Correction of rat acute cholangitis using water-soluble C60 fullerene nanoparticles

A new side-chain C60-fullerene functionalized thiophene copolymer bearing tributylphosphine-substituted hexylic lateral groups was successfully synthesized by means of a fast and effective post-polymerization reaction on a regioregular ω-alkylbrominated polymeric precursor. The growth of the polymeric intermediate was followed by NMR spectrometry in order to determine the most convenient reaction time. The obtained copolymer was soluble in water and polar solvents and was used as a photoactive layer in single-material organic photovoltaic (OPV) solar cells. The copolymer photovoltaic efficiency was compared with that of an OPV cell containing a water-soluble polythiophenic homopolymer, functionalized with the same tributylphosphine-substituted hexylic side chains, in a blend with a water-soluble C60-fullerene derivative. The use of a water-soluble double-cable copolymer made it possible to enhance the control on the nanomorphology of the active blend, thus reducing phase-segregation phenomena, as well as the macroscale separation between the electron acceptor and donor components. Indeed, the power conversion efficiency of OPV cells based on a single material was higher than that obtained with the classical architecture, involving the presence of two distinct ED and EA materials (PCE: 3.11% vs. 2.29%, respectively). Moreover, the synthetic procedure adopted to obtain single material-based cells is more straightforward and easier than that used for the preparation of the homopolymer-based BHJ solar cell, thus making it possible to completely avoid the long synthetic pathway which is required to prepare water-soluble fullerene derivatives.

Download Full-text

Water-Soluble Pristine C60 Fullerene Inhibits Liver Alterations Associated with Hepatocellular Carcinoma in Rat

Pharmaceutics ◽

10.3390/pharmaceutics12090794 ◽

2020 ◽

Vol 12 (9) ◽

pp. 794

Author(s):

Halyna Kuznietsova ◽

Natalia Dziubenko ◽

Tetiana Herheliuk ◽

Yuriy Prylutskyy ◽

Eric Tauscher ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Growth ◽

Redox State ◽

Liver Enzymes ◽

Water Soluble ◽

C60 Fullerene ◽

P53 Expression ◽

Animal Survival ◽

Inhibit Cell Growth ◽

Glucose 6 Phosphate Dehydrogenase

Excessive production of reactive oxygen species is the main cause of hepatocellular carcinoma (HCC) initiation and progression. Water-soluble pristine C60 fullerene is a powerful and non-toxic antioxidant, therefore, its effect under rat HCC model and its possible mechanisms were aimed to be discovered. Studies on HepG2 cells (human HCC) demonstrated C60 fullerene ability to inhibit cell growth (IC50 = 108.2 μmol), to induce apoptosis, to downregulate glucose-6-phosphate dehydrogenase, to upregulate vimentin and p53 expression and to alter HepG2 redox state. If applied to animals experienced HCC in dose of 0.25 mg/kg per day starting at liver cirrhosis stage, C60 fullerene improved post-treatment survival similar to reference 5-fluorouracil (31 and 30 compared to 17 weeks) and inhibited metastasis unlike the latter. Furthermore, C60 fullerene substantially attenuated liver injury and fibrosis, decreased liver enzymes, and normalized bilirubin and redox markers (elevated by 1.7–7.7 times under HCC). Thus, C60 fullerene ability to inhibit HepG2 cell growth and HCC development and metastasis and to improve animal survival was concluded. C60 fullerene cytostatic action might be realized through apoptosis induction and glucose-6-phosphate dehydrogenase downregulation in addition to its antioxidant activity.

Download Full-text

A nanocomplex of C60 fullerene with cisplatin: design, characterization and toxicity

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.8.149 ◽

2017 ◽

Vol 8 ◽

pp. 1494-1501 ◽

Cited By ~ 16

Author(s):

Svitlana Prylutska ◽

Svitlana Politenkova ◽

Kateryna Afanasieva ◽

Volodymyr Korolovych ◽

Kateryna Bogutska ◽

...

Keyword(s):

Human Lymphocytes ◽

Annexin V ◽

Water Soluble ◽

C60 Fullerene ◽

Blast Transformation ◽

Force Microscopy ◽

Dna Strand ◽

Resting Lymphocytes ◽

Microscopy Techniques

The self-organization of C60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two compounds. The genotoxicity of С60 fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. The cytotoxicity of the mentioned compounds was estimated by Annexin V/PI double staining followed by flow cytometry. The results clearly demonstrate that water-soluble C60 fullerene nanoparticles (0.1 mg/mL) do not induce DNA strand breaks in normal and transformed cells. C60 fullerene in the mixture with Cis does not influence genotoxic Cis activity in vitro, affects the cell-death mode in treated resting human lymphocytes and reduces the fraction of necrotic cells.

Download Full-text

Highly Water-soluble and Surface Charge-tunable Fluorescent Fullerene Nanoparticles: Facile Fabrication and Cellular Imaging

Electrochimica Acta ◽

10.1016/j.electacta.2016.03.198 ◽

2016 ◽

Vol 201 ◽

pp. 220-227 ◽

Cited By ~ 8

Author(s):

Rongbin Xie ◽

Zifei Wang ◽

Hongtao Yu ◽

Zetan Fan ◽

Fanglong Yuan ◽

...

