scholarly journals "Dose – effect" calibration dependence by frequency of unstable chromosomic exchanges in human lymphocytes in acute gamma irradiation by 137Сs in low doses for biological dosimetry

2021 ◽  
Vol 22 (2) ◽  
pp. 167-173
Author(s):  
V.A. Kurochkina ◽  
◽  
L.K. Bezdrobna ◽  
T.V. Tsyganok ◽  
I.A. Khomych
Keyword(s):  
Gamma Irradiation ◽  
Human Lymphocytes ◽  
Dose Range ◽  
Dose Effect ◽  
Low Doses ◽  
Radiation Factor ◽  
Calibration Dependence ◽  
Zone Of Influence ◽  
Operation Duration

The calibration dependence of dicentrics and rings chromosomes with an accompanying fragment induction in human lymphocytes by in vitro 137Сs acute gamma irradiation of blood in the dose range of 0.09 - 1.0 Gy is presented. The application of the obtained calibration curve for estimation of “biological” doses for overexposed 9 persons from the contract staff of the Chornobyl SSE is shown. Their doses calculated by the frequency of dicentrics and centric rings with consideration of operation duration and mode in the zone of influence of the radiation factor, significantly exceeded the doses determined by the methods of physical dosimetry.

The Effects of Low Doses of Gamma Irradiation on the Ultrastructure ofNosema Apis in Vitro

1990 ◽  
Vol 29 (3) ◽  
pp. 165-171 ◽  
Author(s):  
T. P. Liu ◽  
T. A. Batchelor ◽  
R. J. Munn ◽  
J. M. Marston ◽  
C. L. Judson
Keyword(s):  
Gamma Irradiation ◽  
Low Doses

scholarly journals Comparative studies on the genotoxic activity of a new palladium (II) acidocomplex vs cisplatin in human blood lymphocytes

2013 ◽  
Vol 59 (1) ◽  
pp. 107-114 ◽  
Author(s):  
A.K. Grekhova ◽  
L.B. Gorbacheva ◽  
N.A. Ivanova ◽  
I.A. Efimenko ◽  
A.N. Osipov
Keyword(s):  
Human Lymphocytes ◽  
Dose Effect ◽  
Cross Linking ◽  
Oxygen Species ◽  
Comet Tail ◽  
Plateau Region ◽  
New Class ◽  
Genotoxic Activity ◽  
Anion Complexes

A comparative study on the genotoxic activity of cisplatin versus morfozol, the first representative of a new class of cation-anion complexes of palladium [AH] [PdCl ] (where A-methylmorpholine) has been performed using human lymphocytes in vitro. The results of the DNA-DNA cross-linking activity investigations showed that both compounds studied exhibited biphasic dose-effect relationship: a linear decrease in the DNA percent in the comet tail and the region of the "plateau". However, in the "plateau" region, morfozol reduced the DNA percent in the comet tail up to 6 times while cisplatin caused a 2-fold decrease only. Morfozol, like cisplatin, inducing DNA-protein cross-linking and generating reactive oxygen species, was more effective than cisplatin.

P02.02 Modeling of response to irradiation in recurrence glioblastoma’s cells culture

2021 ◽  
Vol 23 (Supplement_2) ◽  
pp. ii17-ii17
Author(s):  
N Antipina ◽  
A Belyashova ◽  
G Pavlova ◽  
A Nikolaeva ◽  
E Savchenko ◽  
...  
Keyword(s):  
Experimental Data ◽  
Proliferative Activity ◽  
Dose Range ◽  
Combined Treatment ◽  
Mathematical Expression ◽  
Dose Effect ◽  
Proliferating Cells ◽  
Culture Cells ◽  
Effect Curve

