scholarly journals EFFECT OF GOAT’S-RUE RHIZOBIA ON THE FORMATION AND FUNCTIONING OF THE SOYBEAN – BRADYRHIZOBIUM JAPONICUM 634B SYMBIOSIS

2019 ◽  
Vol 29 ◽  
pp. 29-36
Author(s):  
N. M. Melnykova ◽  
S. Ya. Kots
Keyword(s):  
Nitrogen Fixation ◽  
Root System ◽  
Bradyrhizobium Japonicum ◽  
Aerial Part ◽  
Nodule Formation ◽  
Nodule Bacteria ◽  
Rhizobial Symbiosis ◽  
Soybean Nodule ◽  
Soybean Plants ◽  
Observation Period

Objective. Study the peculiarities of nodule formation upon the formation of the symbiotic sys-tem soybean-Bradyrhizobium japonicum 634b, as well as the symbiotic nitrogen-fixation ability and plant growth and development under the influence of goat’s-rue rhizobia. Methods. Microbiologi-cal, physiological, statistical, gas chromatography. Results. In green house experiments, using sand as a substrate for growing plants, the mixed microbial cultures combining soybean nodule bacteria B. japonicum 634b and goat’s-rue nodule bacteria R. galegae 0702 or R. galegae 0703 in the ratio of 1 : 1 differed from the monoculture bradyrhizobium by their influence on the nodulation, nitro-gen-fixation ability of soybean-rhizobial symbiosis and development of soybean plants (variety Almaz). Increased nodulation activity in the primordial leaf and budding phases, as well as a signif-icant decrease in the level of symbiosis nitrogen fixation during budding, were observed when used in binary bacterial compositions of strain R. galegae 0703. These rhizobia of goat’s-rue suppressed the development of the root system of soybeans, but had no significant effect on the formation of the aerial part of the plants throughout the observation period. R. galegae 0702 strain slightly slowed the formation of nodules by bacteria in the primordial leaf phase, which caused a decrease in the number of soybean plants that formed symbiosis with B. japonicum 634b. Goat’s-rue nodule bacte-ria R. galegae 0702 improved the formation of the root system, and stimulated the growth and de-velopment of the aerial part of the macro symbiont in the phase of two trigeminal leaves. Conclu-sion. Combined inoculation of the rhizobia of goat’s-rue with nodule bacteria B. japonicum 634b showed a multidirectional effect on the formation of symbiosis by soybean plants of variety Almaz and functioning of soybean rhizobial symbiosis. The nature of the influence of R. galegae depended on their strain affiliation.

scholarly journals THE EFFECT OF STORAGE METHODS ON VIABILITY AND SYMBIOTIC PROPERTIES OF SOYBEAN NODULE BACTERIA

2014 ◽  
Vol 20 ◽  
pp. 15-22
Author(s):  
T. M. Kovalevska ◽  
D. V. Krutylo ◽  
V. P. Horban ◽  
I. M. Romanova ◽  
T. O. Usmanova
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nodule Bacteria ◽  
Partial Restoration ◽  
Long Term Storage ◽  
Soybean Nodule ◽  
Storage Methods ◽  
Soybean Plants ◽  
Symbiotic Properties ◽  
Term Storage

The viability and symbiotic properties of active strain Bradyrhizobium japonicum 46 after its long-term storage using different methods were studied. It was established that strain storing in evaporative freezing conditions as well at periodic passages the strain preserves its ability to form active symbiosis with soybean plants. Long-term storage under the layer of mineral oil had negatively influenced on the symbiotic properties of B. japonicum 46 strain resulted in only partial restoration of strains virulence, nitrogen fixing activity and competitiveness.

scholarly journals COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION

2016 ◽  
Vol 24 ◽  
pp. 29-36
Author(s):  
D. V. Krutylo
Keyword(s):  
Nitrogen Fixation ◽  
Field Experiment ◽  
Growth Rates ◽  
Bradyrhizobium Japonicum ◽  
Molecular Nitrogen ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Symbiotic System ◽  
Combined Use ◽  
Soybean Plants

