry mass of root, number of pods and number of grains per pod. Under greenhouse conditions and soil with good availability of phosphorus, there is no influence of the doses on the inoculation with Bradyrhizobium japonicum. Soils with good availability of phosphorus have low response to the application of phosphate fertilizer.

scholarly journals THE EFFICIENCy OF DIFFERENT MEANS OF SOyBEAN INOCULATION wITH NODULE BACTERIA

2010 ◽  
Vol 10 ◽  
pp. 20-28
Author(s):  
S.M. Malichenko ◽  
V.K. Datsenko ◽  
P.M. Mamenko ◽  
S.Ya. Kots
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nitrogenase Activity ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Standard Strain ◽  
Bacterial Cultures ◽  
Tn5 Mutants ◽  
Soybean Plants ◽  
Production Strain

The efficiency of the presowing soybean inoculation with nodule bacteria at direct seeds bacterization or introduction of inoculum to the soil at presowing cultivation as well as the ability of rhizobia remained in the soil to the next year to form active symbioses with soybean plants was studied. The liquid bacterial cultures of production strain Bradyrhizobium japonicum 634b and three perspective Tn5-mutants of B. japonicum 646 were used. The introduction of the inoculum to soil was shown to be more efficient during both years of investigations as compared with the seeds inoculation which resulted in higher number of nodules formed, their nitrogenase activity and greater soybean seeds yield. Two of three studied Tn5-mutants had surpassed the standard strain by the efficiency indices.

scholarly journals PERSPECTIVE USE OF TN5-MUTANTS OF RHIZOBIA IN PREPARATION OF BACTERIAL FERTILIZERS

2009 ◽  
Vol 8 ◽  
pp. 32-39
Author(s):  
S.Ya. Kots ◽  
S.M. Malychenko ◽  
P.M. Mamenko ◽  
G.M. Drozdenko
Keyword(s):  
Escherichia Coli ◽  
Nitrogen Fixation ◽  
Bradyrhizobium Japonicum ◽  
Protein Composition ◽  
Transposon Mutagenesis ◽  
Nitrogen Fixation Activity ◽  
Fixation Activity ◽  
Tn5 Mutants

By using strain of Escherichia coli with build-in plasmid pSUP2021::Tn5 the transposon mutagenesis of Bradyrhizobium japonicum (strains 646, 614a and 71t) was performed. The highest formation frequency of kanamycinresistant mutants was 10-6 (B. japonicum 646). Among the 1500 mutants obtained, the best were selected by the “virulence”, “nitrogen fixation activity” and “symbiosis efficiency” criteria. In spite of different symbiotic characteristics of the selected Tn-5 mutants no considerable differences in their protein composition were determined. The authors made conclusion on the possibility of use of transposon mutagenesis in order to obtain the new forms of slowgrowing bacteria.

scholarly journals COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION

2016 ◽  
Vol 24 ◽  
pp. 29-36
Author(s):  
D. V. Krutylo
Keyword(s):  
Nitrogen Fixation ◽  
Field Experiment ◽  
Growth Rates ◽  
Bradyrhizobium Japonicum ◽  
Molecular Nitrogen ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Symbiotic System ◽  
Combined Use ◽  
Soybean Plants

In the vegetation experiment conditions it was established that the greatest effect of soybean bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inoculum in the ratio 1 : 1 (binary composition). In compared to the mono-inoculation the combined use of these strains contributed the formation of balanced symbiotic system, increase the level of molecular nitrogen fixation, content of chlorophyll in the biomass leaves and above ground mass of different soybean plants varieties on 7.8–19.6 %. In a field experiment inoculation of soybean seeds with a composition of B. japonicum strains to increase the soybean yields by 11.1–13.7 % compared to the mono-inoculation.

Involvement of Bradyrhizobium japonicum denitrification in symbiotic nitrogen fixation by soybean plants subjected to flooding

2011 ◽  
Vol 43 (1) ◽  
pp. 212-217 ◽  
Author(s):  
Cristina Sánchez ◽  
Germán Tortosa ◽  
Arsenio Granados ◽  
Antonio Delgado ◽  
Eulogio J. Bedmar ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Bradyrhizobium Japonicum ◽  
Symbiotic Nitrogen Fixation ◽  
Soybean Plants

scholarly journals EFFECT OF GOAT’S-RUE RHIZOBIA ON THE FORMATION AND FUNCTIONING OF THE SOYBEAN – BRADYRHIZOBIUM JAPONICUM 634B SYMBIOSIS