Abstract BACKGROUND Radiosensitivity of glioblastoma (GB) cells of local relapses may be markedly different from the primary tumor. Optimal doses and regimes of re-irradiation GB recurrence is not determined yet. MATERIAL AND METHODS GO1 primary GB cell culture was obtained during removal of a recurrent tumor after combined treatment, including irradiation of the surgical bed. Сell’s culture was irradiated by photon beams with energy 6 MeV and dose rate 600 MU/min. Irradiation performed in 1, 3 and 5 fractions, by 10 different doses for each regime. The dose range was determined experimentally for one fraction (5–250 Gy); for other regimes it was calculated according to the biological equivalent dose conception (3 fractions: 5–450 Gy, 5 fractions: 5–550 Gy). The proliferative activity of cells was investigate by MTT test. The results were normalized to the control. Dose-effect curves were plotted for each irradiation regime.The experimental data were approximated by calculated curves obtained by selecting the optimal parameters of the LQ-model and it’s modification. RESULTS Irradiation of GO1 by 1 fraction with the dose 5–250 Gy, causes a slow decrease in proliferative activity, which reaches a minimum value of 23% at 150 Gy and then remains constant. After irradiation by 3 fractions, proliferative activity of the GO1 gradually decrease only at a total dose over 120 Gy and reaches 37% after 450 Gy. When GO1 was irradiated in 5 fractions, a similar dose-effect curve was obtained, gradual decrease was observed to a value of 52% in the range of 250–500 Gy. Thus, the experimental dose-effect curves for irradiation of recurrence GB cells for 3 and 5 fractions have the appreciable “shoulder”, which could be explained by increased radioresistance. When approximating the experimental data by fitting the parameters of the LQ-model, the use of α/β = 8 provided the slope of the curve, close to the experimental data. For reflecting the “shoulder” an additional summand was introduced into the mathematical expression for the number of proliferating cells - a 105 Gy for 3 fractions and 255 Gy for 5 fractions. CONCLUSION Modified LQ-model could be used for an adequate mathematical description of the effectiveness of fractionated irradiation in relapsed GB culture cells in vitro. It’s necessary to introduce a summand into the formula that determines the formation of a “shoulder” on the dose-effect curve for this. The research was supported financially by RFBR (Project No. 18-29-01061).

Three-Color FISH Method: Dose-Effect Curves for Translocations in Peripheral Blood Lymphocyte Cultures after Gamma-Irradiation In Vitro

2021 ◽  
Vol 65 (5) ◽  
pp. 12-20
Author(s):  
V. Nugis ◽  
G Snigiryova ◽  
E. Lomonosova ◽  
M. Kozlova ◽  
V. Nikitina
Keyword(s):  
Gamma Irradiation ◽  
Peripheral Blood ◽  
Cytogenetic Analysis ◽  
Venous Blood ◽  
Dna Probes ◽  
Dose Effect ◽  
Color Version ◽  
Fish Method ◽  
60Co Source

Purpose: Plotting dose-effect curves for translocations identified using the tricolor FISH method based on the results of cytogenetic analysis of cultures of peripheral blood lymphocytes of healthy donors after in vitro gamma irradiation. Material and methods: Venous blood was obtained from three donors (2 men and 1 woman aged from 28 to 41 years) and subjected to in vitro gamma irradiation from a 60Co source at doses of 0.10; 0.15; 0.25; 0.35; 0.50; 0.75; 1.00; 1.50; 2.00 and 3.00 Gy at 37 ° C (dose rate 0.5 Gy / min). For tricolor FISH staining, two different sets of DNA probes were used for chromosome pairs 1, 4, 12 and 2, 3, 8. Metaphases with a quasi-diploid number of chromosomes (40-46) and a complete set of all FISH-stained chromosomes, taking into account their total length, were selected for analysis. Differentiation of stable and unstable cells was also carried out. In the cytogenetic analysis, traditional terminology was used with the designation of translocations as reciprocal (complete, two-sided), non-reciprocal (terminal, incomplete, or unilateral), or interstitial. Results: The obtained numerical data were used to statistically compare the frequencies of FISH-recorded translocations when using different sets of DNA probes, when calculating of chromosome aberrations were in all (unstable and stable) and stable metaphase cells, when comparing of the frequencies of FISH-recorded translocations and dicentrics, and assessing of the contribution of the level of translocations between FISH-stained chromosome pairs in the total translocation frequency. The plotted dose-effect curves generally corresponded to the linear-quadratic form. Conclusion: Dose dependences obtained for translocations using two different selected tricolor sets of DNA probes did not differ statistically significantly. At the same time, cytogenetic analysis of only stable metaphase cells revealed a tendency to register lower levels of translocations than when analyzing all cells (unstable and stable ), at the highest doses of 2 and 3 Gy. The levels of dicentrics formed with the participation of FISH-stained chromosomes were significantly lower than the number of observed translocations. The quantitative contribution of translocations between FISH-stained pairs of chromosomes turned out to be very low, which clearly does not contribute to an increase in the sensitivity of the FISH method of retrospective dose estimation as compared to its one-color version. At the same time, the three-color FISH-staining makes it possible to identify such variants of chromosomal rearrangements that are not recorded using the one-color FISH method.

