scholarly journals Evaluation of Vascularization of Porous Calcium Phosphate by Chick Chorioallantoic Membrane Model ex vivo

2017 ◽  
Vol 32 (6) ◽  
pp. 649 ◽  
Author(s):  
XIAO Wen ◽  
LIU Yu-Mei ◽  
REN Kai-Ge ◽  
SHI Feng ◽  
LI Yan ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Ex Vivo ◽  
Chorioallantoic Membrane ◽  
Membrane Model ◽  
Chick Chorioallantoic Membrane

scholarly journals Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

2021 ◽  
Vol 12 ◽  
pp. 204173142110056
Author(s):  
Nupur Kohli ◽  
Vaibhav Sharma ◽  
Alodia Orera ◽  
Prasad Sawadkar ◽  
Nazanin Owji ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Clinical Model ◽  
Chick Chorioallantoic Membrane ◽  
Biomimetic Method ◽  
Alginate Scaffold

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

scholarly journals Antiangiogenic potential of Jatropha curcas latex in the chick chorioallantoic membrane model

2019 ◽  
Vol 29 (1) ◽  
pp. 32157
Author(s):  
Luciane Madureira Almeida ◽  
Elisa Flávia Luiz Cardoso Bailão ◽  
Illana Reis Pereira ◽  
Fabrício Alves Ferreira ◽  
Patrícia Lima D'Abadia ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Jatropha Curcas ◽  
Physicochemical Characterization ◽  
Chorioallantoic Membrane ◽  
Angiogenesis Inhibitor ◽  
Negative Control ◽  
New Drugs ◽  
Membrane Model ◽  
Chick Chorioallantoic Membrane

AIMS: To perform a physicochemical and phytochemical characterization of Jatropha curcas latex and to investigate its antiangiogenic potential. METHODS: We performed an initial physicochemical characterization of J. curcas latex using thermal gravimetric analyses and Fourier Transform Infrared spectroscopy. After that, phenols, tannins and flavonoids were quantified. Finally, the potential of J. curcas latex to inhibit angiogenesis was evaluated using the chick chorioallantoic membrane model. Five groups of 20 fertilized chicken eggs each had the chorioallantoic membrane exposed to the following solutions: (1) water, negative control; (2) dexamethasone, angiogenesis inhibitor; (3) Regederm®, positive control; (4) 25% J. curcas latex diluted in water; (5) 50% J. curcas latex diluted in water; and (6) J. curcas crude latex. Analysis of the newly-formed vascular net was made through captured images and quantification of the number of pixels. Histological analyses were performed to evaluate the inflammation, neovascularization, and hyperemia parameters. The results were statically analyzed with a significance level set at p ˂0.05.RESULTS: Physicochemical characterization showed that J. curcas latex presented a low amount of cis-1.4-polyisoprene, which reduced its elasticity and thermal stability. Phytochemical analyses of J. curcas latex identified a substantial amount of phenols, tannins, and flavonoids (51.9%, 11.8%, and 0.07% respectively). Using a chick chorioallantoic membrane assay, we demonstrated the antiangiogenic potential of J. curcas latex. The latex induced a decrease in the vascularization of the membranes when compared with neutral and positive controls (water and Regederm®). However, when compared with the negative control (dexamethasone), higher J. curcas latex concentrations showed no significant differences.CONCLUSIONS: J. curcas latex showed low thermal stability, and consisted of phenols, tannins, and flavonoids, but little or no rubber. Moreover, this latex demonstrated a significant antiangiogenic activity on a chick chorioallantoic membrane model. The combination of antimutagenic, cytotoxic, antioxidant and antiangiogenic properties makes J. curcas latex a potential target for the development of new drugs.

scholarly journals Chick chorioallantoic membrane model forin ovoevaluation of timolol maleate–brimonidine tartrate ocular inserts

Drug Delivery ◽  
2013 ◽  
Vol 21 (4) ◽  
pp. 307-314 ◽  
Author(s):  
Vinod Kombath Ravindran ◽  
Swathi Repala ◽  
Sandhya Subadhra ◽  
Ashok Kumar Appapurapu
Keyword(s):  
Chorioallantoic Membrane ◽  
Membrane Model ◽  
Timolol Maleate ◽  
Chick Chorioallantoic Membrane ◽  
Brimonidine Tartrate

Quantitation of Angiogenesis in the Chick Chorioallantoic Membrane Model Using Fractal Analysis

1996 ◽  
Vol 51 (1) ◽  
pp. 2-14 ◽  
Author(s):  
Loren M. Kirchner ◽  
Steven P. Schmidt ◽  
Brian S. Gruber
Keyword(s):  
Fractal Analysis ◽  
Chorioallantoic Membrane ◽  
Membrane Model ◽  
Chick Chorioallantoic Membrane

scholarly journals Development of a new chick chorioallantoic membrane model for human pancreas adenocarcinoma

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Vincent CASTRONOVO ◽  
Arnaud GONZALEZ ◽  
Philippe DELVENNE ◽  
Olivier PEULEN
Keyword(s):  
Chorioallantoic Membrane ◽  
Membrane Model ◽  
Human Pancreas ◽  
Chick Chorioallantoic Membrane ◽  
Pancreas Adenocarcinoma

scholarly journals Evolving applications of the egg: chorioallantoic membrane assay and ex vivo organotypic culture of materials for bone tissue engineering

2020 ◽  
Vol 11 ◽  
pp. 204173142094273
Author(s):  
Karen M Marshall ◽  
Janos M Kanczler ◽  
Richard OC Oreffo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Organotypic Culture ◽  
Ex Vivo ◽  
Chorioallantoic Membrane ◽  
Experimental Methodology ◽  
Chick Chorioallantoic Membrane ◽  
Recent Developments ◽  
Chorioallantoic Membrane Assay

The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the chorioallantoic membrane assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches, including timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo chorioallantoic membrane assay and the ex vivo organotypic culture to advance the field of bone tissue engineering, and highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

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