In the vegetation experiment conditions it was established that the greatest effect of soybean bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inoculum in the ratio 1 : 1 (binary composition). In compared to the mono-inoculation the combined use of these strains contributed the formation of balanced symbiotic system, increase the level of molecular nitrogen fixation, content of chlorophyll in the biomass leaves and above ground mass of different soybean plants varieties on 7.8–19.6 %. In a field experiment inoculation of soybean seeds with a composition of B. japonicum strains to increase the soybean yields by 11.1–13.7 % compared to the mono-inoculation.

scholarly journals Nodulation and Development of Soybean Submitted to Inoculation With Bradyrhizobium japonicum and Phosphorus Doses

2018 ◽  
Vol 10 (12) ◽  
pp. 321 ◽  
Author(s):  
Erica Chaves ◽  
Rubson da Costa Leite ◽  
Thalita Rodrigues Silva ◽  
Thayny Alves Viana ◽  
Tatiane de Sousa Cruz ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Experimental Design ◽  
Bradyrhizobium Japonicum ◽  
Phosphate Fertilizer ◽  
Dry Mass ◽  
Root Number ◽  
Phosphate Fertilization ◽  
Soybean Plants ◽  
Biological Nitrogen ◽  
Plant Dry Mass

Among the several factors that may influence nodulation and the efficiency of biological nitrogen fixation for soybean plants, nutrient availability is among the most important. This study aimed to evaluate the inoculation with Bradyrhizobium japonicum and doses of phosphorus on the development of soybean in a Vertisol, in Tocantins. The experimental design was completely randomized in a 4 × 2 factorial scheme, with four replications. Four doses of phosphate fertilization (0, 100, 200, and 300 kg ha-1 P2O5) were studied, combined with two inoculation treatments with Bradyrhizobium japonicum (inoculated and not inoculated). The following variables were evaluated: plant height, stem diameter, nodules per plant, dry mass of nodules, dry mass of plant, dry mass of root, number of pods and number of grains per pod. Under greenhouse conditions and soil with good availability of phosphorus, there is no influence of the doses on the inoculation with Bradyrhizobium japonicum. Soils with good availability of phosphorus have low response to the application of phosphate fertilizer.

scholarly journals Competition between related strains of nodule bacteria and its influence on infection of the legume host

1941 ◽  
Vol 130 (858) ◽  
pp. 32-59 ◽  
Keyword(s):  
Growth Rate ◽  
Nitrogen Fixation ◽  
Root System ◽  
Short Duration ◽  
Pure Culture ◽  
The Other ◽  
Initial Growth ◽  
Nodule Bacteria ◽  
Long Period ◽  
Different Strains

The work deals with the behaviour of mixed strains of nodule bacteria towards each other and towards their legume host. It introduces the concept of dominance in competition between strains. This dominance is independent of degree of effectiveness as regards nitrogen fixation. Where tow strains of nodule bacteria are both present in the surroundings of their host's root system, active competition between them may cause the strain having the higher initial growth rate almost completely to check multiplication of the other strain outside the plant. This dominant strain will then be responsible for nearly all the nodules. In peas and soy beans, where growth of the root sysytem is rapid and of comparatively short duration, the nodule-producing capacity of the plant may be partially or wholly satisfied by the nodules produced within the first few weeks, so that further infection, whether by the same or by a different strain, is checked or inhibited. In clover, whose root system continues to grow over a long period, the first-formed nodules do not stop further nodules from being formed either by the same or by a different strain. There are large differences in the rates of appearance and final numbers of nodules produced by different strains supplied in pure culture, particularly with clover. The relative numbers of nodules produced by the two strains simultaneously applied to the roots is conditioned by the specific infectivity peculiar to each strain, unless some other factor, such as competition outside the plant, masks this effect.

scholarly journals STUDY OF SOYBEAN SEED MICROFLORA AS А ONE OF PROBABLE FACTORS OF BRADYRHIZOBIUM JAPONICUM DISTRIBUTION

2008 ◽  
Vol 6 ◽  
pp. 84-91
Author(s):  
D.V. Krutуlo
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Soybean Seed ◽  
Seed Storage ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Soybean Rhizobia ◽  
Soybean Nodule ◽  
Deep Layers

The results of studying of the soybean nodule bacteria presence in epiphytic and endophytic microflora of different grades of soybean seeds are presented. The soybean rhizobia was not revealed on the surface and in deep layers of soybean seeds. The soybean seed microbiota of investigated varieties is presented by several morphological types of bacteria and fungy. Their quantity depends on the period of seed storage.

