scholarly journals Eficacia antiparasitaria in vitro del extracto de quebracho (Schinopsis balansae) sobre larvas infectantes de Haemonchus contortus de ovinos

2021 ◽  
Vol 68 (3) ◽  
Author(s):  
D. Vergara ◽  
F. Sagüés ◽  
E. J. Späth ◽  
M. M. Lloberas ◽  
C. A. Saumell ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
A Posteriori ◽  
Schinopsis Balansae ◽  
Los Grupos

El objetivo fue evaluar in vitro la eficacia del extracto de quebracho (Bioquina®), rico en taninos condensados, en el control de H. contortus de ovinos, dadas las evidencias de que los taninos condensados provenientes del extracto de quebracho (Schinopsis spp.) pueden reducir la excreción de huevos, la fecundidad de las hembras parásitas y la carga de parásitos nematodos gastrointestinales adultos en rumiantes. Se utilizó el test de inhibición de migración larval (IML) a 3 concentraciones diferentes (5 mg/ml, 15 mg/ml y 30 mg/ml) sobre larvas infectantes de H. contortus susceptibles a todos los grupos químicos. El efecto se analizó mediante un análisis de varianza y para la estimación de las diferencias entre grupos (a posteriori) se usó la prueba LSD Fisher. El test in vitro demostró que el extracto de quebracho evaluado redujo aproximadamente entre un 74% y un 80% la migración larval a las concentraciones de entre 5 mg/ml y 30 mg/ml. Del análisis de varianza surgen diferencias significativas entre tratamientos (p = 0,0494). Al realizar la prueba de comparación de medias se evidenciaron diferencias significativas (p < 0,05) entre los promedios de migración a las diluciones de 5 mg/ml y 15 mg/ml y de 5 mg/ml y 30 mg/ml, mientras que no se detectaron diferencias significativas entre la dilución de 15 mg/ml y 30 mg/ml. Estos resultados señalaron que el extracto de quebracho, a las diluciones evaluadas, presentó actividad antihelmíntica sobre larvas L3 susceptibles de H. contortus; sin embargo, se requiere ampliar los estudios in vivo en ovinos.

scholarly journals In vitro and in vivo action of Piptadenia viridiflora (Kunth) Benth against Haemonchus contortus in sheep

2016 ◽  
Vol 223 ◽  
pp. 43-49 ◽  
Author(s):  
F. Morais-Costa ◽  
G.A. Bastos ◽  
A.C.M. Soares ◽  
E.G.L. Costa ◽  
V.O. Vasconcelos ◽  
...  
Keyword(s):  
Haemonchus Contortus

Kinetics of exsheathment of infective ovine and bovine strongylid larvae in vivo and in vitro

Parasitology ◽  
2002 ◽  
Vol 125 (1) ◽  
pp. 65-70 ◽  
Author(s):  
H. HERTZBERG ◽  
U. HUWYLER ◽  
L. KOHLER ◽  
St REHBEIN ◽  
M. WANNER
Keyword(s):  
Haemonchus Contortus ◽  
Buffer System ◽  
Bicarbonate Concentration ◽  
Infective Larvae ◽  
Nylon Mesh ◽  
Mesh Bags ◽  
Sheep Rumen ◽  
Kinetics Of

