scholarly journals Formación de embriones somáticos a partir de semillas inmaduras en Sorghum bicolor variedad CIAP 132-R

2014 ◽  
Vol 20 (2) ◽  
Author(s):  
Mayelín Rodríguez Urquiza ◽  
Rafael Gómez Kosky ◽  
Silvio De Jesús Mratínez ◽  
Mileydi Pons Corona ◽  
Martha Pérez Peralta ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Plant Regeneration ◽  
Sorghum Bicolor ◽  
Somatic Embryos ◽  
Culture Medium ◽  
Callus Formation ◽  
Embryo Formation ◽  
Sorghum Variety ◽  
Immature Seeds ◽  
First Time

<p class="Body">Existen varios protocolos de regeneración de plantas vía embriogénesis somática de <em>Sorghum bicolor</em> (L.) Moench, sin embargo los porcentajes de formación de callos con estructuras embriogénicas y regeneración de plantas son bajos. Es por ello que esta investigación tuvo como objetivo generar embriones somáticos en sorgo rojo variedad  CIAP 132-R. Se ensayaron diferentes concentraciones de 2,4-D para la formación de callos, así como tres concentraciones de ácido ascórbico para eliminar la exudación de compuestos fenólicos por el explante. También para la formación de los embriones somáticos a partir de los callos se evaluaron diferentes concentraciones de 2,4-D y 6-BAP. El mayor porcentaje de formación de callos (57.5 %) se alcanzó con 18.1 µM de 2,4-D. Con la adición al medio de cultivo de 50.0 mg.l<sup>-1</sup> de ácido ascórbico fue posible eliminar los compuestos fenólicos en el explante y en el medio de cultivo, además permitió incrementar el porcentaje de formación de callos con estructuras embriogénicas hasta un 95 %. El número mayor de embriones somáticos por callo se alcanzó en el medio de cultivo con concentraciones de 4,52 µM de 2,4-D, combinada con 2,22 µM de 6-BAP. Por primera vez, se logró la formación eficiente de embriones somáticos a partir de los callos obtenidos de semillas inmaduras  germinadas como explante inicial en la variedad CIAP 132-R.</p><p class="Body"><strong>ABSTRACT</strong></p><p class="Body">Several protocols of plant regeneration via somatic embryogenesis from <em>Sorghum bicolor</em> (L.) Moench have been development, however the percentage of calluses with embryogenic structures and plant regeneration are low. Therefore this study aimed to generate somatic embryos in red sorghum variety CIAP 132-R. Different concentrations of 2,4-D for callus formation, and three concentrations of ascorbic acid to remove phenolics exudation were assayed by explant. For the formation of embryos different concentrations of 2,4-D and 6-BAP were evaluated. The highest percentage of callus formation (57.5 %) was achieved with 18.1 µM 2,4-D. With the addition to the culture medium of 50.0 mg.l<sup>-1</sup> of ascorbic acid was possible to eliminate the phenolic compounds in the explant and in the culture medium; also it allows increasing the percentage of calluses with embryogenic structures up to 95 %. The highest number of somatic embryos per callus was achieved with a reduction in the culture medium of 2,4-D to 4.52 µM in combination with 2.22 µM 6-BAP. For the first time, the efficiency of somatic embryo formation was obtained from the freshly germinated sprouts of immature seeds as initial explant CIAP 132-R.</p>

Study on Tissue Culture of Sweet Broad Pea

2014 ◽  
Vol 644-650 ◽  
pp. 5407-5410
Author(s):  
Hui Fang Chi
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Culture Medium ◽  
Callus Formation ◽  
Formation Rate ◽  
Adventitious Bud ◽  
Experimental Basis ◽  
Research Results

s. The cotyledons, Internodes, leaves and stems of sweet broad pea were studied on tissue culture. Research results show that: The ability of different explants for callus formation and adventitious bud differentiation in different culture medium is different. The callus formation rate and sprouting rate of Internodes is significantly higher than other explants, which is a ideal material for tissue culture. The callus formation rate of Internodes was 100% in MS +BA1.0 mg/L+NAA 1.0 mg/L and MS+ 2, 4-D 0.5 mg/L; The bud differentiation is best at the medium of MS+ 6-BA 2 mg/L, which reached 86.7%; the rooting rate was 83.3% at the medium of MS+ NAA 3mg/L. The study provides a experimental basis for further study on the plant regeneration in the sweet broad pea.

