scholarly journals Effects of steroids on the morphology and proliferation of canine and equine mesenchymal stem cells of adipose origin — in vitro research

2014 ◽  
Vol 62 (3) ◽  
pp. 317-333 ◽  
Author(s):  
Krzysztof Marycz ◽  
Agnieszka Śmieszek ◽  
Jakub Grzesiak ◽  
Jakub Nicpoń
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Medical Practice ◽  
Proliferation Rate ◽  
Cellular Activity ◽  
Simultaneous Application ◽  
Steroid Injections ◽  
Adult Mesenchymal Stem Cells ◽  
Steroid Drugs

Disorders of the locomotive system, especially those occurring due to degenerative changes of the joints, are serious problems in daily veterinary medical practice. Steroid injections are the main way of treating these disorders. However, this approach brings usually only temporary effects of pain relief, and may cause many side effects. Alternative therapies focus on regeneration of damaged tissue using adult mesenchymal stem cells (MSCs). Since 2002, the great plasticity and immunomodulatory properties of MSCs isolated from adipose tissue (AdMSCs) have been used successfully in the treatment of degenerative joint diseases (DJD) of both dogs and horses. Possible simultaneous application of steroid therapy and stem cell transplantation could improve the commonly used clinical procedure. In this paper, the influence of the two steroid drugs (betamethasone and methylprednisolone) on AdMSCs was evaluated on the basis of morphology and proliferation rate. Both steroids positively influenced the viability and proliferation state of cells in a concentration of 0.01 mg/ml and 0.1 mg/ml, respectively. However, the concentration of 1 mg/ml had a cytotoxic effect. Moreover, the lower dosage of steroid drugs used in the experiment did not affect the morphology of cells and significantly increased cellular activity. In conclusion, our data demonstrate the stimulating effect of steroid drugs on cell morphology, proliferation rate and cytophysiological activity. These findings may influence the use of stem cells and steroids in applied regenerative veterinary medical practice in the future.

scholarly journals Long-term In vitro Expansion Alters the Biology of Adult Mesenchymal Stem Cells

2008 ◽  
Vol 68 (11) ◽  
pp. 4229-4238 ◽  
Author(s):  
Reza Izadpanah ◽  
Deepak Kaushal ◽  
Christopher Kriedt ◽  
Fern Tsien ◽  
Bindiya Patel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adult Mesenchymal Stem Cells ◽  
In Vitro Expansion

In vitro behaviour of adult mesenchymal stem cells seeded on a bioactive glass ceramic in the SiO2–CaO–P2O5 system

2008 ◽  
Vol 4 (4) ◽  
pp. 1104-1113 ◽  
Author(s):  
L. Meseguer-Olmo ◽  
A. Bernabeu-Esclapez ◽  
E. Ros-Martinez ◽  
S. Sánchez-Salcedo ◽  
S. Padilla ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bioactive Glass ◽  
Glass Ceramic ◽  
Adult Mesenchymal Stem Cells ◽  
P2o5 System

scholarly journals MicroRNA-Enriched Exosomes from Different Sources of Mesenchymal Stem Cells Can Differentially Modulate Functions of Immune Cells and Neurogenesis

Biomedicines ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 69
Author(s):  
Naina Soni ◽  
Suchi Gupta ◽  
Surender Rawat ◽  
Vishnu Krishnakumar ◽  
Sujata Mohanty ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Expression Profile ◽  
Immune Cells ◽  
Biologically Active ◽  
Rna Seq ◽  
Adult Mesenchymal Stem Cells ◽  
Neutrophil Survival ◽  
Different Sources

Adult Mesenchymal stem cells-derived exosomes carry several biologically active molecules that play prominent roles in controlling disease manifestations. The content of these exosomes, their functions, and effect on the immune cells may differ depending on their tissue sources. Therefore, in this study, we purified the exosomes from three different sources and, using the RNA-Seq approach, highly abundant microRNAs were identified and compared between exosomes and parental cells. The effects of exosomes on different immune cells were studied in vitro by incubating exosomes with PBMC and neutrophils and assessing their functions. The expression levels of several miRNAs varied within the different MSCs and exosomes. Additionally, the expression profile of most of the miRNAs was not similar to that of their respective sources. Exosomes isolated from different sources had different abilities to induce the process of neurogenesis and angiogenesis. Moreover, these exosomes demonstrated their varying effect on PBMC proliferation, neutrophil survival, and NET formation, highlighting their versatility and broad interaction with immune cells. The knowledge gained from this study will improve our understanding of the miRNA landscape of exosomes from hMSCs and provide a resource for further improving our understanding of exosome cargo and their interaction with immune cells.

scholarly journals Influence of Adult Mesenchymal Stem Cells on In Vitro Vascular Formation

2009 ◽  
Vol 15 (7) ◽  
pp. 1751-1761 ◽  
Author(s):  
J. Michael Sorrell ◽  
Marilyn A. Baber ◽  
Arnold I. Caplan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adult Mesenchymal Stem Cells ◽  
Vascular Formation

scholarly journals RAT BONE MARROW MESENCHYMAL STEM CELLS ISOLATION, CULTIVATION AND DIFFERENTIATION

2009 ◽  
Vol 15 (1) ◽  
pp. 30-34
Author(s):  
Emoke PALL ◽  
Ioan GROZA ◽  
Olga SORITAU ◽  
Ciprian TOMULEASA ◽  
Mihai CENARIU ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Marrow Aspiration ◽  
Homogeneous Population ◽  
Adult Rat ◽  
Adult Mesenchymal Stem Cells ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

Bone marrow stromal cells (MSCs) represent a heterogeneous population derived from the non–blood-forming fraction of bone marrow that regulates hematopoietic cell development. In vitro, adult mesenchymal stem cells resident in this bone marrow fraction differentiate into bone, cartilage, and fat. Because MSCs can be easily obtained using a simple bone marrow aspiration and show extensive capacity for expansion in vitro, these cells have been considered as candidates for cell therapy. The aim of this study was to purify rat MSCs from adult bone marrow and to functionally characterise their abilities to differentiate along diverse lineages. Our data demonstrate that we successfully isolated, culture-expanded and differentiated a relatively homogeneous population of MPCs from adult rat bone marrow.

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