The aim of the study was to investigate the longitudinal changes of exsheathment of ovine and bovine 3rd-stage strongylid larvae in an artificial rumen (RUSITEC) and to compare the results with in vivo data obtained from rumen-fistulated sheep. Infective larvae were incubated in nylon mesh bags in the sheep rumen or the RUSITEC apparatus for periods of 1, 6 and 12 h, respectively. The 12 h exsheathment rates in the rumen and the RUSITEC apparatus (in parentheses) were as follows: Haemonchus contortus: 100% (100%), Ostertagia circumcincta: 100% (76%), O. leptospicularis: 100% (100%), O. ostertagi: 53% (59%), Trichostrongylus axei: 100% (100%), T. colubriformis: 37% (36%), Cooperia curticei: 94% (76%), C. oncophora: 95% (89%), Nematodirus filicollis: 0% (N.D.), N. spathiger: 11% (15%), N. battus: 7% (5%), Oesophagostomum venulosum: 17% (9%), Chabertia ovina: 7% (2%), Dictyocaulus filaria: 1% (N.D.). Larvae of Nematodirus spp. and T. colubriformis showed a quick rise of the exsheathment rate 2 h after transfer into the abomasum. These results confirm that exsheathment generally occurs in the part of the gastrointestinal tract immediately anterior to the habitat of the adult parasite. The overall similar course of exsheathment in both systems indicates that the essential stimuli for exsheathment were generated and maintained under in vitro conditions of the artificial rumen. In both systems, the bicarbonate concentration and the pH reflected a similar status of the H2CO3/HCO buffer system, which is known to provide the essential stimuli for larval exsheathment of the abomasal species. These results give evidence that the RUSITEC system represents a valid system for studying the kinetics of exsheathment of strongylid nematodes under in vitro conditions. For 7 of the species investigated the obtained results represent the first data on larval exsheathment in vivo and in vitro.

scholarly journals Efficiency of the Bioverm® (Duddingtonia flagrans) fungal formulation to control in vivo and in vitro of Haemonchus contortus and Strongyloides papillosus in sheep

3 Biotech ◽  
2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Fábio Ribeiro Braga ◽  
Carolina Magri Ferraz ◽  
Edir Nepomuceno da Silva ◽  
Jackson Victor de Araújo
Keyword(s):  
Haemonchus Contortus ◽  
Duddingtonia Flagrans

scholarly journals Comparison of two in vitro methods for the detection of ivermectin resistance in Haemonchus contortus in sheep

2016 ◽  
Vol 53 (2) ◽  
pp. 120-125 ◽  
Author(s):  
M. Urda Dolinská ◽  
A. Königová ◽  
M. Babják ◽  
M. Várady
Keyword(s):  
Drug Resistance ◽  
Haemonchus Contortus ◽  
Anthelmintic Resistance ◽  
Economic Losses ◽  
Parasitic Nematodes ◽  
Migration Inhibition ◽  
In Vitro Methods ◽  
Parasitic Helminths

SummaryGastrointestinal parasitic nematodes in sheep cause severe economic losses. Anthelmintics are the most commonly used drugs for prophylaxis and therapy against parasitic helminths. The problem of drug resistance has developed for all commercially available anthelmintics in several genera and classes of helminths. In vitro and in vivo tests are used to detect anthelmintic resistance. Two in vitro methods (larval migration inhibition test and micromotility test) for the detection of ivermectin (IVM) resistance were compared using IVM-resistant and IVM-susceptible isolates of Haemonchus contortus. The degree of resistance for each test was expressed as a resistance factor (RF). The micromotility test was more sensitive for quantitatively measuring the degree of resistance between susceptible and resistant isolates. The RFs for this test for IVM and eprinomectin ranged from 1.00 to 108.05 and from 3.87 to 32.32, respectively.

scholarly journals In vitro nematicidal effect of Chenopodium ambrosioides and Castela tortuosa n-hexane extracts against Haemonchus contortus (Nematoda) and their anthelmintic effect in gerbils

2018 ◽  
Vol 93 (04) ◽  
pp. 434-439 ◽  
Author(s):  
A. Zamilpa ◽  
C. García-Alanís ◽  
M.E. López-Arellano ◽  
V.M. Hernández-Velázquez ◽  
M.G. Valladares-Cisneros ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Adult Stage ◽  
Chenopodium Ambrosioides ◽  
Anthelmintic Efficacy ◽  
Hexane Extracts ◽  
Parasitic Burden ◽  
The Individual ◽  
Anthelmintic Effect