scholarly journals Plant Regeneration through Somatic Embryogenesis from Leaf Sheath Derived Callus of Sugarcane (Saccharum officinarum L.) var. Isd -16

2011 ◽  
Vol 21 (2) ◽  
pp. 143-149 ◽  
Author(s):  
Mohashweta Roy ◽  
M. Hossain ◽  
A. Biswas ◽  
M. K. Biswas ◽  
R. Islam
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Saccharum Officinarum ◽  
Leaf Sheath ◽  
Coconut Water ◽  
Friable Callus ◽  
Light Yellow ◽  
Half Strength

Leaf sheath explants of an indigenous variety Isd-16 of sugarcane (Saccharum officinarum L.) produced light yellow friable callus after culturing on to MS with 2,4-D (2 - 4 mg/l) and NAA (3 - 5 mg/l) singly. Callus formation was the maximum on MS + 3 mg/l 2,4-D. Callus underwent embryogenesis producing huge number of somatic embryos when subcultured on MS with 15 - 30 mg/l             L-proline, 3 mg/l 2,4-D + 5 - 10% coconut water (v/v) and 3 mg/l 2,4-D + 10% CW  (v/v) + 300 - 500 mg/l CH. L-proline significantly enhanced somatic embryo-genesis and 25 mg/l L-proline in MS was the best culture medium formulation. Most of the somatic embryos germinated and developed plantlets after 1 - 2 weeks of incubation in proline-supplimented medium. On the other hand, maturation and germination of embryos were achieved on half-strength MS with or without 0.25 - 1.0 mg/l L-proline, and 5% coconut water (v/v). Somatic embryos derived plantlets were then successfully transferred to natural condition through successive phages of acclimation.   Key words: Plant regeneration, Leaf sheath, Somatic embryogenesis, Sugarcane   D. O. I. 10.3329/ptcb.v21i2.10237   Plant Tissue Cult. & Biotech. 21(2): 143-149, 2011 (December)

scholarly journals Plant regeneration through somatic embryogenesis from calli derived from leaf bases of Laurus nobilis L. (Lauraceae)

2015 ◽  
Vol 24 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Ahmad H Al Gabbiesh ◽  
M Ghabeish ◽  
I H ◽  
M Kleinwächter ◽  
D Selmar
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Root Formation ◽  
Callus Formation ◽  
Germplasm Conservation ◽  
Laurus Nobilis ◽  
The Media ◽  
Regenerated Plantlets ◽  
Culture Technology

Somatic embryogenesis was induced in embryo culture on half MS medium supplemented with NAA (8 mg/l) as the sole plant growth regulator after incubation of the media in the refrigerator at 4°C for two weeks to promote callus induction and somatic embryogenesis in Laurus nobilis. Both embryogenetic calli and somatic embryos were induced in the above selected medium. Embryo growth and development were stimulated by separation of embryos successfully from embryo clusters and transferred onto fresh half MS. Among the selected explants, only leaf bases were found to respond actively to plant regeneration, especially in inducing callus formation and in sustaining faster callus growth. Root formation of regenerated plantlets tended to decrease with time on regeneration media. Overall, 75% of the plantlets derived from the callus survived in the greenhouse; and they all grew to phenotypically normal plants. This procedure will enable the use of regeneration tissue culture technology for germplasm conservation of L. nobilis, a plant of high medicinal and commercial value.Plant Tissue Cult. & Biotech. 24(2): 213-221, 2014 (December)

Callus formation and plant regeneration from somatic embryos of oat (Avena sativa L.)

