Lactulosuria in the Neonate: A Preliminary Report

PEDIATRICS ◽  
1965 ◽  
Vol 35 (2) ◽  
pp. 340-342
Author(s):  
JOYCE D. GRYBOSKI ◽  
JOHN J. BOEHM
Keyword(s):  
High Voltage ◽  
Preliminary Report ◽  
Milk Formula ◽  
Breast Fed ◽  
Electrophoresis Technique ◽  
High Voltage Electrophoresis

Urines from 121 neonates were examined for sugars by a high-voltage electrophoresis technique. Lactosuria and maltosuria occurred in 15.4% and 7.7% respectively of infants taking evaporated milk formula. Lactosuria occurred in 21.6% of infants receiving Similac and in 28.6% of the breast-fed infants. Lactulose, a sugar not previously described in urines of normal newborns, was present in the urine of 15.4% of infants taking evaporated milk and in 38.6% of infants taking Similac.

High-voltage electrophoresis of choline and its esters

1974 ◽  
Vol 89 (1) ◽  
pp. 96-98 ◽  
Author(s):  
Sheila E. Brooker ◽  
Keith J. Harkiss
Keyword(s):  
High Voltage ◽  
High Voltage Electrophoresis

scholarly journals Isolation, Characterization and Pathology of the Toxin From a Microcystis Aeruginosa (= Anacystis Cyanea) Bloom

1978 ◽  
Vol 31 (3) ◽  
pp. 209 ◽  
Author(s):  
TC Elleman ◽  
IR Falconer ◽  
ARB Jackson ◽  
MT Runnegar
Keyword(s):  
High Voltage ◽  
Algal Bloom ◽  
Lethal Dose ◽  
Acid Precipitation ◽  
Blue Green Alga ◽  
Laboratory Mice ◽  
Ammonium Sulphate Fractionation ◽  
Tyrosine D ◽  
Simple Amino Acid ◽  
High Voltage Electrophoresis

The nature of the toxicity of a bloom of blue-green alga, M. aeruginosa (= Anacystis cyanea), that occurred in a man-made lake was investigated. Crude algal bloom extracts were toxic to laboratory mice when injected intraperitoneaIIy. The lethal dose (LDlOO) of these extracts was 15-30 mg of lyophilized algal bloom per kilogram body weight. The toxin was purified by a procedure that included ammonium sulphate fractionation, solvent extraction, acid precipitation, Sephadex G25 and DEAE-Sephadex chromatography, and high-voltage electrophoresis at pH 6�5. The preparation gave a single spot on high-voltage electrophoresis at pH 9�0, had no free amino group, and was characterized by a simple amino acid composition of equimolar quantities of L-methionine, L-tyrosine, D-alanine, D-glutamic acid, erythro fi-methyl aspartic acid and methylamine.

A paper wetness probe for high voltage electrophoresis

1975 ◽  
Vol 65 (1-2) ◽  
pp. 89-92
Author(s):  
George Ibars ◽  
John I. Peterson ◽  
J.S. Finlayson
Keyword(s):  
High Voltage ◽  
High Voltage Electrophoresis

ISOLATION AND STRUCTURE OF URIDINE NUCLEOTIDE-PEPTIDES FROM AEROBACTER CLOACAE NRC 492

1963 ◽  
Vol 41 (1) ◽  
pp. 1065-1072 ◽  
Author(s):  
R. A. Anwar ◽  
C. Roy ◽  
R. W. Watson
Keyword(s):  
Lactic Acid ◽  
Amino Acid ◽  
Lithium Chloride ◽  
Paper Chromatography ◽  
High Voltage ◽  
Chloride Solutions ◽  
Uridine Nucleotide ◽  
Ethanol Extracts ◽  
Dowex 1 ◽  
High Voltage Electrophoresis

Four closely related uridine nucleotide-peptides isolated from ethanol extracts of penicillin-treated Aerobacter cloacae NRC 492 have been partially characterized. Fractionation of dialyzed extracts on Dowex-1-Cl columns with aqueous lithium chloride solutions, precipitation of the nucleotide-peptides with methanol–acetone, and further separation by paper chromatography and high voltage electrophoresis yielded (a) UDP-GNAc-lact.L-ala.D-glu.meso-dap.D-ala,* (b) UDP-GNAc-lact.L-ala.D-glu.meso-dap, (c) UDP-GNAc-lact.L-ala.D-glu., and (d) UDP-GNAc-lactic acid. The amino acid isomers were identified by micro-enzymic and chromatographic methods.

Comparative studies of the exotoxins of Pseudomonas glycinea and Pseudomonas phaseolicola

1978 ◽  
Vol 56 (3) ◽  
pp. 282-287 ◽  
Author(s):  
Randall S. Murch ◽  
Suresh S. Patil
Keyword(s):  
High Voltage ◽  
Membrane Filter ◽  
Thin Layers ◽  
Gel Chromatography ◽  
Ornithine Carbamoyltransferase ◽  
Aspartate Carbamoyltransferase ◽  
Leaf Tissues ◽  
Soybean Leaf ◽  
Soybean Leaves ◽  
High Voltage Electrophoresis

A sensitive and quantitative bioassay, based on the ability of the exotoxin of Pseudomonas glycinea to inhibit ornithine carbamoyltransferase of bean, was employed in a comparative study of the P. glycinea and P. phaseolicola toxins using high-voltage electrophoresis, thin-layer chromatography, ultrafiltration, and Sephadex-gel chromatography. The P. glycinea toxin (glytoxin) has an elution volume/void volume (Ve/V0) ratio different from that of phaseotoxin when chromatographed on Sephadex G-25. Glytoxin passes through a membrane filter with an exclusion limit of 500 daltons whereas phaseotoxin does not. High voltage electrophoresis in buffers of different pH values showed that glytoxin, like phaseotoxin, migrates as an anion but shows greater mobility than phaseotoxin. Both toxins degrade on thin layers of silica gel. Glytoxin induces chlorosis in bean and soybean leaf tissues, and like phaseotoxin, is an inhibitor of ornithine carbamoyltransferase but not of aspartate carbamoyltransferase. Glytoxin is presumably responsible for the accumulation of ornithine which was observed in soybean leaves infected with P. glycinea. Our studies show that glytoxin and phaseotoxin are similar but not identical.

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