2019 ◽  
Vol 29 ◽  
pp. 29-36
Author(s):  
N. M. Melnykova ◽  
S. Ya. Kots
Keyword(s):  
Nitrogen Fixation ◽  
Root System ◽  
Bradyrhizobium Japonicum ◽  
Aerial Part ◽  
Nodule Formation ◽  
Nodule Bacteria ◽  
Rhizobial Symbiosis ◽  
Soybean Nodule ◽  
Soybean Plants ◽  
Observation Period

Objective. Study the peculiarities of nodule formation upon the formation of the symbiotic sys-tem soybean-Bradyrhizobium japonicum 634b, as well as the symbiotic nitrogen-fixation ability and plant growth and development under the influence of goat’s-rue rhizobia. Methods. Microbiologi-cal, physiological, statistical, gas chromatography. Results. In green house experiments, using sand as a substrate for growing plants, the mixed microbial cultures combining soybean nodule bacteria B. japonicum 634b and goat’s-rue nodule bacteria R. galegae 0702 or R. galegae 0703 in the ratio of 1 : 1 differed from the monoculture bradyrhizobium by their influence on the nodulation, nitro-gen-fixation ability of soybean-rhizobial symbiosis and development of soybean plants (variety Almaz). Increased nodulation activity in the primordial leaf and budding phases, as well as a signif-icant decrease in the level of symbiosis nitrogen fixation during budding, were observed when used in binary bacterial compositions of strain R. galegae 0703. These rhizobia of goat’s-rue suppressed the development of the root system of soybeans, but had no significant effect on the formation of the aerial part of the plants throughout the observation period. R. galegae 0702 strain slightly slowed the formation of nodules by bacteria in the primordial leaf phase, which caused a decrease in the number of soybean plants that formed symbiosis with B. japonicum 634b. Goat’s-rue nodule bacte-ria R. galegae 0702 improved the formation of the root system, and stimulated the growth and de-velopment of the aerial part of the macro symbiont in the phase of two trigeminal leaves. Conclu-sion. Combined inoculation of the rhizobia of goat’s-rue with nodule bacteria B. japonicum 634b showed a multidirectional effect on the formation of symbiosis by soybean plants of variety Almaz and functioning of soybean rhizobial symbiosis. The nature of the influence of R. galegae depended on their strain affiliation.

scholarly journals Effectiveness of symbiosis of soybean plants with Tn5-mutants of Bradyrhizobium japonicum 646

2014 ◽  
Vol 8 (2) ◽  
pp. 133-140
Author(s):  
V. P. Zаbоlоtnа ◽  
◽  
S. Ya. Коts ◽  
S. М. Mаlіchеnkо ◽  
◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Tn5 Mutants ◽  
Soybean Plants

Bradyrhizobium japonicum mutants defective in root-nodule bacteroid development and nitrogen fixation

1986 ◽  
Vol 144 (4) ◽  
pp. 355-366 ◽  
Author(s):  
B. Regensburger ◽  
L. Meyer ◽  
M. Filser ◽  
J. Weber ◽  
D. Studer ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Bradyrhizobium Japonicum ◽  
Root Nodule

scholarly journals Co-inoculation with Azospirillum brasilense in soybean cultivars subjected to water déficit

2020 ◽  
Vol 24 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Alessandra M. de L. Naoe ◽  
Joênes M. Peluzio ◽  
Leonardo J. M. Campos ◽  
Lucas K. Naoe ◽  
Roberta A. e Silva
Keyword(s):  
Grain Yield ◽  
Water Deficit ◽  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Field Experiments ◽  
Crop Evapotranspiration ◽  
Plant Genotype ◽  
Soybean Cultivars ◽  
Sowing Dates ◽  
Soybean Plants

ABSTRACT This study aimed to verify the effect of co-inoculation, association between Azospirillum brasilense and Bradyrhizobium japonicum bacteria, on soybean plants subjected to water deficit at two sowing dates. Two field experiments were conducted at the Universidade Federal de Tocantins, campus of Palmas, Brazil, in 2016. The experimental design was randomized blocks in a split-split-plot arrangement with four repetitions, where the plots consisted of two irrigation depths (100 and 25% of crop evapotranspiration - ETc), the subplots was composed of two methods of inoculant application (inoculation with Bradyrhizobium japonicum and co-inoculation with Azospirillum brasilense + Bradyrhizobium japonicum) and the sub-subplots comprised two soybean cultivars (TMG 132 and ANTA 82). The cultivars responded differently to the sowing dates. Co-inoculation did not influence grain yield under full irrigation conditions (100% ETc), in neither cultivar evaluated. However, under the water deficit condition (25% ETc), the grain yield of the cultivar TMG 132 increased 77.20%, indicating that there are different responses of interaction between Azospirillum brasilense, plant genotype and sowing dates.

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