scholarly journals Experimental study of tropism in cultivated canine coronavirus in the small intestine of puppies

2019 ◽  
Vol 10 (4) ◽  
pp. 489-496
Author(s):  
L. Goralskii ◽  
N. Radzіkhovsky ◽  
О. Dyshkant ◽  
O. Dunaievska ◽  
I. Sokulskiy
Keyword(s):  
Small Intestine ◽  
Inclusion Bodies ◽  
Morphological Changes ◽  
Pathological Process ◽  
Pathogenic Role ◽  
Histological Changes ◽  
Canine Coronavirus ◽  
Acidic Proteins ◽  
Heterologous Cell

The varying extents of natural disease induced by coronavirus in dogs are not completely clear because the pathogenesis of coronavirus enteritis is not studied sufficiently. In this study, based on the results of clinical, virological, morphological and histochemical studies, we determined the pathogenic role of coronavirus in infected dogs using experimental infection, per os, of isolated canine coronavirus (Nick) with titer of infectious activity equaling 4.8 ± 0.04 lg TCID50/cm, cultivated on heterologous cell cultures. This allowed us to determine, supplement, and generalize the data on pathogenesis of the disease and determine the histological changes in the small intestine, where the initial replication of the pathogen takes place. It was found that lesions and the pattern of the pathomorphological changes (destruction, necrosis and edema of the stroma of the villi, lysis of the cytoplasm, deformation of the enterocyte nuclei) in the small intestine of experimentally infected dogs depend on the development of the pathological process related not only to the changes in histoarchitectonics of the wall of the intestine, but also to tension of the histochemical statics, and obviously the dynamic of the cells (accumulation of the main and acidic proteins in enterocytes’ cytoplasm, hypersecretion of the mucus by goblet cells, decrease of Schiff iodine acid-positive substances in the enterocytes’ cytoplasm, formation of basophilous inclusion bodies), which leads to disorders in metabolic processes in the organism of infected dogs as a response to the virus infection. The examined dogs were found to have morphological changes in the small intestine similar to those in spontaneously infected animals. During the action of coronavirus, the contacts between the enterocytes become damaged, which leads to inhibition of the protective functions of the intestine. At the same time, the pathological process in the experimentally infected animals developed rapidly and had an acute course. Thus, coronavirus enteritis as a separate disease is practically unobserved in field conditions, which makes microscopic survey of the pathogenic impact of the latter on the organism of dogs impossible. Therefore, experimental mono-infection allows a detailed study to be conducted of pathomorphological changes of the initial place of the reproduction of the virus – the small intestine affected by coronavirus enteritis.

Maternal passive smoking during pregnancy and foetal developmental toxicity. Part 2: histological changes

1999 ◽  
Vol 18 (4) ◽  
pp. 257-264 ◽  
Author(s):  
Ed Nelson ◽  
Carla Goubet-Wiemers ◽  
Yuanjian Guo ◽  
Karin Jodscheit
Keyword(s):  
Growth Retardation ◽  
Passive Smoking ◽  
Morphological Changes ◽  
Developmental Toxicity ◽  
Smoking History ◽  
Pregnant Rats ◽  
Histological Changes ◽  
Smoking During Pregnancy ◽  
Sidestream Smoke

1 Evidence has been accumulating on the growth suppressing effects of maternal passive smoking on foetus. Reviewing all literature released during the last two decades and screening for all possible variables such as previous smoking history, maternal age and weight gain, parity and length of gestation, placental weight, and diet, we found no reason to doubt the role of passive smoking during pregnancy in the induction of growth retardation. However, no literature indicates whether these birthweight deficits might hint at other possible hidden abnormalities in tissues. To verify this question, we performed an experiment on rats. 2 We have already reported that pups born to rats with previous exposure to cigarette' sidestream smoke during pregnancy showed a significant and dosedependent growth retardation.1 Those pregnant rats were exposed each in a 150 dm3 glass chamber to diluted sidestream smoke of either 1, 2, 3 or 4 commercial blond filter brand cigarettes during either first, second or third week of pregnancy. We have selected a part of each group of pups at random and examined for possible histological changes of lung, liver, stomach, kidney and intestinal tissues. 3 Compared to controls, lung tissues of newborns of smoke exposed mothers showed an enhanced incidence of apoptosis, mesenchymal changes, and hyperplasia of bronchial muscles. Pronounced abnormal changes in haematopoiesis and proliferation of bile duct cells were the most variations from norm observed in liver tissues of exposed pups. Immature glomeruli of kidney, epithelhypoplasia of stomach, and hypoplasia of intestinal villi were common among newborns of exposed mothers than among controls. 4 These results indicate that passive smoking upon pregnancy causes abnormal morphological changes in internal tissues of newborns. At present, we can draw no conclusion as to whether these histological changes will result in functional malformations or possibly late effects, although they should be expected.

