Development of molecular markers linkaged to Sharka (Plum pox virus, PPV) resistance inPrunus

2007 ◽  
Vol 42 (2) ◽  
pp. 223-233 ◽  
Author(s):  
P. Martínez-Gómez ◽  
P. Díaz-Vivancos ◽  
M. Rubio ◽  
M. Clemente ◽  
F. Dicenta ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Plum Pox Virus ◽  
Ppv Resistance

scholarly journals Cost-Effective and Time-Efficient Molecular Assisted Selection for Ppv Resistance in Apricot Based on ParPMC2 Allele-Specific PCR

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1292 ◽  
Author(s):  
Ángela Polo-Oltra ◽  
Carlos Romero ◽  
Inmaculada López ◽  
María Luisa Badenes ◽  
Elena Zuriaga
Keyword(s):  
Woody Species ◽  
Cost Effective ◽  
Prunus Armeniaca ◽  
Host Susceptibility ◽  
Limiting Factor ◽  
Plum Pox Virus ◽  
Breeding Programs ◽  
Specific Pcr ◽  
Allele Specific ◽  
Ppv Resistance

Plum pox virus (PPV) is the most important limiting factor for apricot (Prunus armeniaca L.) production worldwide, and development of resistant cultivars has been proven to be the best solution in the long-term. However, just like in other woody species, apricot breeding is highly time and space demanding, and this is particularly true for PPV resistance phenotyping. Therefore, marker-assisted selection (MAS) may be very helpful to speed up breeding programs. Tightly linked ParPMC1 and ParPMC2, meprin and TRAF-C homology (MATH)-domain-containing genes have been proposed as host susceptibility genes required for PPV infection. Contribution of additional genes to PPV resistance cannot be discarded, but all available studies undoubtedly show a strong correlation between ParPMC2-resistant alleles (ParPMC2res) and PPV resistance. The ParPMC2res allele was shown to carry a 5-bp deletion (ParPMC2-del) within the second exon that has been characterized as a molecular marker suitable for MAS (PMC2). Based on this finding, we propose here a method for PPV resistance selection in apricot by combining high-throughput DNA extraction of 384 samples in 2 working days and the allele-specific genotyping of PMC2 on agarose gel. Moreover, the PMC2 genotype has been determined by PCR or by using whole-genome sequences (WGS) in 175 apricot accessions. These results were complemented with phenotypic and/or genotypic data available in the literature to reach a total of 325 apricot accessions. As a whole, we conclude that this is a time-efficient, cost-effective and straightforward method for PPV resistance screening that can be highly useful for apricot breeding programs.

scholarly journals Genetics of resistance to Plum pox virus in apricot

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. 180-182 ◽  
Author(s):  
B. Krška ◽  
J. Salava ◽  
J. Polák ◽  
P. Komínek
Keyword(s):  
Czech Republic ◽  
Point Of View ◽  
Plum Pox Virus ◽  
Genetics Of Resistance ◽  
The Czech Republic ◽  
Complementary Genes ◽  
Breeding Programmes ◽  
Ppv Resistance ◽  
Resistance Inheritance

Plum pox virus (PPV) causes serious damage in apricots grown in the Czech Republic and other countries where it is<br />present. The virus spreads in orchards from infected trees by aphids to healthy trees of susceptible cultivars. Chemical<br />control is ineffective from epidemiological point of view. For this reason growing of resistant apricot cultivars is the only<br />way how to solve one of the most significant phytopathological problem. To study PPV resistance in apricot, three crosses<br />between an apricot cultivars or a selection resistant to PPV and an apricot cultivars or a selection susceptible to PPV<br />(LE-3218 × Stark Early Orange, LE-3241 × Vestar and LE-3246 × Vestar) were performed at Faculty of Horticulture<br />of Mendel University of Agriculture and Forestry in Lednice na Moravě in 1999. The BC1 seeds were stratified and the<br />subsequent seedlings were grown in a greenhouse. The seedlings were repeatedly inoculated with PPV-Vegama isolate<br />(PPV-M strain) by an infected chip. The resistance of the plants was evaluated by symptom observing and ELISA in<br />three consecutive growth periods. The χ<sup>2</sup> test was used to analyse the data. It was found that two independent dominant<br />complementary genes conditioned PPV resistance in apricot. The significance of these findings in relation to other reports<br />is discussed. Knowledge of PPV resistance inheritance will help in planning apricot breeding programmes.