Keyword(s):

Surface Charge ◽

Cellular Imaging ◽

Water Soluble ◽

Fullerene Nanoparticles ◽

Facile Fabrication

Download Full-text

Evaluation of the Biodistribution of Serinolamide-Derivatized C60 Fullerene

Nanomaterials ◽

10.3390/nano10010143 ◽

2020 ◽

Vol 10 (1) ◽

pp. 143 ◽

Cited By ~ 1

Author(s):

Nicholas G. Zaibaq ◽

Alyssa C. Pollard ◽

Michael J. Collins ◽

Federica Pisaneschi ◽

Mark D. Pagel ◽

...

Keyword(s):

Carbon Nanoparticles ◽

Chelating Agent ◽

Water Soluble ◽

Emission Tomography ◽

C60 Fullerene ◽

Fullerene Cage ◽

Metal Chelating ◽

In Vivo Biodistribution ◽

Positron Emission

Carbon nanoparticles have consistently been of great interest in medicine. However, there are currently no clinical materials based on carbon nanoparticles, due to inconsistent biodistribution and excretion data. In this work, we have synthesized a novel C60 derivative with a metal chelating agent (1,4,7-Triazacyclononane-1,4,7-triacetic acid; NOTA) covalently bound to the C60 cage and radiolabeled with copper-64 (t1/2 = 12.7 h). Biodistribution of the material was assessed in vivo using positron emission tomography (PET). Bingel-Hirsch chemistry was employed to functionalize the fullerene cage with highly water-soluble serinolamide groups allowing this new C60 conjugate to clear quickly from mice almost exclusively through the kidneys. Comparing the present results to the larger context of reports of biocompatible fullerene derivatives, this work offers an important evaluation of the in vivo biodistribution, using experimental evidence to establish functionalization guidelines for future C60-based biomedical platforms.

Download Full-text

Synthesis and Complex Study of Water‐Soluble Polymer Derivatives of C60 Fullerene

Fullerenes Nanotubes and Carbon Nanostructures ◽

10.1081/fst-120027220 ◽

2005 ◽

Vol 12 (1-2) ◽

pp. 361-364 ◽

Cited By ~ 1

Author(s):

O. V. Ratnikova ◽

E. Yu. Melenevskaya ◽

N. P. Yevlampieva ◽

E. V. Tarassova ◽

V. N. Zgonnik

Keyword(s):

Water Soluble ◽

C60 Fullerene ◽

Soluble Polymer ◽

Water Soluble Polymer ◽

Complex Study ◽

Derivatives Of

Download Full-text

EFFECTS OF C60 FULLERENE NANOPARTICLES ON SOIL BACTERIA AND PROTOZOANS

Environmental Toxicology and Chemistry ◽

10.1897/07-375.1 ◽

2008 ◽

Vol 27 (9) ◽

pp. 1895 ◽

Cited By ~ 118

Author(s):

Anders Johansen ◽

Anette L. Pedersen ◽

Keld A. Jensen ◽

Ulrich Karlson ◽

Bjarne M. Hansen ◽

...

Keyword(s):

Soil Bacteria ◽

C60 Fullerene ◽

Fullerene Nanoparticles

Download Full-text

Nanoparticles C60 fullerene prevent reactive gliosis in retina of aged rats under hyperglycemia

Visnyk of Dnipropetrovsk University Biology medicine ◽

10.15421/021521 ◽

2015 ◽

Vol 6 (2) ◽

pp. 113-118

Author(s):

I. V. Prischepa ◽

O. G. Prokushenkova ◽

V. S. Nedzvetsky

Keyword(s):

Diabetic Retinopathy ◽

Glial Cells ◽

Glycosylated Hemoglobin ◽

Initial Period ◽

Protective Action ◽

Diabetic Rats ◽

Water Soluble ◽

Reactive Gliosis ◽

C60 Fullerene ◽

Diabetic Rat

Reactivation of glial cells, induced by metabolic disorders of glucose utilization and development of oxidative stress in retina under diabetes mellitus, is the key pathogenetic factor of diabetic retinopathy. Nanoparticles of C60 fullerene and some of their water-soluble derivates are known as one of the strongest antioxidants having neuroprotective effect in a number of pathologies and harmful influences. In the present study, for the first time, the effects of nanostructures of hydrated C60 fullerene (C60HyFn) on the expression and polypeptide composition of glial fibrillary acidic protein (GFAP) in retina of rats with streptozotocin (STZ)-induced diabetes have been evaluated. Using immunoblotting, 1.93-fold up-regulation of GFAP in diabetic rat retina as compared with control was shown, as a result of retinal glial cells reactivation induced by hyperglycemia. Increase in GFAP-immunolabeling associated with the reactive gliosis development in retina of diabetic rats was also confirmed by immuno-histochemical method. Consumption of C60HyFn solution (90 nM) as drinking water by diabetic rats for 12 weeks caused 1.51-fold decrease of GFAP level compared to untreated diabetic animals. In addition, C60HyFn caused statistically significant lowering of glycosylated hemoglobin concentration in blood serum of STZ-diabetic rats 1.58-fold. However, nanoparticles C60 did not affect neither insulin nor glucose levels in blood of diabetic rats. In conclusion, results obtained indicate that protective action of hydrated fullerene in the initial period of diabetic retinopathy of aged animals is realized through suppression of excessive activation of GFAP-positive retinal cells.

Download Full-text