scholarly journals Development of an early-stage femoral head necrosis rabbit model using methylprednisolone and Complete Freund's Adjuvant

2017 ◽  
Vol 4 (11) ◽  
pp. 1749
Author(s):  
Lan Thi Phi ◽  
Ha Thi -Ngan Le ◽  
Thuy Thi -Thanh Dao ◽  
Khanh Dinh-Van Nguyen ◽  
Khanh Hong-Thien Bui ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bone Marrow ◽  
Body Weight ◽  
Femoral Head ◽  
Mononuclear Cells ◽  
Early Stage ◽  
Rabbit Model ◽  
Normal Group ◽  
Femoral Head Necrosis ◽  
Freund's Adjuvant

Introduction: Pathological animal models provide the foundation for developing new methods for disease treatment. This research aims to establish a rabbit model of femoral head necrosis. Methods: Osteonecrosis of the femoral head (ONFH) was induced in rabbits by using methylprednisolone (MPS) combined with Complete Freund's Adjuvant (CFA). New Zealand White rabbits were divided into two groups. ONFH group (n=10) was given an intramuscular injection of 0.5 mg/kg CFA and 40 mg/kg methylprednisolone. Normal group (n=6) received normal saline at the same location and same volume as those in ONFH group. The efficiency of the ONFH rabbit model was assessed at week 7 after the last injection. Body weight was detected, and the histological structure of head femoral and bone were assessed by H&E staining. The empty lacunae were counted. Cartilage degeneration was evaluated using image analysis software. Blood vessel density was assessed after ink artery infusion. The cell cycle of bone marrow-derived mononuclear cells was analyzed by flow cytometry. Results: The results showed that there was no difference in body weight changes of rabbits between the two groups. However, the bone morphology and cartilage surface of the femoral head showed abnormalities in the ONFH group. The percentage of empty osteocyte lacunae was significantly higher in ONFH group than normal group. Chondrocyte degeneration and fibrocartilage expression were observed in the ONFH group. Compared to the normal group, the ONFH group had less ink-stained blood vessels. However, the fraction of bone marrow-derived mononuclear cells in S phase and G2/M phase of the cell cycle was significantly increased in the ONFH group. Conclusion: Thus, CFA combined with MPS for 7 weeks can be used to establish an early-stage femoral head necrosis model in rabbits.

scholarly journals ID: 1071 Establishing an early-stage femoral head necrosis model of rabbit using methylprednisolone and Complete Freund's Adjuvant

2017 ◽  
Vol 4 (S) ◽  
pp. 153
Author(s):  
Lan Thi Phi ◽  
Thuy Thi-Thanh Dao ◽  
Khanh Dinh-Van Nguyen ◽  
Phuc Van Pham ◽  
Khanh Hong-Thien Bui ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bone Marrow ◽  
Body Weight ◽  
Femoral Head ◽  
Early Stage ◽  
Rabbit Model ◽  
Femoral Head Necrosis ◽  
Group A ◽  
Freund's Adjuvant ◽  
Group B

Background: Pathological animal models provide the foundation for developing new methods for treating. This research aims to establish a rabbit model of femoral head necrosis. Osteonecrosis of the femoral head (ONFH) was induced in rabbits by using methylpresnisolone combined with Complete Freund's Adjuvant (CFA). New Zealand White rabbits were divided into two groups. Group A (n=10) was given an intramuscular injection of 0.5 mg/kg (CFA) and 40mg/kg methylprednisolone (MPS). Group B (n=6) was received normal saline at the same location and same volume as those in Group A. The efficiency of ONFH rabbit model was assessed at 7 weeks after the last injection. Body weight was weighed. The histological structure of head femoral and bone were deteded by H&E staining. The empty lacuna was counted. Cartilage degeneration was evaluated using image analysis software. Blood vessel density was assessed after ink artery infusion. The cell cycle of bone marrow-derived mononuclear cells was analyzed by flow cytometry. The results showed that there was no difference in body weight change of rabbits between two groups. However, the bone morphology and cartilage surface of femoral head were abnormalities at group A. The percentage of empty osteocyte lacunae were significantly higher in Group A than Group B. Chondrocyte degeneration and fibrocartilage expression were observed at Group A. Compare to group B, Group A had less ink-stained blood vessels. Moreover, the fraction of bone marrow-derived mononuclear at S phase and G2/M phase of the cell cycle was significantly decreased in group A. Thus, CFA combined with MPS can  be used to establishing an early-stage femoral head necrosis model of rabbit.

scholarly journals Comparative Dose of Intracarotid Autologous Bone Marrow Mononuclear Therapy in Chronic Ischemic Stroke in Rats

2021 ◽  
Vol 9 (A) ◽  
pp. 233-243
Author(s):  
Feda Makkiyah ◽  
Wismaji Sadewo ◽  
Rahmah Nurrizka
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Ischemic Stroke ◽  
Test Scores ◽  
Mononuclear Cells ◽  
Marrow Cell ◽  
Optimum Dose ◽  
Infarct Zone ◽  
Positive Effects ◽  
Cylinder Test