1991 ◽  
Vol 10 (5) ◽  
Author(s):  
P. Bregitzer ◽  
W.R. Bushnell ◽  
H.W. Rines ◽  
D.A. Somers
Keyword(s):  
Plant Regeneration ◽  
Avena Sativa ◽  
Somatic Embryos ◽  
Callus Formation

Somatic Embryogenesis From Styles of Different Cultivars of Citrus limon (L.) Burm

1994 ◽  
Vol 42 (5) ◽  
pp. 587 ◽  
Author(s):  
FD Pasquale ◽  
F Carimi ◽  
FG Crescimanno
Keyword(s):  
Somatic Embryos ◽  
Culture Medium ◽  
Callus Formation ◽  
Basal Medium ◽  
Citrus Limon ◽  
Naphthaleneacetic Acid ◽  
Malt Extract ◽  
Ms Medium ◽  
Culture Initiation ◽  
Embryogenic Response

Plants were regenerated from styles of three cultivars of lemon (Citrus limon (L.) Burm.), 'Monachello', 'Lunario' and 'Femminello Santa Teresa'. Styles and stigmas were excised from flowers, and cultured on Murashige and Skoog (MS) basal medium. Callus formation occurred from the style base 2 weeks after culture initiation and was significantly (P < 0.01) greater in immature flowers growing on BAP supplemented medium. Somatic embryos appeared 8 weeks later. The addition of BAP in the culture medium increased significantly (P < 0.01) the embryogenic response of the style, and the best regeneration occurred on MS basal medium supplemented with 13.3 μM 6-benzylaminopurine (BAP) and 146 mM sucrose. Once embryos were transferred to MS medium supplemented with 500 mg L-1 malt extract and 0.27 μM α-naphthaleneacetic acid (NAA), they developed into plantlets. After 5 or 6 months of continuous subculturing, the embryogenic callus became habituated and new embryos were still arising.

scholarly journals DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM IMMATURE OVULES IN YOOZA(CITRUS JUNOS SIEB. ET TANAKA)

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 694c-694
Author(s):  
Sung-Do Oh ◽  
Won-Seob Song ◽  
Man-Sang Lee
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryo ◽  
Somatic Embryos ◽  
Direct Somatic Embryogenesis ◽  
Malt Extract ◽  
Banding Patterns ◽  
Embryo Formation ◽  
Citrus Junos

From one week through 7 weeks after artificial pollination, immature ovules of yooza(Citrus junos Sieb. et Tanaka) were excised and cultured in vitro on MT media. Even though there was only a little difference in percentage of somatic embryo formation depending upon the time of excision, immature ovules of 4-week-old showed the highest ratio of somatic embryo formation without callus outgrowth. Various growth regulators or other stimulators were added to the MT media to increase the somatic embryogenesis, In general, BAP was more effective than 2,4-D for somatic embryo formation and the combinations of 0.01mg/l 2,4-D and 0,01 or 0.1mg/l BAP were particularly effective in stimulating somatic embryo formation. When 500mg/l malt extract was added to the medium, the percentage of somatic embryo formation increased reaching as high as 86.7%. Plant regeneration from somatic embryos reached to 66.7% on the medium containing 1.0mg/l zeatin. Isozyme banding patterns were also analyzed to confirm the variations of characteristics of the plantlets derived from direct somatic embryos.

scholarly journals REGENERATION OF CHINESE JUJUBE(ZIZYPHUS JUJUBA MILLER) FROM IMMATURE COTYLEDONS AND SEGMENTS OF SEEDLING

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 698f-698
Author(s):  
Sung-Do Oh ◽  
Won-Seob Song
Keyword(s):  
Somatic Embryos ◽  
Leaf Blade ◽  
Callus Formation ◽  
Ms Medium ◽  
Ethylene Synthesis ◽  
Chinese Jujube ◽  
Embryo Formation ◽  
Immature Cotyledons ◽  
Zizyphus Jujuba ◽  
Cobalt And Nickel