AbstractThe in vitro nematicidal effect of Chenopodium ambrosioides and Castela tortuosa n-hexane extracts (E-Cham and E-Cato, respectively) on Haemonchus contortus infective larvae (L3) and the anthelmintic effect of these extracts against the pre-adult stage of the parasite in gerbils were evaluated using both individual and combined extracts. The in vitro confrontation between larvae and extracts was performed in 24-well micro-titration plates. The results were considered 24 and 72 h post confrontation. The in vivo nematicidal effect was examined using gerbils as a study model. The extracts from the two assessed plants were obtained through maceration using n-hexane as an organic agent. Gerbils artificially infected with H. contortus L3 were treated intraperitoneally with the corresponding extract either individually or in combination. The results showed that the highest individual lethal in vitro effect (96.3%) was obtained with the E-Cham extract at 72 h post confrontation at 40 mg/ml, followed by E-Cato (78.9%) at 20 mg/ml after 72 h. The highest combined effect (98.7%) was obtained after 72 h at 40 mg/ml. The in vivo assay showed that the individual administration of the E-Cato and E-Cham extracts reduced the parasitic burden in gerbils by 27.1% and 45.8%, respectively. Furthermore, the anthelmintic efficacy increased to 57.3% when both extracts were administered in combination. The results of the present study show an important combined nematicidal effect of the two plant extracts assessed against L3 in gerbils.

Haemonchus contortus: In vitro and in vivo anthelmintic activity of aqueous and hydro-alcoholic extracts of Hedera helix

2007 ◽  
Vol 116 (4) ◽  
pp. 340-345 ◽  
Author(s):  
T. Eguale ◽  
G. Tilahun ◽  
A. Debella ◽  
A. Feleke ◽  
E. Makonnen
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Hedera Helix

scholarly journals Anthelmintic activity of Cymbopogon citratus against Haemonchus contortus

2015 ◽  
Vol 24 (3) ◽  
pp. 268-275 ◽  
Author(s):  
Iara Tersia Freitas Macedo ◽  
Lorena Mayana Beserra de Oliveira ◽  
Wesley Lyeverton Correia Ribeiro ◽  
Jessica Maria Leite dos Santos ◽  
Kaline das Chagas Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Meriones Unguiculatus ◽  
Parasitic Nematodes ◽  
Control Group ◽  
Cymbopogon Citratus ◽  
Egg Hatching

Parasitic nematodes are of major economic importance in livestock. An alternative for the control of parasites is phytotherapy. This study evaluated the efficacy of Cymbopogon citratus decoction (CcD), C. citratus essential oil (CcEo) and citral against Haemonchus contortus using in vitro egg hatch test (EHT) and larval development test (LDT) and an in vivo test using a Meriones unguiculatus (gerbil) model. The effect of 800 mg/kg CcEo was evaluated in gerbils that had been artificially infected with 5,000 third-stage H. contortus larvae. The effective concentrations required to inhibit 50% (EC50) of egg hatching were 0.46, 0.14 and 0.13 mg/mL for CcD, CcEo and citral, respectively. The EC50 values in the LDT were 5.04, 1.92 and 1.37 mg/mL for CcD, CcEo and citral, respectively. H. contortus population in the group treated with C. citratus essential oil was reduced by 38.5% (P< 0.05) in comparison to the control group. These results suggest that it may be possible to use C. citratusessential oil to control of H. contortus parasite of small ruminant.

Terminalia catappa : Chemical composition, in vitro and in vivo effects on Haemonchus contortus

2017 ◽  
Vol 246 ◽  
pp. 118-123 ◽  
Author(s):  
L.M. Katiki ◽  
A.C.P. Gomes ◽  
A.M.E. Barbieri ◽  
P.A. Pacheco ◽  
L. Rodrigues ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Haemonchus Contortus ◽  
Terminalia Catappa ◽  
In Vivo Effects

In vitro activity of essential oils of free and nanostructured Melaleuca alternifolia and of terpinen-4-ol on eggs and larvae of Haemonchus contortus

2015 ◽  
Vol 90 (3) ◽  
pp. 377-382 ◽  
Author(s):  
T.H. Grando ◽  
M.F. de Sá ◽  
M.D. Baldissera ◽  
C.B. Oliveira ◽  
M.E. de Souza ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Gastrointestinal Nematodes ◽  
Melaleuca Alternifolia ◽  
Egg Hatching ◽  
Promising Line ◽  
New Compounds ◽  
Eggs And Larvae ◽  
Production Losses

