Investigation of RARg Signaling in Human Growth-Plate Chondrocytes

2021 ◽  
Author(s):  
Joshua M. Abzug ◽  
Hongying Tian ◽  
Masatake Matsuoka ◽  
Danielle A. Hogarth ◽  
Casey M. Codd ◽  
...  
Keyword(s):  
Growth Plate ◽  
Human Growth ◽  
Growth Plate Chondrocytes

scholarly journals Cellular reactions to biodegradable magnesium alloys on human growth plate chondrocytes and osteoblasts

2013 ◽  
Vol 38 (4) ◽  
pp. 881-889 ◽  
Author(s):  
Karin Pichler ◽  
Tanja Kraus ◽  
Elisabeth Martinelli ◽  
Patrick Sadoghi ◽  
Giuseppe Musumeci ◽  
...  
Keyword(s):  
Growth Plate ◽  
Magnesium Alloys ◽  
Human Growth ◽  
Growth Plate Chondrocytes ◽  
Biodegradable Magnesium

The synthesis of type X collagen by bovine and human growth-plate chondrocytes

1991 ◽  
Vol 99 (3) ◽  
pp. 641-649 ◽  
Author(s):  
A. Marriott ◽  
S. Ayad ◽  
M.E. Grant
Keyword(s):  
Growth Plate ◽  
Type I Collagen ◽  
Polyacrylamide Gel Electrophoresis ◽  
Human Growth ◽  
Type I ◽  
Collagen Gels ◽  
Type X Collagen ◽  
Growth Plate Chondrocytes ◽  
Growth Plate Cartilage ◽  
Disulphide Bonds

Chondrocytes were isolated from bovine growth-plate cartilage and cultured within type I collagen gels. A major collagen with chains of Mr 59,000, decreasing to 47,000 on pepsinization, was synthesized and identified as type X collagen. This collagen was cleaved at two sites by mammalian collagenase, resulting in a major triple-helical fragment with chains of Mr 32,000. The species of Mr 59,000, 47,000 and 32,000 were not detected by SDS-polyacrylamide gel electrophoresis before reduction, indicating the presence of disulphide bonds within the triple helix. In contrast, similar biosynthetic studies with human growth-plate cartilage in organ culture, indicated that human type X collagen does not contain disulphide bonds. A polyclonal antiserum was raised to bovine type X collagen and used in immunolocalization studies to provide direct evidence for the association of type X collagen with the hypertrophic chondrocytes in both bovine and human growth plates during development.

The effect of mechanical stretch stress on the differentiation and apoptosis of human growth plate chondrocytes

2016 ◽  
Vol 53 (2) ◽  
pp. 141-148 ◽  
Author(s):  
Keming Sun ◽  
Fangna Liu ◽  
Junjian Wang ◽  
Zhanhao Guo ◽  
Zejuan Ji ◽  
...  
Keyword(s):  
Growth Plate ◽  
Human Growth ◽  
Mechanical Stretch ◽  
Growth Plate Chondrocytes

scholarly journals Genes uniquely expressed in human growth plate chondrocytes uncover a distinct regulatory network

BMC Genomics ◽  
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Bing Li ◽  
Karthika Balasubramanian ◽  
Deborah Krakow ◽  
Daniel H. Cohn
Keyword(s):  
Growth Plate ◽  
Regulatory Network ◽  
Human Growth ◽  
Growth Plate Chondrocytes

scholarly journals The Short Stature Homeodomain Protein SHOX Induces Cellular Growth Arrest and Apoptosis and Is Expressed in Human Growth Plate Chondrocytes

2004 ◽  
Vol 279 (35) ◽  
pp. 37103-37114 ◽  
Author(s):  
Antonio Marchini ◽  
Tiina Marttila ◽  
Anja Winter ◽  
Sandra Caldeira ◽  
Ilaria Malanchi ◽  
...  
Keyword(s):  
Short Stature ◽  
Growth Plate ◽  
Growth Arrest ◽  
Human Growth ◽  
Cellular Growth ◽  
Homeodomain Protein ◽  
Growth Plate Chondrocytes

Expression of matrix metalloproteinases in human growth plate chondrocytes is enhanced at high levels of mechanical loading

2013 ◽  
Vol 95-B (4) ◽  
pp. 568-573 ◽  
Author(s):  
K. Pichler ◽  
V. Herbert ◽  
B. Schmidt ◽  
E. E. Fischerauer ◽  
A. Leithner ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Growth Plate ◽  
Mechanical Loading ◽  
Human Growth ◽  
Growth Plate Chondrocytes

scholarly journals Epigenetic profiling of growth plate chondrocytes sheds insight into regulatory genetic variation influencing height

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Michael Guo ◽  
Zun Liu ◽  
Jessie Willen ◽  
Cameron P Shaw ◽  
Daniel Richard ◽  
...  
Keyword(s):  
Growth Plate ◽  
Human Growth ◽  
Causal Variant ◽  
Open Chromatin ◽  
Growth Plate Chondrocytes ◽  
Growth Plates ◽  
Binding Motifs ◽  
Biologically Relevant ◽  
Open Chromatin Region ◽  
Reporter Assays

GWAS have identified hundreds of height-associated loci. However, determining causal mechanisms is challenging, especially since height-relevant tissues (e.g. growth plates) are difficult to study. To uncover mechanisms by which height GWAS variants function, we performed epigenetic profiling of murine femoral growth plates. The profiled open chromatin regions recapitulate known chondrocyte and skeletal biology, are enriched at height GWAS loci, particularly near differentially expressed growth plate genes, and enriched for binding motifs of transcription factors with roles in chondrocyte biology. At specific loci, our analyses identified compelling mechanisms for GWAS variants. For example, at CHSY1, we identified a candidate causal variant (rs9920291) overlapping an open chromatin region. Reporter assays demonstrated that rs9920291 shows allelic regulatory activity, and CRISPR/Cas9 targeting of human chondrocytes demonstrates that the region regulates CHSY1 expression. Thus, integrating biologically relevant epigenetic information (here, from growth plates) with genetic association results can identify biological mechanisms important for human growth.

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