scholarly journals THE GROWTH OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE IN THE MIXED CULTURE IN VITRO

2011 ◽  
Vol 13 ◽  
pp. 70-81
Author(s):  
S.F. Kozar ◽  
T.O. Usmanova ◽  
T.A. Zherebor
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Stress Factors ◽  
Lag Phase ◽  
Bacterial Cells ◽  
Nodule Bacteria ◽  
Initial Concentration ◽  
Soybean Nodule ◽  
Growth Activity

As a result of the conducted researches the substantial influence of azospirillium bacteria on the growth activity of nodule bacteria as comparing to the influence of Bradyrhizobium japonicum on growth of Azospirillum brasilense was found out. The greatest quantity of the studied bacteria was observed at average initial concentration (0,272 milliard/cm3) of bacterial cells of every kind at their initial ratio 1:1. It was shown that combined cultivation of soybean nodule bacteria and azospirillium bacteria reduces effect of stress factors on the growth of B. japonicum in the lag-phase.

scholarly journals THE EFFICIENCy OF DIFFERENT MEANS OF SOyBEAN INOCULATION wITH NODULE BACTERIA

2010 ◽  
Vol 10 ◽  
pp. 20-28
Author(s):  
S.M. Malichenko ◽  
V.K. Datsenko ◽  
P.M. Mamenko ◽  
S.Ya. Kots
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nitrogenase Activity ◽  
Soybean Seeds ◽  
Nodule Bacteria ◽  
Standard Strain ◽  
Bacterial Cultures ◽  
Tn5 Mutants ◽  
Soybean Plants ◽  
Production Strain

The efficiency of the presowing soybean inoculation with nodule bacteria at direct seeds bacterization or introduction of inoculum to the soil at presowing cultivation as well as the ability of rhizobia remained in the soil to the next year to form active symbioses with soybean plants was studied. The liquid bacterial cultures of production strain Bradyrhizobium japonicum 634b and three perspective Tn5-mutants of B. japonicum 646 were used. The introduction of the inoculum to soil was shown to be more efficient during both years of investigations as compared with the seeds inoculation which resulted in higher number of nodules formed, their nitrogenase activity and greater soybean seeds yield. Two of three studied Tn5-mutants had surpassed the standard strain by the efficiency indices.

scholarly journals The action of sodium nitrate upon the infection of Lucerne root-hairs by nodule bacteria

1936 ◽  
Vol 119 (815) ◽  
pp. 474-492 ◽  
Keyword(s):  
Nitrogen Fixation ◽  
Nitrate Nitrogen ◽  
Root Hairs ◽  
Ammonium Salts ◽  
Nodule Formation ◽  
Extensive Literature ◽  
Nodule Bacteria ◽  
Cultural Methods ◽  
The Subject ◽  
Sufficient Concentration

The fact that nitrates and ammonium salts harmfully affect nodule formation and the subsequent nitrogen fixation in legumes is well known, and the problem has formed the subject of an extensive literature, refer­ences to which are quoted by Giöbel (1926), by Fred, Baldwin, and McCoy (1932), and by Allison and Ludwig (1934) and need not be given here inextenso . The technical difficulties involved in analysing the action of nitrate upon the association of host and nodule organism are considerable; moreover, the great variation in cultural methods used has made it difficult to compare the results of different workers and has sometimes produced apparent discordance between them. The following facts appear, how­ever, to have been established: 1. Nitrates in sufficient concentration reduce the number of nodules formed and may even entirely inhibit their appearance. Doses of from 5 to 175 mg of nitrate nitrogen per litre have been found entirely to stop nodule formation in agar cultures of legumes (Hiltner, 1900; Flamand,1903-4; Marchal, 1901; Hills, 1918). In sand and soil cultures the inhibiting doses have been found to range from 21 to 488 mg of nitrogen per litre of soil moisture or of solution in the sand (Fred and Graul, 1916; J. K. Wilson, 1917; Hills,1918; Strowd,1920; Ohkawara, 1928). The highest of these figures in both agar and pot cultures are probably excessive, since they refer to initial doses, whereas nodule formation may sometimes have occurred when a weaker concentration, initially inhibitive, was further reduced by assimilation.

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