Increased sensitivity of detection for high-voltage electrophoresis of phosphorus compounds in salicylate buffer with boric acid

The nature of the toxicity of a bloom of blue-green alga, M. aeruginosa (= Anacystis cyanea), that occurred in a man-made lake was investigated. Crude algal bloom extracts were toxic to laboratory mice when injected intraperitoneaIIy. The lethal dose (LDlOO) of these extracts was 15-30 mg of lyophilized algal bloom per kilogram body weight. The toxin was purified by a procedure that included ammonium sulphate fractionation, solvent extraction, acid precipitation, Sephadex G25 and DEAE-Sephadex chromatography, and high-voltage electrophoresis at pH 6�5. The preparation gave a single spot on high-voltage electrophoresis at pH 9�0, had no free amino group, and was characterized by a simple amino acid composition of equimolar quantities of L-methionine, L-tyrosine, D-alanine, D-glutamic acid, erythro fi-methyl aspartic acid and methylamine.

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Lactulosuria in the Neonate: A Preliminary Report

PEDIATRICS ◽

10.1542/peds.35.2.340 ◽

1965 ◽

Vol 35 (2) ◽

pp. 340-342

Author(s):

JOYCE D. GRYBOSKI ◽

JOHN J. BOEHM

Keyword(s):

High Voltage ◽

Preliminary Report ◽

Milk Formula ◽

Breast Fed ◽

Electrophoresis Technique ◽

High Voltage Electrophoresis

Urines from 121 neonates were examined for sugars by a high-voltage electrophoresis technique. Lactosuria and maltosuria occurred in 15.4% and 7.7% respectively of infants taking evaporated milk formula. Lactosuria occurred in 21.6% of infants receiving Similac and in 28.6% of the breast-fed infants. Lactulose, a sugar not previously described in urines of normal newborns, was present in the urine of 15.4% of infants taking evaporated milk and in 38.6% of infants taking Similac.

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27. QUANTITATIVE IMMUNOCHEMICAL DETERMINATION OF DEGRADATION PRODUCTS OF FIBRINOGEN AND/OR FIBRIN WITH HIGH VOLTAGE ELECTROPHORESIS

Scandinavian Journal of Haematology ◽

10.1111/j.1600-0609.1971.tb02010.x ◽

2009 ◽

Vol 8 (S13) ◽

pp. 215-217

Author(s):

Jan-Erik Niléhn

Keyword(s):

High Voltage ◽

Degradation Products ◽

Immunochemical Determination ◽

High Voltage Electrophoresis

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A paper wetness probe for high voltage electrophoresis

Analytical Biochemistry ◽

10.1016/0003-2697(75)90494-7 ◽

1975 ◽

Vol 65 (1-2) ◽

pp. 89-92

Author(s):

George Ibars ◽

John I. Peterson ◽

J.S. Finlayson

Keyword(s):

High Voltage ◽

High Voltage Electrophoresis

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ISOLATION AND STRUCTURE OF URIDINE NUCLEOTIDE-PEPTIDES FROM AEROBACTER CLOACAE NRC 492

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y63-119 ◽

1963 ◽

Vol 41 (1) ◽

pp. 1065-1072 ◽

Cited By ~ 3

Author(s):

R. A. Anwar ◽

C. Roy ◽

R. W. Watson

Keyword(s):

Lactic Acid ◽

Amino Acid ◽

Lithium Chloride ◽

Paper Chromatography ◽

High Voltage ◽

Chloride Solutions ◽

Uridine Nucleotide ◽

Ethanol Extracts ◽

Dowex 1 ◽

High Voltage Electrophoresis

Four closely related uridine nucleotide-peptides isolated from ethanol extracts of penicillin-treated Aerobacter cloacae NRC 492 have been partially characterized. Fractionation of dialyzed extracts on Dowex-1-Cl columns with aqueous lithium chloride solutions, precipitation of the nucleotide-peptides with methanol–acetone, and further separation by paper chromatography and high voltage electrophoresis yielded (a) UDP-GNAc-lact.L-ala.D-glu.meso-dap.D-ala,* (b) UDP-GNAc-lact.L-ala.D-glu.meso-dap, (c) UDP-GNAc-lact.L-ala.D-glu., and (d) UDP-GNAc-lactic acid. The amino acid isomers were identified by micro-enzymic and chromatographic methods.