scholarly journals Inheritance of resistance to Plum pox virus in the progeny of the apricot cv. ‘Harlayne’

2009 ◽  
Vol 57 (5) ◽  
pp. 243-250 ◽  
Author(s):  
Petra Pilařová ◽  
Boris Krška
Keyword(s):  
Prunus Persica ◽  
Natural Resistance ◽  
Plum Pox Virus ◽  
Inheritance Of Resistance ◽  
Sources Of Resistance ◽  
Growing Seasons ◽  
Ppv Resistance ◽  
Dominant Genes ◽  
Sharka Disease ◽  
Leaf Symptoms

Natural resistance toPlum pox virus(PPV), the agent of sharka disease, is one of the most important traits of interest to stone fruit breeders, although few sources of resistance have been identified. One of the few apricot cultivars which does show resistance, ‘Harlayne’, was chosen for a study of the genetics of PPV resistance. It was crossed with three different cultivars, two susceptible (‘Vestar’ and ‘Strepet’) and one immune (‘Orangered’). Four different lines (since there was one reciprocal combination) were established and the F1 crosses were subsequently inoculated with the PPV-M and PPV-D strains by grafting infected buds. A woody indicatorPrunus persica‘GF 305’ was then also top-grafted onto the plants of three of these F1 populations. The observations of leaf symptoms and accompanying ELISA tests were performed over three, or in one case five, growing seasons and then hybrids were classified accordingly, as either resistant or susceptible. The resistant : susceptible ratios were calculated and compared with expected theoretical ratios using the χ2-test. The ratios of resistant to susceptible plants in the progeny derived from the four apricot crosses are compatible with the hypothesis of three dominant genes being responsible for PPV resistance, with ‘Harlayne’ being heterozygous for all three genes. However, the possibility that resistance is controlled by just two dominant genes can not be ruled out just yet.

Investigation of resistance of apricot progeny to Plum pox virus through molecular markers

2017 ◽  
pp. 25-30
Author(s):  
S. Yalçın Ateş ◽  
M. Gazel ◽  
C. Ulubaş Serçe ◽  
B.M. Asma ◽  
K. Çağlayan
Keyword(s):  
Molecular Markers ◽  
Plum Pox Virus

scholarly journals Allelic variation of simple sequence repeats markers linked to PPV resistance in Chinese apricot

2017 ◽  
Vol 44 (No. 1) ◽  
pp. 6-13
Author(s):  
Qiu-ping Zhang ◽  
Wei-sheng Liu ◽  
Ning Liu ◽  
Yu-ping Zhang ◽  
Ming Xu
Keyword(s):  
Simple Sequence Repeats ◽  
Allelic Variation ◽  
Prunus Armeniaca ◽  
Plum Pox Virus ◽  
Germplasm Resources ◽  
Prunus Mume ◽  
Ssr Primers ◽  
Ppv Resistance ◽  
High Level ◽  
Simple Sequence

Apricot is one of the oldest fruit tree crops in China and it was spread via Armenia to other areas. There are about ten species of apricot (Subg. Armeniaca Mill.) worldwide, among which nine species are native to China. Sharka disease caused by the Plum pox virus (PPV) is widely distributed in the main producing regions of apricot. In this study, linked simple sequence repeats (SSR) primers were used to detect allele variations potentially associated with PPV resistance among Chinese apricot germplasm resources, including 52 accessions belonging to Prunus armeniaca, 7 to Prunus mandshurica, 6 to Prunus sibirica, 4 to Prunus mume, 17 to other species or types. The allelic variation at loci with PPV resistance showed that these SSR markers linked to PPV resistance kept a relatively high level of diversity in Chinese apricot. The special alleles and genotypes only found in South China cultivars might reveal new PPV resistance sources. Some famous local cultivars of Chinese apricot might be considered as candidates for PPV resistance.  

Sign in / Sign up

Export Citation Format

Share Document