Immature cotyledons within nucleoulus of young fruits, 40-50 days old, were cultured on various media containing different concentrations of plant growth regulators. MS medium was most effective for callus formation, but cytokinins added to MS media containing 1.0 mg/l 2,4-D inhibited callus formation. Combination of 2,4-D and BAP was more effective than 2,4-D or BAP alone for somatic embryo formation from callus. Highest percent of somatic embryogenesis was observed on MS medium supplemented with 1.0mg/l 2,4-D and 50% sucrose. Cobalt and nickel, inhibitors of ethylene synthesis, reduced significantly the number of somatic embryos. Plantlets germinated freely from somatic embryos on MS medium containing 1.0mg/l zeatin and 0.01mg/1 2,4-D. Segments of hypocotyl, cotyledon and leaf blade were observed for callus formation, somatic enbryogenesis and plant regeneration. Shoots were directly differentiated from the callus induced from segments of hypocotyl on MS medium containing 0.1mg/l 2,4-D after 4 weeks of culture.

scholarly journals Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hyun-A Woo ◽  
Seong Sub Ku ◽  
Eun Yee Jie ◽  
HyeRan Kim ◽  
Hyun-Soon Kim ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regeneration System ◽  
Embryogenic Cell ◽  
Embryogenic Callus Formation

AbstractTo establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature zygotic embryos were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). At higher concentrations of 2,4-D (over 2 mg/L), the frequency of embryogenic callus formation declined significantly. The total number of somatic embryos development was highest with the 3% (w/v) sucrose treatment, which was found to be the optimal concentration for somatic embryo formation. Activated charcoal (AC) and 6-benzyladenine (BA) significantly increased the frequency of plantlet conversion from somatic embryos, but gibberellic acid (GA3) had a negative effect on plantlet conversion and subsequent development from somatic embryos. Even though the cell suspension cultures were maintained for more than 1 year, cell aggregates from embryogenic cell suspension cultures were successfully converted into normal somatic embryos with two cotyledons. To our knowledge, this is the first successful report of a plant regeneration system of E. alatus via somatic embryogenesis. Thus, the embryogenic cell line and plant regeneration system established in this study can be applied to mass proliferation and production of pharmaceutical metabolite in E. alatus.

scholarly journals Effect of Gelling Agent and Selective Sub-culturing on Hyperhydricity in Anther-Derived Coconut Embryos

CORD ◽  
2011 ◽  
Vol 27 (2) ◽  
pp. 12
Author(s):  
P I P Perera
Keyword(s):  
Plant Regeneration ◽  
Culture Medium ◽  
Basal Medium ◽  
Gelling Agent ◽  
Embryo Maturation ◽  
Liquid Culture Medium ◽  
Embryo Formation ◽  
Maturation Medium ◽  
The Media ◽  
Regeneration Efficiency

Experiments were conducted to compare the liquid medium with the media solidified with agar or phytagel. Selective subculturing and use of the embryo maturation medium supplemented with higher concentration of phytagel (0.5%; w/v) were also tested for reducing the vitrified embryos. Modified Eeuwens Y3 medium was used as the basal medium. By culturing the anthers on the medium solidified with phytagel (0.25%; w/v), direct embryo formation (86.7%) and embryo conversion (21.5%) were significantly increased. Plant regeneration efficiency of anther derived embryos or calluses developed in the liquid culture medium was extremely low (2.4%). Vitrification was further reduced by incorporating 0.5% (w/v) phytagel into the embryo maturation medium. Highest plant regeneration efficiency was obtained by exposing the embryos to 0.5% (w/v) phytagel for 21 days, which reduced vitrification by 42%. Furthermore, selective subculturing of the embryos was effective for reducing vitrification.

Sign in / Sign up

Export Citation Format

Share Document