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Comparative studies of the exotoxins of Pseudomonas glycinea and Pseudomonas phaseolicola

Canadian Journal of Botany ◽

10.1139/b78-035 ◽

1978 ◽

Vol 56 (3) ◽

pp. 282-287 ◽

Cited By ~ 2

Author(s):

Randall S. Murch ◽

Suresh S. Patil

Keyword(s):

High Voltage ◽

Membrane Filter ◽

Thin Layers ◽

Gel Chromatography ◽

Ornithine Carbamoyltransferase ◽

Aspartate Carbamoyltransferase ◽

Leaf Tissues ◽

Soybean Leaf ◽

Soybean Leaves ◽

High Voltage Electrophoresis

A sensitive and quantitative bioassay, based on the ability of the exotoxin of Pseudomonas glycinea to inhibit ornithine carbamoyltransferase of bean, was employed in a comparative study of the P. glycinea and P. phaseolicola toxins using high-voltage electrophoresis, thin-layer chromatography, ultrafiltration, and Sephadex-gel chromatography. The P. glycinea toxin (glytoxin) has an elution volume/void volume (Ve/V0) ratio different from that of phaseotoxin when chromatographed on Sephadex G-25. Glytoxin passes through a membrane filter with an exclusion limit of 500 daltons whereas phaseotoxin does not. High voltage electrophoresis in buffers of different pH values showed that glytoxin, like phaseotoxin, migrates as an anion but shows greater mobility than phaseotoxin. Both toxins degrade on thin layers of silica gel. Glytoxin induces chlorosis in bean and soybean leaf tissues, and like phaseotoxin, is an inhibitor of ornithine carbamoyltransferase but not of aspartate carbamoyltransferase. Glytoxin is presumably responsible for the accumulation of ornithine which was observed in soybean leaves infected with P. glycinea. Our studies show that glytoxin and phaseotoxin are similar but not identical.

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An Aorta Intima Inhibitor of Platelet Aggregation

10.1055/s-0039-1682696 ◽

1977 ◽

Author(s):

A.duP. Heyns ◽

C.J. Badenhorst ◽

F.P. Retief

Keyword(s):

Platelet Aggregation ◽

High Voltage ◽

Vessel Wall ◽

Prostaglandin Synthesis ◽

Human Aorta ◽

Intravascular Thrombosis ◽

Wall Interaction ◽

High Voltage Electrophoresis

The interaction of platelets and the vessel wall is of importance in the pathogenesis of intravascular thrombosis and atherosclerosis. We have previously described the presence of an inhibitor of platelet aggregation and a low Km ADPase in aorta intima extracts. The inhibitor was further characterized.Human aorta intimas were homogenized, centrifuged and supernatants used. Platelet aggregation was measured in a aggregometer.ADPase activity was measured by incubating 14C-ADP with intima, separating metabolites by high voltage electrophoresis and quantitated.Aggregation induced by ADP, collagen, Ristocetin and polylysine was inhibited by the intima extract. The inhibition was still evident in aspirin treated platelets. The inhibitor is present in the supernatant of 100 000g centrifuged extracts. This inhibitor appears not to be ADPase or the recently described prostacyclin (PGX) :- it is stable and not destroyed by incubation at 37°C, blocking of prostaglandin synthesis by pre-incubation of the intima with indomethacin has no effect on inhibitor activity.ADPase is inactivated by KCN whereas the inhibitor is not.The inhibitor of platelet aggregation and to a lesser extent ADPase, in the intima may be important regulators of platelet-vessel wall interaction.

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O-Methyl sugars in lipopolysaccharides of Rhodospirillacea. Identification of 3-O-methyl-d-mannose in Rhodopseudomonas viridis and of 4-O-methyl-d-xylose and 3-O-methyl-6-deoxy-d-talose in Rhodopseudomonas palustris respectively

Biochemical Journal ◽

10.1042/bj1350293 ◽

1973 ◽

Vol 135 (2) ◽

pp. 293-297 ◽

Cited By ~ 20

Author(s):

Jürgen Weckesser ◽

Hubert Mayer ◽

Inge Fromme

Keyword(s):

Mass Spectrometry ◽

Paper Chromatography ◽

High Voltage ◽

Optical Rotation ◽

Rhodopseudomonas Palustris ◽

Rhodopseudomonas Viridis ◽

Gram Negative ◽

Optical Configuration ◽

Alditol Acetates ◽

High Voltage Electrophoresis

1. This paper deals with the identification of three O-methyl sugars in lipopolysaccharides isolated from strains of the Gram-negative photosynthetic family Rhodospirillaceae. In addition to the previously described 3-O-methyl-l-xylose, a second O-methyl sugar was encountered in the lipopolysaccharide of Rhodopseudomonas viridis F, namely 3-O-methyl-d-mannose. The lipopolysaccharides of two strains of Rhodopseudomonas palustris (strain 1e5 and 8/1) contain two O-methylsugars, 4-O-methyl-d-xylose and 3-O-methyl-6-deoxy-d-talose (d-acovenose). 4-O-Methyl-d-xylose, but not 3-O-methyl-6-deoxy-d-talose, could be identified in the lipopolysaccharides of the strains K/1 and 2/2 of the same species. 2. The O-methyl sugars described in this communication were isolated by paper chromatography and identified by g.l.c., paper chromatography, high-voltage electrophoresis and mass spectrometry. Besides the genuine sugars, their alditol acetates and their demethylated (parental) forms were investigated. Optical rotation measurements and, in one case, enzymic reactions were used to establish the optical configuration of the sugars under investigation.

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