AbstractHaemonchus contortus is one of the major gastrointestinal nematodes responsible for significant economic and production losses of sheep. Diseases caused by this species lack effective anthelmintic products, and the search for new compounds to replace synthetic anthelmintics has been extensive. The present investigation assesses the in vitro activity of the essential oil of melaleuca (Melaleuca alternifolia), both free (TTO) and nanostructured (nanoTTO), and terpinen-4-ol (terp-4-ol) on eggs and larvae of H. contortus. Tests of egg hatching (EHT) and inhibition of larval migration (LMIT) were used to assess the in vitro efficacy of TTO, nanoTTO and terp-4-ol. Using EHT, at a concentration of 3.5 mg/ml, 100% inhibition occurred using TTO and terp-4-ol, with LC50 values of 0.43 and 0.63 mg/ml, and LC90 values of 1.75 mg/ml and 3.12 mg/ml, respectively. NanoTTO had lower activity, with 82.6% inhibition at the same concentration. Using LMIT, TTO and nanoTTO had a similar activity with 88.0% and 84.8% inhibition, respectively, at a concentration of 56 mg/ml. Terp-4-ol had a greater effect on larvae, with 85.7% inhibition at a concentration of 56 mg/ml and 82.4% at 3.5 mg/ml, demonstrating high activity at the lowest concentration tested. Therefore, the results indicate that all substances tested showed ovicidal and larvicidal activity against H. contortus. TTO, terp-4-ol and, mainly, nanoTTO may be targeted in in vivo studies, besides being a promising line of research into the control and treatment of veterinary important helminths.

scholarly journals Efecto antimicrobiano in vitro de la Camellia sinensis sobre bacterias orales

2014 ◽  
Vol 10 (1) ◽  
pp. 18
Author(s):  
Hilda Moromi Nakata ◽  
Elba Martínez Cadillo ◽  
Jorge Villavicencio Gastelú ◽  
Jonny Burga Sánchez ◽  
Donald Ramos Perfecto
Keyword(s):  
Camellia Sinensis ◽  
Ft Ir ◽  
Para Determinar ◽  
Los Grupos ◽  
Estudiantes Universitarios

Con el objeto de determinar, el efecto antimicrobiano in vitro de soluciones de Camellia sinensis (te verde) de cuatro marcas comerciales (A,B,C y D), se recolectó saliva no estimulada de 40 estudiantes universitarios y se sembró en el medio de Agar Tripticasa soya. Utilizándose el metodo de difusión por discos para las soluciones de té y los controles positivos (Amoxicilina) y negativo (agua destilada), las placas se incubaron a 37 °C /24 horas. Igual procedimiento se realizó con la Cepa de S. mutans ATCC 25175. De acuerdo a los resultados las cuatro marcas de té verde produjeron halos de inhibición de crecimiento de colonias. El análisis estadístico de las cuatro marcas de té determinó que existía diferencias significativas entre las medias de las muestras. Comparando pares de medias, resultó la existencia de diferencias significativas a nivel de 0.05 entre pares de marcas AB;AC;BD;CD. Por otro lado en el análisis de las muestras de infusión de té verde para determinar la presencia de polifenoles, mediante la espectrofotometría-infrarrojo con transformada de Fourier (FT-IR), se observó picos de transmitancia en longitudes de onda para los grupos oxidrilos(-0H) y anillo aromático. De los resultados obtenidos se concluye que se ha evidenciado la acción antibacteriana para la cepa de S. mutans, asi como para la microflora mixta salival, y que existe diferencias en la acción, dependiendo de la marca utilizada. Es necesario la continuación de los estudios para comprobar su efecto antimicrobiano in vivo, para el uso como enjuagatorio bucal, así como estudios para el análisis de los principales componentes activos del té verde para la identificación de las mejores soluciones a utilizar para dicho propósito, estudios de costo/beneficio, su aceptabilidad entre usuarios, efecto sobre otros microorganismos, etc.

Sign in / Sign up

Export Citation Format

Share Document