scholarly journals The role of small intestine in pathogenesis of common variable immune deficiency

2018 ◽  
Vol 90 (2) ◽  
pp. 43-46
Author(s):  
L M Krums ◽  
A I Parfenov ◽  
R B Gudkova ◽  
S V Bykova ◽  
E A Sabelnikova
Keyword(s):  
Immune Deficiency ◽  
Small Intestine ◽  
Blood Serum ◽  
Clinical Picture ◽  
Digestive System ◽  
Morphological Structure ◽  
Pathological Process ◽  
Common Variable Immune Deficiency ◽  
Common Variable

The article presents the results of examination of 32 patients with common variable immune deficiency (barn) with involvement in the pathological process of the digestive system. The features of the clinical picture, the content of immunoglobulins in the blood serum, morphological structure of the mucosa of small intestine as well as treatment. Special attention is paid to the small intestine in the pathogenesis of the barn.

scholarly journals МОРФОЛОГІЧНІ ОСОБЛИВОСТІ МІОКАРДІАЛЬНОЇ ФОРМИ ПАРВОВІРУСНОЇ ІНФЕКЦІЇ СОБАК

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 16-20
Author(s):  
B. Borysevych ◽  
V. Lisova ◽  
A. Shats’ka
Keyword(s):  
Heart Failure ◽  
Inclusion Bodies ◽  
Morphological Changes ◽  
Clinical Signs ◽  
Pathological Process ◽  
Morphological Features ◽  
Post Mortem ◽  
Eosinophilic Inclusion ◽  
Stromal Edema ◽  
Circulatory Disorders

Presented results of studying of morphological features of the pathological process at the cardial form of parvovirus infection of dogs. Identified the main criteria for it post–mortem diagnostics.It is shown that based on the results of pathological studies myocarditis of the parvovirus etiology clearly differentiate from other deadly diseases that affect puppies.In puppies aged 1 to 4 weeks, who died suddenly without clinical signs and biochemical changes of blood, which are characteristic for myocarditis, was detected pathomorphological signs of myocardial forms of parvovirus infection of dogs.Histological examination revealed the characteristic morphological changes: 1) lymphocytic (purulent) myocarditis 2) eosinophilic inclusion–bodies in nuclei of cardiomyocytes 3) stromal edema 4) granular dystrophy of cardiomyocytes 5) necrosis of cardiomyocytes with the destruction of the muscle fibers of the myocardium.Also nonspecific but constant morphological features, that is caused by circulatory disorders and heart failure was venous hyperemia of the liver, kidney and pulmonary edema.

scholarly journals Pathogenicity of intestinal flagellates in mice

1969 ◽  
Vol 3 (1) ◽  
pp. 71-77 ◽  
Author(s):  
A. Sebesteny
Keyword(s):  
Small Intestine ◽  
Clinical Investigation ◽  
Pathogenic Role ◽  
And Control

The results of the clinical investigation and control of a murine condition associated with heavy Hexamita muris infestation of the small intestine revealed its similarity to murine giardiasis, and suggested a possible pathogenic role of Hexamita muris in mice.

THE ROLE OF ENTEROSCOPY IN THE PREOPERATIVE DIAGNOSIS OF BLEEDING GASTROINTESTINAL STROMAL TUMORS OF THE SMALL INTESTINE

2019 ◽  
Author(s):  
DE Seleznev ◽  
EV Ivanova ◽  
OI Yudin ◽  
EV Tikhomirova ◽  
MA Shumikhina ◽  
...  
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Stromal Tumors ◽  
Preoperative Diagnosis ◽  
Stromal Tumors

The pathogenic role of surfactant protein B in formation of bronchopulmonary pathology in children

2017 ◽  
pp. 66-71
Author(s):  
O. Yablon ◽  
◽  
N. Zaichko ◽  
O. Mazulov ◽  
Z.I. Rossokha ◽  
...  
Keyword(s):  
Surfactant Protein ◽  
Pathogenic Role ◽  
Surfactant Protein B ◽  
Protein B

Pathogenic role of CCL2/MCP-1 in scleroderma

10.2741/2875 ◽  
2008 ◽  
Vol 13 (13) ◽  
pp. 2686 ◽  
Author(s):  
Toshiyuki Yamamoto
Keyword(s):  
Pathogenic Role
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