scholarly journals Cytotoxicity and genotoxicity of low doses of mercury chloride and methylmercury chloride on human lymphocytes in vitro

2005 ◽  
Vol 38 (6) ◽  
pp. 901-907 ◽  
Author(s):  
L.C. Silva-Pereira ◽  
P.C.S. Cardoso ◽  
D.S. Leite ◽  
M.O. Bahia ◽  
W.R. Bastos ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
Mercury Chloride ◽  
Low Doses ◽  
Methylmercury Chloride

Effect of Low Doses and High Homeopathic Potencies in Normal and Cancerous Human Lymphocytes: An In Vitro Isopathic Study

2006 ◽  
Vol 12 (5) ◽  
pp. 421-427 ◽  
Author(s):  
Chantal Wälchli ◽  
Stephan Baumgartner ◽  
Madeleine Bastide
Keyword(s):  
Human Lymphocytes ◽  
Low Doses

Gene Expression Profiles in Human Lymphocytes Irradiated In Vitro with Low Doses of Gamma Rays

2007 ◽  
Vol 168 (6) ◽  
pp. 650 ◽  
Author(s):  
Ana L. Fachin ◽  
Stephano S. Mello ◽  
Paula Sandrin-Garcia ◽  
Cristina M. Junta ◽  
Eduardo A. Donadi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gamma Rays ◽  
Expression Profiles ◽  
Human Lymphocytes ◽  
Gene Expression Profiles ◽  
Low Doses

Preliminary in vitro investigation into the use of alkaline elution assay for the biomonitoring of humans exposed to genotoxic agents

1995 ◽  
Vol 14 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Therese Leroy ◽  
Dominique Lison ◽  
Robert Lauwerys
Keyword(s):  
Dna Damage ◽  
Gamma Irradiation ◽  
Human Lymphocytes ◽  
Cell Types ◽  
Dna Lesions ◽  
Alkaline Elution ◽  
Repair Capacity ◽  
In Vitro Investigation ◽  
Genotoxic Agents

1 This in vitro study was undertaken as a preliminary approach before assessing whether the alkaline elution assay can be applied to peripheral blood lymphocytes (PBL) for the monitoring of humans exposed to genotoxic agents such as polycyclic aromatic hydrocarbons (PAH). We have compared in vitro, with the aid of the alkaline elution assay, the formation and the repair of DNA single- strand breaks (ssb) induced by different genotoxic agents [gamma-irradiation, ethyl methanesulfonate (EMS), benzo<a>pyrene diol epoxide (BPDE)] on quiescent and PHA-stimulated human lymphocytes and on a fibroblast cell line. 2 Gamma-irradiation (4 Gy) induced an equivalent amount of DNA ssb in the three cell types. On the other hand, after treatment with EMS (10mM) and BPDE (50μM), a higher production of DNA ssb was observed in replicat ing cells (PHA-stimulated lymphocytes and fibroblasts) when compared with quiescent lymphocytes. 3 After gamma-irradiation, all cell types repaired more than 65% of ssb within 1 h. After treatment with EMS, we noted a deficient DNA repair capacity in quiescent lym phocytes in comparison with replicating cells. In all cell types treated with BPDE, more breaks were observed after a 2 h repair period than immediately after treatment, demonstrating the involvement of a slow repair mecha nism after BPDE treatment. 4 Several conclusions can be drawn from this pilot study, (i) when assessing in vitro the induction and the repair of DNA lesions induced by chemicals, it seems rea sonable to test both non-replicating and replicating cells since their response may be different; (ii) in view of the relative persistence of DNA damage induced in vitro by BPDE in resting lymphocytes, chronic exposure to PAH could give rise to a certain accumulation of DNA damage in coke oven workers lymphocytes. Further studies will be necessary to determine whether these damages could be detected by alkaline elution.

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