Research on chronic ischemic stroke is limited. One of the more promising approaches showing positive effects in the acute stage is mononuclear bone marrow cell therapy. This research may be the first which presents data about the optimum dose of bone marrow mononuclear cells (BM-MNCs) for chronic ischemic stroke in rats and discusses factors influencing recovery in the chronic stage. We performed temporary middle cerebral artery occlusion (MCAO)  procedures on the rats which were then randomly assigned to one of two experimental groups in which they were given either low or high doses of autologous BM-MNCs  (5 million or 10 million cells per kg body weight). Rat brains were fixed for HE, CD31, and doublecortin staining for analysis of the effects. Rat behavior was assessed weekly using the cylinder test and a modified neurological severity score (NSS) test. In the four weeks prior to administration of BM-MNC, cylinder test scores improved to near normal, and NSS test scores improved moderately. The infarct zone decreased significantly (p <0,01),  there was an improvement in angiogenesis (p = 0.1590) and a significant improvement in neurogenesis (p <0,01). Reduction of the infarct zone was associated with a higher dose whereas both higher and lower doses were found to have a similar effect on improving angiogenesis, and neurogenesis. Recovery was superior after twelve weeks compared with the recovery assessment at eight weeks. In conclusion, a dose of 10 million cells was more effective than a dose of 5 million cells per kg body weight for reducing the infarct zone and ameliorating neurogenesis. There was an improvement of histopathological parameters associated with the longer infarct period.

scholarly journals The effects of a functionally-graded scaffold and bone marrow-derived mononuclear cells on steroid-induced femoral head osteonecrosis

Biomaterials ◽  
2018 ◽  
Vol 187 ◽  
pp. 39-46 ◽  
Author(s):  
Masahiro Maruyama ◽  
Akira Nabeshima ◽  
Chi-Chun Pan ◽  
Anthony W. Behn ◽  
Timothy Thio ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Femoral Head ◽  
Mononuclear Cells ◽  
Functionally Graded ◽  
Femoral Head Osteonecrosis

scholarly journals The efficacy and safety of core decompression for the treatment of femoral head necrosis: a systematic review and meta-analysis

2019 ◽  
Vol 14 (1) ◽  
Author(s):  
Kun-chi Hua ◽  
Xiong-gang Yang ◽  
Jiang-tao Feng ◽  
Feng Wang ◽  
Li Yang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Femoral Head ◽  
Radiographic Progression ◽  
Meta Analysis ◽  
Core Decompression ◽  
Femoral Head Necrosis ◽  
Significant Difference ◽  
Autologous Bone ◽  
Necrosis Of Femoral Head ◽  
Rate Of Success

Abstract Background Core decompression (CD) is an important method for the treatment of osteonecrosis of the femoral head (ONFH). Few articles investigate the influence of core decompression on outcomes of ONFH. This study was carried out to observe the safety and effectiveness of core decompression in the treatment of ONFH. Methods A comprehensive literature search of databases including PubMed, Embase, and Cochrane Library was performed to collect the related studies. The medical subject headings used were “femur head necrosis” and “Core decompression.” The relevant words in title or abstract included but not limited to “Osteonecrosis of the Femoral Head,” “femoral head necrosis,” “avascular necrosis of femoral head,” and “ischemic necrosis of femoral head.” The methodological index for nonrandomized studies was adopted for assessing the studies included in this review. Results Thirty-two studies included 1865 patients (2441 hips). Twenty-one studies (1301 hips) using Ficat staging standard, 7 studies (338hips) using Association Research Circulation Osseous (ARCO) staging standard, and University of Pennsylvania system for staging avascular necrosis (UPSS) staging criteria for 4 studies (802 hips). All the studies recorded the treatment, 22 studies (1379 hips) were treated with core decompression (CD) alone, and 7 studies (565 hips) were treated with core decompression combined with autologous bone (CD Autologous bone). Nine subjects (497 hips) were treated with core decompression combined with autologous bone marrow (CD Marrow). Twenty-seven studies (2120 hips) documented the number of conversion to total hip replacement (THA), and 26 studies (1752hips) documented the number of radiographic progression (RP). Twenty-one studies recorded the types of complications and the number of cases, a total of 69 cases. The random-effect model was used for meta-analysis, and the results showed that the overall success rate was 65%. The rate of success showed significant difference on the outcomes of different stages. The rate of success, conversion to THA, and radiographic progression showed significant difference on the outcomes of ONFH using different treatments. Conclusions Core decompression is an effective and safe method of treating ONFH. The combined use of autologous bone or bone marrow can increase the success rate. For advanced femoral head necrosis, the use of CD should be cautious. High-quality randomized controlled trials and prospective studies will be necessary to clarify the effects of different etiology factors, treatments, and postoperative rehabilitation. Until then, the surgeon can choose core decompression to treat ONFH depending on the patient’s condition. Level of evidence I Meta-analysis

Early Results of Core Decompression and Autologous Bone Marrow Mononuclear Cells Instillation in Femoral Head Osteonecrosis

2012 ◽  
Vol 27 (5) ◽  
pp. 679-686 ◽  
Author(s):  
Ramesh Kumar Sen ◽  
Sujit Kumar Tripathy ◽  
Sameer Aggarwal ◽  
Neelam Marwaha ◽  
Ratti Ram Sharma ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Femoral Head ◽  
Mononuclear Cells ◽  
Core Decompression ◽  
Autologous Bone Marrow ◽  
Bone Marrow Mononuclear Cells ◽  
Femoral Head Osteonecrosis ◽  
Early Results ◽  
Autologous Bone
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